New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP009048 3277760 =98 (1.960)3 (0.070) 3/94 NT 3.1% coding (761/810 nt) agaR DNA‑binding transcriptional dual regulator
?AP009048 3277781 = 98 (2.130)coding (740/810 nt) agaR DNA‑binding transcriptional dual regulator
Rejected: Frequency below/above cutoff threshold.

AGTTTACATAAGATCATTGATACTCAACGTATCGACATGATCATTGTTGATGAAGGCATTCCTGCGGATAGCCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AP009048/3277833‑3277760
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcctTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTA  >  AP009048/3277781‑3277849
                                                                                                                                               
AGTTTACATAAGATCATTGATACTCAACGTATCGACATGATCATTGTTGATGAAGGCATTCCTGCGGATAG                                                                          <  1:3226732/71‑1
 GTTTACATAAGATCCTTGATACTCAACGTATCGACATGATCATTGTTGATGAAGGCATTCCTGCGGATAGC                                                                         <  1:2260623/71‑1
 GTTTACATAAGATCATTGATACTCAACGTATCGACATGATCATTGTTGATGAAGGCATTCCTGCGGATAGC                                                                         <  1:1505103/71‑1
 GTTTACATAAGATCATTGATACTCAACGTATCGACATGATCATTGTTGATGAAGGCATTCCTGCGGATAG                                                                          >  1:1587327/1‑70
   TTACATAAGATCATTGATACTCAACGTATCGACATGATCATTGTTGATGAAGGCATTCCTGCGGATAGCCT                                                                       >  1:2156086/1‑71
              CATTGATACTCAACGTATCGACATGATCATTGTTGATGAAGGCATTCCTGCGGATAGC                                                                         <  1:4112851/58‑1
                              ATCGACATGATCATTGTTGATGAAGGCATTCCTGCGGATAGCC                                                                        >  1:4246399/1‑43
                                  ACATGATCATTGTTGATGAAGGCATTCCTGCGGATAGCC                                                                        <  1:2024364/39‑1
                                    ATGATCATTGTTGATGAAGGCATTCCTGCGGATAGCCT                                                                       >  1:1844630/1‑38
                                                GATGAAGGCATTCCTGCGGATAGCCTTCATCAACAATGATCATGTCGATACGTTGAGAATCAATGATCTTA                          >  1:2404946/1‑71
                                                         ATTCCTGCGGATAGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATC                             <  1:3850947/59‑1
                                                           TCCTGCGGATAGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCA                                   >  1:81864/1‑51
                                                                      GCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACT    >  1:3592452/1‑71
                                                                      GCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAAC     >  1:3670666/1‑70
                                                                       CCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTT   <  1:3675916/71‑1
                                                                       CCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTT   <  1:517186/71‑1
                                                                       CCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTT   <  1:4097331/71‑1
                                                                       CCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACT    >  1:9450/1‑70
                                                                       CCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACT    <  1:3860991/70‑1
                                                                       CCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTT                           <  1:3699085/47‑1
                                                                       CCTTCATCAACAATGATCATATAGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACT    <  1:1571902/70‑1
                                                                        CTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTA  <  1:4360280/71‑1
                                                                         TTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCT                            <  1:4046569/44‑1
                                                                                                                                               
AGTTTACATAAGATCATTGATACTCAACGTATCGACATGATCATTGTTGATGAAGGCATTCCTGCGGATAGCCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AP009048/3277833‑3277760
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcctTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTA  >  AP009048/3277781‑3277849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.