BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,080,862	A→G	11.9%	N220N (AAT→AAC)	ydhV ←	predicted oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,080,862	0	A	G	11.9%	340.1 / 29.9	126	N220N (AAT→AAC)	ydhV	predicted oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (38/73); new base G (10/5); total (48/78)
Fisher's exact test for biased strand distribution p-value = 2.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 147 total aligned reads displayed.

CCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTT  >  Exported/1080792‑1080930

cccACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCa                                                                      >  1:2496402/1‑71 (MQ=255)

cccACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCa                                                                      >  1:5061062/1‑71 (MQ=255)

cccACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCa                                                                      >  1:4374090/1‑71 (MQ=255)

 ccACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCAt                                                                     >  1:2980655/1‑71 (MQ=255)

 ccACGAGTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCAt                                                                     >  1:4996416/1‑71 (MQ=255)

  cACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCAtt                                                                    <  1:1302593/71‑1 (MQ=255)

  cACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCAtt                                                                    <  1:2403763/71‑1 (MQ=255)

  cACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCAtt                                                                    <  1:4518954/71‑1 (MQ=255)

  cACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCAtt                                                                    >  1:3101852/1‑71 (MQ=255)

  cACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCAtt                                                                    >  1:2824053/1‑71 (MQ=255)

    cGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCa                                                                      <  1:5199041/67‑1 (MQ=255)

         gtgGCGTACTTGGCACGACATTGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCAtt                                                                    <  1:4233746/64‑1 (MQ=255)

         gtgGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCAtt                                                                    <  1:2381966/64‑1 (MQ=255)

         gtgGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCAtt                                                                    <  1:616230/64‑1 (MQ=255)

         gtgGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCAtt                                                                    <  1:2585107/64‑1 (MQ=255)

         gtgGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCAtt                                                                    <  1:3690571/64‑1 (MQ=255)

         gtgGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCAtt                                                                    <  1:462075/64‑1 (MQ=255)

         gtgGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCAtt                                                                    <  1:3105380/64‑1 (MQ=255)

          tgGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGc                                                             <  1:2759399/70‑1 (MQ=255)

           ggCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTga                                                                  >  1:5087803/1‑64 (MQ=255)

            gCGTACTTGGCACGAGATGGTTGTTATTCGCACCAATAACTTCAGTCATCATGTAATCATTga                                                                  <  1:3586274/63‑1 (MQ=255)

            gCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCtt                                                           >  1:365764/1‑70 (MQ=255)

            gCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCtt                                                           >  1:4218165/1‑70 (MQ=255)

            gCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCtt                                                           >  1:4390438/1‑70 (MQ=255)

            gCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCtt                                                           >  1:2957173/1‑70 (MQ=255)

            gCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCtt                                                           >  1:4176171/1‑70 (MQ=255)

             cGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGc                                                             <  1:1696250/67‑1 (MQ=255)

               tACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                                                       >  1:3486645/1‑71 (MQ=255)

               tACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                                                       >  1:603142/1‑71 (MQ=255)

               tACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                                                       >  1:722342/1‑71 (MQ=255)

               tACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                                                       >  1:1483688/1‑71 (MQ=255)

               tACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                                                       >  1:1768483/1‑71 (MQ=255)

                       aCGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACg                                               <  1:3335017/71‑1 (MQ=255)

                       aCGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACg                                               <  1:3329183/71‑1 (MQ=255)

                       aCGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACg                                               <  1:3208646/71‑1 (MQ=255)

                       aCGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACg                                               <  1:860873/71‑1 (MQ=255)

                       aCGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACg                                               <  1:1391160/71‑1 (MQ=255)

                       aCGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACg                                               <  1:1927140/71‑1 (MQ=255)

                       aCGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACg                                               <  1:1545241/71‑1 (MQ=255)

                       aCGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACg                                               <  1:4795164/71‑1 (MQ=255)

                        cGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGa                                              <  1:4897321/71‑1 (MQ=255)

                             tGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGc                                         <  1:1570454/71‑1 (MQ=255)

                              ggTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCa                                                                      >  1:4831545/1‑41 (MQ=255)

                              ggTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCa                                                                      >  1:2867851/1‑41 (MQ=255)

                              ggTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCa                                                                      >  1:1819514/1‑41 (MQ=255)

                              ggTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCa                                                                      >  1:1832114/1‑41 (MQ=255)

                              ggTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCa                                                                      >  1:2684462/1‑41 (MQ=255)

                              ggTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCa                                                                      >  1:1995606/1‑41 (MQ=255)

                              ggTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCa                                                                      >  1:4213480/1‑41 (MQ=255)

                              ggTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCa                                                                      >  1:2491250/1‑41 (MQ=255)

                                  gttATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATg                                     <  1:5042435/70‑1 (MQ=255)

                                  gttATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATg                                     <  1:3420579/70‑1 (MQ=255)

                                  gttATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGt                                    >  1:5036359/1‑71 (MQ=255)

                                   ttATTCGCACCAATAAGTTCAGTCATCATGTAATCATTga                                                                  <  1:3557915/40‑1 (MQ=255)

                                   ttATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt                                   <  1:4634596/71‑1 (MQ=255)

                                   ttATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt                                   <  1:4736456/71‑1 (MQ=255)

                                   ttATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt                                   <  1:148201/71‑1 (MQ=255)

                                   ttATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt                                   <  1:2298356/71‑1 (MQ=255)

                                   ttATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt                                   <  1:885716/71‑1 (MQ=255)

                                   ttATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt                                   <  1:866093/71‑1 (MQ=255)

                                            ccAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGa                                 <  1:266918/64‑1 (MQ=255)

                                            ccAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCttt                          <  1:1398609/71‑1 (MQ=255)

                                            ccAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCttt                          <  1:4918728/71‑1 (MQ=255)

                                            ccAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCttt                          <  1:1664606/71‑1 (MQ=255)

                                            ccAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCttt                          <  1:4032031/71‑1 (MQ=255)

                                            ccAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCttt                          <  1:2185392/71‑1 (MQ=255)

                                             cAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCttt                          <  1:1671244/70‑1 (MQ=255)

                                                   gTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCt                   <  1:277761/71‑1 (MQ=255)

                                                        gTCATCATGTAATCGTTGAGACGCTTCATCTCCTGACGATc                                            <  1:2954840/41‑1 (MQ=255)

                                                        gTCATCATGTAATCGTTGAGACGCTTCATCTCCTGACGATc                                            <  1:2357979/41‑1 (MQ=255)

                                                        gTCATCATGTAATCGTTGAGACGCTTCATCTCCTGACGATc                                            <  1:2397531/41‑1 (MQ=255)

                                                          catcatGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCg            >  1:1317554/1‑71 (MQ=255)

                                                           atcatGTAATCGTTGAGACGCTTCATCTCCTGACGATCgg                                          >  1:3763275/1‑40 (MQ=255)

                                                           atcatGTAATCGTTGAGACGCTTCATCTCCTGACGATCGGCaa                                       >  1:802952/1‑43 (MQ=255)

                                                           atcatGTAATCGTTGAGACGCTTCATCTCCTGACGATCGGCaa                                       >  1:4969986/1‑43 (MQ=255)

                                                           atcatGTAATCGTTGAGACGCTTCATCTCCTGACGATCGGCaa                                       >  1:962713/1‑43 (MQ=255)

                                                           atcatGTAATCGTTGAGACGCTTCATCTCCTGACGATCGGCaa                                       >  1:3936311/1‑43 (MQ=255)

                                                           atcatGTAATCGTTGAGACGCTTCATCTCCTGACGATCGGCaa                                       >  1:3406612/1‑43 (MQ=255)

                                                           atcatGTAATCGTTGAGACGCTTCATCTCCTGACGATCGGCaa                                       >  1:3366673/1‑43 (MQ=255)

                                                           atcatGTAATCGTTGAGACGCTTCATCTCCTGACGATCGGCaa                                       >  1:1927780/1‑43 (MQ=255)

                                                             catGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATg                                     <  1:2128595/43‑1 (MQ=255)

                                                              atGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCaa         <  1:387777/70‑1 (MQ=255)

                                                              atGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCaa         <  1:620851/70‑1 (MQ=255)

                                                              atGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAAt        >  1:1316232/1‑71 (MQ=255)

                                                               tGTAATCATTGAGACGCTTCATCTCCTGACTATCGGCAATGTTGACCCCTTTCGTCCCTTCATCCGCaa         <  1:2143853/69‑1 (MQ=255)

                                                               tGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATc       >  1:3895208/1‑71 (MQ=255)

                                                               tGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATc       >  1:2991517/1‑71 (MQ=255)

                                                               tGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATc       >  1:4454916/1‑71 (MQ=255)

                                                                 tAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt                                   <  1:3324654/41‑1 (MQ=255)

                                                                  aaTCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCt    >  1:5150714/1‑71 (MQ=255)

                                                                  aaTCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCt    >  1:2252239/1‑71 (MQ=255)

                                                                   aTCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCttt                          >  1:1919930/1‑48 (MQ=255)

                                                                    tCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCttt  <  1:5114812/71‑1 (MQ=255)

                                                                    tCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCttt  <  1:2575082/71‑1 (MQ=255)

                                                                    tCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCttt  <  1:1976933/71‑1 (MQ=255)

                                                                     cATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCttt  >  1:2215238/1‑70 (MQ=255)

                                                                      aTTGAGCCGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCttt  >  1:3168883/1‑69 (MQ=255)

                                                                      aTTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCttt  >  1:2571614/1‑69 (MQ=255)

                                                                      aTTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCttt  >  1:628724/1‑69 (MQ=255)

                                                                      aTTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCttt  >  1:4802150/1‑69 (MQ=255)

CCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTTGACCCCTTTCGTCCCTTCAACCGCAATCGCTTT  >  Exported/1080792‑1080930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`