| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,332,615 | T→A | 11.3% | intergenic (‑25/+102) | yhbE ← / ← rpmA | conserved inner membrane protein/50S ribosomal subunit protein L27 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,332,615 | 0 | T | A | 11.3% | 188.8 / 15.7 | 71 | intergenic (‑25/+102) | yhbE/rpmA | conserved inner membrane protein/50S ribosomal subunit protein L27 |
| Reads supporting (aligned to +/- strand): ref base T (28/35); new base A (4/4); total (32/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01 | |||||||||||
CTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCG > AP009048/3332545‑3332684 | cTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCAttt < 1:3015660/71‑1 (MQ=255) tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTg < 1:1820230/69‑1 (MQ=255) tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGc < 1:1205504/70‑1 (MQ=255) gtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGa > 1:1501544/1‑71 (MQ=255) gAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAt > 1:1304204/1‑70 (MQ=255) aGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:489202/70‑1 (MQ=255) gcgcCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTaa < 1:4057308/71‑1 (MQ=255) cgcCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAAc < 1:3861070/71‑1 (MQ=255) cAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTc < 1:538794/71‑1 (MQ=255) cAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTc > 1:2765363/1‑71 (MQ=255) aaaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCg > 1:4433435/1‑71 (MQ=255) aaaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCg > 1:1768590/1‑71 (MQ=255) aaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCg > 1:4079586/1‑70 (MQ=255) aaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCa > 1:1452629/1‑71 (MQ=255) gAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCAcc < 1:1049844/71‑1 (MQ=255) gAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCAc > 1:3166635/1‑70 (MQ=255) ccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCAt > 1:1328817/1‑68 (MQ=255) ccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgcg < 1:4108968/71‑1 (MQ=255) caatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgcg > 1:4195488/1‑70 (MQ=255) atgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTaa > 1:4196276/1‑54 (MQ=255) atgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCa > 1:2436133/1‑64 (MQ=255) atgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAt < 1:2761564/71‑1 (MQ=255) atgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAt < 1:3495770/71‑1 (MQ=255) atgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAt > 1:282979/1‑71 (MQ=255) atgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCAt < 1:2835740/71‑1 (MQ=255) tgcctgccTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtt < 1:2680553/71‑1 (MQ=255) tgcctgccTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtt < 1:3056681/71‑1 (MQ=255) tgcctgccTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtt < 1:3075669/71‑1 (MQ=255) cctgcctgCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtttt < 1:3472586/71‑1 (MQ=255) cctgcctgCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtttt > 1:164298/1‑71 (MQ=255) cctgcctgCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtttt < 1:544296/71‑1 (MQ=255) ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCAc < 1:2762809/58‑1 (MQ=255) ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgcgc < 1:1243248/65‑1 (MQ=255) ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgcgc < 1:4387989/65‑1 (MQ=255) ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtttt > 1:4035572/1‑70 (MQ=255) ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAt < 1:2712001/67‑1 (MQ=255) ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCAttttt < 1:2105963/71‑1 (MQ=255) tgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTg > 1:1419660/1‑41 (MQ=255) cctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTAc < 1:2134198/70‑1 (MQ=255) ctgctgCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtttt > 1:535564/1‑66 (MQ=255) tgctgcTTCATACCGTATTCTCTGCAAAGCGCATTTGCGGAt < 1:3883794/42‑1 (MQ=255) tgctgcTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAt < 1:2501047/42‑1 (MQ=255) gctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAt < 1:1959243/61‑1 (MQ=255) gctgctTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGt > 1:2472270/1‑71 (MQ=255) ctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCgg < 1:1492044/38‑1 (MQ=255) ctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGtt < 1:4240906/71‑1 (MQ=255) ctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGtt < 1:3520368/71‑1 (MQ=255) ctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGtt < 1:3515199/71‑1 (MQ=255) gctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTAt > 1:2016403/1‑71 (MQ=255) ctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAt < 1:3551537/57‑1 (MQ=255) ctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTa > 1:2183502/1‑62 (MQ=255) tCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCAt > 1:1090082/1‑49 (MQ=255) tCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTAt > 1:1279057/1‑68 (MQ=255) cATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGaa > 1:154926/1‑71 (MQ=255) tACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAttttt < 1:1380479/56‑1 (MQ=255) aCCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCa > 1:1221017/1‑44 (MQ=255) ccGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGt > 1:2738651/1‑70 (MQ=255) ccGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTa < 1:4175929/71‑1 (MQ=255) cGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaa < 1:1995016/71‑1 (MQ=255) cGTATTCACTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTa > 1:1702118/1‑70 (MQ=255) tATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaaaa < 1:3192366/71‑1 (MQ=255) ttCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTAcc > 1:2932634/1‑52 (MQ=255) ttCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAg < 1:46863/71‑1 (MQ=255) cccTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGcc > 1:1663341/1‑71 (MQ=255) cTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaaaa > 1:2340012/1‑65 (MQ=255) gCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCg > 1:2019688/1‑70 (MQ=255) aaGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAAc < 1:1726543/71‑1 (MQ=255) aGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAAca < 1:551219/71‑2 (MQ=255) gcgcATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATc > 1:3632204/1‑48 (MQ=255) gcgcATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATc > 1:3127551/1‑48 (MQ=255) gcgcATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACgt < 1:1029988/71‑1 (MQ=255) aTTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTg < 1:289853/71‑1 (MQ=255) tttGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGcccc < 1:1098720/59‑1 (MQ=255) ttGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAg > 1:4553732/1‑54 (MQ=255) tGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCg < 1:482126/70‑1 (MQ=255) | CTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCG > AP009048/3332545‑3332684 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |