Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,026,7830AG53.5% 133.3 / inf 560C269R (TGT→CGT) lptFLPS export ABC transporter permease LptF
Reads supporting (aligned to +/- strand):  ref base A (139/121);  new base G (141/158);  total (281/279)
Fisher's exact test for biased strand distribution p-value = 1.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01

Only 100 of 572 total aligned reads displayed.

CGATTTCAGGGAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCGGAAATCACGTAA  >  DH1/4026634‑4026933
                                                                                                                                                     |                                                                                                                                                      
 gATTTCAGGGAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGcc                                                                                                                                                      >  6:419240/1‑151 (MQ=255)
 gATTTCAGGGAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACAcc                                                                                                                                                      >  7:1510254/1‑151 (MQ=255)
    ttCAGGGAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCcagt                                                                                                                                                   >  7:40213/1‑151 (MQ=255)
      cAGGTAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGGATCGACAGTACGCGTCGCTGACGTGGGTTATCCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCcagtt                                                                                                                                                  <  4:1241656/150‑1 (MQ=255)
      cAGGGAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCcagttc                                                                                                                                                 <  4:1676814/151‑1 (MQ=255)
      cAGGGAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCcagtt                                                                                                                                                  <  1:1614859/150‑1 (MQ=255)
      cAGGGAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCACTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCcagttc                                                                                                                                                 >  5:263065/1‑151 (MQ=255)
        gggAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCcagttcag                                                                                                                                               <  7:883837/151‑1 (MQ=255)
        gggAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACcagttc                                                                                                                                                 >  2:1353673/1‑149 (MQ=255)
         ggAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATTAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCcagttcagt                                                                                                                                              <  4:1772145/151‑1 (MQ=255)
         ggAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACcagttcagt                                                                                                                                              >  3:916614/1‑151 (MQ=255)
            ggTCTGGATCAGGAAGAAAAGTAGATACAGCAGCAGGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCt                                                                                                                                           <  8:1889404/151‑1 (MQ=255)
                tGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTgcgc                                                                                                                                       >  8:831086/1‑151 (MQ=255)
                 ggATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTgc                                                                                                                                         <  1:980283/148‑1 (MQ=255)
                 ggATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGcgcg                                                                                                                                      >  8:1625046/1‑151 (MQ=255)
                 ggATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTgcg                                                                                                                                        <  7:1323055/149‑1 (MQ=255)
                    tCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGc                                                                                                                                   >  4:1129366/1‑151 (MQ=255)
                    tCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGc                                                                                                                                   >  1:85181/1‑151 (MQ=255)
                     cAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCa                                                                                                                                  <  3:1796000/151‑1 (MQ=255)
                     cAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGAAACACCAGTGCAGTTCTGCGCGacc                                                                                                                                   >  6:284132/1‑148 (MQ=255)
                     cAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCAGCGACAGTACGCGTCCCTGACGTGGGTTAACAACGCTCAGCGGTAGGACCATAAGTGCCTTCATAACAACGGTGAATACCACGGTGATACGCCAGTACAGTTCTgcgcgcgc                                                                                                                                   >  3:72687/1‑150 (MQ=255)
                          agaaAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATc                                                                                                                             >  7:996178/1‑151 (MQ=255)
                           gagaaGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCg                                                                                                                            >  3:104354/4‑151 (MQ=255)
                           gaaAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCg                                                                                                                            >  3:1517146/1‑151 (MQ=255)
                            aaaaGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGCTCgg                                                                                                                           >  7:1615412/1‑151 (MQ=255)
                             aaaGTAGATACAGCAGCATGGCTGGCAGCATCGCCAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAACCACGGTGAAGACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGgt                                                                                                                          >  3:1389352/1‑151 (MQ=255)
                                    aTACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAgtg                                                                                                                   >  8:1099139/1‑151 (MQ=255)
                                     tACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTCACGTGGGTTAACCACGCTCAGCGGTACGCCCAGAAGTGCCATCATAACCACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAgtgt                                                                                                                  >  7:1133497/1‑151 (MQ=255)
                                      aCAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGtt                                                                                                                 <  1:501735/151‑1 (MQ=255)
                                       cagcagCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTc                                                                                                                >  5:1077425/1‑151 (MQ=255)
                                       cagcagCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTc                                                                                                                >  2:1090796/1‑151 (MQ=255)
                                          cagcaTGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCcac                                                                                                             <  6:23798/151‑1 (MQ=255)
                                           agcaTGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCcac                                                                                                             >  7:550481/1‑150 (MQ=255)
                                           agcaTGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCcac                                                                                                             >  8:487185/1‑150 (MQ=255)
                                           agcaTGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGAGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGATCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCcac                                                                                                             >  6:1494444/1‑150 (MQ=255)
                                            gcaTGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAACTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACaa                                                                                                           <  2:329426/151‑1 (MQ=255)
                                              aTGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAAtg                                                                                                         <  8:146292/151‑1 (MQ=255)
                                                 gctggcAGCATCGACAGTACGCGTCCCTGACGGGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGGGTCAGTGTTCCACAATGTgc                                                                                                      <  7:1428836/151‑1 (MQ=255)
                                                   gggcAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTgcgc                                                                                                    <  8:411316/150‑1 (MQ=255)
                                                    ggcAGGATCGACAGGACGCGTCCCTGACGTGGGTTAACCACGCTCATGGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTGCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTgcgc                                                                                                    <  4:733735/150‑1 (MQ=255)
                                                     gcagcaTCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCAt                                                                                                  <  1:346140/151‑1 (MQ=255)
                                                     gcagcaTCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCAt                                                                                                  <  2:266987/151‑1 (MQ=255)
                                                     gcagcaTCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCAGCATAAACACGGTGAATACCAACGTGATACACCAGTGCAGTTCTGCGCGCGCACGAGCGGTCTCAGGGTTCCACACTGTGCGCAt                                                                                                  >  7:331829/1‑151 (MQ=255)
                                                      cagcaTCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATg                                                                                                 >  5:533124/1‑151 (MQ=255)
                                                          aTCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTcc                                                                                              >  3:1195550/1‑150 (MQ=255)
                                                            cGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACTATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCAt                                                                                            <  5:737688/150‑1 (MQ=255)
                                                               cAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTgg                                                                                        >  2:185724/1‑151 (MQ=255)
                                                                aGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATTATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGt                                                                                       >  8:1316460/1‑151 (MQ=255)
                                                                aGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGt                                                                                       >  8:72563/1‑151 (MQ=255)
                                                                 gTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTc                                                                                      <  6:1034123/151‑1 (MQ=255)
                                                                   aCGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCgg                                                                                    >  4:888100/1‑151 (MQ=255)
                                                                       gTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATc                                                                                >  2:856477/1‑151 (MQ=255)
                                                                            tGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCg                                                                           >  2:767860/1‑151 (MQ=255)
                                                                            tGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCg                                                                           >  2:528675/1‑151 (MQ=255)
                                                                              aggTGGGTTAACGACGCTCAGCGGTACGAGCATAAGTGGCATCATAAACACGGTGAATACCAACGTTTTACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTGCATCTGGTCGGTATCGTTCggg                                                                         <  3:1669319/149‑1 (MQ=255)
                                                                               ggTGGTTTAACGACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTGCTGCGCGAGCACGATCTGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCTGTATCGTTCGGGt                                                                        <  4:560160/150‑1 (MQ=255)
                                                                               cGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGt                                                                        >  6:703154/1‑151 (MQ=255)
                                                                                   ggTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCg                                                                      >  5:689500/1‑149 (MQ=255)
                                                                                   ggTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCg                                                                      >  8:1710768/1‑149 (MQ=255)
                                                                                      tAACGACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGTTCGGTATCGTTCGGGTCGAgcg                                                                  <  5:877441/150‑1 (MQ=255)
                                                                                        aCCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCCTCTGGTCGGTATCGTTCGGGTCGAGCGcc                                                                >  2:929028/1‑150 (MQ=255)
                                                                                          cACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCAcc                                                             >  4:1501053/1‑151 (MQ=255)
                                                                                               tCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTg                                                        >  1:252887/1‑151 (MQ=255)
                                                                                                cAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGATCGCCACCGCCTgg                                                       <  8:324887/151‑1 (MQ=255)
                                                                                                    ggTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCAATCTGGTAGGTATCGTTCGGGTCGAGCGCCACCGCCGGGTGAc                                                   >  6:1635681/1‑151 (MQ=255)
                                                                                                    ggTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGAc                                                   <  5:147370/151‑1 (MQ=255)
                                                                                                      tACGACCATAAGTGCCAACATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGAcc                                                  >  4:591086/1‑150 (MQ=255)
                                                                                                        cGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAAt                                               <  1:1007291/151‑1 (MQ=255)
                                                                                                           ccATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGAt                                            <  4:1032198/151‑1 (MQ=255)
                                                                                                            cATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATc                                           >  7:1829963/1‑151 (MQ=255)
                                                                                                             aTAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGCGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTAGCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCg                                          >  6:1780018/1‑151 (MQ=255)
                                                                                                             aTAAGTGCCATCATAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCg                                          >  1:667851/1‑151 (MQ=255)
                                                                                                             aTAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCg                                          >  2:1704296/1‑151 (MQ=255)
                                                                                                                        catAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATcc                               >  6:963091/1‑151 (MQ=255)
                                                                                                                        catAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATc                                <  8:1769633/150‑1 (MQ=255)
                                                                                                                        catAAACACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATc                                <  1:1505133/150‑1 (MQ=255)
                                                                                                                         atAAACACGGTGAATACCAACCTGATACCCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGAGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGAGCAATGATCGCCTGATAATCCt                              >  6:1271250/1‑151 (MQ=255)
                                                                                                                           aaaCACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTgg                            >  3:209468/1‑151 (MQ=255)
                                                                                                                            nncacGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTGCGGGTCGAGCTCCCCCGCCTGGTGACCAATGATCGCCTGATAATCCTgg                            >  4:357763/3‑150 (MQ=255)
                                                                                                                            aaCACGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCAATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTgg                            >  3:472609/1‑150 (MQ=255)
                                                                                                                            aaCACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGa                           <  5:1669552/151‑1 (MQ=255)
                                                                                                                             agacGGTGAATACCATCGTGATACACCAGTTCAGTTGTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGGCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGaa                          <  2:1511958/149‑1 (MQ=255)
                                                                                                                             acacGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGa                           >  6:580432/1‑150 (MQ=255)
                                                                                                                               ccGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATATGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGt                        <  5:1285701/150‑1 (MQ=255)
                                                                                                                               acGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGt                        >  5:390540/1‑151 (MQ=255)
                                                                                                                                cGGTGAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTc                       <  1:1723531/151‑1 (MQ=255)
                                                                                                                                    gAATACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGt                    >  6:1147518/1‑150 (MQ=255)
                                                                                                                                    gAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGCCCAATGATCGCCTGATAATCCTGGAAGTCCGt                    >  4:976959/1‑150 (MQ=255)
                                                                                                                                     aaTACCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTaa                  <  5:1867629/151‑1 (MQ=255)
                                                                                                                                       tACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATg                >  7:1763104/1‑151 (MQ=255)
                                                                                                                                        aCCAACGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATg                <  7:72728/150‑1 (MQ=255)
                                                                                                                                         ccAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCg              <  2:1212640/151‑1 (MQ=255)
                                                                                                                                          cAACGTGATACACCAGTTCAGTTCTGCGCTAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCgg             <  3:33697/151‑1 (MQ=255)
                                                                                                                                          cAACGTGATAAGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCgg             <  1:127951/151‑1 (MQ=255)
                                                                                                                                           aaCGTGATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCgg             >  1:728453/1‑150 (MQ=255)
                                                                                                                                                gATACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCGGAAATCa       <  4:1886058/151‑1 (MQ=255)
                                                                                                                                                 aTACGCCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCGGAAATCAc      <  3:1268313/151‑1 (MQ=255)
                                                                                                                                                    cacCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCGGAAATCACGTa   <  1:1090546/151‑1 (MQ=255)
                                                                                                                                                     acCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCGGAAATCACGTaa  <  6:1384634/151‑1 (MQ=255)
                                                                                                                                                     acCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCGGAAATCACGTaa  <  3:64251/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CGATTTCAGGGAGGTCTGGATCAGGAAGAAAAGTAGATACAGCAGCATGGCTGGCAGCATCGACAGTACGCGTCCCTGACGTGGGTTAACCACGCTCAGCGGTACGACCATAAGTGCCATCATAAACACGGTGAATACCAACGTGATACACCAGTTCAGTTCTGCGCGAGCACGATCGGTGTCAGTGTTCCACAATGTGCGCATGTCCATCTGGTCGGTATCGTTCGGGTCGAGCGCCACCGCCTGGTGACCAATGATCGCCTGATAATCCTGGAAGTCCGTAATGCGGAAATCACGTAA  >  DH1/4026634‑4026933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑