Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,185,007 T→C 13.3% V324V (GTT→GTC) ‡ yeaG → conserved hypothetical protein with nucleoside triphosphate hydrolase domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,185,0070TC13.3% 255.2 / 26.5 98V324V (GTT→GTC) ‡yeaGconserved hypothetical protein with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base T (28/57);  new base C (11/2);  total (39/59)
Fisher's exact test for biased strand distribution p-value = 5.78e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 111 total aligned reads displayed.

ACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAAT  >  Exported/1184938‑1185076
                                                                     |                                                                     
acacTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGttt                                                                      <  1:4643252/71‑1 (MQ=255)
      gAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCg                                                                >  1:1649587/1‑71 (MQ=255)
         cGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:2610481/71‑1 (MQ=255)
         cGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:218075/71‑1 (MQ=255)
         cGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:5153118/71‑1 (MQ=255)
         cGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:3669232/71‑1 (MQ=255)
         cGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:899725/71‑1 (MQ=255)
          gAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGaa                                                            <  1:4863717/71‑1 (MQ=255)
                   tGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGt                                                    >  1:841094/1‑70 (MQ=255)
                    gggTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGaa                                                            <  1:2938971/61‑1 (MQ=255)
                     tgTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAtt                                                 <  1:3752969/70‑1 (MQ=255)
                     ggTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAtt                                                 <  1:1239636/71‑1 (MQ=255)
                     ggTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAtt                                                 <  1:1176660/71‑1 (MQ=255)
                          cTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAttgcttg                                            <  1:3346598/71‑1 (MQ=255)
                          cTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAttgcttg                                            <  1:3491505/71‑1 (MQ=255)
                          cTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAttgcttg                                            <  1:3220125/71‑1 (MQ=255)
                          cTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAttgcttg                                            <  1:3882260/71‑1 (MQ=255)
                          cTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAttgcttg                                            <  1:4523425/71‑1 (MQ=255)
                           tttCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:5108633/53‑1 (MQ=255)
                           tttCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:2367878/53‑1 (MQ=255)
                              ccGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCa                                        >  1:280797/1‑71 (MQ=255)
                                gTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:2074062/48‑1 (MQ=255)
                                gTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:3250381/48‑1 (MQ=255)
                                gTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:637328/48‑1 (MQ=255)
                                gTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:2545427/48‑1 (MQ=255)
                                gTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:4089734/48‑1 (MQ=255)
                                gTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:4829017/48‑1 (MQ=255)
                                gTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:2128243/48‑1 (MQ=255)
                                gTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:4964384/48‑1 (MQ=255)
                                 taataaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:4254376/47‑1 (MQ=255)
                                 taataaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa                                                             <  1:4002593/47‑1 (MQ=255)
                                    taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                  <  1:1347661/71‑1 (MQ=255)
                                    taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                  <  1:1346692/71‑1 (MQ=255)
                                    taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                  <  1:2328440/71‑1 (MQ=255)
                                    taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                  <  1:2230309/71‑1 (MQ=255)
                                    taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                  <  1:4328090/71‑1 (MQ=255)
                                     aaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTgcgc                                         <  1:1580750/63‑1 (MQ=255)
                                     aaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTgcgc                                         <  1:4457892/63‑1 (MQ=255)
                                     aaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTgcgc                                         <  1:3312983/63‑1 (MQ=255)
                                     aaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTgcgc                                         <  1:3078019/63‑1 (MQ=255)
                                      aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                  >  1:1417489/1‑69 (MQ=255)
                                      aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                  >  1:2724458/1‑69 (MQ=255)
                                      aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                  >  1:1273432/1‑69 (MQ=255)
                                      aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                  >  1:106081/1‑69 (MQ=255)
                                      aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                  >  1:308727/1‑69 (MQ=255)
                                      aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGACGTATTGCTTGCGCATTTCCg                                  >  1:4213012/1‑69 (MQ=255)
                                       cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGt                                                    >  1:3336316/1‑50 (MQ=255)
                                       cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTcag                                  >  1:3904249/1‑66 (MQ=255)
                                       cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTc                                    >  1:4035432/1‑66 (MQ=255)
                                       cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                  >  1:2544656/1‑68 (MQ=255)
                                       cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                  >  1:3690936/1‑68 (MQ=255)
                                       cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                  >  1:1907883/1‑68 (MQ=255)
                                       cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                  >  1:4716675/1‑68 (MQ=255)
                                       cAAAAACAAAGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg                                  >  1:4307666/1‑68 (MQ=255)
                                        aaaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa                                >  1:4189999/1‑69 (MQ=255)
                                        aaaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa                                >  1:2582564/1‑69 (MQ=255)
                                         aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa                                >  1:4081742/1‑68 (MQ=255)
                                         aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa                                >  1:3909642/1‑68 (MQ=255)
                                         aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa                                >  1:2166856/1‑68 (MQ=255)
                                         aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa                                >  1:4838604/1‑68 (MQ=255)
                                          aaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTg                                                              <  1:3783730/37‑1 (MQ=255)
                                          aaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTg                                                              <  1:1701134/37‑1 (MQ=255)
                                          aaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTg                                                              <  1:2529728/37‑1 (MQ=255)
                                          aaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa                                >  1:1081638/1‑67 (MQ=255)
                                             caacGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATc                          >  1:2834938/1‑70 (MQ=255)
                                               acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTAt                                                  >  1:3568936/1‑44 (MQ=16)
                                               acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTgcg                                          >  1:3466443/1‑52 (MQ=19)
                                               acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc                          >  1:4423473/1‑68 (MQ=255)
                                               acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc                          >  1:1582473/1‑68 (MQ=255)
                                               acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc                          >  1:531556/1‑68 (MQ=255)
                                               acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc                          >  1:658818/1‑68 (MQ=255)
                                               acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc                          >  1:1061672/1‑68 (MQ=255)
                                               acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc                          >  1:3124410/1‑68 (MQ=255)
                                               acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc                          >  1:3615779/1‑68 (MQ=255)
                                               acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc                          >  1:2730294/1‑68 (MQ=255)
                                               acGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa                       <  1:289350/71‑1 (MQ=255)
                                                 gAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAAt                     <  1:2536838/71‑1 (MQ=255)
                                                   aGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCt                   <  1:4073208/71‑1 (MQ=255)
                                                       ttCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGAtga                              <  1:4543288/56‑1 (MQ=255)
                                                         ccTCGATCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGAtga                              <  1:1753935/54‑1 (MQ=255)
                                                          cTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga             <  1:3221779/70‑1 (MQ=255)
                                                          cTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga             <  1:2976790/70‑1 (MQ=255)
                                                          cTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga             <  1:4069448/70‑1 (MQ=255)
                                                          cTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGaa            <  1:736434/71‑1 (MQ=255)
                                                           tCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga             <  1:3825626/69‑1 (MQ=255)
                                                           tCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga             <  1:2563254/69‑1 (MQ=255)
                                                           tCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga             <  1:2323156/69‑1 (MQ=255)
                                                           tCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGaa            <  1:2424165/70‑1 (MQ=255)
                                                            cGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGaa            <  1:3375686/69‑1 (MQ=255)
                                                                 gtgtTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCtt     <  1:1779497/71‑1 (MQ=255)
                                                                 gtgtTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCtt     <  1:3686387/71‑1 (MQ=255)
                                                                    tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa                       <  1:337610/50‑1 (MQ=255)
                                                                    tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa                       <  1:1065409/50‑1 (MQ=255)
                                                                    tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa                       <  1:709103/50‑1 (MQ=255)
                                                                    tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa                       <  1:3965795/50‑1 (MQ=255)
                                                                    tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa                       <  1:1879344/50‑1 (MQ=255)
                                                                    tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa                       <  1:4740196/50‑1 (MQ=255)
                                                                    tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa                       <  1:1884815/50‑1 (MQ=255)
                                                                    tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAAt  >  1:3043214/1‑71 (MQ=255)
                                                                     ttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa                       <  1:4195978/49‑1 (MQ=255)
                                                                     |                                                                     
ACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAAT  >  Exported/1184938‑1185076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: