Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 2,730,866 T→G I39L (ATC→CTC)  fabH ← 3‑oxoacyl‑ACP synthase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,730,8660TG97.7% 1502.2 / 6.1 391I39L (ATC→CTC) fabH3‑oxoacyl‑ACP synthase III
Reads supporting (aligned to +/- strand):  ref base T (3/6);  new base G (177/203);  total (182/209)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 391 total aligned reads displayed.

GGGAAAGCGTGCGTAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGATACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTTATATACCGTC  >  DH1/2730718‑2731013
                                                                                                                                                    |                                                                                                                                                   
  gAAAGCGTGCGTAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGGTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCg                                                                                                                                                 <  7:1624917/151‑1 (MQ=255)
   aaaGCGTGCGTAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCgg                                                                                                                                                >  3:1641338/1‑151 (MQ=255)
    aaGCGTGCGTAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGCCCGGt                                                                                                                                               >  6:420292/1‑151 (MQ=255)
        gtgcgtAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACg                                                                                                                                            >  5:1105208/1‑150 (MQ=255)
        gtgcgtAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACg                                                                                                                                            >  2:386597/1‑150 (MQ=255)
             tAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGAc                                                                                                                                      >  2:49150/1‑151 (MQ=255)
             tAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGAc                                                                                                                                      >  7:1565046/1‑151 (MQ=255)
              aGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACa                                                                                                                                     <  8:1616415/151‑1 (MQ=255)
               gCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACaa                                                                                                                                    <  6:1676463/151‑1 (MQ=255)
                cAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAAt                                                                                                                                   >  8:1285034/1‑151 (MQ=255)
                 aGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATc                                                                                                                                  >  7:147464/1‑151 (MQ=255)
                       cGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTc                                                                                                                             <  3:1177658/150‑1 (MQ=255)
                          tGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGTCAATCCACTGGTc                                                                                                                          >  2:881527/1‑150 (MQ=255)
                           gCCACAACGATCAGGCCAATCTGGTCTTTCTCCATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCag                                                                                                                        <  7:982808/151‑1 (MQ=255)
                           gCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCa                                                                                                                         <  5:564249/150‑1 (MQ=255)
                             cacaACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCag                                                                                                                        >  7:1004230/1‑149 (MQ=255)
                               caACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGgt                                                                                                                    <  1:649091/151‑1 (MQ=255)
                               caACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGgt                                                                                                                    <  6:1464788/151‑1 (MQ=255)
                               caACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGgt                                                                                                                    <  6:1354635/151‑1 (MQ=255)
                                aaCGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGgtg                                                                                                                   <  5:1293317/151‑1 (MQ=255)
                                  cGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTc                                                                                                                 <  5:1659074/151‑1 (MQ=255)
                                     tCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCTCGGAGACCGGTACGAGTGAGAATCCACTCGTCAGAGGTGTcccc                                                                                                              >  6:302892/1‑149 (MQ=255)
                                      cAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGGGTTCGCGGAGACCGGTACGAGTGACAATCCACGCGGCACAGGTGTccacc                                                                                                             >  4:1555640/1‑151 (MQ=255)
                                      cAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGGCGCCGCTTCAAAGCCCATGGTTGAAACGGGTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTccac                                                                                                              <  7:1176209/150‑1 (MQ=255)
                                       aGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTccacc                                                                                                             >  3:593718/1‑150 (MQ=255)
                                         gCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTTGCGCCGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCAtt                                                                                                          >  3:1428721/1‑151 (MQ=255)
                                          ccAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCAttt                                                                                                         <  8:950073/151‑1 (MQ=255)
                                          ccAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCAttt                                                                                                         >  3:1104335/1‑151 (MQ=255)
                                               cTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTcc                                                                                                    >  3:938599/1‑151 (MQ=255)
                                                 ggTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTcc                                                                                                    <  3:739599/149‑1 (MQ=255)
                                                 ggTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTc                                                                                                     <  7:1649239/148‑1 (MQ=255)
                                                 ggTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCa                                                                                                   >  8:827228/1‑150 (MQ=255)
                                                 ggTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCa                                                                                                   <  7:1142551/150‑1 (MQ=255)
                                                 ggTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTGTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCAtttttt                                                                                                      <  3:250762/147‑1 (MQ=255)
                                                  gTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCaaa                                                                                                 <  3:1573229/151‑1 (MQ=255)
                                                  gTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGGTGAAACGGTTTCGTTTGTCGCGGCAATGTGGCGTTCGCTGATACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCaaa                                                                                                 <  5:1359839/151‑1 (MQ=255)
                                                      ttCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACTATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCg                                                                                              <  6:726318/150‑1 (MQ=255)
                                                       tctcAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGATACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCgg                                                                                             <  6:528092/150‑1 (MQ=255)
                                                       tctcAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCgg                                                                                             <  1:1312739/150‑1 (MQ=255)
                                                        ctcAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCg                                                                                           >  5:374630/1‑151 (MQ=255)
                                                           aaTGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGttt                                                                                        <  1:402477/151‑1 (MQ=255)
                                                                 cGCCACCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGc                                                                                  >  8:1448376/1‑151 (MQ=255)
                                                                       ctcAATTGCGCGTGTCGCCGCTTCAAGGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGt                                                                            >  5:1680821/1‑151 (MQ=255)
                                                                        tcAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCCCTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGtt                                                                           >  4:766904/1‑151 (MQ=255)
                                                                         cAATTGCGCGGGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGtt                                                                           <  6:1067447/150‑1 (MQ=255)
                                                                           aTTGCGCGTGGCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCgg                                                                        <  6:1205910/151‑1 (MQ=255)
                                                                             tGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCggg                                                                       <  6:792717/150‑1 (MQ=255)
                                                                               ggcgTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGGAATGTGGCGTTCGCGGAGAGAGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACGATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAg                                                                    <  4:537876/150‑1 (MQ=255)
                                                                               cgcgTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAACTCGGCGTTTGTCCGCACTTGTTCGGGc                                                                      >  4:1348171/1‑149 (MQ=255)
                                                                                gcgTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGGTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGa                                                                   <  4:1133673/151‑1 (MQ=255)
                                                                                  gtgtCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATa                                                                 <  6:1250231/151‑1 (MQ=255)
                                                                                     gcgccgcTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCt                                                              <  5:67794/150‑1 (MQ=255)
                                                                                         cgcTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCa                                                          <  8:413673/151‑1 (MQ=255)
                                                                                             tCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTAc                                                      >  1:780840/1‑151 (MQ=255)
                                                                                              cAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTAcc                                                     <  1:1089815/151‑1 (MQ=255)
                                                                                              cAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTAc                                                      <  2:1056083/150‑1 (MQ=255)
                                                                                               aaaGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTAcc                                                     >  1:1151295/1‑150 (MQ=255)
                                                                                                 aGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGATACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCaat                                                  >  7:234365/1‑151 (MQ=255)
                                                                                                 aGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGAGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCaat                                                  <  5:1482919/151‑1 (MQ=255)
                                                                                                   cccATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCaataa                                                <  7:450163/151‑1 (MQ=255)
                                                                                                   cccATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCaata                                                 >  7:752361/1‑150 (MQ=255)
                                                                                                    ccATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGCCCGGTACGAGTGACACTCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCaataat                                               >  1:1006899/1‑151 (MQ=255)
                                                                                                     cATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATc                                              <  3:469092/151‑1 (MQ=255)
                                                                                                      aTGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCt                                             >  2:1657175/1‑151 (MQ=255)
                                                                                                      aTGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCt                                             <  7:880642/151‑1 (MQ=255)
                                                                                                        ggTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCtt                                            <  8:560027/150‑1 (MQ=255)
                                                                                                           tGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGt                                         >  3:1128515/1‑150 (MQ=255)
                                                                                                           tGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGt                                         >  7:1409804/1‑150 (MQ=255)
                                                                                                            taaaCGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGTGACCGGTACGAGTGACAATCCACTCGTCTTAGGTGTCCACCTTTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCATATAGCTGCCAGTAGCAATAATCTTCGta                                        <  5:1030336/149‑1 (MQ=255)
                                                                                                             aaaCGGTTTCGTTTGGCGCGTCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGtata                                      <  7:185369/151‑1 (MQ=255)
                                                                                                             aaaCGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGtata                                      <  5:96257/151‑1 (MQ=255)
                                                                                                             aaaCGGTTTCGTTTGGAGCGGCAATGTGGCGTTAGCGGACACCGGTACGAGTGAGAATCAACTCGTCAGAGGTGTCCACCATTTTTAGCAAATCGCCGTATGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGtat                                       >  2:1303294/1‑150 (MQ=255)
                                                                                                                 ggTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATg                                  >  1:1645962/1‑151 (MQ=255)
                                                                                                                    ttCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTa                                <  1:1534322/150‑1 (MQ=255)
                                                                                                                     tCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACg                              >  5:703537/1‑151 (MQ=255)
                                                                                                                     tCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACg                              >  6:1138789/1‑151 (MQ=255)
                                                                                                                      cGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGTCAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGc                             <  7:589831/151‑1 (MQ=255)
                                                                                                                       gTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCt                            >  1:572593/1‑151 (MQ=255)
                                                                                                                           ggCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAg                         <  7:1358644/150‑1 (MQ=255)
                                                                                                                           ggCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAg                         <  8:1117999/150‑1 (MQ=255)
                                                                                                                           ggCGCGGCAATGTGGCGGTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGt                        <  2:680105/151‑1 (MQ=255)
                                                                                                                              gcgGCAATGTGGCGTTCGAGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCAc                     >  4:1657281/1‑151 (MQ=255)
                                                                                                                               cgGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACt                    <  4:431218/151‑1 (MQ=255)
                                                                                                                                 gCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACttt                  <  3:397786/151‑1 (MQ=255)
                                                                                                                                 gCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACttt                  <  6:1556096/151‑1 (MQ=255)
                                                                                                                                  cAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACtttt                 >  7:1055926/1‑151 (MQ=255)
                                                                                                                                  cAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACtttt                 >  2:264124/1‑151 (MQ=255)
                                                                                                                                  cAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACtttt                 >  8:124290/1‑151 (MQ=255)
                                                                                                                                  cAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACtttt                 >  1:870699/1‑151 (MQ=255)
                                                                                                                                    aTGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCg               <  7:126748/151‑1 (MQ=255)
                                                                                                                                    aTGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCg               <  4:1476452/151‑1 (MQ=255)
                                                                                                                                          ggTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCATATGTGTCCACCATTTTTTCCAATTCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTtata         <  5:332398/150‑1 (MQ=255)
                                                                                                                                              cgcgGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTTATATAcc     <  1:1434080/151‑1 (MQ=255)
                                                                                                                                              cgcgGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTTATATAc      <  1:923900/150‑1 (MQ=255)
                                                                                                                                               gcgGAGACCGGTACGAGTGACAATCCACTCGTTAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTTATATACCg    <  8:275441/151‑1 (MQ=255)
                                                                                                                                               gcgGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTTATATACCg    <  4:8758/151‑1 (MQ=255)
                                                                                                                                               gcgGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTTATATACCg    <  2:1466501/151‑1 (MQ=255)
                                                                                                                                                cgGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTTATATACCGt   <  4:257917/151‑1 (MQ=255)
                                                                                                                                                cgGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTTATATACCGt   <  8:224529/151‑1 (MQ=255)
                                                                                                                                                 ggAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTTATATACCGTc  >  3:1611486/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGGAAAGCGTGCGTAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGATACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTTATATACCGTC  >  DH1/2730718‑2731013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑