Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,869,4740TC52.4% 60.8 / inf 478K523E (AAA→GAA) thrAbifunctional aspartate kinase/homoserine dehydrogenase I
Reads supporting (aligned to +/- strand):  ref base T (119/108);  new base C (125/125);  total (244/234)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-02

Only 100 of 496 total aligned reads displayed.

GAAACCTTCGCGCAGGAAGTCGGCATATTGATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCCAGCAGCGCACCGCCAACGCCACCG  >  DH1/3869325‑3869622
                                                                                                                                                     |                                                                                                                                                    
 aaaCCTTCGCGCAGGAAGTCGGCATATTGATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGttcttcct                                                                                                                                                    <  8:1184552/151‑1 (MQ=255)
  aaCCTTCGCGCAGGAAGTCGGCATATTGATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTg                                                                                                                                                   >  4:322737/1‑151 (MQ=255)
    ccTTCGCGCAGGAAGTCGGCATATTGATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGcc                                                                                                                                                 >  1:937857/1‑151 (MQ=255)
        cgcgCAGGAAGTCGGCATATTGATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGtt                                                                                                                                             <  4:1638297/151‑1 (MQ=255)
        cgcgCAGGAAGTCGGCATATTGATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGtt                                                                                                                                             >  3:1351364/1‑151 (MQ=255)
        cgcgCAGGAAGTCGGCATATTGATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGtt                                                                                                                                             <  8:1075137/151‑1 (MQ=255)
                 aGTCGGCATATTGATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGAtt                                                                                                                                    <  1:1337178/151‑1 (MQ=255)
                 aGTCGGCATATTGATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGAtt                                                                                                                                    <  4:1097454/151‑1 (MQ=255)
                 aGTCGGCATATTGATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGAtt                                                                                                                                    <  6:45998/151‑1 (MQ=255)
                  gTCGGCATATTGATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGAtt                                                                                                                                    >  4:772895/1‑150 (MQ=255)
                    cGGCATATTGATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTaa                                                                                                                                  <  3:511629/150‑1 (MQ=255)
                     ggCATATTGATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAgg                                                                                                                                >  6:113190/1‑151 (MQ=255)
                        atatTGATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGcc                                                                                                                              >  6:1235318/1‑150 (MQ=255)
                         tatTGATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCAt                                                                                                                            >  5:1859938/1‑151 (MQ=255)
                             gATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTa                                                                                                                         <  2:23788/150‑1 (MQ=255)
                              aTCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTAc                                                                                                                        >  4:1841533/1‑150 (MQ=255)
                                gcggcACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACAt                                                                                                                      <  1:625103/146‑1 (MQ=255)
                                ccGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACAtt                                                                                                                     >  8:724652/1‑151 (MQ=255)
                                 cGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTg                                                                                                                    <  1:379080/151‑1 (MQ=255)
                                      cTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCG‑CCGAGATTAAACCGCAAATTGGCATTCCGCAGTTGGTTAAGGCAAGTTTACAAAATAAGGCCATGTAAATTTGTTAGc                                                                                                              >  2:596248/1‑151 (MQ=255)
                                       tGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACAt                                                                                                                      >  5:148641/1‑143 (MQ=255)
                                        gCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGc                                                                                                              <  1:1821978/150‑1 (MQ=255)
                                         ccTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGc                                                                                                              >  7:388161/1‑149 (MQ=255)
                                         ccTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGc                                                                                                              >  5:257868/1‑149 (MQ=255)
                                         ccTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCag                                                                                                            <  2:681783/151‑1 (MQ=255)
                                               tggAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCtt                                                                                                      >  8:1503585/1‑151 (MQ=255)
                                                ggAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCtt                                                                                                      <  1:567544/150‑1 (MQ=255)
                                                ggAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTc                                                                                                     <  1:1614601/151‑1 (MQ=255)
                                                ggAAGTGCAGTCAACAATGACCGGGTTCAACAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCt                                                                                                       <  7:590215/149‑1 (MQ=255)
                                                 gAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCg                                                                                                    <  8:1361621/151‑1 (MQ=255)
                                                  aaGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCg                                                                                                    >  3:993903/1‑150 (MQ=255)
                                                   aGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCg                                                                                                    >  1:1161058/1‑149 (MQ=255)
                                                   aGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAg                                                                                                  <  5:390799/151‑1 (MQ=255)
                                                   aGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCg                                                                                                    >  5:62824/1‑149 (MQ=255)
                                                                aTGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGc                                                                                     >  2:1677425/1‑151 (MQ=255)
                                                                    ccGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGac                                                                                 >  3:1736158/1‑151 (MQ=255)
                                                                      gggTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGacac                                                                               <  4:1239731/151‑1 (MQ=255)
                                                                       ggTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACg                                                                              <  4:1881425/151‑1 (MQ=255)
                                                                         ttCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGt                                                                             >  2:355907/1‑150 (MQ=255)
                                                                          tCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTa                                                                            <  1:1093073/150‑1 (MQ=255)
                                                                           cagcagATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTaa                                                                           <  4:1773395/150‑1 (MQ=255)
                                                                           cagcagATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTa                                                                            >  8:1659029/1‑149 (MQ=255)
                                                                             gcagATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTc                                                                        <  2:1320777/151‑1 (MQ=255)
                                                                             gcagATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTc                                                                        <  8:1591920/151‑1 (MQ=255)
                                                                                gatgatATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGat                                                                     <  2:1747255/151‑1 (MQ=255)
                                                                                 atgatATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGat                                                                     >  1:1036482/1‑150 (MQ=255)
                                                                                     tatTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGtt                                                                >  6:334440/1‑151 (MQ=255)
                                                                                       ttCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCAAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACAACCCAGACACCTAAGTCGATATGttt                                                               >  1:1778123/1‑150 (MQ=255)
                                                                                         atttCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTAtt                                                            <  7:1506657/150‑1 (MQ=255)
                                                                                           ttCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTAtt                                                            <  3:660557/149‑1 (MQ=255)
                                                                                           ttCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTAt                                                             <  6:893708/148‑1 (MQ=255)
                                                                                             cACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTAttctt                                                         <  6:1455535/150‑1 (MQ=255)
                                                                                             cACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTGTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTAttctt                                                         <  1:1660325/150‑1 (MQ=255)
                                                                                               cGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCGAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGGAGAGCCTTCGAGTTGGGAACACCGCAGACACGTAAGTGGATATGTTTAttcttc                                                        >  2:1432933/1‑149 (MQ=255)
                                                                                                gAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTcagc                                                     >  3:247014/1‑151 (MQ=255)
                                                                                                gAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTcagc                                                     <  3:1535876/151‑1 (MQ=255)
                                                                                                gAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTcagc                                                     >  5:1157758/1‑151 (MQ=255)
                                                                                                 gggCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTGCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTcagcc                                                    <  4:66809/150‑1 (MQ=255)
                                                                                                 aGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTcagcc                                                    >  6:99876/1‑151 (MQ=255)
                                                                                                    cGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTcagccagc                                                 >  5:502891/1‑151 (MQ=255)
                                                                                                    cGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTcagccagc                                                 >  8:577201/1‑151 (MQ=255)
                                                                                                     gAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCt                                                >  5:1688195/1‑151 (MQ=255)
                                                                                                      aaTTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGGGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCtt                                               <  1:715516/151‑1 (MQ=255)
                                                                                                      aaTTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCtt                                               >  8:324611/1‑151 (MQ=255)
                                                                                                       attacccgcCCGAGATTAAACGGCTCTTTGGCTTGAGCCAGTTCTTTCTTCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGGttt                                              <  6:445162/145‑1 (MQ=255)
                                                                                                       aTTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCttt                                              >  7:1609249/1‑151 (MQ=255)
                                                                                                       aTTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCttt                                              <  5:1097002/151‑1 (MQ=255)
                                                                                                       aTTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCttt                                              >  1:788099/1‑151 (MQ=255)
                                                                                                        ttAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTg                                             >  8:486117/1‑151 (MQ=255)
                                                                                                           aGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTg                                          <  6:1769634/151‑1 (MQ=255)
                                                                                                           aGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTGAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTg                                          <  4:1348690/151‑1 (MQ=255)
                                                                                                           aGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCAAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTg                                          >  8:949668/1‑151 (MQ=255)
                                                                                                             cgcCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCTACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGAc                                        <  3:1813241/151‑1 (MQ=255)
                                                                                                              gcCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACg                                       >  5:1339040/1‑151 (MQ=255)
                                                                                                               cccGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGc                                      <  3:1418649/151‑1 (MQ=255)
                                                                                                               cccGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGc                                      >  3:1046697/1‑151 (MQ=255)
                                                                                                                 cGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCtt                                    <  6:1639820/151‑1 (MQ=255)
                                                                                                                 cGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCCGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCtt                                    <  5:777730/151‑1 (MQ=255)
                                                                                                                    gaTTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAg                                 <  2:782181/151‑1 (MQ=255)
                                                                                                                    gaTTAAACGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTAAATTGGTGAGCAGAGCCTTCGAGTTGTCAACACAGCAGACAAGTAAGTCGATATGTTTATTCTTCAGCAAGCTTTGCTGACGCTTc                                   >  1:1893707/1‑149 (MQ=255)
                                                                                                                     aTTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGt                                <  4:433653/151‑1 (MQ=255)
                                                                                                                        aaaCGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTg                              <  7:126033/150‑1 (MQ=255)
                                                                                                                          aCGGCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTc                           <  8:1880006/151‑1 (MQ=255)
                                                                                                                          aCGGCTCTTTGGCGTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTc                           <  7:844106/151‑1 (MQ=255)
                                                                                                                             gCTCTTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTcc                          >  7:1068119/1‑149 (MQ=255)
                                                                                                                               tctTTGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCcagc                       >  7:274966/1‑150 (MQ=255)
                                                                                                                                 tttGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCCagcagc                    >  2:1472710/1‑151 (MQ=255)
                                                                                                                                 tttGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTAGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCCagcagc                    >  6:636547/1‑151 (MQ=255)
                                                                                                                                 tttGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCCagcagc                    >  4:1486524/1‑151 (MQ=255)
                                                                                                                                  ttGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCCagcagc                    <  6:800144/150‑1 (MQ=255)
                                                                                                                                  ttGGCTTGCGCCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCCagcagc                    <  3:383429/150‑1 (MQ=255)
                                                                                                                                    ggCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCCAGCAGCGCa                 <  7:175936/151‑1 (MQ=255)
                                                                                                                                          cgcCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCCAGCAGCGCACCGCCa           <  8:1775852/151‑1 (MQ=255)
                                                                                                                                           gcCAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCCAGCAGCGCACCGCCa           >  4:882639/1‑150 (MQ=255)
                                                                                                                                            ccAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCCAGCAGCGCACCGCCAAc         <  2:979906/151‑1 (MQ=255)
                                                                                                                                            ccAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCCAGCAGCGCACCGCCAAc         <  5:904591/151‑1 (MQ=255)
                                                                                                                                            ccAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCCAGCAGCGCACCGCCAAc         <  7:1924022/151‑1 (MQ=255)
                                                                                                                                            ccAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCCAGCAGCGCACCGCCAAc         >  3:794096/1‑151 (MQ=255)
                                                                                                                                             cAGTTCTTCCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCCAGCAGCGCACCGCCAACg        >  2:473477/1‑151 (MQ=255)
                                                                                                                                                 tctttctGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCCAGCAGCGCACCGCCAACGCCAc    >  3:1231579/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAAACCTTCGCGCAGGAAGTCGGCATATTGATCCGCCACTGCCTGGCTGGAAGTGCAGTCAACAATGACCGGGTTCAGCAGATGATATTCTTTCACGAGGCGAATTAAGCGCCCGAGATTAAACGGCTCTTTGGCTTGCGCCAGTTCTTTCTGCCAGTTTTCCAGATTAAGGCCATGTACATTGGTGAGCAGAGCCTTCGAGTTGGCAACACCGCAGACACGTAAGTCGATATGTTTATTCTTCAGCCAGCTTTGCTGACGCTTCAGTTGCTCCAGCAGCGCACCGCCAACGCCACCG  >  DH1/3869325‑3869622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑