| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,385,964 | A→T | 13.4% | L56L (CTA→CTT) | metR → | DNA‑binding transcriptional activator, homocysteine‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,385,964 | 0 | A | T | 13.4% | 158.2 / 14.1 | 59 | L56L (CTA→CTT) | metR | DNA‑binding transcriptional activator, homocysteine‑binding |
| Reads supporting (aligned to +/- strand): ref base A (31/20); new base T (4/4); total (35/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCTCACCAGTTTAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTACACCGCAGGGAGAAATCCTGTTGCAACTGGCAAACCAGGTACTGCCGCAAATTAGC > Exported/2385895‑2386027 | gTCTCACCAGTTTAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTa > 1:4175068/1‑70 (MQ=255)gTCTCACCAGTTTAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTAc > 1:3608811/1‑71 (MQ=255) aCCAGTTTAGCGATCTGGAACAACGCCTTGGCTTCCTGCTATTTGTGCGTAAGAGCCAGCCGCTACGCt < 1:4692691/69‑1 (MQ=255) aCCAGTTTAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGc < 1:4421151/68‑1 (MQ=255) ccAGTTTAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa < 1:242924/71‑1 (MQ=255) ccAGTTTAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa < 1:720770/71‑1 (MQ=255) cAGTTTAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCtt < 1:4709587/68‑1 (MQ=255) aGTTTAGCGTTCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTa > 1:642158/1‑62 (MQ=255) aGTTTAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTa > 1:2843290/1‑62 (MQ=255) aGTTTAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTa > 1:2460218/1‑62 (MQ=255) aGTTTAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTa > 1:1175499/1‑62 (MQ=255) aGTTTAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTa > 1:3173875/1‑62 (MQ=255) aGTTTAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTa > 1:4315913/1‑62 (MQ=255) ttAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa > 1:1700211/1‑66 (MQ=255) ttAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa > 1:4649305/1‑66 (MQ=255) ttAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa > 1:438283/1‑66 (MQ=255) ttAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa > 1:1346449/1‑66 (MQ=255) ttAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa > 1:4364045/1‑66 (MQ=255) ttAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa > 1:4889021/1‑66 (MQ=255) ttAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa > 1:1781117/1‑66 (MQ=255) tGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTACACCGCAGGgaga > 1:3104413/1‑70 (MQ=255) gAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTACACCGCAGGga < 1:1846477/66‑1 (MQ=255) gAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTACACCGCAGGga < 1:3422439/66‑1 (MQ=255) gAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTACACCGCAGGga < 1:2987568/66‑1 (MQ=255) aacGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCtt < 1:4688231/49‑1 (MQ=255) aacGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCtt < 1:783167/49‑1 (MQ=255) aacGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCtt < 1:1649464/49‑1 (MQ=255) aacGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCtt < 1:613820/49‑1 (MQ=255) aacGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCtt < 1:178418/49‑1 (MQ=255) aacGCCTTGGCTTCCGGCTATTTGTGCGTAAAAGCCAGCCGCTTCGGTTTACTCCGCAGGGAGAAATCCTg < 1:4448841/71‑1 (MQ=255) aacGCCTTGGCTTCCGGCTATTTGTGCGTAAAAGCCAGCCGCTTCGCTTTACACCGCAGGGAGAAATCCTg < 1:780440/71‑1 (MQ=255) aacGCCTTGGCTTCCGGCTATTTGTGCGTAAAAGCCAGCCGCTTCGCTTTACACCGCAGGGAGAAATCCTg < 1:578227/71‑1 (MQ=255) aacGCCTTGGCTTCCGGCTATTTGTGCGTAAAAGCCAGCCGCTTCGCTTTACACCGCAGGGAGAAATCCTg < 1:1875468/71‑1 (MQ=255) acGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa > 1:108138/1‑50 (MQ=255) acGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa > 1:5110326/1‑50 (MQ=255) acGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa > 1:3859143/1‑50 (MQ=255) acGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa > 1:950770/1‑50 (MQ=255) acGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa > 1:2046033/1‑50 (MQ=255) acGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa > 1:1948650/1‑50 (MQ=255) acGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa > 1:2030769/1‑50 (MQ=255) cGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTa > 1:4228431/1‑42 (MQ=255) cGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa > 1:3357442/1‑49 (MQ=255) cGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTa > 1:3102332/1‑49 (MQ=255) gCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTACACCGc < 1:2733181/54‑1 (MQ=255) cTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTACACCGCAGGGAGAAATCCTGTTGCa < 1:2199478/71‑1 (MQ=255) ttGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTACACCGCAGGgaga > 1:1940155/1‑58 (MQ=255) ggCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTACACCGCAGGGAGAAATCCTGtt > 1:3912613/1‑65 (MQ=255) ggCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTACACCGCAGGGAGAAATCCTGtt > 1:2326966/1‑65 (MQ=255) ggCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTACACCGCAGGGAGAAATCCTGtt > 1:4546835/1‑65 (MQ=255) ggCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTACACCGCAGGGAGAAATCCTGTTGCAAc < 1:1313580/70‑1 (MQ=255) ggCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTACACCGCAGGGAGAAATCCTGTTGCAACt > 1:2409197/1‑71 (MQ=255) ggCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTACACCGCAGGGAGAAATCCTGTTGCAACt > 1:47720/1‑71 (MQ=255) gccGCTTCGCTTTACACCGCAGGGAGAAATCCTGTTGCAACTGGCAAATCAGGTGCTGCCGCAAATTAGc > 1:4392339/1‑70 (MQ=255) gccGCTTCGCTTTACACCGCAGGGAGAAATCCTGTTGCAACTGGCAAATCAGGTGCTGCCGCAAATTAGc > 1:3062036/1‑70 (MQ=255) gccGCTTCGCTTTACACCGCAGGGAGAAATCCTGTTGCAACTGGCAAATCAGGTGCTGCCGCAAATTAGc > 1:830278/1‑70 (MQ=255) gccGCTTCGCTTTACACCGCAGGGAGAAATCCTGTTGCAACTGGCAAATCAGGTGCTGCCGCAAATTAGc > 1:2606519/1‑70 (MQ=255) gCTACGCTTTACACCGCAGGGAGAAATCCTGTTGCa < 1:2788119/36‑1 (MQ=255) gCTACGCTTTACACCGCAGGGAGAAATCCTGTTGCa < 1:4238889/36‑1 (MQ=255) gCTACGCTTTACACCGCAGGGAGAAATCCTGTTGCa < 1:4178148/36‑1 (MQ=255) gCTACGCTTTACACCGCAGGGAGAAATCCTGTTGCa < 1:2668242/36‑1 (MQ=255) tACGCTTTACACCGCAGGGAGAAATCCTGTTGCAAc > 1:1368081/1‑36 (MQ=255) tACGCTTTACACCGCAGGGAGAAATCCTGTTGCAAc > 1:1248060/1‑36 (MQ=255) tACGCTTTACACCGCAGGGAGAAATCCTGTTGCAAc > 1:3648176/1‑36 (MQ=255) tACGCTTTACACCGCAGGGAGAAATCCTGTTGCAAc > 1:443357/1‑36 (MQ=255) tACGCTTTACACCGCAGGGAGAAATCCTGTTGCAAc > 1:3152684/1‑36 (MQ=255) tACGCTTTACACCGCAGGGAGAAATCCTGTTGCAAc > 1:1004273/1‑36 (MQ=255) tACGCTTTACACCGCAGGGAGAAATCCTGTTGCAAc > 1:995511/1‑36 (MQ=255) aCGCTTTACACCGCAGGGAGAAATCCTGTTGCAACTGGCAAAc > 1:813540/1‑43 (MQ=255) aCGCTTTACACCGCAGGGAGAAATCCTGTTGCAACTGGCAAAc > 1:459645/1‑43 (MQ=255) aCGCTTTACACCGCAGGGAGAAATCCTGTTGCAACTGGCAAAc > 1:407619/1‑43 (MQ=255) aCGCTTTACACCGCAGGGAGAAATCCTGTTGCAACTGGCAAAc > 1:4062615/1‑43 (MQ=255) aCGCTTTACACCGCAGGGAGAAATCCTGTTGCAACTGGCAAAc > 1:3918116/1‑43 (MQ=255) aCGCTTTACACCGCAGGGAGAAATCCTGTTGCAACTGGCAAAc > 1:3487087/1‑43 (MQ=255) | GTCTCACCAGTTTAGCGATCTGGAACAACGCCTTGGCTTCCGGCTATTTGTGCGTAAGAGCCAGCCGCTACGCTTTACACCGCAGGGAGAAATCCTGTTGCAACTGGCAAACCAGGTACTGCCGCAAATTAGC > Exported/2385895‑2386027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |