| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,781 | T→C | 100% | D45D (GAT→GAC) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,781 | 0 | T | C | 100.0% | 183.1 / NA | 52 | D45D (GAT→GAC) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (33/19); total (33/19) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTACGCTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGATCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTG > AP009048/3824714‑3824847 | ttACGCTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTg > 1:4468875/1‑71 (MQ=255) aCGCTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTgg > 1:816967/1‑70 (MQ=255) cGCTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGc < 1:3553845/70‑1 (MQ=255) cGCTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCa < 1:2528581/71‑1 (MQ=255) aaaTCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTg < 1:2753503/69‑1 (MQ=255) aaaTCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGtt < 1:232781/71‑1 (MQ=255) tCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCt > 1:463653/1‑59 (MQ=255) aaaGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTAc < 1:1256218/71‑1 (MQ=255) aaaGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTAc > 1:2491670/1‑71 (MQ=255) aaGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTgg > 1:1151334/1‑50 (MQ=255) cccTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGtttt < 1:2834804/63‑1 (MQ=255) cTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTa > 1:2985376/1‑63 (MQ=255) cTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTTAAg > 1:3955612/1‑71 (MQ=255) cTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAg > 1:180854/1‑71 (MQ=255) aTTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGca > 1:3872667/1‑70 (MQ=255) aTTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGca > 1:2749721/1‑70 (MQ=255) aTTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGca > 1:2986863/1‑70 (MQ=255) cttcAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtgg > 1:3666996/1‑71 (MQ=255) ttcCACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGcat > 1:262544/1‑65 (MQ=255) cAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtgg < 1:2297095/68‑1 (MQ=255) aaCGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGt > 1:1222344/1‑40 (MQ=255) aCGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGAt > 1:4027081/1‑71 (MQ=255) gCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGtt > 1:4608994/1‑38 (MQ=255) gCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTc > 1:705966/1‑71 (MQ=255) gCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTc < 1:3542914/71‑1 (MQ=255) gCTGTTTAATACCGGGCGCGACCTGGCACTGTTATGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCa < 1:1043450/71‑1 (MQ=255) gCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGTCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCa < 1:2895270/71‑1 (MQ=255) cTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAg > 1:4426887/1‑52 (MQ=255) tGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGcat < 1:2383614/54‑3 (MQ=255) tGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt > 1:3305842/1‑71 (MQ=255) tttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGca < 1:3158301/51‑2 (MQ=255) ttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAg > 1:94957/1‑71 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt > 1:3285295/1‑71 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt > 1:4374833/1‑71 (MQ=255) aaTACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTa < 1:2217807/38‑1 (MQ=255) aTACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGtt < 1:4281109/70‑1 (MQ=255) aTACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTc > 1:206068/1‑71 (MQ=255) tACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGa > 1:4438409/1‑66 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtgg < 1:1567726/48‑1 (MQ=39) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGa > 1:2788190/1‑70 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGa > 1:2782653/1‑70 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt > 1:2158296/1‑71 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt > 1:4070336/1‑71 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt > 1:4139143/1‑71 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt < 1:117839/71‑1 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt < 1:3404113/71‑1 (MQ=255) ggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctg > 1:2845918/1‑71 (MQ=255) ggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATc > 1:4364819/1‑69 (MQ=255) gcgcGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt < 1:457459/67‑1 (MQ=255) cgcgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGa < 1:1256603/47‑1 (MQ=39) cgcgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgc > 1:1499247/1‑70 (MQ=255) gcgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgctg > 1:3535733/1‑71 (MQ=255) | TTACGCTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGATCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTG > AP009048/3824714‑3824847 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |