Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 2,140,096 T→C E180G (GAA→GGA)  purR ← PurR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,140,0960TC99.6% 1858.7 / ‑3.2 480E180G (GAA→GGA) purRPurR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (253/223);  total (254/226)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 480 total aligned reads displayed.

GGCGCGATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTTCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCGCCA  >  DH1/2139948‑2140243
                                                                                                                                                    |                                                                                                                                                   
  cgcgATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCcgcg                                                                                                                                                 <  7:522169/151‑1 (MQ=255)
   gcgATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCgg                                                                                                                                                >  4:1428688/1‑151 (MQ=255)
    cgATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGt                                                                                                                                               <  4:1768141/151‑1 (MQ=255)
     gATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTg                                                                                                                                              >  8:261132/1‑151 (MQ=255)
       tAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGAc                                                                                                                                            >  1:691417/1‑151 (MQ=255)
        aaccccaTTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATAATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGCTGAcc                                                                                                                                           >  8:730652/7‑151 (MQ=255)
        aaCCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGAcc                                                                                                                                           >  3:704259/1‑151 (MQ=255)
         aCCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACcg                                                                                                                                          <  8:1311768/151‑1 (MQ=255)
          ccGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACcgc                                                                                                                                         <  1:442174/151‑1 (MQ=255)
            ggATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGt                                                                                                                                       <  4:161519/151‑1 (MQ=255)
            ggATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGt                                                                                                                                       >  1:1843203/1‑151 (MQ=255)
              aTTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTc                                                                                                                                     >  3:1197172/1‑151 (MQ=255)
                  aGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATc                                                                                                                                 <  6:1143843/151‑1 (MQ=255)
                      tCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATc                                                                                                                                 >  7:657223/1‑147 (MQ=255)
                       cAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATa                                                                                                                            <  2:1248131/151‑1 (MQ=255)
                       cAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATa                                                                                                                            <  5:1883177/151‑1 (MQ=255)
                        aaaGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATa                                                                                                                            >  8:546694/1‑150 (MQ=255)
                          aGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACg                                                                                                                         >  8:1436400/1‑151 (MQ=255)
                          aGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACg                                                                                                                         >  8:1092089/1‑151 (MQ=255)
                             cACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTGCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGccc                                                                                                                      <  1:172944/151‑1 (MQ=255)
                               cccTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCgg                                                                                                                    >  1:521101/1‑151 (MQ=255)
                                 cTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCATTCATAAAACCTGCAAGGCGGCCTGCTCCGGTGTTACGTTCAAGCGGGCCGGGGATGACGCCGATTCCGAGGTGAACGCGTTCATTCACATAACGCCAGGcc                                                                                                                  >  6:2033308/1‑151 (MQ=255)
                                  tGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGcc                                                                                                                  >  4:1341100/1‑150 (MQ=255)
                                  tGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGcc                                                                                                                  >  2:933328/1‑150 (MQ=255)
                                   gCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGcc                                                                                                                  <  3:1654448/149‑1 (MQ=255)
                                   gCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCAt                                                                                                                <  6:339700/151‑1 (MQ=255)
                                      caATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGt                                                                                                              >  3:281990/1‑150 (MQ=255)
                                       aaTCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGt                                                                                                              >  6:1905257/1‑149 (MQ=255)
                                          ccAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAgcc                                                                                                          <  6:1394608/150‑1 (MQ=255)
                                             gCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCt                                                                                                      <  6:1295606/151‑1 (MQ=255)
                                               tttCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCCGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTc                                                                                                    >  7:371103/1‑151 (MQ=255)
                                                ttCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTc                                                                                                    <  2:641042/150‑1 (MQ=255)
                                                ttCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTc                                                                                                    <  7:294463/150‑1 (MQ=255)
                                                 tCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCTGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCg                                                                                                   <  4:49700/150‑1 (MQ=255)
                                                   cGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAAc                                                                                                <  8:172465/151‑1 (MQ=255)
                                                    ggCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAAcg                                                                                               <  4:645424/151‑1 (MQ=255)
                                                     gCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAAcgc                                                                                              <  5:637888/151‑1 (MQ=255)
                                                     gCACCTTGATCATCGCTTCTTACATCGCCTTAATAAAACCGGCAAGGCGGCCTGCGCCGGTTTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCAGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGACTTCGAAcg                                                                                               >  8:1181476/1‑150 (MQ=255)
                                                       aCCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGt                                                                                            <  7:858042/151‑1 (MQ=255)
                                                        ccTTTATCATCGCTTCATCCATCGCCTTCATAAAACCGGAAAGGCGGCCTGAGCCTGTGTTACGTTCCAGCGGGCCGTGTATGACGCCGATTCCGCGTTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGtt                                                                                           <  2:313884/151‑1 (MQ=255)
                                                        ccTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGtt                                                                                           >  5:422660/1‑151 (MQ=255)
                                                         cTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGAGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGtt                                                                                           >  7:1787519/1‑150 (MQ=255)
                                                           tGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTAt                                                                                         <  5:200419/150‑1 (MQ=255)
                                                           tGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATc                                                                                        >  7:21208/1‑151 (MQ=255)
                                                            gATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATc                                                                                        >  7:1310279/1‑150 (MQ=255)
                                                            gATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATc                                                                                        <  1:1539697/150‑1 (MQ=255)
                                                             atcatcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCTGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCaa                                                                                      <  3:1398724/151‑1 (MQ=255)
                                                             atcatcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCa                                                                                       >  3:402219/1‑150 (MQ=255)
                                                              tcatcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAAt                                                                                     >  3:483786/1‑151 (MQ=255)
                                                              tcatcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAAt                                                                                     >  2:1455677/1‑151 (MQ=255)
                                                               catcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAAt                                                                                     <  3:617609/150‑1 (MQ=255)
                                                               catcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATg                                                                                    <  2:2041648/151‑1 (MQ=255)
                                                               catcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATg                                                                                    >  5:859778/1‑151 (MQ=255)
                                                                atcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATg                                                                                    >  5:1417960/1‑150 (MQ=255)
                                                                  cGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGAcc                                                                                 >  4:786388/1‑151 (MQ=255)
                                                                   gCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCg                                                                                >  8:1877969/1‑151 (MQ=255)
                                                                    cttcttCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACATTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGc                                                                               >  6:475327/1‑151 (MQ=255)
                                                                      tcttcCCTCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCa                                                                              <  1:1019887/150‑1 (MQ=255)
                                                                         tcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCAt                                                                             <  5:270664/148‑1 (MQ=255)
                                                                         tcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATc                                                                            <  4:1019302/149‑1 (MQ=255)
                                                                         tcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCg                                                                           <  1:100235/150‑1 (MQ=255)
                                                                          ccATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCgg                                                                          <  1:2012079/150‑1 (MQ=255)
                                                                          ccATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGt                                                                         >  7:1560891/1‑151 (MQ=255)
                                                                             tCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGa                                                                       >  6:671498/1‑150 (MQ=255)
                                                                              cGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGa                                                                       >  8:874637/1‑149 (MQ=255)
                                                                                ccTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTc                                                                   <  1:887841/151‑1 (MQ=255)
                                                                                     aTAAAACCGTCAAGGCGGCCTTCGGCGGTTTTACGTTCCAGCGGGCCGTGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCt                                                               <  8:1970608/150‑1 (MQ=255)
                                                                                     aTAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCtt                                                              <  4:1954974/151‑1 (MQ=255)
                                                                                       aaaaCCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCtttt                                                            <  4:224642/151‑1 (MQ=255)
                                                                                       aaaaCCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCttt                                                             <  1:1305923/150‑1 (MQ=255)
                                                                                             ggCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTCCTTc                                                       >  3:857521/1‑150 (MQ=255)
                                                                                                aaGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTCGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCAccc                                                   >  6:397788/1‑151 (MQ=255)
                                                                                                 aGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCGCGGCCATGTAGCAGCCTTCGAACGCGTTATCATAGACCGCATCGGTCAAGTCAGCTTTTGCTTCAcccc                                                  >  2:109402/1‑151 (MQ=255)
                                                                                                     ggcCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTc                                              >  5:204221/1‑151 (MQ=255)
                                                                                                       ccTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCa                                            <  1:1438083/151‑1 (MQ=255)
                                                                                                       ccTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCa                                            <  6:900715/151‑1 (MQ=255)
                                                                                                        cTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCAt                                           >  3:1592369/1‑151 (MQ=255)
                                                                                                         tGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATg                                          >  3:1900266/1‑151 (MQ=255)
                                                                                                           cgcCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGac                                        >  2:907375/1‑151 (MQ=255)
                                                                                                             ccGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGacc                                       >  6:150616/1‑150 (MQ=255)
                                                                                                               ggTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGaccacc                                    >  5:174346/1‑151 (MQ=255)
                                                                                                                gtgtTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGaccacc                                    >  5:966402/1‑150 (MQ=255)
                                                                                                                  gtTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCAtt                                 >  8:1467668/1‑151 (MQ=255)
                                                                                                                    tACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTg                                <  2:476642/150‑1 (MQ=255)
                                                                                                                     aCGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTggg                              >  5:1540917/1‑151 (MQ=255)
                                                                                                                      cGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTggg                              >  3:547487/1‑150 (MQ=255)
                                                                                                                       gTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGat                            >  4:1161151/1‑151 (MQ=255)
                                                                                                                          ggaGCGGGGCGGGGCTGACGCCGAATCCGGGGGGACCGCGTTCAATGACTTAACGGCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCGAGTCCATGACCACGATTGGGatat                          <  2:1633398/148‑1 (MQ=255)
                                                                                                                          ccAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGatat                          <  7:1092171/150‑1 (MQ=255)
                                                                                                                            aGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGc                       <  7:1236548/151‑1 (MQ=255)
                                                                                                                             gCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCg                      <  8:1277457/151‑1 (MQ=255)
                                                                                                                              cGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGCCCACCATTGGGATATGGCg                      >  5:752277/1‑150 (MQ=255)
                                                                                                                                   cGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATActc                >  8:361248/1‑151 (MQ=255)
                                                                                                                                    ggggATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATActct               >  1:266127/1‑151 (MQ=255)
                                                                                                                                          gACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCACCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGc         <  3:173894/151‑1 (MQ=255)
                                                                                                                                            cGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCAt       <  6:16127/151‑1 (MQ=255)
                                                                                                                                              ccGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCg     >  2:1875335/1‑151 (MQ=255)
                                                                                                                                               cGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCGc    >  5:1345467/1‑151 (MQ=255)
                                                                                                                                               cGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCGc    >  3:101083/1‑151 (MQ=255)
                                                                                                                                                 aTTCCGCGGTGACCGCGGTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCGCCa  <  5:1003023/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCGCGATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTTCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCGCCA  >  DH1/2139948‑2140243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑