Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,880,895 C→T 8.4% L71L (TTG→TTA ytfF ← predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,880,8950CT 8.4% 468.3 / 22.8 156L71L (TTG→TTAytfFpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (76/66);  new base T (4/9);  total (81/75)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

Only 100 of 168 total aligned reads displayed.

GCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAATGGGCAGC  >  Exported/2880827‑2880964
                                                                    |                                                                     
gcgcCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc                                                                       >  1:2991855/1‑69 (MQ=255)
   ccAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGc                                                                  >  1:5071882/1‑71 (MQ=255)
   ccAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGc                                                                  >  1:4447230/1‑71 (MQ=255)
      gTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc                                                                       <  1:4999258/63‑1 (MQ=255)
      gTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc                                                                       <  1:2747728/63‑1 (MQ=255)
      gTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc                                                                       <  1:4414921/63‑1 (MQ=255)
      gTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc                                                                       <  1:682261/63‑1 (MQ=255)
      gTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc                                                                       <  1:3654813/63‑1 (MQ=255)
         cGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTaa                                                             >  1:4725206/1‑70 (MQ=255)
         cGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAg                                                            >  1:1475554/1‑71 (MQ=255)
         cGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAg                                                            >  1:3724160/1‑71 (MQ=255)
         cGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAg                                                            >  1:4955831/1‑71 (MQ=255)
         cGTTGAATGGCACTGGCAAGGCAGAAGTAAAAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAg                                                            >  1:2999366/1‑71 (MQ=255)
          gTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGc                                                           <  1:4371471/71‑1 (MQ=255)
            tGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGc                                                           >  1:1875012/1‑69 (MQ=255)
             gAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAg                                                        >  1:5173878/1‑71 (MQ=255)
             gAATGGCACTGGCAAGGAAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCa                                                         >  1:4121131/1‑70 (MQ=255)
                  gCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc                                                    >  1:3602458/1‑70 (MQ=255)
                  gCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc                                                    <  1:3981734/70‑1 (MQ=255)
                      tGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTa                                                              <  1:4301696/56‑1 (MQ=255)
                      tGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTa                                                              <  1:4633180/56‑1 (MQ=255)
                      tGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTa                                                              <  1:3864597/56‑1 (MQ=255)
                      tGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTa                                                              <  1:109793/56‑1 (MQ=255)
                      tGGCAAGGCAGAAGTAATAGATGAGGTTGCCCACCATAGTGAGCATCAAGGCGGTa                                                              <  1:3120409/56‑1 (MQ=255)
                        gCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAg                                                            >  1:1231804/1‑56 (MQ=255)
                        gCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAg                                                            >  1:3450843/1‑56 (MQ=255)
                        gCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAg                                                            >  1:2609040/1‑56 (MQ=255)
                        gCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAg                                                            >  1:2720337/1‑56 (MQ=255)
                        gCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACgcgc                                             >  1:1779278/1‑71 (MQ=255)
                          aaGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCa                                           <  1:4318205/71‑1 (MQ=255)
                          aaGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCa                                           <  1:5186876/71‑1 (MQ=255)
                            ggCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAAc                                         >  1:923530/1‑71 (MQ=255)
                             gCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACt                                        >  1:5022194/1‑71 (MQ=255)
                             gCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACt                                        <  1:502197/71‑1 (MQ=255)
                             gCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACt                                        >  1:2872604/1‑71 (MQ=255)
                                gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa                                                                     <  1:2310033/39‑1 (MQ=255)
                                gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa                                                                     <  1:832234/39‑1 (MQ=255)
                                gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa                                                                     <  1:1316368/39‑1 (MQ=255)
                                gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa                                                                     <  1:525441/39‑1 (MQ=255)
                                gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa                                                                     <  1:46228/39‑1 (MQ=255)
                                gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa                                                                     <  1:1927127/39‑1 (MQ=255)
                                gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa                                                                     <  1:2006335/39‑1 (MQ=255)
                                gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa                                                                     <  1:4764533/39‑1 (MQ=255)
                                gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa                                                                     <  1:2503308/39‑1 (MQ=255)
                                  aGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAgg                                                                   >  1:4496810/1‑39 (MQ=255)
                                  aGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgc                                   <  1:2841148/71‑1 (MQ=255)
                                  aGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgc                                   <  1:866256/71‑1 (MQ=255)
                                  aGTAATAGATGAGGTTGCCCATCATAGTGAGCATAAAGGCGGTAAGCCa                                                         >  1:4209417/1‑49 (MQ=255)
                                   gTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAAc                                         >  1:624921/1‑64 (MQ=255)
                                   gTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAAc                                         >  1:1382614/1‑64 (MQ=255)
                                   gTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgca                                  <  1:2362673/71‑1 (MQ=255)
                                        aGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgc                                   <  1:1482138/65‑1 (MQ=255)
                                              ggTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGt                       >  1:2911510/1‑71 (MQ=255)
                                              ggTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGt                       >  1:4028079/1‑71 (MQ=255)
                                               gTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgca                                  <  1:4024587/59‑1 (MQ=255)
                                               gTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgca                                  <  1:4255674/59‑1 (MQ=255)
                                               gTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgca                                  <  1:356019/59‑1 (MQ=255)
                                               gTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgca                                  <  1:2598408/59‑1 (MQ=255)
                                               gTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgca                                  <  1:1858310/59‑1 (MQ=255)
                                               gTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgca                                  <  1:1137731/59‑1 (MQ=255)
                                                 tGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTcc                     >  1:2872467/1‑70 (MQ=255)
                                                    ccATCATAGTGAGCATTAAGGCGGTAAGCCAGTCGCGACGCGCCAACTga                                      <  1:384823/50‑1 (MQ=255)
                                                    ccATCATAGTGAGCATTAAGGCGGTAAGCCAGTCGCGACGCGCCAACTga                                      <  1:1975150/50‑1 (MQ=255)
                                                    ccATCATAGTGAGCATTAAGGCGGTAAGCCAGTCGCGACGCGCCAACTga                                      <  1:5167801/50‑1 (MQ=255)
                                                    ccATCATAGTGAGCATTAAGGCGGTAAGCCAGTCGCGACGCGCCAACTga                                      <  1:710675/50‑1 (MQ=255)
                                                    ccATCATAGTGAGCATTAAGGCGGTAAGCCAGTCGCGACGCGCCAACTga                                      <  1:1114267/50‑1 (MQ=255)
                                                    ccATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagc                 >  1:5269/1‑71 (MQ=255)
                                                    ccATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagc                 >  1:3405579/1‑71 (MQ=255)
                                                    ccATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagc                 <  1:1805428/71‑1 (MQ=255)
                                                    ccATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagc                 >  1:1112328/1‑71 (MQ=255)
                                                    ccATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagc                 >  1:4455251/1‑71 (MQ=255)
                                                    ccATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagc                 >  1:4882384/1‑71 (MQ=255)
                                                     catcatAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagcc                <  1:1016417/71‑1 (MQ=255)
                                                     catcatAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagcc                <  1:2835249/71‑1 (MQ=255)
                                                      atcatAGTGAGCATTAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacg                                    >  1:2102664/1‑50 (MQ=255)
                                                      atcatAGTGAGCATTAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacg                                    >  1:1037512/1‑50 (MQ=255)
                                                        catAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg             >  1:753924/1‑71 (MQ=255)
                                                        catAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg             >  1:1747227/1‑71 (MQ=255)
                                                        catAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg             >  1:1875296/1‑71 (MQ=255)
                                                        catAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg             >  1:4564795/1‑71 (MQ=255)
                                                         atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCaa                                          >  1:3932611/1‑41 (MQ=255)
                                                         atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg             >  1:2680042/1‑70 (MQ=255)
                                                         atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg             >  1:4769014/1‑70 (MQ=255)
                                                         atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg             >  1:3526994/1‑70 (MQ=255)
                                                         atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg             >  1:2730042/1‑70 (MQ=255)
                                                         atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg             >  1:724673/1‑70 (MQ=255)
                                                         atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGc            <  1:4501516/71‑1 (MQ=255)
                                                         atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGc            <  1:2095637/71‑1 (MQ=255)
                                                          tAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGc            >  1:314478/1‑70 (MQ=255)
                                                            gTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAAt         <  1:2437534/71‑1 (MQ=255)
                                                             tGAGCATCTAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAAt         <  1:309514/70‑1 (MQ=255)
                                                             tGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAAt         <  1:2528340/70‑1 (MQ=255)
                                                              gAGCATCAAGGCGGCAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAAt         <  1:2869330/69‑1 (MQ=255)
                                                               aGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAATggg      <  1:4059502/71‑1 (MQ=255)
                                                               aGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAATggg      <  1:3440046/71‑1 (MQ=255)
                                                                gCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAATggg      <  1:4984012/70‑1 (MQ=255)
                                                                 cATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagccag              >  1:3347718/1‑61 (MQ=255)
                                                                 cATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCa           <  1:4104329/64‑1 (MQ=255)
                                                                  aTCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCaa          >  1:3628238/1‑64 (MQ=255)
                                                                   tCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAATGGGCAgc  >  1:2151406/1‑71 (MQ=255)
                                                                    |                                                                     
GCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAATGGGCAGC  >  Exported/2880827‑2880964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: