| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,185,038 | A→G | 8.4% | I335V (ATT→GTT) ‡ | yeaG → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,185,038 | 0 | A | G | 8.4% | 387.5 / 18.1 | 131 | I335V (ATT→GTT) ‡ | yeaG | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base A (50/70); new base G (9/2); total (59/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 133 total aligned reads displayed.
CCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCA > Exported/1184968‑1185100 | ccGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCa > 1:280797/1‑71 (MQ=255) taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg < 1:4966717/71‑1 (MQ=255) taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg < 1:2328440/71‑1 (MQ=255) taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg < 1:1500764/71‑1 (MQ=255) taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg < 1:1346692/71‑1 (MQ=255) taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg < 1:1347661/71‑1 (MQ=255) aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:106081/1‑69 (MQ=255) aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:308727/1‑69 (MQ=255) aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:1417489/1‑69 (MQ=255) aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGACGTATTGCTTGCGCATTTCCg > 1:4213012/1‑69 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTcag > 1:3904249/1‑66 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTc > 1:4035432/1‑66 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:3690936/1‑68 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:1907883/1‑68 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:4716675/1‑68 (MQ=255) cAAAAACAAAGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:4307666/1‑68 (MQ=255) aaaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:2582564/1‑69 (MQ=255) aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCa > 1:3467636/1‑60 (MQ=255) aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:2166856/1‑68 (MQ=255) aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:1947465/1‑68 (MQ=255) aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:4081742/1‑68 (MQ=255) aaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:1081638/1‑67 (MQ=255) caacGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATc > 1:2834938/1‑70 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:2730294/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:408721/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:1061672/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:3124410/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:3615779/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:4423473/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:1582473/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:658818/1‑68 (MQ=255) acGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:289350/71‑1 (MQ=255) gAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAAt < 1:2536838/71‑1 (MQ=255) aGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCt < 1:4073208/71‑1 (MQ=255) ttCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGAtga < 1:4543288/56‑1 (MQ=255) ccTCGATCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGAtga < 1:1753935/54‑1 (MQ=255) cTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga < 1:3221779/70‑1 (MQ=255) cTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga < 1:2976790/70‑1 (MQ=255) cTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGaa < 1:736434/71‑1 (MQ=255) tCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga < 1:2323156/69‑1 (MQ=255) tCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga < 1:3825626/69‑1 (MQ=255) tCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga < 1:2563254/69‑1 (MQ=255) tCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGaa < 1:2424165/70‑1 (MQ=255) gtgtTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCtt < 1:1779497/71‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:1065409/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:4740196/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:337610/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:3965795/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:1884815/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:709103/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:2447266/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:1879344/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAAt < 1:4510640/71‑1 (MQ=255) ttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:4195978/49‑1 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGc > 1:321991/1‑64 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGc > 1:4222585/1‑64 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGc > 1:2727891/1‑64 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGc > 1:1378579/1‑64 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGc > 1:127299/1‑64 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATca > 1:874782/1‑71 (MQ=255) aCATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:4687909/1‑38 (MQ=255) aCATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:2762159/1‑38 (MQ=255) aCATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGa > 1:3987160/1‑37 (MQ=255) cATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAACCTACGAGAAATTGc > 1:4261839/1‑62 (MQ=255) gTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGGAATTGCTTAATCACAGTg < 1:2356672/70‑1 (MQ=255) gTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATca < 1:5196985/65‑1 (MQ=255) gTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATca < 1:2437766/65‑1 (MQ=255) gTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTg < 1:1840053/70‑1 (MQ=255) gTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTg < 1:3073442/70‑1 (MQ=255) gTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGa < 1:3037715/71‑1 (MQ=255) ttaaGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGaa < 1:4327914/69‑1 (MQ=255) tGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGaa < 1:529655/71‑1 (MQ=255) tGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGaa < 1:430561/71‑1 (MQ=255) aGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCtt < 1:1816405/56‑1 (MQ=255) aGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCtt < 1:174743/56‑1 (MQ=255) aGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCtt < 1:2472414/56‑1 (MQ=255) aGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCtt < 1:5008081/56‑1 (MQ=255) aGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTg < 1:4285152/71‑1 (MQ=255) aGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTg < 1:1040723/71‑1 (MQ=255) aGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTg < 1:5046679/71‑1 (MQ=255) ggTGCCGTATTGCTTGCGCATTTCCGAAGAGATCa < 1:4930530/35‑1 (MQ=255) ggTGCCGTATTGCTTGCGCATTTCCGAAGAGATCa < 1:3574465/35‑1 (MQ=255) ggTGCCGTATTGCTTGCGCATTTCCGAAGAGATCa < 1:4293411/35‑1 (MQ=255) ggTGCCGTATTGCTTGCGCATTTCCGAAGAGATCa < 1:2553684/35‑1 (MQ=255) ggTGCCGTATTGCTTGCGCATTTCCGAAGAGATCa < 1:341536/35‑1 (MQ=255) tGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACt < 1:1807940/71‑1 (MQ=255) ttgcttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGccc > 1:1520917/1‑71 (MQ=255) ttgcttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGccc > 1:2527608/1‑71 (MQ=255) gcttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGaa > 1:5121104/1‑56 (MQ=255) gcttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGaa > 1:1649934/1‑56 (MQ=255) gcttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGaa > 1:625134/1‑56 (MQ=255) gcttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGaa > 1:2013200/1‑56 (MQ=255) gcttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGaa > 1:2820106/1‑56 (MQ=255) gcttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCa > 1:3677099/1‑71 (MQ=255) gcttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCa > 1:4901218/1‑71 (MQ=255) ttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTg < 1:5069451/52‑1 (MQ=255) tgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAAtt < 1:3288585/55‑1 (MQ=255) tgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAAtt < 1:114552/55‑1 (MQ=255) tgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGAGAAtt < 1:148272/55‑1 (MQ=255) gcATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATca < 1:850367/43‑1 (MQ=255) | CCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCA > Exported/1184968‑1185100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |