Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 3,684,885 T→C 100% pseudogene (174/582 nt)
pseudogene (1237/1645 nt)
ilvG ←
ilvG ←
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0090483,684,8850TC100.0% 92.2 / NA 28pseudogene (174/582 nt)
pseudogene (1237/1645 nt)
ilvG
ilvG
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/13);  total (15/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CATTCATCATGAAAGAGCCGTCACCGGAGATACAGACAACGGTATCGTTCGGTCGCGCGACTTGTGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTACCTAAACCGCTGGAGGTGATGAAATTTTCCG  >  AP009048/3684821‑3684952
                                                                |                                                                   
caTTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCTTTTGGTCGCGCGACTTgcgcgc                                                                 >  1:844571/1‑69 (MQ=255)
 aTTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCaa                                                              >  1:3198104/1‑71 (MQ=255)
   tcatcaTGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAc                                                             >  1:1155633/1‑70 (MQ=255)
    catcatGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAccg                                                           >  1:1169194/1‑71 (MQ=255)
        atGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAccgc                                                          >  1:2810192/1‑68 (MQ=255)
                gCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAc                                                             <  1:345486/57‑1 (MQ=255)
                 ccGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTgcgc                                                                   >  1:2526026/1‑50 (MQ=255)
                   gTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCa                                             >  1:2807933/1‑70 (MQ=255)
                   gTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCa                                             >  1:2767012/1‑70 (MQ=255)
                            gaTACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATgg                                   <  1:1477288/71‑1 (MQ=39)
                                cAGACGACGGTATCGTTTGGTCGCGCGACGTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGcc                               <  1:1743894/71‑1 (MQ=37)
                                  gacgacGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTa                             <  1:3049590/71‑1 (MQ=38)
                                  gacgacGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTa                             <  1:2187360/71‑1 (MQ=38)
                                    cgaCGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTaag                           >  1:1740978/3‑70 (MQ=38)
                                    cgaCGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTaa                            <  1:2470319/68‑1 (MQ=38)
                                     gaCGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAAcc                                              <  1:1986778/50‑1 (MQ=255)
                                              gTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTg                     >  1:4053355/1‑67 (MQ=37)
                                              gTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAg                  <  1:297134/70‑1 (MQ=38)
                                               tttggTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGt                <  1:2659561/68‑1 (MQ=38)
                                                 tggTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtga              >  1:1917852/2‑71 (MQ=38)
                                                   gTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCa                                      <  1:777658/45‑1 (MQ=255)
                                                    tCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtgatga           <  1:2599238/71‑1 (MQ=38)
                                                     cgcgcgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtgatga           >  1:813893/1‑70 (MQ=38)
                                                      gcgcgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtg               <  1:4197841/65‑1 (MQ=38)
                                                        gcgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAg                  >  1:1898285/1‑60 (MQ=37)
                                                          gACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAAtttt     >  1:4122375/1‑71 (MQ=38)
                                                           aCTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTgg                    <  1:4018472/55‑1 (MQ=37)
                                                            cTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAAt        >  1:1744106/1‑66 (MQ=38)
                                                             ttGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCg  >  1:230175/1‑71 (MQ=38)
                                                             ttGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGAGCCTAAGCCGCTGGAGGtg               >  1:3040201/1‑58 (MQ=37)
                                                                |                                                                   
CATTCATCATGAAAGAGCCGTCACCGGAGATACAGACAACGGTATCGTTCGGTCGCGCGACTTGTGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTACCTAAACCGCTGGAGGTGATGAAATTTTCCG  >  AP009048/3684821‑3684952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: