| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,826 | C→T | 100% | S60S (TCC→TCT) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,826 | 0 | C | T | 98.9% | 261.4 / ‑4.2 | 87 | S60S (TCC→TCT) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (48/38); minor base A (0/1); total (48/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCTGTTTAATACCGGGCGCGATCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCT > AP009048/3824760‑3824892 | gCTGTTTAATACCGGGCGCGACCTGGCACTGTTATGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCa < 1:1043450/71‑1 (MQ=255)gCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGTCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCa < 1:2895270/71‑1 (MQ=255) tGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt > 1:3305842/1‑71 (MQ=255) ttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAg > 1:94957/1‑71 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt > 1:4374833/1‑71 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt > 1:3285295/1‑71 (MQ=255) aTACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGtt < 1:4281109/70‑1 (MQ=255) aTACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTc > 1:206068/1‑71 (MQ=255) tACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGa > 1:4438409/1‑66 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGa > 1:2782653/1‑70 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGa > 1:2788190/1‑70 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt < 1:117839/71‑1 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt > 1:2158296/1‑71 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt < 1:3404113/71‑1 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt > 1:4070336/1‑71 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt > 1:4139143/1‑71 (MQ=255) ggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctg > 1:2845918/1‑71 (MQ=255) ggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATc > 1:4364819/1‑69 (MQ=255) gcgcGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt < 1:457459/67‑1 (MQ=255) cgcgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgc > 1:1499247/1‑70 (MQ=255) gcgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgctg > 1:3535733/1‑71 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:3858881/2‑71 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:3240178/2‑71 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:1984867/2‑71 (MQ=255) tGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGtt > 1:3754040/1‑67 (MQ=255) ggCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTgg > 1:1094897/1‑69 (MQ=255) ggCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCAgg < 1:324476/45‑1 (MQ=38) gCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGt > 1:2279917/1‑64 (MQ=255) gCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGccc > 1:1005310/1‑71 (MQ=255) gCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGc < 1:822305/69‑1 (MQ=255) cACTGTTAGGCCGTTTTTACGCTGAAGCTTTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCt < 1:2045673/71‑1 (MQ=255) aCTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGttt > 1:2542665/1‑64 (MQ=255) aCTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTg > 1:2469317/1‑71 (MQ=255) aCTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGCCATTGAGTTCGATCTGCTGTTTGGCCCTg < 1:2698616/71‑1 (MQ=255) cTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt < 1:4439147/45‑1 (MQ=38) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGcc > 1:3743949/1‑66 (MQ=255) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:3912772/1‑71 (MQ=255) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:1626164/1‑71 (MQ=255) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:4525788/1‑71 (MQ=255) gTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGt < 1:3533710/59‑1 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt > 1:1678535/1‑51 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGc > 1:3627518/1‑68 (MQ=255) atAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTgg > 1:4006884/2‑38 (MQ=37) tAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt > 1:408364/1‑41 (MQ=38) tAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTa > 1:2458549/1‑70 (MQ=255) tAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTa > 1:3367309/1‑70 (MQ=255) aGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCg < 1:4322680/47‑1 (MQ=39) aGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTAc > 1:1565679/1‑70 (MQ=255) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACa < 1:4333436/70‑1 (MQ=255) gCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt < 1:1034522/42‑1 (MQ=38) gCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACaaa < 1:2424188/71‑1 (MQ=255) cGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAgg > 1:14063/1‑71 (MQ=255) gTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt > 1:1697544/1‑44 (MQ=38) gTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAggg < 1:1536608/71‑1 (MQ=255) gTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAggg < 1:343236/71‑1 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGc < 1:941929/59‑1 (MQ=255) tttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAg > 1:2174700/1‑66 (MQ=255) tttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGAt < 1:4330933/70‑1 (MQ=255) tACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATcc < 1:2487395/70‑1 (MQ=255) aCGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:941734/1‑48 (MQ=39) aCGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATccc > 1:3612353/1‑70 (MQ=255) aCGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATccc < 1:3647606/70‑1 (MQ=255) cGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGTATCCCGa < 1:2673844/71‑1 (MQ=255) cGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGa < 1:535655/71‑1 (MQ=255) gCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGa < 1:1258216/70‑1 (MQ=255) gCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGAt < 1:2179273/71‑1 (MQ=255) tGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTgg < 1:1583131/45‑1 (MQ=38) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGc < 1:4045436/70‑1 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGc > 1:1197125/1‑70 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGc < 1:1900282/70‑1 (MQ=255) gcattGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcgac < 1:1600689/68‑1 (MQ=255) cattGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcgacc < 1:732104/69‑1 (MQ=255) attGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAgg > 1:3771047/2‑54 (MQ=255) attGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcgacca > 1:4037447/2‑71 (MQ=255) ttGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCgaccac < 1:3313188/71‑1 (MQ=255) ttGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCgaccac < 1:2469207/71‑1 (MQ=255) tggtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACaaa > 1:3942010/1‑50 (MQ=255) ggtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGAt > 1:2768310/1‑54 (MQ=255) ggtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcgacca > 1:4353015/1‑68 (MQ=255) ggtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCgaccact < 1:1173327/70‑2 (MQ=255) gtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCgaccacta < 1:2385354/70‑3 (MQ=255) ggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTAc < 1:2013871/43‑1 (MQ=255) ggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcgacca > 1:4082331/1‑65 (MQ=255) ggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGc < 1:2055809/60‑1 (MQ=255) gATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGa < 1:2321605/55‑1 (MQ=255) aTTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGcc > 1:1773308/1‑70 (MQ=255) ttCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGa < 1:1827400/53‑1 (MQ=255) | GCTGTTTAATACCGGGCGCGATCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCT > AP009048/3824760‑3824892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |