Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 3,684,717 A→G 100% pseudogene (342/582 nt)
pseudogene (1405/1645 nt)
ilvG ←
ilvG ←
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0090483,684,7170AG100.0% 257.4 / NA 73pseudogene (342/582 nt)
pseudogene (1405/1645 nt)
ilvG
ilvG
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/41);  total (32/41)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCAGTAAGGGTGGTTTCGCTGTATCGTTCCTGAAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAA  >  AP009048/3684649‑3684782
                                                                    |                                                                 
ccGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaa                                                                 <  1:3361220/71‑1 (MQ=255)
  gAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaa                                                                >  1:1659292/1‑70 (MQ=255)
   aaGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaaa                                                              <  1:3476477/71‑1 (MQ=255)
    aGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAAc                                                             <  1:2759036/71‑1 (MQ=255)
    aGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAAc                                                             <  1:4346842/71‑1 (MQ=255)
     ggCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACa                                                            <  1:2769571/71‑1 (MQ=255)
     ggCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACa                                                            <  1:3125467/71‑1 (MQ=255)
      gcgcTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaaa                                                              <  1:1929771/68‑1 (MQ=255)
         cTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtg                                                        >  1:1995070/1‑71 (MQ=255)
          tGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaa                                                                 >  1:3805259/1‑61 (MQ=255)
          tGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgc                                                       >  1:2180314/1‑71 (MQ=255)
            gCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgctg                                                     >  1:2431920/1‑71 (MQ=255)
            gCTAACATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTtgc                                                       <  1:2325420/69‑1 (MQ=255)
               aaCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaa                                                                 <  1:4110467/56‑1 (MQ=255)
               aaCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACATTTGCTGCCa                                                  >  1:182588/1‑71 (MQ=255)
                aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCa                                                  <  1:1909768/70‑1 (MQ=255)
                aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCAt                                                 >  1:2973759/1‑71 (MQ=255)
                aCATGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCAt                                                 >  1:3785246/1‑71 (MQ=255)
                  aTGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaaa                                                               <  1:2189035/55‑1 (MQ=255)
                  aTGAGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTg                                               <  1:3644102/71‑1 (MQ=255)
                     aGGAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                                            <  1:32382/71‑1 (MQ=255)
                      ggAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGtt                                                         <  1:2643075/57‑1 (MQ=255)
                      ggAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                                            >  1:2055131/1‑70 (MQ=255)
                      ggAAATCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGa                                           <  1:1008042/71‑1 (MQ=255)
                        aaaTCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAAc                                         <  1:4073872/71‑1 (MQ=255)
                         aaTCGGGGTTATAGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAAc                                         >  1:1162679/1‑70 (MQ=255)
                          aTCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTTCCATTGTCGAACCa                                       <  1:2407477/71‑1 (MQ=255)
                           tCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGgaaa                                                                <  1:1958922/45‑1 (MQ=255)
                           tCGGGGTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCAt                                      >  1:2379354/1‑71 (MQ=255)
                             ggggTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCa                                                  >  1:475466/1‑57 (MQ=255)
                               ggTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCt                                  <  1:4040706/71‑1 (MQ=255)
                               ggTTATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCt                                  <  1:3825579/71‑1 (MQ=255)
                                 ttATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAAc                                                             <  1:3100464/42‑1 (MQ=255)
                                 ttATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAAc                                                             <  1:2133540/42‑1 (MQ=255)
                                 ttATCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTa                                 <  1:2949515/70‑1 (MQ=255)
                                   aTCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAg                                                           >  1:3078564/1‑42 (MQ=37)
                                   aTCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAAcc                              >  1:4110342/1‑71 (MQ=255)
                                   aTCGGTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAAc                               >  1:2422280/1‑70 (MQ=255)
                                      ggTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGtt                           <  1:4418650/70‑1 (MQ=255)
                                      ggTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGt                            <  1:1263346/69‑1 (MQ=255)
                                      ggTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGt                            <  1:130364/69‑1 (MQ=255)
                                      ggTAAGGGTGGTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCg                                            <  1:4401921/52‑1 (MQ=37)
                                       gTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAAc                                                             >  1:3700624/1‑36 (MQ=38)
                                       gTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGaa                                          >  1:2016151/1‑55 (MQ=255)
                                       gTAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTg                          >  1:1674555/1‑71 (MQ=255)
                                        tAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAg                                                           <  1:2074812/37‑1 (MQ=38)
                                        tAAGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTgg                         <  1:2444957/71‑1 (MQ=255)
                                         aaGGGTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGt                        >  1:883183/1‑71 (MQ=255)
                                           gggTGGTTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTa                                 <  1:4438184/60‑1 (MQ=255)
                                             gtggtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATc                    >  1:910935/1‑71 (MQ=255)
                                              tggtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATc                    >  1:4265559/1‑70 (MQ=255)
                                                gtTTCGCTATATCGTTCCTGGAAAACCAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGtt                       <  1:816572/65‑1 (MQ=255)
                                                gtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGa                  <  1:2943751/70‑1 (MQ=255)
                                                gtTTCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAg                 <  1:2304951/71‑1 (MQ=255)
                                                 tttCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATc                    <  1:2855850/67‑1 (MQ=255)
                                                  ttCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGt                >  1:4181730/1‑70 (MQ=255)
                                                   tagCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCa                                       >  1:1290813/3‑46 (MQ=38)
                                                   tCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGtt                       >  1:3059127/1‑62 (MQ=255)
                                                   tCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGt                        >  1:2403063/1‑61 (MQ=255)
                                                   tCGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGt                >  1:3815742/1‑69 (MQ=255)
                                                    cGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATc                    <  1:315591/64‑1 (MQ=255)
                                                    cGCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTaa              <  1:3849460/70‑1 (MQ=255)
                                                     gCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGtt                           <  1:4610294/56‑1 (MQ=255)
                                                     gCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAg                 <  1:562172/66‑1 (MQ=255)
                                                     gCTATATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGa            <  1:807844/71‑1 (MQ=255)
                                                     gCTATATCGTTCCTGGAAAAACAGTTGCTGCAATTGTCGAACCATCCCTAACCg                             >  1:2458472/1‑54 (MQ=38)
                                                      ctatATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGa            >  1:3632429/4‑70 (MQ=255)
                                                       tatATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTg                          >  1:2007920/3‑55 (MQ=255)
                                                        atATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGt                            <  1:732437/51‑1 (MQ=255)
                                                        atATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGt                            <  1:2065778/51‑1 (MQ=255)
                                                        atATCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTg                          >  1:93113/2‑54 (MQ=255)
                                                          aTCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGAtt       >  1:577400/1‑71 (MQ=255)
                                                          aTCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGAt        >  1:291365/1‑70 (MQ=255)
                                                           tCGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGAtt       <  1:3537335/70‑1 (MQ=255)
                                                            cGTTCCTGGAAAAACAGTTGCTGCCATTGTCGATCCATCCGTAACCg                             <  1:3051691/47‑1 (MQ=37)
                                                            cGTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGAtttt     >  1:1571440/1‑71 (MQ=255)
                                                             gTTCCTTGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTc    <  1:638037/71‑1 (MQ=255)
                                                             gTTCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTc    <  1:490753/71‑1 (MQ=255)
                                                               tCCTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCaa  >  1:4332076/1‑71 (MQ=255)
                                                                ccTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCaa  <  1:4521355/70‑1 (MQ=255)
                                                                acTGGAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCaa  <  1:1380695/69‑1 (MQ=255)
                                                                    |                                                                 
CCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCAGTAAGGGTGGTTTCGCTGTATCGTTCCTGAAAAAACAGTTGCTGCCATTGTCGAACCATCCCTAACCGTTGGTTATCGAGTAAGACGATTTTCAA  >  AP009048/3684649‑3684782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: