Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 3,685,038 C→T 100% pseudogene (21/582 nt)
pseudogene (1084/1645 nt)
ilvG ←
ilvG ←
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0090483,685,0380CT100.0% 315.7 / NA 103pseudogene (21/582 nt)
pseudogene (1084/1645 nt)
ilvG
ilvG
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (57/46);  total (57/46)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 103 total aligned reads displayed.

TGCTGCGCAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCCGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGAATG  >  AP009048/3684970‑3685103
                                                                    |                                                                 
tgctgcGCAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGa                                                                 >  1:2998658/1‑71 (MQ=255)
   tgcGCAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAg                                                              >  1:2547370/1‑71 (MQ=255)
     cgcAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAg                                                              <  1:4160898/69‑1 (MQ=255)
     cgcAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAg                                                              <  1:3535742/69‑1 (MQ=255)
      gcAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttg                                                           <  1:1635628/71‑1 (MQ=255)
      gcAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttg                                                           >  1:1154118/1‑71 (MQ=255)
      gcAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttg                                                           >  1:2588359/1‑71 (MQ=255)
          cccACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGttt                                                        >  1:2617332/1‑70 (MQ=255)
           ccACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTa                                                      <  1:1396871/71‑1 (MQ=255)
           ccACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTa                                                      <  1:1179907/71‑1 (MQ=255)
            cacaTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaa                                                     <  1:3664558/71‑1 (MQ=255)
            cacaTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaa                                                     >  1:3579076/1‑71 (MQ=255)
             acaTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaa                                                     >  1:352703/1‑70 (MQ=255)
             acaTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaa                                                     >  1:923870/1‑70 (MQ=255)
              caTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACa                                                               >  1:1804113/1‑59 (MQ=255)
              caTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttgt                                                          <  1:1178439/64‑1 (MQ=255)
              caTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaaca                                                   <  1:2597014/71‑1 (MQ=255)
              caTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaaca                                                   <  1:2602534/71‑1 (MQ=255)
              caTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaac                                                    >  1:2190622/1‑70 (MQ=255)
                tCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaa                                                  >  1:4115306/1‑70 (MQ=255)
                tCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaa                                                  >  1:3505402/1‑70 (MQ=255)
                 cTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaac                                                 >  1:1612629/1‑70 (MQ=255)
                  tGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaacaa                                               >  1:4279338/1‑71 (MQ=255)
                    gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaacaa                                               <  1:770569/69‑1 (MQ=255)
                    gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaaca                                                <  1:1961096/68‑1 (MQ=255)
                    gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACg                                             <  1:1655960/71‑1 (MQ=255)
                     tgctgcCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttgtt                                                         <  1:175977/58‑1 (MQ=255)
                     tgctgcCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaaca                                                <  1:3522682/67‑1 (MQ=255)
                     tgctgcCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACg                                             <  1:2106839/70‑1 (MQ=255)
                       ctgcCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGAc                                                                >  1:1491647/1‑49 (MQ=255)
                       ctgcCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaa                                                  >  1:1415774/1‑63 (MQ=255)
                        tgcCCCACATCTGTTGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaac                                                 <  1:1805879/63‑1 (MQ=255)
                        tgcCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGgcgc                                         >  1:2500541/1‑71 (MQ=255)
                          ccccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaa                                                     <  1:3684604/57‑1 (MQ=255)
                          ccccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGcgcg                                        >  1:204249/1‑70 (MQ=255)
                          ccccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGcgcg                                        <  1:2894636/70‑1 (MQ=255)
                          ccccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGcgcg                                        <  1:863240/70‑1 (MQ=255)
                            ccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGcgcg                                        >  1:2758174/1‑68 (MQ=255)
                            ccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGcgcg                                        >  1:3695252/1‑68 (MQ=255)
                             cacaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTa                                                      <  1:1803765/53‑1 (MQ=255)
                              acaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTa                                                      <  1:2304325/52‑1 (MQ=255)
                              acaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCTCGTAGa                                    >  1:1400633/1‑70 (MQ=255)
                              acaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGa                                    >  1:4411152/1‑70 (MQ=255)
                              acaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGa                                    >  1:2646589/1‑70 (MQ=255)
                              acaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGa                                    >  1:410339/1‑70 (MQ=255)
                              acaTCTGTGGTCACGACGCAATCCGCAGGATTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGa                                    >  1:120901/1‑70 (MQ=255)
                                aTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGgcg                                          >  1:593844/1‑62 (MQ=255)
                                 tCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGc                                >  1:1718642/1‑71 (MQ=255)
                                  cTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaa                                                     >  1:1551325/1‑49 (MQ=255)
                                   tgtgGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGt                              >  1:4382518/1‑71 (MQ=255)
                                    gtgGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGt                                       <  1:1983157/61‑1 (MQ=255)
                                     tgGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAg                                     <  1:3442963/62‑1 (MQ=255)
                                     tgGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGc                                <  1:3597132/67‑1 (MQ=255)
                                     tgGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGt                              <  1:3201652/69‑1 (MQ=255)
                                      ggTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCAc                           >  1:1633293/1‑71 (MQ=255)
                                       gTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCAcc                          >  1:43353/1‑71 (MQ=255)
                                        tCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGt                                       <  1:1659611/57‑1 (MQ=255)
                                         cACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCgg                        >  1:3815356/1‑71 (MQ=255)
                                         cACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCgg                        >  1:2380935/1‑71 (MQ=255)
                                         cACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCgg                        >  1:1180766/1‑71 (MQ=255)
                                          acgacgCAATCCGCAGGTTTAGGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCgg                        <  1:136932/70‑1 (MQ=255)
                                          acgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaa                                                     >  1:947276/1‑41 (MQ=255)
                                          acgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAg                                 <  1:4260160/61‑1 (MQ=255)
                                          acgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAg                                 <  1:4045543/61‑1 (MQ=255)
                                          acgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCgg                        <  1:1964733/70‑1 (MQ=255)
                                          acgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCgg                        <  1:3406887/70‑1 (MQ=255)
                                          acgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCg                         >  1:3896996/1‑69 (MQ=255)
                                          acgaccCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCgt                        <  1:2867039/70‑2 (MQ=255)
                                           cgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGa                      <  1:3883797/71‑1 (MQ=255)
                                            gacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaacaac                                              >  1:3240704/1‑46 (MQ=255)
                                            gacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCTGGAt                     <  1:4321506/71‑1 (MQ=255)
                                            gacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGAt                     >  1:67465/1‑71 (MQ=255)
                                            gacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGAt                     >  1:1968924/1‑71 (MQ=255)
                                             acgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaacaac                                              <  1:4489085/45‑1 (MQ=255)
                                             acgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATg                    >  1:3366092/1‑71 (MQ=255)
                                              cgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATgg                   <  1:2918528/71‑1 (MQ=255)
                                               gCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATa                                  >  1:776731/1‑55 (MQ=255)
                                                cAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTc                 >  1:2863125/1‑71 (MQ=255)
                                                  aTCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTc                             <  1:4027958/57‑1 (MQ=255)
                                                  aTCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCg                >  1:2893585/1‑70 (MQ=255)
                                                  aTCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGt               <  1:1475097/71‑1 (MQ=255)
                                                  aTCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGt               <  1:3864165/71‑1 (MQ=255)
                                                  aTCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGt               <  1:4492450/71‑1 (MQ=255)
                                                  aTCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGt               <  1:3007071/71‑1 (MQ=255)
                                                   tCCGCATGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGt               >  1:592551/1‑70 (MQ=255)
                                                   tCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGt               >  1:3922004/1‑70 (MQ=255)
                                                   tCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTa              <  1:752322/71‑1 (MQ=255)
                                                    ccGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTc                             >  1:1783241/1‑55 (MQ=255)
                                                        aGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGc          >  1:81289/1‑70 (MQ=255)
                                                           tttACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAgg      <  1:4605734/71‑1 (MQ=255)
                                                            ttACGATCTGACAGTTGTTTTAACAACAACGTCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAgg      >  1:4379344/1‑70 (MQ=255)
                                                            ttACGATCTGACAGTTGTATTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAgc      >  1:3887415/1‑69 (MQ=255)
                                                             tACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAggca    >  1:1345004/1‑69 (MQ=255)
                                                             tACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAggca    >  1:4029675/1‑69 (MQ=255)
                                                               cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCTTAACGCCAGGCATg  >  1:2027726/1‑71 (MQ=255)
                                                               cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGcc         >  1:2410609/1‑64 (MQ=255)
                                                               cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATg  <  1:454582/71‑1 (MQ=255)
                                                               cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATg  <  1:4391037/71‑1 (MQ=255)
                                                                gATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGa                      >  1:425324/1‑50 (MQ=255)
                                                                gATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTaa             <  1:1850617/59‑1 (MQ=255)
                                                                    |                                                                 
TGCTGCGCAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCCGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGAATG  >  AP009048/3684970‑3685103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: