| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 42,526 | C→T | 15.9% | G223G (GGC→GGT) | fixC → | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 42,526 | 0 | C | T | 15.9% | 151.5 / 19.3 | 63 | G223G (GGC→GGT) | fixC | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base C (20/33); new base T (4/6); total (24/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01 | |||||||||||
AGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCAT > Exported/42459‑42592 | aGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGggcggcgg < 1:4041808/70‑1 (MQ=255) ttGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCtt < 1:3062839/71‑1 (MQ=255) ttGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCtt < 1:3056932/71‑1 (MQ=255) ttGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCtt < 1:4986736/71‑1 (MQ=255) ttGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCtt < 1:4001868/71‑1 (MQ=255) aGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAAt > 1:2651428/1‑71 (MQ=255) aGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAAt > 1:5009783/1‑71 (MQ=255) aGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAAt > 1:2995602/1‑71 (MQ=255) aGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAAt > 1:4243258/1‑71 (MQ=255) aGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAAt > 1:5075967/1‑71 (MQ=255) gggCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGaa < 1:2225105/71‑1 (MQ=255) gggCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGaa < 1:1712612/71‑1 (MQ=255) cgcggcTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGTGGCTTCCTTTATACCAATGaaa < 1:4350033/70‑1 (MQ=255) cgcggcTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGTGGCTTCCTTTATACCAATGaaa < 1:4805577/70‑1 (MQ=255) cgcggcTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGTGGCTTCCTTTATACCAATGaaa < 1:1352595/70‑1 (MQ=255) cgcggcTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGTGGCTTCCTTTATACCAATGaaa < 1:487039/70‑1 (MQ=255) gcggcTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGTGGCTTCCTTTATACCAATGaaa < 1:4643629/70‑1 (MQ=255) gcTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCtt < 1:2792505/54‑1 (MQ=255) gcTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCtt < 1:435230/54‑1 (MQ=255) gcTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCtt < 1:1258780/54‑1 (MQ=255) gcTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCtt < 1:3258249/54‑1 (MQ=255) gcTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCtt < 1:3420484/54‑1 (MQ=255) gcTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAaca < 1:5068050/70‑1 (MQ=255) ttGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTta > 1:3640298/1‑54 (MQ=255) tGCCTGTTTGCGGGAGCACCCACCGATGGCCTGATGGGCGGTGGCTTCCTTTATACCAATGaaa < 1:4039687/64‑1 (MQ=39) tttGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCg < 1:4455285/69‑1 (MQ=255) tttGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCg < 1:3214205/69‑1 (MQ=255) tttGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCt > 1:2014676/1‑71 (MQ=255) tGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTgg > 1:3223722/1‑71 (MQ=255) tGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTgg > 1:4588741/1‑71 (MQ=255) tGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTgg > 1:1043880/1‑71 (MQ=255) tGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTgg > 1:3480169/1‑71 (MQ=255) tGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTgg > 1:2660637/1‑71 (MQ=255) gCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTggg > 1:1609318/1‑71 (MQ=255) gCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTggg > 1:2533731/1‑71 (MQ=255) ggATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTc < 1:774576/62‑1 (MQ=255) ggATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTc < 1:3041925/62‑1 (MQ=255) ggATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTc < 1:601522/62‑1 (MQ=255) ggATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCt < 1:348594/71‑1 (MQ=255) cGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCt < 1:1969540/60‑1 (MQ=255) gATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAac > 1:3677316/1‑42 (MQ=255) gATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAac > 1:433168/1‑42 (MQ=255) gATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCtggtttgtggttt > 1:2907265/1‑71 (MQ=255) aTGGCCTGATGGGCGGTGGCTTCCTTTATACCAATGAAAATACCCTGTCGCTTGGGCtggtttgtggtttg > 1:807686/1‑71 (MQ=38) aTGGCCTGATGGGCGGTGGCTTCCTTTATACCAATGAAAATACCCTGTCGCTTGGGCtggtttgtggtttg > 1:4414795/1‑71 (MQ=38) aTGGCCTGATGGGCGGTGGCTTCCTTTATACCAATGAAAATACCCTGTCGCTTGGGCtggtttgtggtttg > 1:4288489/1‑71 (MQ=38) aTGGCCTGATGGGCGGTGGCTTCCTTTATACCAATGAAAATACCCTGTCGCTTGGGCtggtttgtggtttg > 1:3046272/1‑71 (MQ=38) aTGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCt < 1:4756324/58‑1 (MQ=255) ggCCTGATGGGCGGCGGCTTCCTTTATACCAATGaaaa < 1:2487502/38‑1 (MQ=255) ggCCTGATGGGCGGCGGCTTCCTTTATACCAATGaaaa < 1:2499003/38‑1 (MQ=255) tGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGcatcat > 1:4999476/1‑71 (MQ=255) gATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGcatc < 1:3787565/68‑1 (MQ=255) gATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGcatc < 1:3912033/68‑1 (MQ=255) gATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGcatc < 1:3906058/68‑1 (MQ=255) aTGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTc < 1:3832463/41‑1 (MQ=255) aTGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTc < 1:351899/41‑1 (MQ=255) aTGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTc < 1:195986/41‑1 (MQ=255) aTGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCtggtttgtg > 1:4870943/1‑58 (MQ=255) tGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTc < 1:1498499/40‑1 (MQ=255) gggCGGCGTCTTCCTTTATACCAATGAAAACACCCt < 1:5060987/36‑1 (MQ=255) gggCGGCGGCTTCCTTTATACCAATGAAAACACCCt < 1:4052999/36‑1 (MQ=255) gggCGGCGGCTTCCTTTATACCAATGAAAACACCCt < 1:4886811/36‑1 (MQ=255) gggCGGCGGCTTCCTTTATACCAATGAAAACACCCt < 1:685528/36‑1 (MQ=255) gggCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCtggtttgtggtttg > 1:4677918/1‑61 (MQ=255) ggcggcggcTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTg < 1:1841493/69‑1 (MQ=255) gcggcggcTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCAt < 1:4084712/71‑1 (MQ=255) ctgcggcTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCAt < 1:319157/68‑1 (MQ=255) cggcTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGTCtggtt < 1:664019/46‑1 (MQ=255) cggcTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCtggtt < 1:1121203/46‑1 (MQ=255) | AGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCAT > Exported/42459‑42592 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |