Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | AP009048 | 3,684,885 | T→C | 100% | pseudogene (174/582 nt) pseudogene (1237/1645 nt) |
ilvG ← ilvG ← |
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AP009048 | 3,684,885 | 0 | T | C | 100.0% | 92.2 / NA | 28 | pseudogene (174/582 nt) pseudogene (1237/1645 nt) | ilvG ilvG | ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/13); total (15/13) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CATTCATCATGAAAGAGCCGTCACCGGAGATACAGACAACGGTATCGTTCGGTCGCGCGACTTGTGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTACCTAAACCGCTGGAGGTGATGAAATTTTCCG > AP009048/3684821‑3684952 | caTTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCTTTTGGTCGCGCGACTTgcgcgc > 1:844571/1‑69 (MQ=255) aTTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCaa > 1:3198104/1‑71 (MQ=255) tcatcaTGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAc > 1:1155633/1‑70 (MQ=255) catcatGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAccg > 1:1169194/1‑71 (MQ=255) atGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAccgc > 1:2810192/1‑68 (MQ=255) gCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAc < 1:345486/57‑1 (MQ=255) ccGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTgcgc > 1:2526026/1‑50 (MQ=255) gTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCa > 1:2807933/1‑70 (MQ=255) gTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCa > 1:2767012/1‑70 (MQ=255) gaTACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATgg < 1:1477288/71‑1 (MQ=39) cAGACGACGGTATCGTTTGGTCGCGCGACGTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGcc < 1:1743894/71‑1 (MQ=37) gacgacGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTa < 1:3049590/71‑1 (MQ=38) gacgacGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTa < 1:2187360/71‑1 (MQ=38) cgaCGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTaag > 1:1740978/3‑70 (MQ=38) cgaCGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTaa < 1:2470319/68‑1 (MQ=38) gaCGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAAcc < 1:1986778/50‑1 (MQ=255) gTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTg > 1:4053355/1‑67 (MQ=37) gTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAg < 1:297134/70‑1 (MQ=38) tttggTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGt < 1:2659561/68‑1 (MQ=38) tggTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtga > 1:1917852/2‑71 (MQ=38) gTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCa < 1:777658/45‑1 (MQ=255) tCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtgatga < 1:2599238/71‑1 (MQ=38) cgcgcgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtgatga > 1:813893/1‑70 (MQ=38) gcgcgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtg < 1:4197841/65‑1 (MQ=38) gcgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAg > 1:1898285/1‑60 (MQ=37) gACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAAtttt > 1:4122375/1‑71 (MQ=38) aCTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTgg < 1:4018472/55‑1 (MQ=37) cTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAAt > 1:1744106/1‑66 (MQ=38) ttGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCg > 1:230175/1‑71 (MQ=38) ttGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGAGCCTAAGCCGCTGGAGGtg > 1:3040201/1‑58 (MQ=37) | CATTCATCATGAAAGAGCCGTCACCGGAGATACAGACAACGGTATCGTTCGGTCGCGCGACTTGTGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTACCTAAACCGCTGGAGGTGATGAAATTTTCCG > AP009048/3684821‑3684952 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |