| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,685,100 | A→C | 100% | pseudogene (1022/1645 nt) | ilvG ← | ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,685,100 | 0 | A | C | 100.0% | 317.5 / NA | 90 | pseudogene (1022/1645 nt) | ilvG | ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (36/54); total (36/54) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGATCCGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGAATGTTCATCACGCAGCTGCGCGCAGTGTTGCTGCCAGTCATTG‑‑ATTTAACGGCTGCTGTAATGCT > AP009048/3685033‑3685165 | cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCTTAACGCCAGGCATg > 1:2027726/1‑71 (MQ=255)cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATg < 1:454582/71‑1 (MQ=255)cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATg < 1:4391037/71‑1 (MQ=255) tctgACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGtt > 1:4571949/4‑70 (MQ=255) tctgACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGtt > 1:2785058/4‑70 (MQ=255) tctgACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGt < 1:913763/66‑1 (MQ=255) aCAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGtt < 1:2778566/66‑1 (MQ=255) aCAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGt < 1:997049/65‑1 (MQ=255) aCAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTtcatca < 1:4390881/71‑1 (MQ=255) aGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACg > 1:3351536/1‑71 (MQ=255) gttgttgttAAAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCTTAACGCCAGGCATGTtc > 1:1059530/1‑64 (MQ=255) gttgttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGc < 1:1143896/71‑1 (MQ=255) gttgttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGc < 1:2521846/71‑1 (MQ=255) ttgttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGc < 1:785143/70‑1 (MQ=255) ttgttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCa < 1:1343542/71‑1 (MQ=255) gttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAg < 1:3194294/70‑1 (MQ=255) gttTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAg < 1:1404407/70‑1 (MQ=255) ttttAACAACAACGGCGCTTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTtcatc < 1:3917458/63‑1 (MQ=255) tttAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTAATCACGCAGCTg > 1:757491/1‑71 (MQ=255) ttAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCt > 1:819346/1‑69 (MQ=255) tAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCGTGTTCATCACGCAGCTgcg > 1:1940444/1‑71 (MQ=255) tAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTgc < 1:4265596/70‑1 (MQ=255) aacaacaacGGGGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGt > 1:243562/1‑54 (MQ=39) aacaacaacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGc < 1:1578372/66‑1 (MQ=255) acaacaacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGtt > 1:1470358/1‑54 (MQ=255) acaacaacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTtcat < 1:3736783/57‑1 (MQ=255) caacaacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTgcgcgc < 1:1459225/71‑1 (MQ=255) caacaacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTgcgcgc < 1:1527526/71‑1 (MQ=255) caacaacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTgcgcgc < 1:3393144/71‑1 (MQ=255) caacaacGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTgcgcgc < 1:2044544/71‑1 (MQ=255) ggCGCGTAGATAGCGTCACCGGGATGGTCGTGACGCCAGGCATCTTCATCACGc > 1:1944462/1‑54 (MQ=38) cgcgTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTtcat < 1:4148423/47‑1 (MQ=255) gcgTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTtcat < 1:770192/46‑1 (MQ=255) gTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTagctgc > 1:740035/1‑70 (MQ=255) gTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTagctgc > 1:1826764/1‑70 (MQ=255) gTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTagctg > 1:2018711/1‑69 (MQ=255) gTAGATAGCGTCACCGGGATGGTCGTAACACCAGGCATGTtcatca > 1:3445311/1‑46 (MQ=38) tAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAgtgta < 1:987145/64‑2 (MQ=255) tAGATAGCGTCACC‑GGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTagctgc < 1:4170280/68‑1 (MQ=38) aTAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTtca < 1:3953051/39‑1 (MQ=255) aTAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTgcgcg < 1:442823/54‑1 (MQ=255) aTAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAgtgtag > 1:3378111/1‑60 (MQ=255) aTAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAgtgta < 1:3653769/61‑2 (MQ=255) aTAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAgtg < 1:1505569/59‑1 (MQ=255) aTAGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTc < 1:665696/71‑1 (MQ=255) aGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCAt > 1:4362620/1‑71 (MQ=255) aGCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCAt > 1:1571017/1‑71 (MQ=255) gCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCa < 1:345578/53‑1 (MQ=255) gCGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCAtt > 1:827736/1‑71 (MQ=255) cGTCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTg < 1:4099834/71‑1 (MQ=255) tCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCa < 1:1733752/50‑1 (MQ=255) tCACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTG‑‑a > 1:4281282/1‑70 (MQ=255) cACCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTgcgcg < 1:1953812/47‑1 (MQ=255) aCCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCa > 1:3476554/1‑48 (MQ=255) aCCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCa > 1:2404359/1‑60 (MQ=39) aCCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTG‑‑Atat < 1:1246993/71‑3 (MQ=255) aCCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTG‑‑Atat > 1:192749/1‑69 (MQ=255) aCCGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTG‑‑Atat < 1:716092/71‑3 (MQ=255) ccGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAgtgta < 1:1219064/52‑2 (MQ=39) ccGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTG‑‑Atatt > 1:4390536/1‑68 (MQ=255) ccGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTG‑‑Atatt < 1:1931482/71‑4 (MQ=255) ccGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTG‑‑Atatt < 1:1112720/71‑4 (MQ=255) cGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTagct < 1:2611008/54‑1 (MQ=38) cGGGATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTG‑‑Atatt > 1:3540087/1‑67 (MQ=255) gggATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTagc > 1:2848333/1‑52 (MQ=38) ggATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCa > 1:3395304/1‑44 (MQ=255) gATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCa < 1:3571337/43‑1 (MQ=255) gATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCa < 1:2512536/43‑1 (MQ=255) gATGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCa < 1:1493705/59‑1 (MQ=39) aTGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCa > 1:1126990/1‑54 (MQ=38) tGGTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTc < 1:711469/56‑1 (MQ=38) gTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCa < 1:1640152/55‑1 (MQ=38) gTCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGgc < 1:164982/70‑1 (MQ=255) tCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAg < 1:3557677/51‑1 (MQ=38) tCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGgctg > 1:2677276/1‑71 (MQ=255) tCGTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGgc > 1:4376475/1‑69 (MQ=37) agTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCAtt > 1:1106939/2‑55 (MQ=38) gTAACGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGgctgc > 1:1842454/1‑70 (MQ=255) aCGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGGCTGCTGt < 1:4076415/70‑1 (MQ=255) aCGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGGCTGCTGt < 1:1903212/70‑1 (MQ=255) aCGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGGCTGCTGt < 1:4310909/70‑1 (MQ=255) aCGCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGGCTGCTGTa < 1:3965753/71‑1 (MQ=255) gCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGGCTGCTGTAAt < 1:280267/71‑1 (MQ=255) gCCAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGGCTGCTGTAAt > 1:4396372/1‑71 (MQ=255) ccAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGGCTGCTGTAATg > 1:2369963/1‑71 (MQ=255) cAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGGCTGCTGTAATGc < 1:1047333/71‑1 (MQ=255) cAGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGGCTGCTGTAATGc > 1:3497322/1‑71 (MQ=255) aGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGGCTGCTGTAATGc < 1:2197272/70‑1 (MQ=255) aGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGGCTGCTGTAATGCt > 1:668113/1‑71 (MQ=255) aGGCATGTTCATCACGCAGCTGCGCGCAGTGTAGCTGCCAGTCATTGATATTTAACGGCTGCTGTAATGCt < 1:1984075/71‑1 (MQ=255) | CGATCCGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGAATGTTCATCACGCAGCTGCGCGCAGTGTTGCTGCCAGTCATTG‑‑ATTTAACGGCTGCTGTAATGCT > AP009048/3685033‑3685165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |