Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 4,547,816 A→G Y80H (TAC→CAC)  rho ← transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,547,8160AG98.8% 1940.4 / 3.5 512Y80H (TAC→CAC) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base A (3/3);  new base G (251/252);  total (256/256)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.83e-01

Only 100 of 512 total aligned reads displayed.

TGCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTT  >  DH1/4547669‑4547963
                                                                                                                                                   |                                                                                                                                                   
tgCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGa                                                                                                                                                   <  5:1192359/150‑1 (MQ=255)
   gggCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGtc                                                                                                                                               <  3:279714/151‑1 (MQ=255)
    ggCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGtca                                                                                                                                              <  3:1512609/151‑1 (MQ=255)
     gCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGtcat                                                                                                                                             <  2:1872806/151‑1 (MQ=255)
       gTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGtcatc                                                                                                                                            >  4:1979654/1‑150 (MQ=255)
        ttttCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGtcatc                                                                                                                                            >  1:1280076/1‑149 (MQ=255)
           tCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGAc                                                                                                                                       >  4:507316/1‑151 (MQ=255)
           tCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGAc                                                                                                                                       >  3:175565/1‑151 (MQ=255)
            cAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGAcc                                                                                                                                      <  8:1376217/151‑1 (MQ=255)
             aGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCg                                                                                                                                     <  4:312345/151‑1 (MQ=255)
               gTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGGAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCAGCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGTGAAACGTGGATGTCATCAGGACCggg                                                                                                                                   >  8:1473135/1‑150 (MQ=255)
               gTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGc                                                                                                                                   >  8:783147/1‑151 (MQ=255)
                tttGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGGCCGGCg                                                                                                                                  >  8:361178/1‑151 (MQ=255)
                  tGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGa                                                                                                                                 <  1:1728061/150‑1 (MQ=255)
                     cGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGt                                                                                                                              <  5:1478266/150‑1 (MQ=255)
                     cGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTa                                                                                                                             <  4:869784/151‑1 (MQ=255)
                      gAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTGGGGGAAACGTGGATGTCATCAGGACCGGCGAGGt                                                                                                                              >  3:1019108/1‑149 (MQ=255)
                      gAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGt                                                                                                                              >  5:1367093/1‑149 (MQ=255)
                        aGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGa                                                                                                                          <  1:1607052/151‑1 (MQ=255)
                         gTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAg                                                                                                                         <  5:414417/151‑1 (MQ=255)
                             aCTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTgg                                                                                                                     >  5:1049506/1‑150 (MQ=255)
                                 cGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTg                                                                                                                  <  1:787685/150‑1 (MQ=255)
                                  gTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCg                                                                                                                <  5:887884/151‑1 (MQ=255)
                                  gTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCg                                                                                                                >  7:1249011/1‑151 (MQ=255)
                                    tAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCggc                                                                                                              >  7:382047/1‑150 (MQ=255)
                                    tAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCgg                                                                                                               <  1:348353/150‑1 (MQ=255)
                                       cTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAATGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACg                                                                                                           >  1:1947541/1‑151 (MQ=255)
                                        tttCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACgg                                                                                                          >  8:1595577/1‑151 (MQ=255)
                                        tttCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACgg                                                                                                          >  7:171994/1‑151 (MQ=255)
                                         ttCAGCAGCGCAAACTAGCGTTCACCTGCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGGGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACgg                                                                                                          <  6:251555/150‑1 (MQ=255)
                                         ttCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACgg                                                                                                          <  8:1531405/150‑1 (MQ=255)
                                          tCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACgg                                                                                                          >  2:1380833/1‑149 (MQ=255)
                                             gcagcGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGaa                                                                                                     <  5:758629/151‑1 (MQ=255)
                                                  gcAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCaa                                                                                                <  6:1740235/151‑1 (MQ=255)
                                                   cAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCaa                                                                                                >  2:449282/1‑150 (MQ=255)
                                                    aaaaTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCCGATTTGGATAGGGGAAACGTGGATGTCATCAGGACAGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAat                                                                                              >  3:134359/1‑151 (MQ=255)
                                                     aaaTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAGCCAAatc                                                                                             >  8:524529/1‑151 (MQ=255)
                                                        tAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTTTGACGAGTGCGGAGGTTGCAACGGGGGATTTGGCTAGGGGAAACGTGTATGTCATGAGGACCGGCGAGGTAGGAGCTGTCGGCGGAACGGAGGAAACCAAatcca                                                                                           >  6:765076/1‑150 (MQ=255)
                                                        tAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatccat                                                                                          >  4:404609/1‑151 (MQ=255)
                                                          gCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatccatcc                                                                                        <  1:100299/151‑1 (MQ=255)
                                                             ttCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGc                                                                                     >  5:1907766/1‑151 (MQ=255)
                                                              tCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGc                                                                                     >  2:1289660/1‑150 (MQ=255)
                                                               cACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCaa                                                                                   <  4:125423/151‑1 (MQ=255)
                                                                aCCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAat                                                                                  >  8:1691481/1‑151 (MQ=255)
                                                                  cttcttTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAatat                                                                                >  7:1561482/1‑151 (MQ=255)
                                                                    tcttTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAtc                                                                               <  2:311023/150‑1 (MQ=255)
                                                                      tttCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTcc                                                                            >  6:895839/1‑151 (MQ=255)
                                                                       ttCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAtctc                                                                             <  3:401493/149‑1 (MQ=255)
                                                                        tCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTcc                                                                            >  3:632003/1‑149 (MQ=255)
                                                                        tCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCa                                                                           <  1:1848848/150‑1 (MQ=255)
                                                                        tCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCa                                                                           <  2:1594922/150‑1 (MQ=255)
                                                                          ggcgggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTa                                                                        <  5:1672392/151‑1 (MQ=255)
                                                                              ggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGcc                                                                    <  3:1669318/151‑1 (MQ=255)
                                                                                 gAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATc                                                                 <  7:730711/151‑1 (MQ=255)
                                                                                 gAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATc                                                                 <  6:1179426/151‑1 (MQ=255)
                                                                                       tACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGTATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCaa                                                            >  3:1818647/1‑150 (MQ=255)
                                                                                       tACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCa                                                             <  4:158237/149‑1 (MQ=255)
                                                                                       tACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCAcc                                                              <  3:198363/148‑1 (MQ=255)
                                                                                        aCCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAg                                                          <  7:2004675/151‑1 (MQ=255)
                                                                                         ccAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCaa                                                            >  2:1640791/1‑148 (MQ=255)
                                                                                          cAGAGATTGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCaa                                                            >  1:616493/1‑147 (MQ=255)
                                                                                            gagaTGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGatat                                                      <  1:2029292/151‑1 (MQ=255)
                                                                                                tGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTc                                                  >  6:566657/1‑151 (MQ=255)
                                                                                                tGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTc                                                  >  8:1075491/1‑151 (MQ=255)
                                                                                                 ggTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTc                                                  <  7:966726/150‑1 (MQ=255)
                                                                                                  gTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGc                                                >  5:1254969/1‑151 (MQ=255)
                                                                                                  gTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGc                                                <  6:1941537/151‑1 (MQ=255)
                                                                                                    aTCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGcc                                               <  2:566443/150‑1 (MQ=255)
                                                                                                      cACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCAct                                            >  7:1061540/1‑151 (MQ=255)
                                                                                                       aCCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCActc                                           >  7:241893/1‑151 (MQ=255)
                                                                                                          aGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCttt                                        >  3:784455/1‑151 (MQ=255)
                                                                                                             gCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGc                                      <  1:1088092/150‑1 (MQ=255)
                                                                                                             gCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCg                                     >  1:507900/1‑151 (MQ=255)
                                                                                                              cGGAGGTTGAAACGGCGGATTTGGCTAGGGCAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCATTGCGt                                    >  8:932308/1‑151 (MQ=255)
                                                                                                                  ggTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGtgctg                                >  4:688992/1‑151 (MQ=255)
                                                                                                                   gTTGAAACGGCGGATTTGGCTAGGGTAAACGTTGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCTGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGtgctg                                <  4:134986/150‑1 (MQ=255)
                                                                                                                     tGAAACGGCGGTTTTGTATAGGGGAATCGTGGATTTAATCAGGACCTGAGAGGTAGGAGATGTATGCGGAACTGAGGAAAACAAATCCATACTGCAATATCTCCAGTACGAGATCACCAAAGATATCTTCGCCACACTTTGCGTGCTGCtt                             >  2:711984/1‑151 (MQ=255)
                                                                                                                     tGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTgctgct                              <  3:908480/150‑1 (MQ=255)
                                                                                                                        aaCGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGGTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAg                          <  7:450255/151‑1 (MQ=255)
                                                                                                                          cggcggATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTATTTGCGTGCTGCTTCAgg                         >  6:1868033/1‑150 (MQ=255)
                                                                                                                           ggcggATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGAt                       >  2:899888/1‑151 (MQ=255)
                                                                                                                           ggcggATTTCGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGAt                       <  6:1106375/151‑1 (MQ=255)
                                                                                                                            gcggATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATg                      <  6:1543472/151‑1 (MQ=255)
                                                                                                                             cggATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATgg                     >  5:964361/1‑151 (MQ=255)
                                                                                                                                   tGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCaaaa                >  8:722006/1‑150 (MQ=255)
                                                                                                                                    ggCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaat              <  6:360695/151‑1 (MQ=255)
                                                                                                                                     gCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaata             <  5:1114252/151‑1 (MQ=255)
                                                                                                                                     gCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTACTGCTTCAGGATGGCAAAaat              >  5:250475/1‑150 (MQ=255)
                                                                                                                                     gCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaat              >  8:426618/1‑150 (MQ=255)
                                                                                                                                      cTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaata             >  2:1117814/1‑150 (MQ=255)
                                                                                                                                       tAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaataat           >  2:1992505/1‑151 (MQ=255)
                                                                                                                                        aGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGTAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCAATCTTTGCGTGCTGCTTCAGGATGGCAAAaataat           <  4:1820922/150‑1 (MQ=255)
                                                                                                                                         ggggAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGt         <  6:1454356/151‑1 (MQ=255)
                                                                                                                                           ggAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTcc       >  8:1915662/1‑151 (MQ=255)
                                                                                                                                            gAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCt      >  1:1408444/1‑151 (MQ=255)
                                                                                                                                            gAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCt      <  3:555991/151‑1 (MQ=255)
                                                                                                                                            gAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCt      >  1:1179909/1‑151 (MQ=255)
                                                                                                                                             aaaCGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTg     <  8:213019/151‑1 (MQ=255)
                                                                                                                                               aCGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCt   >  8:1135809/1‑151 (MQ=255)
                                                                                                                                                cGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCtt  <  2:1026005/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTT  >  DH1/4547669‑4547963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: