Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC AP009048 654,214 Δ1,199 bp 100% [dcuC][dcuC] [dcuC], insH, [dcuC]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ AP009048 654214–655411 655412 2–1199 37 [0] [0] 38 [dcuC]–[dcuC] [dcuC],insH,[dcuC]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP009048 = 6542130 (0.000)27 (0.600) 22/92 NT 100% coding (70/477 nt) dcuC anaerobic C4‑dicarboxylate transport
?AP009048 655413 = 0 (0.000)coding (978/1092 nt) dcuC anaerobic C4‑dicarboxylate transport

ATCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP009048/654140‑654213
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGC  >  AP009048/655413‑655480
                                                                                                                                              
ATCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCT                                                                         <  1:4198486/71‑1
       TGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCC                                                                  >  1:4362365/1‑71
       TGATACTCGCCGACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCT                                                                 <  1:1663362/71‑1
         ATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCT                                                                 <  1:1034257/70‑1
         ATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCT                                                                 <  1:735774/70‑1
             TCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGC                                                             >  1:1897106/1‑70
             TCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGC                                                             >  1:4403718/1‑70
                  GAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATAC                                                       >  1:33430/1‑71
                   AACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATAC                                                       >  1:2251801/1‑70
                      CAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCC                                                     >  1:535084/1‑69
                      CAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATAACCCA                                                   <  1:3723967/71‑1
                          CGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGG                                               >  1:4313540/1‑71
                              GTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAAC                                           <  1:3735311/71‑1
                              GTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAAC                                           <  1:4027955/71‑1
                               TAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCT                                                                         <  1:4012883/40‑1
                                AGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGA                                                               <  1:1226280/49‑1
                                   GATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCA                                      <  1:1788554/71‑1
                                    ATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAG                                                  <  1:1997302/58‑1
                                     TAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAG                                     <  1:3440748/70‑1
                                       GAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCAT                                  >  1:2835469/1‑71
                                       GAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCATGAGCGAATA                                                        >  1:2742746/1‑49
                                         AATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGC                                       >  1:1722665/1‑64
                                             AGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCA                             >  1:2204787/1‑70
                                               ACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGT                          <  1:4553243/71‑1
                                                CTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGC                                              <  1:4539066/50‑1
                                                 TTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGT                          >  1:321825/1‑69
                                                  TTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGC                                       >  1:2685951/1‑55
                                                   TGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAG                       >  1:2965978/1‑70
                                                     AATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCA                     <  1:2450420/70‑1
                                                         AAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCGGCATCACCACGTTAGCAAACGC                <  1:1543212/71‑1
                                                         AAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGC                <  1:1570314/71‑1
                                                         AAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGC                <  1:2522769/71‑1
                                                         AAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACG                 >  1:2832126/1‑70
                                                                TGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGT                          <  1:435985/54‑1
                                                                TGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGT                          <  1:441575/54‑1
                                                                       AAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGC  >  1:2786831/1‑71
                                                                       AAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCG   >  1:333620/1‑70
                                                                        AGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGC  >  1:101440/1‑70
                                                                         GCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTA                        >  1:2191627/1‑47
                                                                                                                                              
ATCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP009048/654140‑654213
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGC  >  AP009048/655413‑655480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.