BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	2,732,543	0	A	G	52.4%	72.0 / inf	495	S135P (TCG→CCG)	yceD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (94/141); new base G (124/135); total (219/276)
Fisher's exact test for biased strand distribution p-value = 8.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.22e-01

Only 100 of 506 total aligned reads displayed.

GGTTTATCCCCGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACGTTGTGTA  >  DH1/2732393‑2732693

 gTTTATCCCCGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGaa                                                                                                                                                       <  6:892716/151‑1 (MQ=255)

    tATCCCCGATCCAACGGGGAGCACCAATTACTTACTCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGAGCTTCTTCAGGCAGTTCAGCATAGACCATGTCCGCTTCGGACAATTCACAGTGTTCAGAATCATGCACCGGAAc                                                                                                                                                     <  6:721894/150‑1 (MQ=255)

     aTCCCCGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACt                                                                                                                                                    >  2:234293/1‑150 (MQ=255)

        cccGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCg                                                                                                                                                >  7:619066/1‑151 (MQ=255)

          cGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGc                                                                                                                                              >  6:374636/1‑151 (MQ=255)

          cGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGc                                                                                                                                              <  2:1137292/151‑1 (MQ=255)

           gATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGc                                                                                                                                              >  4:719433/1‑150 (MQ=255)

            aTCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCa                                                                                                                                             >  1:721917/1‑150 (MQ=255)

             tCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCa                                                                                                                                             >  6:1295189/1‑149 (MQ=255)

              ccAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAgg                                                                                                                                          <  4:1229117/151‑1 (MQ=255)

               cAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGc                                                                                                                                         >  1:957968/1‑151 (MQ=255)

                   ggggAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAgg                                                                                                                                     <  6:1606993/151‑1 (MQ=255)

                         cACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGAtt                                                                                                                               <  8:75860/151‑1 (MQ=255)

                         cACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGAtt                                                                                                                               <  4:1548010/151‑1 (MQ=255)

                          aCCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGAttt                                                                                                                              >  1:488156/1‑151 (MQ=255)

                          aCCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGAttt                                                                                                                              <  1:1836735/151‑1 (MQ=255)

                           ccAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTc                                                                                                                             >  5:432861/1‑151 (MQ=255)

                           ccAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTc                                                                                                                             <  5:300445/151‑1 (MQ=255)

                            cAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGAAGATTTc                                                                                                                             >  7:784939/1‑150 (MQ=255)

                             aaTTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCTCCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCAt                                                                                                                           >  8:522443/1‑151 (MQ=255)

                             aaTTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCAt                                                                                                                           >  5:78929/1‑151 (MQ=255)

                              aTTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATc                                                                                                                          >  3:559258/1‑151 (MQ=255)

                              aTTACTTACGCGTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATc                                                                                                                          <  2:1365351/151‑1 (MQ=255)

                               ttacttacGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCt                                                                                                                         >  3:140354/1‑151 (MQ=255)

                                 acatacTCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTc                                                                                                                       <  5:1037417/148‑1 (MQ=255)

                                    tacGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAAc                                                                                                                    >  1:79722/1‑151 (MQ=255)

                                        cTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCAtt                                                                                                                >  1:420334/1‑151 (MQ=255)

                                         tttAAGCTCGCTAATACCGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGAACAGTAAATACAGGCAAGCCGAGCATCAATTCAACTTCAACCAt                                                                                                                 >  2:924608/1‑149 (MQ=255)

                                          ttAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCAtt                                                                                                                <  1:1116847/149‑1 (MQ=255)

                                          ttAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTg                                                                                                               <  8:1655243/150‑1 (MQ=255)

                                           tAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGc                                                                                                              <  3:1033706/150‑1 (MQ=255)

                                           tAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGc                                                                                                              <  5:1534878/150‑1 (MQ=255)

                                            aaGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCa                                                                                                             <  7:936550/150‑1 (MQ=255)

                                                 cgCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGc                                                                                                          >  7:1813751/2‑148 (MQ=255)

                                                  gCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGc                                                                                                          >  3:1687393/1‑147 (MQ=255)

                                                     aaTACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCg                                                                                                    <  5:215713/150‑1 (MQ=255)

                                                        aCGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGAttt                                                                                                >  8:1780329/1‑151 (MQ=255)

                                                          ggCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCa                                                                                              <  6:1094748/151‑1 (MQ=255)

                                                            cAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCAcc                                                                                            >  3:820787/1‑151 (MQ=255)

                                                               aTGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGa                                                                                          >  1:1341541/1‑150 (MQ=255)

                                                                tGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAAt                                                                                        >  5:1756397/1‑151 (MQ=255)

                                                                 ggggTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAAt                                                                                        <  2:803687/150‑1 (MQ=255)

                                                                 gggTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAAt                                                                                        <  3:957269/150‑1 (MQ=255)

                                                                  gggttGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTc                                                                                      <  2:588750/148‑1 (MQ=255)

                                                                   gTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTc                                                                                               >  2:1307003/1‑141 (MQ=255)

                                                                   gTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCg                                                                                     >  4:1723711/1‑151 (MQ=255)

                                                                    tttGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGt                                                                                    >  7:1741003/1‑151 (MQ=255)

                                                                     ttGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGt                                                                                    <  7:501674/150‑1 (MQ=255)

                                                                         cTTTTGCGCTTCTTCAGGCAGATCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAAcc                                                                               >  6:1416495/1‑151 (MQ=255)

                                                                          ttttGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCt                                                                              >  4:405208/1‑151 (MQ=255)

                                                                            ttGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTc                                                                             <  3:1853139/150‑1 (MQ=255)

                                                                                    cttcAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTc                                                                    >  4:856884/1‑151 (MQ=255)

                                                                                      tcAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTc                                                                    <  8:684244/149‑1 (MQ=255)

                                                                                       cAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCAt                                                                  >  7:199328/1‑150 (MQ=255)

                                                                                        aGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATAc                                                                <  7:1603246/151‑1 (MQ=255)

                                                                                          gCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGc                                                              <  2:1475744/151‑1 (MQ=255)

                                                                                          gCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTCTTCAGAAACATGCACCGAAACTACCGGCAAGGCCAGGATGATTTCATCTTCAACCATAGCAACCAGATCGATTTCACCGAATTCGTTAACCTCAAACGGTTCATACGc                                                              >  6:19450/1‑151 (MQ=255)

                                                                                                cACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCg                                                         <  2:713520/150‑1 (MQ=255)

                                                                                                cACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATCATTTCATCTTCAACCATTGCAAGCAGAACGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCttaccg                                                        >  5:67817/1‑147 (MQ=255)

                                                                                                 aCCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGc                                                       >  6:1846574/1‑151 (MQ=255)

                                                                                                  ccAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCa                                                      <  8:235484/151‑1 (MQ=255)

                                                                                                     aaGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTg                                                   >  1:1022408/1‑151 (MQ=255)

                                                                                                      aGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGc                                                  >  8:384634/1‑151 (MQ=255)

                                                                                                        aCCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCtt                                                >  7:1196548/1‑151 (MQ=255)

                                                                                                          cATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTc                                               >  8:839480/1‑150 (MQ=255)

                                                                                                          cATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCa                                              <  1:218265/151‑1 (MQ=255)

                                                                                                             gTCCGCTGCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGcc                                           <  3:1253352/151‑1 (MQ=255)

                                                                                                                 gCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGtt                                       <  3:327045/151‑1 (MQ=255)

                                                                                                                 gCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTACACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGtt                                       <  8:1897779/151‑1 (MQ=255)

                                                                                                                  cTTCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTc                                      >  1:1650341/1‑151 (MQ=255)

                                                                                                                   ttCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCg                                     >  2:175211/1‑151 (MQ=255)

                                                                                                                    tCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTc                                      <  8:1316991/149‑1 (MQ=255)

                                                                                                                    tCGGACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCg                                     <  3:1541286/150‑1 (MQ=255)

                                                                                                                      ggACACTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCt                                  <  8:1099632/151‑1 (MQ=255)

                                                                                                                      ggACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCt                                  <  6:1217380/151‑1 (MQ=255)

                                                                                                                        acacTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTg                                 >  3:619916/1‑150 (MQ=255)

                                                                                                                        acacTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTg                                 >  8:155057/1‑150 (MQ=255)

                                                                                                                          acTTCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAAc                              >  2:1235828/1‑151 (MQ=255)

                                                                                                                             tCACAGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGc                            <  2:166500/150‑1 (MQ=255)

                                                                                                                             tCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGc                            <  5:1336988/150‑1 (MQ=255)

                                                                                                                               acaGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGcac                          >  8:1430134/1‑150 (MQ=255)

                                                                                                                               acaGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGcac                          >  2:100246/1‑150 (MQ=255)

                                                                                                                                caGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAg                        <  7:1524798/151‑1 (MQ=255)

                                                                                                                                caGTGTTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAg                        <  3:741117/151‑1 (MQ=255)

                                                                                                                                  gtgtTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGa                      <  7:337609/151‑1 (MQ=255)

                                                                                                                                    gtTCAGAATCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGAc                     <  8:1038827/150‑1 (MQ=255)

                                                                                                                                       cAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTaaa                 <  5:1256858/151‑1 (MQ=255)

                                                                                                                                         caaTCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAAc               <  6:1721577/150‑1 (MQ=255)

                                                                                                                                          aaTCATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAAc               >  4:1382097/1‑150 (MQ=255)

                                                                                                                                             cATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAACCAAt            >  5:1599717/1‑150 (MQ=255)

                                                                                                                                             cATGCACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATa           <  7:1412965/151‑1 (MQ=255)

                                                                                                                                             cATGCACCCGAACTACCGGCACGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATa           <  2:1040555/151‑1 (MQ=255)

                                                                                                                                                gCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACg         <  7:1068019/150‑1 (MQ=255)

                                                                                                                                                gCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACg         <  2:328882/150‑1 (MQ=255)

                                                                                                                                                gCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACAGAATTCGTTAATCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACGt        >  3:1790865/1‑151 (MQ=255)

                                                                                                                                                 cACCGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACGtt       <  2:431714/151‑1 (MQ=255)

                                                                                                                                                   ccGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACGTtgt     <  4:310610/151‑1 (MQ=255)

                                                                                                                                                   ccGGAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACGTtgt     >  5:931798/1‑151 (MQ=255)

                                                                                                                                                    cGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACGTtgtg    <  4:1125335/151‑1 (MQ=255)

                                                                                                                                                      aaaCTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACGTTGTGTa  <  4:1869524/151‑1 (MQ=255)

GGTTTATCCCCGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTAATACGGCAAATGGGTTTGGCTTTTGCGCTTCTTCAGGCAGTTCACCAAAGACCATGTCCGCTTCGGACACTTCACAGTGTTCAGAATCATGCACCGAAACTACCGGCAAGGCGAGGATGATTTCATCTTCAACCATTGCAAGCAGATCGATTTCACCGAATTCGTTAACCTCAATCGGTTCATACGCTTCCGGCAGTGCTTCAGCCTGTTCGTCTGAACGCACAGGACTAAAACAATACGTTGTGTA  >  DH1/2732393‑2732693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`