Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,559,2380TC56.4% 207.7 / inf 536intergenic (‑395/‑429)ykgL/ecpRhypothetical protein/helix‑turn‑helix transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (129/103);  new base C (171/129);  total (304/232)
Fisher's exact test for biased strand distribution p-value = 7.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.70e-04

Only 100 of 549 total aligned reads displayed.

ACGTACATACGCCCGTTCAATTTTCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGAAACCAAATGTCTTTTTCTTC  >  DH1/3559088‑3559387
                                                                                                                                                      |                                                                                                                                                     
  gTACATACGCCCGTTCAATTTTCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCttt                                                                                                                                                     >  1:934105/1‑151 (MQ=255)
     cATACGCCCGTTCAATTTTCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTaa                                                                                                                                                   >  3:509051/1‑150 (MQ=255)
     aaTACGCCCGTTCAATTTTCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTaaa                                                                                                                                                  <  4:1052485/150‑1 (MQ=255)
        aCGCCCGTTCAATTTTCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGCTTTATATTCTATCCCTTACCCTTAAAACt                                                                                                                                               <  1:1411753/151‑1 (MQ=255)
         cGCCCGTTCAATTTTCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACtt                                                                                                                                              <  6:324857/151‑1 (MQ=255)
          gCCCGTTCAATTTTCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTg                                                                                                                                             <  6:837335/151‑1 (MQ=255)
           cccGTTCAATTTTCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCAAAGTTTTATATTCTATCCCTTACCCTTAAAACTTGt                                                                                                                                            <  4:1339250/151‑1 (MQ=255)
               ttCAATTTTCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGttttt                                                                                                                                        >  5:554729/1‑151 (MQ=255)
                 cAATTTTCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGttttttt                                                                                                                                      <  1:1189523/151‑1 (MQ=255)
                 cAATTTTCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGtttttt                                                                                                                                       <  7:931857/150‑1 (MQ=255)
                 cAATTTTCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGttttttt                                                                                                                                      <  6:474442/151‑1 (MQ=255)
                 cAATTTGCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGttttttt                                                                                                                                      >  2:1139871/1‑151 (MQ=255)
                 cAATTCTCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGtttttt                                                                                                                                       <  6:1138914/150‑1 (MQ=255)
                  aaTTTTCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTa                                                                                                                                     <  5:217256/151‑1 (MQ=255)
                     tttCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACt                                                                                                                                   >  5:1257475/1‑150 (MQ=255)
                     tttCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACt                                                                                                                                   <  4:1762191/150‑1 (MQ=255)
                     tttCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACt                                                                                                                                   >  1:831407/1‑150 (MQ=255)
                      ttCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTa                                                                                                                                  <  7:1262787/150‑1 (MQ=255)
                      ttCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATAAATTAAAATATCTTACTGATTGGCAAATCATATTCAATGACAGCTAATCATAGTTTTATATTCAATCCCTTACACTTAAAACTAATTTTATTAATat                                                                                                                                 >  2:1858802/1‑150 (MQ=255)
                        ccATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTc                                                                                                                               <  7:54299/151‑1 (MQ=255)
                            tACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATc                                                                                                                           >  8:776890/1‑151 (MQ=255)
                             aCTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATc                                                                                                                           >  8:1112190/1‑150 (MQ=255)
                             aCTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATc                                                                                                                           >  7:893579/1‑150 (MQ=255)
                             aCTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATc                                                                                                                           >  3:206951/1‑150 (MQ=255)
                              cTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATcac                                                                                                                         >  3:1714239/1‑151 (MQ=255)
                              cTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCAtaa                                                                                                                          >  6:1250417/1‑148 (MQ=255)
                                tATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATcaca                                                                                                                        <  8:303699/150‑1 (MQ=255)
                                 aTTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATcacac                                                                                                                       >  7:1229755/1‑150 (MQ=255)
                                   tGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAgc                                                                                                                    >  7:644652/1‑151 (MQ=255)
                                   tGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAgc                                                                                                                    >  7:1076166/1‑151 (MQ=255)
                                     gAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAgcgc                                                                                                                  >  2:432808/1‑151 (MQ=255)
                                      aaTGAACACATGTAACCATTTTGATCGGCATGTTAATCCAATAAAATATCTTACTGATTGGAAAAAAATCTTCAATGACAGCTCATCATAGTATTATATTCTATCACTTACCCTTAAAACTTGTATTTATACTAGTCCATCACACAgagc                                                                                                                  >  8:1830702/1‑150 (MQ=255)
                                           acacCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGATAGCTCATCATAGATTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTAATAGTCAATCACACAGCGCATTa                                                                                                              >  6:204017/1‑149 (MQ=255)
                                           acacCTGTAACCATTTTCTGCCGCATGTTAATCAATTAAAATATCTTACTCATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTAATATTCTATCCCTTACCTTAAAAACTAGTTTATTTAATAGTCCATCAAACAGCGCATTaat                                                                                                            >  8:839801/1‑150 (MQ=255)
                                              ccTGTAAGCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACt                                                                                                         <  2:679068/151‑1 (MQ=255)
                                               cTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACt                                                                                                         >  6:1427520/1‑150 (MQ=255)
                                                tGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTAt                                                                                                       >  8:385211/1‑151 (MQ=255)
                                                 gTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTAtt                                                                                                      >  7:33362/1‑151 (MQ=255)
                                                    aCCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCt                                                                                                   <  2:1674213/151‑1 (MQ=255)
                                                     ccATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCt                                                                                                   >  6:1688234/1‑150 (MQ=255)
                                                      cATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTa                                                                                                  >  5:748931/1‑150 (MQ=255)
                                                          ttGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAacac                                                                                              <  8:1683790/150‑1 (MQ=255)
                                                           tgtgCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACtt                                                                                            >  7:69522/1‑151 (MQ=255)
                                                            gtgCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTc                                                                                           >  3:1490172/1‑151 (MQ=255)
                                                            gtgCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACtt                                                                                            >  4:491701/1‑150 (MQ=255)
                                                             tgCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTc                                                                                           >  1:491816/1‑150 (MQ=255)
                                                               cGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAgg                                                                                        >  3:1817111/1‑151 (MQ=255)
                                                                   aTGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCa                                                                                     >  3:284106/1‑150 (MQ=255)
                                                                      ttAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGt                                                                                 >  7:153378/1‑151 (MQ=255)
                                                                        aaTCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGtt                                                                                <  3:977216/150‑1 (MQ=255)
                                                                        aaTCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTc                                                                               >  4:886177/1‑151 (MQ=255)
                                                                              ttAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGAcc                                                                         >  7:1831441/1‑151 (MQ=255)
                                                                                aaaaTATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATATTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGAGCa                                                                        <  7:1114563/150‑1 (MQ=255)
                                                                                aaaaTATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCa                                                                        >  6:979919/1‑150 (MQ=255)
                                                                                 aaaTATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAat                                                                      >  5:300669/1‑151 (MQ=255)
                                                                                 aaaTATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAat                                                                      >  5:411862/1‑151 (MQ=255)
                                                                                   atatCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAatat                                                                    >  1:131483/1‑151 (MQ=255)
                                                                                   atatCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAatat                                                                    <  6:686937/151‑1 (MQ=255)
                                                                                     atCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAAtata                                                                   >  1:725376/1‑150 (MQ=255)
                                                                                         tACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATaa                                                                  <  7:1390698/147‑1 (MQ=255)
                                                                                         tACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATg                                                              >  7:566892/1‑151 (MQ=255)
                                                                                            tGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCa                                                            >  3:1348387/1‑150 (MQ=255)
                                                                                            tGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCa                                                            >  5:1866168/1‑150 (MQ=255)
                                                                                             gATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAg                                                          <  2:861383/151‑1 (MQ=255)
                                                                                             gATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAg                                                          <  3:1605349/151‑1 (MQ=255)
                                                                                                  gCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTa                                                        >  5:1635369/1‑148 (MQ=255)
                                                                                                   gaatttattttaaaTGACAGGTTATTATTGTTTTATTTTTTATGTCTTAGCTTTATAACTTGTTTTTTTTGTTGTCCATGAGTCAGCGGATTAAGACTTTTCCTAAGACTTCTGGGCTAAGTTCCTGACCAATATAAAATGGAAGTAAGa                                                     <  2:353464/138‑1 (MQ=255)
                                                                                                   cAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGaa                                                    <  5:1037036/151‑1 (MQ=255)
                                                                                                    aaaTCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAAt                                                   >  8:1318711/1‑151 (MQ=255)
                                                                                                    aaaTCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAAt                                                   >  4:565990/1‑151 (MQ=255)
                                                                                                       tcatcTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTg                                                 >  6:1742457/1‑150 (MQ=255)
                                                                                                          tcTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGAAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACg                                             >  4:1490209/1‑151 (MQ=255)
                                                                                                           attCAATGACATCTCATCATAGTTTTATATTCTATCACTTACATTTAAAACTTGTTTTTTTACTATTCCATCACAAAGCGAATTAAGACTATTACTAACACTTAAGGGCAAAGTTCCTGAACAATATAAAATCCAAGTAAGAATTGAACGt                                            >  3:783943/2‑151 (MQ=255)
                                                                                                            ttCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGtt                                           >  1:1031118/1‑151 (MQ=255)
                                                                                                              cAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTtat                                         <  2:1532126/151‑1 (MQ=255)
                                                                                                               aaTGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTATTACATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTtata                                        <  1:1570225/151‑1 (MQ=255)
                                                                                                                 tGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATAtt                                      >  7:200617/1‑151 (MQ=255)
                                                                                                                   aCAGCTCATCATAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGc                                    <  5:1351880/151‑1 (MQ=255)
                                                                                                                         catcatAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATaa                              <  3:634105/151‑1 (MQ=255)
                                                                                                                         catcatAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATaa                              <  1:299376/151‑1 (MQ=255)
                                                                                                                         catcatAGTTTTATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGCAAGAATTGAACGTTATATTGCCAATaa                              <  5:1752255/151‑1 (MQ=255)
                                                                                                                               aGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAAGGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTAt                        <  7:1668778/151‑1 (MQ=255)
                                                                                                                               aGTTTAATATTCTATCCCTTACCCTTAAAACTTGTTTATTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTAt                        <  4:1220573/151‑1 (MQ=255)
                                                                                                                                gTTTTATATTCTCTCCCTTCCCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATg                       <  3:863365/151‑1 (MQ=255)
                                                                                                                                 ttttATATTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATg                       >  8:620939/1‑150 (MQ=255)
                                                                                                                                   ttATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGaa                     <  1:1513226/150‑1 (MQ=255)
                                                                                                                                    tataTTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGGGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGaaa                    <  3:1205961/150‑1 (MQ=255)
                                                                                                                                      tatTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGAAAcc                  >  7:998656/1‑150 (MQ=255)
                                                                                                                                       atTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGAAACCa                 >  4:798543/1‑150 (MQ=255)
                                                                                                                                       atTCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGAAACCa                 >  2:981176/1‑150 (MQ=255)
                                                                                                                                        ttCTATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGAAACCaa                >  2:588625/1‑150 (MQ=255)
                                                                                                                                          atATCCCTTAACTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGAAACCAAATg             <  2:120173/150‑1 (MQ=255)
                                                                                                                                           tATCCCTTACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGAAACCAAATGt            >  8:1486277/1‑151 (MQ=255)
                                                                                                                                             tCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGAAACCAAATGTCt          >  2:1481516/1‑151 (MQ=255)
                                                                                                                                              cccttacccttaAAACTTGTTTTTTTACTAGCCCATCACACAGCTCATTAAGAATATTCCTAACACTTCAGGTCAAAGTTCCTGACCAATATAAAATGAAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGAAACCAAATGTCt          <  8:672535/150‑1 (MQ=255)
                                                                                                                                               ccTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGAAACCAAATGTCttt        >  1:361026/1‑151 (MQ=255)
                                                                                                                                                  tACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGAAACCAAATGTCtttttg     >  4:1554690/1‑150 (MQ=255)
                                                                                                                                                  tACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGAAACCAAATGTCtttt       <  5:1541368/149‑1 (MQ=255)
                                                                                                                                                  tACCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGAAACCAAATGTCtttt       <  4:331934/149‑1 (MQ=255)
                                                                                                                                                   aCCCTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGAAACCAAATGTCTTTttc     <  1:1804741/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ACGTACATACGCCCGTTCAATTTTCCATTACTTATTGGAATGAACACCTGTAACCATTTTGTGCGGCATGTTAATCCATTAAAATATCTTACTGATTGGCAAATCATCTTCAATGACAGCTCATCATAGTTTTATATTCTATCCCTTACCTTTAAAACTTGTTTTTTTACTAGTCCATCACACAGCGCATTAAGACTATTCCTAACACTTCAGGGCAAAGTTCCTGACCAATATAAAATGCAAGTAAGAATTGAACGTTATATTGCCAATAACCTTATGAAACCAAATGTCTTTTTCTTC  >  DH1/3559088‑3559387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑