Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,446,556 A→C 8.8% pseudogene (1500/1647 nt) ilvG ← Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,446,5560AC 8.8% 383.5 / 31.5 148pseudogene (1500/1647 nt)ilvGMutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
Reads supporting (aligned to +/- strand):  ref base A (55/80);  new base C (3/10);  total (58/90)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.86e-01

Only 100 of 166 total aligned reads displayed.

GACATGAAGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGG  >  Exported/2446490‑2446626
                                                                  |                                                                      
  cATGAAGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCTAGTGCCGCTTCAACCTGGTCTTTACGGGTg                                                                  <  1:3030057/71‑1 (MQ=255)
  cATGAAGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTg                                                                  <  1:5081994/71‑1 (MQ=255)
  cATGAAGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTg                                                                  <  1:1707754/71‑1 (MQ=255)
  cATGAAGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTg                                                                  <  1:3147632/71‑1 (MQ=255)
      aaGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGAtgt                                                              >  1:4109153/1‑71 (MQ=255)
      aaGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGAtgt                                                              <  1:3436715/71‑1 (MQ=255)
      aaGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGAtgt                                                              >  1:2272400/1‑71 (MQ=255)
      aaGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGAtgt                                                              <  1:3712772/71‑1 (MQ=255)
      aaGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGAtgt                                                              >  1:1966255/1‑71 (MQ=255)
      aaGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGAtgt                                                              >  1:701290/1‑71 (MQ=255)
        gCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATgtgt                                                            <  1:3683547/71‑1 (MQ=255)
            gTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGt                                                                   <  1:1312224/60‑1 (MQ=255)
            gTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGt                                                                   <  1:1336710/60‑1 (MQ=255)
            gTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGt                                                                   <  1:37592/60‑1 (MQ=255)
                 gCCCATCACTGTTCAGGATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAgt                                                    <  1:4392887/70‑2 (MQ=255)
                  cccATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGa                                                  <  1:3207369/71‑1 (MQ=255)
                   ccATCACTGTTCAGCATGTTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGAt                                                 <  1:1762832/71‑1 (MQ=255)
                   ccATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGAt                                                 <  1:3539280/71‑1 (MQ=255)
                    cATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCa                                                      >  1:2941602/1‑65 (MQ=255)
                    cATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCa                                                      >  1:3811951/1‑65 (MQ=255)
                    cATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCa                                                      >  1:1405186/1‑65 (MQ=255)
                    cATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCa                                                      >  1:3298248/1‑65 (MQ=255)
                     aTCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTCCGGGTGATgtgt                                                            <  1:1742786/58‑1 (MQ=255)
                     aTCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTCCGGGTGATgtgt                                                            <  1:1956312/58‑1 (MQ=255)
                     aTCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTCCGGGTGATgtgt                                                            <  1:4967732/58‑1 (MQ=255)
                     aTCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTCCGGGTGATgtgt                                                            <  1:3537266/58‑1 (MQ=255)
                     aTCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTCCGGGTGATgtgt                                                            <  1:3161039/58‑1 (MQ=255)
                      tCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACg                                                                      <  1:2033332/47‑1 (MQ=255)
                      tCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACg                                                                      <  1:2596847/47‑1 (MQ=255)
                      tCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACg                                                                      <  1:4797415/47‑1 (MQ=255)
                        aCTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCg                                             >  1:2070187/1‑70 (MQ=255)
                        aCTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCg                                             >  1:3002075/1‑70 (MQ=255)
                        aCTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCg                                             >  1:2657318/1‑70 (MQ=255)
                        aCTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCg                                             >  1:3502031/1‑70 (MQ=255)
                        aCTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCg                                             <  1:3052917/70‑1 (MQ=255)
                        aCTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCg                                             >  1:4071008/1‑70 (MQ=255)
                        aCTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCg                                             >  1:134028/1‑70 (MQ=255)
                        aCTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCg                                             <  1:4011570/70‑1 (MQ=255)
                          tGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTAc                                                                       <  1:5168406/42‑1 (MQ=255)
                          tGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTAc                                                                       <  1:43397/42‑1 (MQ=255)
                          tGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTAc                                                                       <  1:1368700/42‑1 (MQ=255)
                            ttCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTa                                                                        >  1:847591/1‑39 (MQ=255)
                            ttCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTa                                                                        >  1:1057140/1‑39 (MQ=255)
                            ttCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTa                                                                        >  1:4850870/1‑39 (MQ=255)
                            ttCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTa                                                                        >  1:1071874/1‑39 (MQ=255)
                            ttCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTa                                                                        >  1:124654/1‑39 (MQ=255)
                            ttCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTa                                                                        >  1:2949569/1‑39 (MQ=255)
                            ttCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTa                                                                        >  1:1584621/1‑39 (MQ=255)
                            ttCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTa                                                                        >  1:2206876/1‑39 (MQ=255)
                            ttCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAgg                                         <  1:5039651/70‑1 (MQ=255)
                            ttCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAgg                                         <  1:2732601/70‑1 (MQ=255)
                            ttCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAgg                                         >  1:5106639/1‑70 (MQ=255)
                            ttCAGCATGGTGACGAGTGCCGCTTCAACCTGGTCTTTa                                                                        >  1:3295899/1‑39 (MQ=255)
                                gCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGTGTGATGTGTTGGCCAGGGATGCCg                                             <  1:532653/62‑1 (MQ=255)
                                  aTGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGc                                  <  1:3453404/71‑1 (MQ=255)
                                  aTGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGc                                  <  1:462022/71‑1 (MQ=255)
                                  aTGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGc                                  <  1:3634251/71‑1 (MQ=255)
                                  aTGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGc                                  <  1:3175228/71‑1 (MQ=255)
                                  aTGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGc                                  <  1:794747/71‑1 (MQ=255)
                                  aTGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGc                                  <  1:1540276/71‑1 (MQ=255)
                                   tGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGc                                  >  1:4697660/1‑70 (MQ=255)
                                      tgtCGAGTGCCGCTTCAACCTGGTCTTTCCGGGTGATgtgt                                                            <  1:12542/41‑1 (MQ=255)
                                      tgtCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTaa                               >  1:2712951/1‑70 (MQ=255)
                                      tgtCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTaa                               >  1:1144165/1‑70 (MQ=255)
                                      tgtCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTaa                               >  1:4534957/1‑70 (MQ=255)
                                       gtCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAgg                                                    <  1:2511338/48‑1 (MQ=255)
                                       gtCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAgg                                                    <  1:210487/48‑1 (MQ=255)
                                       gtCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAgg                                                    <  1:3894638/48‑1 (MQ=255)
                                          gAGTGCCGCTTCAACCTGGTCTTTCCGGGTGATGTGTTGGCCATGGATGCCGAAGGCGCTGGCTAACAt                            >  1:3572957/1‑69 (MQ=255)
                                          gAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGAt                                                 <  1:2608898/48‑1 (MQ=255)
                                          gAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACAt                            >  1:838885/1‑69 (MQ=255)
                                          gAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACAt                            >  1:1946011/1‑69 (MQ=255)
                                          gAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACAt                            >  1:2425832/1‑69 (MQ=255)
                                                cGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAAt                    <  1:701800/71‑1 (MQ=255)
                                                cGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAAt                    <  1:2325849/71‑1 (MQ=255)
                                                cGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAAt                    <  1:2376660/71‑1 (MQ=255)
                                                  cTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCg                  <  1:4578853/71‑1 (MQ=255)
                                                  cTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCg                  <  1:3127978/71‑1 (MQ=255)
                                                  cTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCg                  <  1:425407/71‑1 (MQ=255)
                                                    tCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGa                                                  >  1:4712045/1‑37 (MQ=255)
                                                    tCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGa                                                  >  1:640938/1‑37 (MQ=255)
                                                    tCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGa                                                  >  1:892729/1‑37 (MQ=255)
                                                         cTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCt                                 <  1:3027547/49‑1 (MQ=255)
                                                          tGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCg                  <  1:2537136/63‑1 (MQ=255)
                                                           ggTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCg                  <  1:2122905/62‑1 (MQ=255)
                                                            gTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCgg        >  1:4837723/1‑71 (MQ=255)
                                                             tCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACAt                            <  1:1022866/50‑1 (MQ=255)
                                                             tCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACAt                            <  1:1990420/50‑1 (MQ=255)
                                                             tCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGt       <  1:1007363/71‑1 (MQ=255)
                                                             tCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGt       <  1:2832096/71‑1 (MQ=255)
                                                             tCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGt       <  1:1234187/71‑1 (MQ=255)
                                                              cTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGt       <  1:3342334/70‑1 (MQ=255)
                                                              cTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGt       >  1:1177576/1‑70 (MQ=255)
                                                                 tACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCt                                     >  1:2019570/1‑37 (MQ=255)
                                                                 tACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCt                                     >  1:142685/1‑37 (MQ=255)
                                                                 tACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCt                                     >  1:1673068/1‑37 (MQ=255)
                                                                 tACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCt                                     >  1:447430/1‑37 (MQ=255)
                                                                 tACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCt                                     >  1:402541/1‑37 (MQ=255)
                                                                 tACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAg    <  1:4109170/70‑1 (MQ=255)
                                                                  aCGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAggg  >  1:1109499/1‑71 (MQ=255)
                                                                  |                                                                      
GACATGAAGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGTAAGGG  >  Exported/2446490‑2446626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: