Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,245,0210AG53.2% 90.0 / inf 456C12C (TGT→TGC) aphAacid phosphatase AphA
Reads supporting (aligned to +/- strand):  ref base A (101/111);  new base G (115/126);  total (217/239)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 478 total aligned reads displayed.

GACGCCCTGCGAGGCTATTTTCAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGAAAGGAAGGATTATCTGCGGTTTTTGTG  >  DH1/4244873‑4245171
                                                                                                                                                    |                                                                                                                                                       
gACGCCCTGCGAGGCTATTTTCAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATaagca                                                                                                                                                       <  7:854392/151‑1 (MQ=255)
 aCGCCCTGCGAGGCTATTTTCAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATaagca                                                                                                                                                       >  2:1410151/1‑150 (MQ=255)
 aCGCCCTGCGAGGCTATTTTCAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATaaacaa                                                                                                                                                      <  8:721579/151‑1 (MQ=255)
     ccTGCGAGGCTATTTTCAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACgg                                                                                                                                                 >  3:361977/1‑151 (MQ=255)
      cTGCGAGGCTATTTTCAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGc                                                                                                                                                >  1:792777/1‑151 (MQ=255)
        gCGAGGCTATTTTCATTTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCa                                                                                                                                               <  7:1681700/150‑1 (MQ=255)
            ggCTATTTTCAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCTACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGAt                                                                                                                                          >  4:282500/1‑151 (MQ=255)
               tATTTTCAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGc                                                                                                                                       >  4:374097/1‑151 (MQ=255)
               tATTTTCAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGc                                                                                                                                       >  4:1870267/1‑151 (MQ=255)
                 ttttCAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCt                                                                                                                                     >  6:1255420/1‑151 (MQ=255)
                  tttCAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCt                                                                                                                                     <  6:283926/150‑1 (MQ=255)
                  tttCAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCt                                                                                                                                     <  5:536022/150‑1 (MQ=255)
                  tttCAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCt                                                                                                                                     <  7:1575214/150‑1 (MQ=255)
                   ttCAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCtgt                                                                                                                                   >  3:1844352/1‑151 (MQ=255)
                   ttCAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCtg                                                                                                                                    <  5:1740223/150‑1 (MQ=255)
                     cAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCtgtgt                                                                                                                                 <  4:925877/151‑1 (MQ=255)
                     cAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCtgtg                                                                                                                                  <  4:609762/150‑1 (MQ=255)
                      aaTTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCtgtgtg                                                                                                                                >  2:1069972/1‑151 (MQ=255)
                       aTTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGa                                                                                                                               <  2:402321/151‑1 (MQ=255)
                         ttGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATc                                                                                                                             >  4:1663180/1‑151 (MQ=255)
                           gtgCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCtt                                                                                                                           <  8:1872415/151‑1 (MQ=255)
                             gCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTgc                                                                                                                         <  2:1504703/151‑1 (MQ=255)
                              cGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTgcg                                                                                                                        <  4:1750682/151‑1 (MQ=255)
                               gACCGAAACCCTATGACTGGGTGCCTGTTCAGCAAGACTGGCAACGTTAGTCCGAGGGTTAAGCGGGGAAGTAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATCAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTgcgc                                                                                                                       <  3:1125356/151‑1 (MQ=255)
                               gACCGAAACCCAATGAATGGGTGCCTGTTGAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCTCTGATTGCCTGTGTGATCTTgcgc                                                                                                                       >  2:1757294/1‑151 (MQ=255)
                               gACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTgcgc                                                                                                                       >  7:579590/1‑151 (MQ=255)
                               gACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTgcgc                                                                                                                       >  5:30973/1‑151 (MQ=255)
                                   gAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAATCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCatat                                                                                                                   >  8:530689/1‑151 (MQ=255)
                                   gAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCatat                                                                                                                   >  1:674312/1‑151 (MQ=255)
                                    aaaCCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATAtt                                                                                                                  >  4:830464/1‑151 (MQ=255)
                                     aaCCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATAttt                                                                                                                 <  5:453793/151‑1 (MQ=255)
                                       cccAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATAttttt                                                                                                               >  2:75352/1‑151 (MQ=255)
                                           atgcatgGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGAGGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGAGCTTGCGCATATTTTTCCCt                                                                                                           <  4:1563332/151‑1 (MQ=255)
                                           atgaatgGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGGGATCTTGCGCATATTTTTCCCt                                                                                                           >  1:209780/1‑151 (MQ=255)
                                              aatgGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTaa                                                                                                         >  1:184245/1‑150 (MQ=255)
                                              aatgGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTaaa                                                                                                        <  8:131051/151‑1 (MQ=255)
                                              aatgGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGGGAAGGAGATGAGGCCAGCGCAACAGCGGAACTGTTTAGACCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTaaa                                                                                                        <  2:659718/151‑1 (MQ=255)
                                               atgGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAAt                                                                                                       <  5:1403106/151‑1 (MQ=255)
                                                 gggTGGCGGTTCTGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCACGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAg                                                                                                     <  6:362160/151‑1 (MQ=255)
                                                 gggTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCACTAAAt                                                                                                       >  2:1649310/1‑149 (MQ=255)
                                                 gggTGCCTGTTCAGCAAGCCTGGCAACGGTAGGCCCAGGGTTAAGCGGTGAAGGAGTTGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTTTGATCTTGCGCATATTTTTCCCTAAATAg                                                                                                     <  8:826783/151‑1 (MQ=255)
                                                    tGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTc                                                                                                   >  7:1329672/1‑150 (MQ=255)
                                                      ccTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGt                                                                                                >  8:40389/1‑151 (MQ=255)
                                                       cTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGt                                                                                                >  1:317642/1‑150 (MQ=255)
                                                               cAAACCTGGCAACGGTAGTGCCAGGGTTAATCGGGGCACGAGCTGCCGCCCCGGGAAAACCGGAACTTTTTTCACGGAACAATAAACAACACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGaa                                                                                        <  1:930619/151‑1 (MQ=255)
                                                                aaGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAAc                                                                                      >  6:493792/1‑151 (MQ=255)
                                                                aaGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAAc                                                                                      >  3:154805/1‑151 (MQ=255)
                                                                  gCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACtt                                                                                    >  6:112898/1‑151 (MQ=255)
                                                                    cTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACtttt                                                                                  >  2:966477/1‑151 (MQ=255)
                                                                      ggCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTa                                                                                <  4:517758/151‑1 (MQ=255)
                                                                       gCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTAt                                                                               <  8:614802/151‑1 (MQ=255)
                                                                        cAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACAGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATAATTTTCCCTAAATAGTCAGTATGTTGAAAGATTATAtt                                                                              >  4:1130393/1‑151 (MQ=255)
                                                                         aaCGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGTAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATtg                                                                             <  5:1569905/151‑1 (MQ=255)
                                                                         aaCGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATtg                                                                             >  8:1490841/1‑151 (MQ=255)
                                                                              tAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATtgtg                                                                           >  7:192127/1‑148 (MQ=255)
                                                                               aGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATtgtg                                                                           >  3:660011/1‑147 (MQ=255)
                                                                               aGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGAt                                                                       <  1:1529404/151‑1 (MQ=255)
                                                                                 tCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGAttt                                                                     >  3:724711/1‑151 (MQ=255)
                                                                                  cccAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGAttt                                                                     <  2:1262353/150‑1 (MQ=255)
                                                                                  cccAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATttg                                                                    >  5:863181/1‑151 (MQ=255)
                                                                                      gggTTAAGCGTTGAAGGAGATGAGGCCATTGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGc                                                                <  7:1217067/151‑1 (MQ=255)
                                                                                       ggTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGc                                                                >  6:809810/1‑150 (MQ=255)
                                                                                        gTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCACTAAATAGTCAGTATGTTGAAACTATTTATTGTGATATTTGTTGCa                                                               >  6:709710/1‑150 (MQ=255)
                                                                                         ttAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCaa                                                              >  7:1056580/1‑150 (MQ=255)
                                                                                           aaGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCaaaaa                                                           <  3:1339370/151‑1 (MQ=255)
                                                                                           aaGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCaaaa                                                            >  6:1871889/1‑150 (MQ=255)
                                                                                                  gAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTg                                                    <  1:1349868/151‑1 (MQ=255)
                                                                                                   aaGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGa                                                   <  7:1753720/151‑1 (MQ=255)
                                                                                                   aaGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTg                                                    >  6:1728690/1‑150 (MQ=255)
                                                                                                      gagaTGATGCCAGGGCAACAGGGTAACTGTTTATAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTTATCTTGCGCATATTTTTCCCTAAATAGTCATTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCa                                                <  5:1823844/151‑1 (MQ=255)
                                                                                                       agaTGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTTATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCAt                                               <  7:615802/151‑1 (MQ=255)
                                                                                                       agaTGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCAt                                               <  8:1003028/151‑1 (MQ=255)
                                                                                                       agaTGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCAt                                               >  2:564358/1‑151 (MQ=255)
                                                                                                         aTGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATa                                              >  5:1307785/1‑150 (MQ=255)
                                                                                                            aGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGc                                          >  6:133479/1‑151 (MQ=255)
                                                                                                              gCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCa                                        <  5:1055350/151‑1 (MQ=255)
                                                                                                              gCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCa                                        <  1:427126/151‑1 (MQ=255)
                                                                                                                cAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGc                                      >  5:1190376/1‑151 (MQ=255)
                                                                                                                cAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGc                                      >  1:1644366/1‑151 (MQ=255)
                                                                                                                     cAACAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCg                                 >  3:174712/1‑151 (MQ=255)
                                                                                                                      aaCAGCGGAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCgg                                >  6:157936/1‑151 (MQ=255)
                                                                                                                      aaCAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTTATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCgc                                <  1:1000099/151‑2 (MQ=255)
                                                                                                                      aaCAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCgg                                <  5:721644/151‑1 (MQ=255)
                                                                                                                          gCGGAACTGTCTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCGGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGaa                            <  2:1827835/151‑1 (MQ=255)
                                                                                                                            ggAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGAaag                          <  6:1017662/151‑1 (MQ=255)
                                                                                                                            ggAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGAaag                          <  3:417248/151‑1 (MQ=255)
                                                                                                                             gAACTGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGAaagg                         <  3:960085/151‑1 (MQ=255)
                                                                                                                              aaCTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGAaagg                         >  6:1390223/1‑150 (MQ=255)
                                                                                                                               aCTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGAaaggaa                       <  2:1175140/151‑1 (MQ=255)
                                                                                                                                 tGTTTAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGAaaggaagg                     >  7:410104/1‑151 (MQ=255)
                                                                                                                                  gTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGAaaggaagg                     <  8:113059/150‑1 (MQ=255)
                                                                                                                                   ttttGAGAGAACAATAAACAT‑ACGGGAGTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGAAaggaagga                    <  6:923377/150‑1 (MQ=255)
                                                                                                                                     tAGAGCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCCTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGAAAGGAAGGAtt                  >  5:599709/1‑150 (MQ=255)
                                                                                                                                     tAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCGCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGCGGAAAGGAAGGAtt                  >  4:1242816/1‑150 (MQ=255)
                                                                                                                                      agagCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGAAAGGAAGGATTAt                >  6:204964/1‑151 (MQ=255)
                                                                                                                                      agagCGAACAATAAGCAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGAAAGGAAGGATTAt                <  3:282147/151‑1 (MQ=255)
                                                                                                                                          cGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGAAAGGAAGGATTATCTGc            >  1:1103154/1‑151 (MQ=255)
                                                                                                                                                  aaacaa‑acGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGAAAGGAAGGATTATCTGCGGTTTTtg    >  4:1405446/1‑151 (MQ=255)
                                                                                                                                                   aacaa‑acGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGCAAAGCAAGGATTATCTCCGGTTTTtgt   >  8:1635672/1‑151 (MQ=255)
                                                                                                                                                    acaa‑acGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGAAAGGAAGGATTATCTGCGGTTTTtgtg  <  5:1532250/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
GACGCCCTGCGAGGCTATTTTCAATTTGTGCGACCGAAACCCAATGAATGGGTGCCTGTTCAGCAAGCCTGGCAACGTTAGTCCCAGGGTTAAGCGGTGAAGGAGATGAGGCCAGGGCAACAGCGGAACTGTTTAGAGCGAACAATAAACAA‑ACGGCACTGATTGCCTGTGTGATCTTGCGCATATTTTTCCCTAAATAGTCAGTATGTTGAAACTTTTTATTGTGAGATTTGTTGCAAAAACCATCTGACCATAACGCCAGCTGCCGGGGAAAGGAAGGATTATCTGCGGTTTTTGTG  >  DH1/4244873‑4245171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑