Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 3,685,267 C→T 100% pseudogene (855/1645 nt)
pseudogene (855/984 nt)
ilvG ←
ilvG ←
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0090483,685,2670CT100.0% 164.2 / NA 57pseudogene (855/1645 nt)
pseudogene (855/984 nt)
ilvG
ilvG
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/30);  total (27/30)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CATGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGCGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGCAGGTCAC  >  AP009048/3685200‑3685334
                                                                   |                                                                   
caTGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTTGCGTGTGgtgc                                                                   >  1:1665259/1‑70 (MQ=255)
caTGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGgtgc                                                                   >  1:442808/1‑70 (MQ=255)
caTGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGtgcg                                                                  <  1:242852/71‑1 (MQ=255)
 aTGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGtgcg                                                                  >  1:1134575/1‑70 (MQ=255)
 aTGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGtgcg                                                                  >  1:3558351/1‑70 (MQ=255)
      ccTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGgtg                                                            <  1:4144237/71‑1 (MQ=255)
         gACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGtgcg                                                                  <  1:3762463/62‑1 (MQ=255)
          aCGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGtt                                                          >  1:3922297/1‑69 (MQ=255)
           cGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAg                                                       <  1:1493121/71‑1 (MQ=255)
            gCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGt                                                      <  1:3693412/71‑1 (MQ=255)
            gCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGt                                                      >  1:3690951/1‑71 (MQ=255)
              aGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGttt                                                    <  1:1177358/71‑1 (MQ=255)
              aGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGttt                                                    >  1:486876/1‑71 (MQ=255)
              aGCTTGTTCATTTATGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGttt                                                    >  1:1609988/1‑71 (MQ=255)
                  tGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCg                                                >  1:371996/1‑71 (MQ=255)
                    ttCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGt                                                      <  1:4303203/63‑1 (MQ=255)
                      cATTTCTGCCTGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGtt                                                          <  1:2952638/57‑1 (MQ=255)
                      cATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTc                                             <  1:2859836/70‑1 (MQ=255)
                      cATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCa                                            >  1:4061275/1‑71 (MQ=255)
                       aTTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGc                                                  <  1:741899/64‑1 (MQ=255)
                       aTTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCAc                                           <  1:3927957/71‑1 (MQ=255)
                        tttCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCAcc                                          <  1:4550953/71‑1 (MQ=255)
                          tCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTc                                                         >  1:203093/1‑54 (MQ=255)
                          tCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCAccc                                         >  1:3030501/1‑70 (MQ=255)
                           cTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCgg                                       >  1:4439968/1‑71 (MQ=255)
                               cGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGttt                                                    <  1:73514/54‑1 (MQ=255)
                               cGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGtcat                                   <  1:1213833/71‑1 (MQ=255)
                                 ggTCGATATCCATATGGATAACACTGGCGTGTGGTGCGa                                                                 <  1:442399/39‑1 (MQ=255)
                                 ggTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGtcatca                                 <  1:894953/71‑1 (MQ=255)
                                   tCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaaa                               <  1:159531/71‑1 (MQ=255)
                                    cGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGttt                                                    >  1:2593787/1‑49 (MQ=255)
                                     gATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCg                                                <  1:86721/52‑1 (MQ=255)
                                     gATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAAc                             >  1:1288026/1‑71 (MQ=255)
                                     gATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAAc                             >  1:227846/1‑71 (MQ=255)
                                       tatCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGtt                                                          <  1:1906381/40‑1 (MQ=255)
                                       tatCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGt                           >  1:2585277/1‑71 (MQ=255)
                                        atCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTg                          >  1:1825821/1‑71 (MQ=255)
                                         tCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTg                          <  1:3329572/70‑1 (MQ=255)
                                          ccATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAg                                                       <  1:4148107/40‑1 (MQ=255)
                                          ccATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGtcat                                   >  1:869971/1‑60 (MQ=255)
                                             tatGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCAcc                                          >  1:69116/1‑50 (MQ=255)
                                             tatGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaaa                               <  1:4456701/61‑1 (MQ=255)
                                              atGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaa                                >  1:2819752/1‑59 (MQ=255)
                                              atGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCa                    >  1:2655023/1‑71 (MQ=255)
                                               tGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGttt                                                    >  1:1833624/1‑38 (MQ=255)
                                                 gATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACg                  <  1:3716314/70‑1 (MQ=255)
                                                   tAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGc                >  1:2465722/1‑70 (MQ=255)
                                                   tAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCg               <  1:2348951/71‑1 (MQ=255)
                                                     acacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGtc                                     >  1:2804817/1‑47 (MQ=255)
                                                     acacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGAt             >  1:974088/1‑71 (MQ=255)
                                                      cacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGAt             >  1:2482841/1‑70 (MQ=255)
                                                       acTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGAt             >  1:724582/1‑69 (MQ=255)
                                                       acTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATca           <  1:386959/71‑1 (MQ=255)
                                                        cTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTc                                             >  1:712373/1‑36 (MQ=255)
                                                         tGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaa                                <  1:4453247/48‑1 (MQ=255)
                                                           gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc         <  1:626850/69‑1 (MQ=255)
                                                           gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc         <  1:2655436/69‑1 (MQ=255)
                                                           gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc         <  1:3915749/69‑1 (MQ=255)
                                                            cGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagcaag      <  1:1513324/71‑3 (MQ=39)
                                                              tgtgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTc    <  1:2247117/71‑1 (MQ=39)
                                                                tgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcgc  <  1:2554906/71‑3 (MQ=38)
                                                                tgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcg   >  1:4183968/1‑69 (MQ=38)
                                                                tgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcg   >  1:970183/1‑69 (MQ=38)
                                                                   |                                                                   
CATGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGCGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGCAGGTCAC  >  AP009048/3685200‑3685334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: