Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 3,668,924 (T)5→6 pseudogene (353/455 nt) lpxA ← hydroxymyristoyl‑ACP dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,668,9191.T96.7% 1578.0 / 35.1 452pseudogene (358/455 nt)lpxAhydroxymyristoyl‑ACP dehydratase
Reads supporting (aligned to +/- strand):  ref base . (11/4);  new base T (191/242);  total (206/246)
Fisher's exact test for biased strand distribution p-value = 3.34e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 489 total aligned reads displayed.

CCCTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCTGT  >  DH1/3668769‑3669064
                                                                                                                                                        |                                                                                                                                                  
 ccTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGcgcg                                                                                                                                                     >  6:35683/1‑150 (MQ=255)
 ccTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGt                                                                                                                                                   >  3:990491/1‑151 (MQ=255)
       ccACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGttttttc                                                                                                                                            >  5:152720/1‑149 (MQ=255)
       ccACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGtttttt                                                                                                                                             <  1:277807/150‑2 (MQ=255)
       ccACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGtttttt                                                                                                                                             <  3:1621101/150‑2 (MQ=255)
          caATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGttttttcgaa                                                                                                                                         <  6:1460112/151‑6 (MQ=255)
          caATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGttttttcga                                                                                                                                          >  1:138974/1‑146 (MQ=255)
           aaTGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGaaa                                                                                                                                         <  5:391495/151‑1 (MQ=255)
               gCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGCCCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTgc                                                                                                                                     >  7:339878/1‑150 (MQ=255)
               gCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTg                                                                                                                                      >  5:943560/1‑150 (MQ=255)
               gCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGa                                                                                                                                     <  5:827841/151‑1 (MQ=255)
                cGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGGTTTTTCGAAAGTGAc                                                                                                                                    >  8:874209/1‑151 (MQ=255)
                    tGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTcc                                                                                                                                >  7:70398/1‑151 (MQ=255)
                      gATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCAt                                                                                                                              <  3:1747740/151‑1 (MQ=255)
                        tGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATg                                                                                                                             >  5:1567573/1‑150 (MQ=255)
                         gCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGa                                                                                                                            <  6:499568/150‑1 (MQ=255)
                         gCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGAt                                                                                                                           >  4:204552/1‑151 (MQ=255)
                         gCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGAt                                                                                                                           <  4:1595109/151‑1 (MQ=255)
                         gCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGAt                                                                                                                           <  2:828633/151‑1 (MQ=255)
                         gCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGAt                                                                                                                           <  3:1072322/151‑1 (MQ=255)
                              aaGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCAttt                                                                                                                      <  8:631523/151‑1 (MQ=255)
                              aaGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCAttt                                                                                                                      <  5:1142044/151‑1 (MQ=255)
                              aaGGCGGATTTATCAATCACGTATCAGGCCTACCGTCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCAttt                                                                                                                      <  5:510670/151‑1 (MQ=255)
                               aGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTg                                                                                                                     >  7:57137/1‑151 (MQ=255)
                                ggCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTg                                                                                                                     >  4:1423217/1‑150 (MQ=255)
                                ggCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGa                                                                                                                    <  5:305313/151‑1 (MQ=255)
                                 gCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGAt                                                                                                                   >  1:444036/1‑151 (MQ=255)
                                  cGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATc                                                                                                                  >  3:1250390/1‑151 (MQ=255)
                                   ggATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGAGTTTTCGAAAGTGACTTCCATGATCATTTGATcc                                                                                                                 >  6:1742385/1‑150 (MQ=255)
                                     aTTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGGTTTTTGGAAAGTGCCTTCCATGATCATTTTTTCGcc                                                                                                               >  7:1094377/1‑151 (MQ=255)
                                       ttATCAATCACGTATCAGGGCTCCCGGCTACGAGCACACATCATCGTTTCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCa                                                                                                              <  8:1017470/150‑1 (MQ=255)
                                       ttATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGcc                                                                                                               >  8:352322/1‑149 (MQ=255)
                                         atcattcaCGTATCAGGGCTCGCGGCTACGAGCACACATCATCGTTGCTTCGCATACTACTTTAGGATCGACCAGATGAACCCCTTTAAAAGGGGTCAGGCCGCGGCGCGTTTTTTGGAAAGTGACTTCCATGATCTTTTGATCGCCAGGc                                                                                                           <  3:229295/151‑1 (MQ=255)
                                         atcaatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGc                                                                                                           <  8:1276369/151‑1 (MQ=255)
                                           caatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGGCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCAc                                                                                                         <  7:143323/151‑1 (MQ=255)
                                                 cGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCgg                                                                                                   >  3:1485943/1‑151 (MQ=255)
                                                      cAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCATCCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCt                                                                                              >  8:1877755/1‑151 (MQ=255)
                                                      cAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCt                                                                                              >  3:469512/1‑151 (MQ=255)
                                                       aGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCtt                                                                                             <  3:1881923/151‑1 (MQ=255)
                                                        ggCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTg                                                                                            >  6:438683/1‑151 (MQ=255)
                                                         gCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTg                                                                                            >  8:141034/1‑150 (MQ=255)
                                                         gCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGa                                                                                           <  5:1813572/151‑1 (MQ=255)
                                                           cTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGCCTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGa                                                                                           >  6:931052/1‑149 (MQ=255)
                                                           cTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTGTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGa                                                                                           >  2:555211/1‑149 (MQ=255)
                                                            tCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgc                                                                                        >  8:1155893/1‑151 (MQ=255)
                                                            tCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgc                                                                                        >  6:1100741/1‑151 (MQ=255)
                                                             cccGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAg                                                                                         <  1:847251/149‑1 (MQ=255)
                                                              ccGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcgc                                                                                      >  2:297862/1‑151 (MQ=255)
                                                              ccGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcgc                                                                                      >  2:1720088/1‑151 (MQ=255)
                                                              ccGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcgc                                                                                      <  3:897617/151‑1 (MQ=255)
                                                               ccgCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcgcg                                                                                     <  5:1462923/149‑1 (MQ=255)
                                                                ggCTACGAGCACACATAATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcgcgc                                                                                    <  8:42804/151‑1 (MQ=255)
                                                                   tACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTc                                                                                 >  4:471547/1‑151 (MQ=255)
                                                                   tACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTc                                                                                 >  2:1820182/1‑151 (MQ=255)
                                                                      gAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTc                                                                              >  5:1002368/1‑151 (MQ=255)
                                                                       aGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTc                                                                              >  3:758536/1‑150 (MQ=255)
                                                                         cacacaTCATCGTTGCTTCGCAAACTACTTTACCATCGCCCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAAt                                                                           <  7:228698/151‑1 (MQ=255)
                                                                         cacacaTCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGCGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAAt                                                                           <  5:1346554/151‑1 (MQ=255)
                                                                          acacaTCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTCACTTCCATGATCATTTGATCGCCAGGCCCGCCCGGGCCCTTGAAGCGCGCTTCCTCAAt                                                                           >  1:1637264/1‑150 (MQ=255)
                                                                               tcatcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGc                                                                     >  4:258546/1‑151 (MQ=255)
                                                                                catcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCGTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCg                                                                    <  5:657590/151‑1 (MQ=255)
                                                                                catcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCCGGCACGCCCGGGCGCTTGAAGCGCGCTTCGTCAATACCCGcc                                                                    >  2:932228/1‑150 (MQ=255)
                                                                                catcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCg                                                                    >  1:1805762/1‑151 (MQ=255)
                                                                                 atcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGa                                                                   <  4:1174670/151‑1 (MQ=255)
                                                                                   cGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGCCCGCTTGAAGCGCGCTTCGTCAATACCAGCGaat                                                                 >  2:1352893/1‑150 (MQ=255)
                                                                                       gCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagtagt                                                             <  2:1613968/151‑1 (MQ=255)
                                                                                         ttCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTAc                                                           >  5:1775700/1‑151 (MQ=255)
                                                                                          tctctaaCTAGTTTACAATCGACCTGTGCATCCCTTTTAAAACGGTTCTTGCTTCGGGGCGTTTTTTCGAAAGTGACTTCCATGATCATTTTATCGCCAGGCACGAGCGGGTGCTTGAAGCGCGCTTCGTAAATACCAGGTAAgtagta                                                            <  6:1544743/144‑1 (MQ=255)
                                                                                            gCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGAACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACCGc                                                        >  1:964160/1‑151 (MQ=255)
                                                                                               aaCTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCa                                                     <  6:1356199/151‑1 (MQ=255)
                                                                                                actactTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCAc                                                    <  8:1538838/151‑1 (MQ=255)
                                                                                                actactTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCAc                                                    <  2:648135/151‑1 (MQ=255)
                                                                                                  tactTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCAccc                                                  >  2:431043/1‑151 (MQ=255)
                                                                                                   actTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCg                                                 >  2:1677792/1‑151 (MQ=255)
                                                                                                       tACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTc                                             >  4:1131998/1‑151 (MQ=255)
                                                                                                         ccATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCa                                           <  4:331522/151‑1 (MQ=255)
                                                                                                             cGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGtt                                        <  1:1191870/150‑1 (MQ=255)
                                                                                                              gACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGtttt                                      <  5:792954/151‑1 (MQ=255)
                                                                                                               aCCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTc                                     >  1:558093/1‑151 (MQ=255)
                                                                                                                   gagCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTAc                                 >  8:1648143/1‑151 (MQ=255)
                                                                                                                      cAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCt                              <  4:909706/151‑1 (MQ=255)
                                                                                                                          cccTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTa                          <  1:1273199/151‑1 (MQ=255)
                                                                                                                           ccTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTaa                         <  4:576015/151‑1 (MQ=255)
                                                                                                                              ttAAATCGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGGGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTaaa                        <  5:988518/149‑1 (MQ=255)
                                                                                                                              ttAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAAc                       <  3:612904/150‑1 (MQ=255)
                                                                                                                              ttAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAAc                       <  2:1118422/150‑1 (MQ=255)
                                                                                                                               tAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCCCGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTGCTACGCTTTTAAACGc                     >  7:483601/1‑151 (MQ=255)
                                                                                                                               tAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACg                      <  6:197736/150‑1 (MQ=255)
                                                                                                                                  aaCGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGccgg                  <  1:1504927/151‑3 (MQ=255)
                                                                                                                                   aCGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGa                 <  6:797932/151‑1 (MQ=255)
                                                                                                                                   aCGGGGCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCCTTTGATCGCCACGCACGACCGGGCGGTTGCAGCGCGGTTCGGCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGa                 <  4:1685914/151‑1 (MQ=255)
                                                                                                                                       gTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATAc             <  5:1920956/151‑1 (MQ=255)
                                                                                                                                       gTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATAc             <  1:1042606/151‑1 (MQ=255)
                                                                                                                                        tCAGGCCGCGGCGCGGTTTTTCGAAAGTGACTTCCATGATCATTTGATAGCCAGGCACCACCGGGCCCTTGAAGCGCGCTTCGTCAAAACCAGCCAAGTAGTACAGCGCACCCGGTTGCAGTTTTACTAAGCTTTTAAACGCCAGAATAcc            >  7:1251883/1‑151 (MQ=255)
                                                                                                                                         cAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCt           <  7:870332/151‑1 (MQ=255)
                                                                                                                                            gCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACACTTTTAAACGCCAGAATACCTGtt        <  6:1271505/151‑1 (MQ=255)
                                                                                                                                            gCCGAGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTGAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGtt        <  7:1511063/151‑1 (MQ=255)
                                                                                                                                             ccGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTg       >  7:1650892/1‑151 (MQ=255)
                                                                                                                                             ccGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGCGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTg       <  6:587359/151‑1 (MQ=255)
                                                                                                                                                  gcgcGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCtgt  >  1:1852069/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                  
CCCTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCTGT  >  DH1/3668769‑3669064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: