Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,965,079	T→C	11.3%	I187I (ATT→ATC)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,965,079	0	T	C	11.3%	167.1 / 11.2	62	I187I (ATT→ATC)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (17/38);  new base C (4/3);  total (21/41)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

ATAAACGTTTCTACGCGACCCGTGATATTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTT  >  Exported/2965009‑2965147                                                                       |                                                                     aTAAACGTTTCTACGCGACCCGTGATATTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTAtt                                                                      <  1:1686251/71‑1 (MQ=255) aTAAACGTTTCTACGCGACCCGTGATATTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTAtt                                                                      <  1:3494744/71‑1 (MQ=255)  tAAACGTTTCTACGCGACCCGTGATATTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTAtt                                                                      <  1:2811801/70‑1 (MQ=255)        tttCTACGCGACCCGTGATATTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCg                                                                <  1:1218432/70‑1 (MQ=255)        tttCTACGCGACCCGTGATATTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCg                                                                <  1:452445/70‑1 (MQ=255)          tCTACGCGACCCGTGATATTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaa                                                              <  1:2186723/70‑1 (MQ=255)                            ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaaga                                                            <  1:2639714/54‑1 (MQ=255)                            ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaaga                                                            <  1:3113423/54‑1 (MQ=255)                            ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaaga                                                            <  1:2363939/54‑1 (MQ=255)                            ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaaga                                                            <  1:3529885/54‑1 (MQ=255)                            ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaaga                                                            <  1:1183175/54‑1 (MQ=255)                            ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaaga                                                            <  1:3936596/54‑1 (MQ=255)                            ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaaga                                                            <  1:176713/54‑1 (MQ=255)                            ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaaga                                                            <  1:4580373/54‑1 (MQ=255)                            ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                           <  1:3981556/71‑1 (MQ=255)                            ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                           <  1:172204/71‑1 (MQ=255)                            ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                           <  1:2187430/71‑1 (MQ=255)                            ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                           <  1:4275318/71‑1 (MQ=255)                            ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                           <  1:4556448/71‑1 (MQ=255)                              aCCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTgg                                          <  1:346309/70‑1 (MQ=255)                              aCCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTgg                                          <  1:5040416/70‑1 (MQ=255)                              aCCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTgg                                          <  1:3606100/70‑1 (MQ=255)                               ccGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAc                                        >  1:4813304/1‑71 (MQ=255)                                  cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgcg                                     >  1:248198/1‑71 (MQ=255)                                  cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgcg                                     >  1:3720867/1‑71 (MQ=255)                                  cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgcg                                     >  1:4779475/1‑71 (MQ=255)                                  cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgc                                      >  1:3699205/1‑70 (MQ=255)                                  cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcg                                       >  1:5087612/1‑69 (MQ=255)                                    aCCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCt                                   <  1:2012054/71‑1 (MQ=255)                                        tgtaCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgc                                      <  1:4562786/61‑1 (MQ=255)                                        tGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGtt                               <  1:2211737/71‑2 (MQ=255)                                        tGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTg                               <  1:1541262/71‑1 (MQ=255)                                           aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                            <  1:2133124/71‑1 (MQ=255)                                           aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                            <  1:3661095/71‑1 (MQ=255)                                           aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                            <  1:3819659/71‑1 (MQ=255)                                           aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                            <  1:4317285/71‑1 (MQ=255)                                           aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                            <  1:1747669/71‑1 (MQ=255)                                               cATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGAc                         >  1:726518/1‑70 (MQ=255)                                               cATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACg                        >  1:2423087/1‑71 (MQ=255)                                               cATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGCAAGGTCTGGa                                         >  1:4767651/1‑54 (MQ=255)                                                   ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                     <  1:933279/70‑1 (MQ=255)                                                   ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                     <  1:748009/70‑1 (MQ=255)                                                   ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                     <  1:749094/70‑1 (MQ=255)                                                   ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                     <  1:2422454/70‑1 (MQ=255)                                                       tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                >  1:627910/1‑70 (MQ=255)                                                       tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                >  1:3765659/1‑70 (MQ=255)                                                       tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                >  1:4654633/1‑70 (MQ=255)                                                       tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                >  1:5139499/1‑70 (MQ=255)                                                        gATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTgg               >  1:2511946/1‑71 (MQ=255)                                                           cACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                 >  1:542705/1‑50 (MQ=255)                                                           cACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                 >  1:509576/1‑50 (MQ=255)                                                           cACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                 >  1:5088838/1‑50 (MQ=255)                                                             ccGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAgc          <  1:1572932/71‑1 (MQ=255)                                                             ccGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAgc          <  1:1564036/71‑1 (MQ=255)                                                              cggctcgATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGTCGGCAgcg         <  1:5072468/68‑1 (MQ=255)                                                              cggctcgATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGTCGGCAgcg         <  1:5096278/68‑1 (MQ=255)                                                              cggctcgATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGTCGGCAgcg         <  1:4421560/68‑1 (MQ=255)                                                                ccTCTATTCTGGCGAAGAAACTAGCGGAAGGACTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAgcggc       >  1:1677587/1‑71 (MQ=255)                                                                  tctATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                   >  1:5198074/1‑57 (MQ=255)                                                                    tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                <  1:1698747/42‑1 (MQ=255)                                                                    tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                <  1:3360109/42‑1 (MQ=255)                                                                    tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                <  1:3353852/42‑1 (MQ=255)                                                                    tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                <  1:1788533/42‑1 (MQ=255)                                                                    tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGt   >  1:3394182/1‑71 (MQ=255)                                                                    tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGt   >  1:3431812/1‑71 (MQ=255)                                                                      ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGa                          <  1:2363989/46‑1 (MQ=255)                                                                      ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGa                          <  1:4703971/46‑1 (MQ=255)                                                                      ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGa                          <  1:3111659/46‑1 (MQ=255)                                                                      ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGtt  <  1:4676491/70‑1 (MQ=255)                                                                       tCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                 >  1:3567664/1‑38 (MQ=255)                                                                       tCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                 >  1:752776/1‑38 (MQ=255)                                                                       |                                                                     ATAAACGTTTCTACGCGACCCGTGATATTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTT  >  Exported/2965009‑2965147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑