Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 1,768,973 C→T T168I (ACA→ATA)  wzxB → polisoprenol‑linked O‑antigen transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,768,9730CT98.7% 1664.4 / 4.3 449T168I (ACA→ATA) wzxBpolisoprenol‑linked O‑antigen transporter
Reads supporting (aligned to +/- strand):  ref base C (2/4);  new base T (223/220);  total (225/224)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 454 total aligned reads displayed.

TTATATGGTCGTTTGTTCCTGCATTAGTCGGGAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACACAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCGATCTTTAGCAGACGGTTT  >  DH1/1768826‑1769123
                                                                                                                                                   |                                                                                                                                                      
 tataTGGTCGTTTGTTCCTGCATTAGTCGGGAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATTAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTAATATACCTCTAACATTTATTTTTGTGAACATaaat                                                                                                                                                    >  3:1682332/1‑150 (MQ=255)
  atatGGTCGTTTGTTCCTGCATTAGTCGGGAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGt                                                                                                                                                   <  1:1420615/151‑1 (MQ=255)
   tatGGTCGTTTGTTCCTGCATTAGTCGGGAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGt                                                                                                                                                   >  7:1645416/1‑150 (MQ=255)
         cGTTTGTTCCTGCATTAGTCGGGAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTAAGatat                                                                                                                                            >  8:1345033/1‑151 (MQ=255)
                 ccTGCATTAGTCGGGAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAAtt                                                                                                                                     <  2:814767/150‑1 (MQ=255)
                 ccTGCATTAGTCGGGAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTg                                                                                                                                    >  5:160334/1‑151 (MQ=255)
                 ccTGCATTAGTCGGGAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTg                                                                                                                                    >  6:1072540/1‑151 (MQ=255)
                      aTTAGTCGGGAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGt                                                                                                                               >  1:179207/1‑151 (MQ=255)
                      aTTAGTCGGGAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGt                                                                                                                               >  2:325846/1‑151 (MQ=255)
                        tAGTCGGGAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGtt                                                                                                                              <  6:5372/150‑1 (MQ=255)
                          gTCGGGAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTa                                                                                                                           <  3:234249/151‑1 (MQ=255)
                          gTCGGGAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAAAATAAAGTCAGATATAGCAATTGCCGGtttt                                                                                                                            >  6:43811/1‑150 (MQ=255)
                            cGGGAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTAtt                                                                                                                         >  2:98096/1‑151 (MQ=255)
                            cGGGAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTAt                                                                                                                          <  4:580394/150‑1 (MQ=255)
                             gggAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTc                                                                                                                        >  1:63840/1‑151 (MQ=255)
                             gggAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTc                                                                                                                        <  7:1781570/151‑1 (MQ=255)
                              ggAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTc                                                                                                                        >  7:1497959/1‑150 (MQ=255)
                                   ttAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCa                                                                                                                   <  8:125073/150‑1 (MQ=255)
                                        cTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTTAAGTga                                                                                                              >  5:1434152/1‑149 (MQ=255)
                                          aCCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGc                                                                                                              <  5:561317/148‑1 (MQ=255)
                                          aCCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGc                                                                                                              <  8:622569/148‑1 (MQ=255)
                                           cccTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAAt                                                                                                          <  6:1607353/151‑1 (MQ=255)
                                             cTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCt                                                                                                        >  3:1212689/1‑151 (MQ=255)
                                               aTCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATcn                                                                                                        <  2:1498721/149‑2 (MQ=255)
                                                 cTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGtt                                                                                                    >  5:902161/1‑151 (MQ=255)
                                                 cTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGtt                                                                                                    >  5:650464/1‑151 (MQ=255)
                                                   ggCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGtt                                                                                                    <  4:1147837/149‑1 (MQ=255)
                                                   ggCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGtt                                                                                                    <  4:156267/149‑1 (MQ=255)
                                                   ggCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGATTTTATTCAGTCAAGTGCAAATCTGGTTGc                                                                                                  >  5:185146/1‑151 (MQ=255)
                                                      tGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTgg                                                                                               >  1:1215397/1‑151 (MQ=255)
                                                      tGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTgg                                                                                               >  3:458018/1‑151 (MQ=255)
                                                      tGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTgg                                                                                               >  2:114976/1‑151 (MQ=255)
                                                       gTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTgg                                                                                               >  6:118826/1‑150 (MQ=255)
                                                        tttCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGa                                                                                              >  7:782897/1‑150 (MQ=255)
                                                                 aaagaaaaAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCAc                                                                                    >  3:57724/1‑151 (MQ=255)
                                                                   agaaaaAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACt                                                                                   >  2:630944/1‑150 (MQ=255)
                                                                    gaaaaAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACt                                                                                   >  3:410050/1‑149 (MQ=255)
                                                                      aaaaaTGAATTGGCTGACTTTAAGTAGTATTTTATCGCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCt                                                                               <  8:823245/151‑1 (MQ=255)
                                                                      aaaaaTGAAATGTCTGACTTTAAGTAGTATTTTATGCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGGTTTATTCAGTCAAGTGCAAATCTGGGTGCTGGAATTATTGCACTAggt                                                                               <  6:1726287/151‑3 (MQ=255)
                                                                      aaaaaTGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCt                                                                               <  8:1185028/151‑1 (MQ=255)
                                                                       aaaaTGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCt                                                                               >  2:144791/1‑150 (MQ=255)
                                                                        aaatgaaatgGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTAt                                                                             >  1:429259/1‑151 (MQ=255)
                                                                         aatgaaatgGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATc                                                                            <  2:1685387/151‑1 (MQ=255)
                                                                         aatgaaatgGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGAAATTGCCGCTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATc                                                                            >  1:1600006/1‑151 (MQ=255)
                                                                            gaaatgGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCgt                                                                          <  4:237090/150‑1 (MQ=255)
                                                                              aatgGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTAATCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCgttgt                                                                       >  7:1352019/1‑151 (MQ=255)
                                                                               atgGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCgttgtt                                                                      <  7:466515/151‑1 (MQ=255)
                                                                               atgGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCgttgtt                                                                      <  3:1116804/151‑1 (MQ=255)
                                                                                tgGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTc                                                                     >  4:1115453/1‑151 (MQ=255)
                                                                                    tGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATg                                                                  >  7:1608052/1‑150 (MQ=255)
                                                                                    tGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATg                                                                  >  4:454442/1‑150 (MQ=255)
                                                                                     gACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATg                                                                  <  5:827070/149‑1 (MQ=255)
                                                                                     gACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGa                                                                 >  8:1813807/1‑150 (MQ=255)
                                                                                      aCTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGa                                                                 >  4:1436674/1‑149 (MQ=255)
                                                                                       cTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCATCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAgg                                                              >  8:312687/1‑151 (MQ=255)
                                                                                          tAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTg                                                           >  7:1689615/1‑151 (MQ=255)
                                                                                           aaGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTgg                                                          <  8:480712/151‑1 (MQ=255)
                                                                                            agtagtATTTTATCCCGCTTGGCTATTATCCCTCTAAGATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGa                                                         <  8:1653874/151‑1 (MQ=255)
                                                                                               agtaTTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTg                                                      >  2:559516/1‑151 (MQ=255)
                                                                                                      tATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGt                                               >  4:633211/1‑151 (MQ=255)
                                                                                                       aTCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGt                                               <  3:840886/150‑1 (MQ=255)
                                                                                                        tCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGtt                                              >  3:1582409/1‑150 (MQ=255)
                                                                                                         cccGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTAc                                            <  7:863881/151‑1 (MQ=255)
                                                                                                         cccGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTAc                                            >  6:250676/1‑151 (MQ=255)
                                                                                                          ccGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACg                                           >  8:1090120/1‑151 (MQ=255)
                                                                                                          ccGCTTGGCTATTATACCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTAAAGTGCAAATCTCGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTCGTAAAGTTACg                                           >  4:864858/1‑151 (MQ=255)
                                                                                                          ccGCTTGGCAATTATCCCTCTAACATTTATTTTAGTGAACATAAAGACAGATATAGCAATTGCAGGTTTAATTAAGTCAAGTGCAAATCTTGTTGATGGAATTAATGCACTAGCTATCGTTGTTCATGAAGTTTGTATTGGTAAACTTAcc                                           >  1:762758/1‑150 (MQ=255)
                                                                                                           cGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGc                                          >  6:685341/1‑151 (MQ=255)
                                                                                                            gCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTTGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCt                                         <  7:573340/151‑1 (MQ=255)
                                                                                                            gCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCt                                         >  4:727158/1‑151 (MQ=255)
                                                                                                               tGGCTATTATCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATc                                      >  5:1520446/1‑151 (MQ=255)
                                                                                                                   tattatCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCAtt                                   >  3:1446544/1‑150 (MQ=255)
                                                                                                                   tattatCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCAtt                                   >  6:1544179/1‑150 (MQ=255)
                                                                                                                    attatCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGAAAATCTGGTTGCTGGAATTATTGCACTAGATATCGTTGTTCATGAAGGTTGTATTGGTAAAGTTACTCTATCATTa                                  >  2:370387/1‑150 (MQ=255)
                                                                                                                      tatCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACa                                <  1:248232/150‑1 (MQ=255)
                                                                                                                       atCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACAt                               >  1:374107/1‑150 (MQ=255)
                                                                                                                       atCCCTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACAt                               <  5:1065842/150‑1 (MQ=255)
                                                                                                                         cccTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAAt                            <  4:792149/151‑1 (MQ=255)
                                                                                                                          ccTCTAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAAtg                           <  5:129104/151‑1 (MQ=255)
                                                                                                                           ctctAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAAtgt                          >  3:982073/1‑151 (MQ=255)
                                                                                                                              tAACATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGc                        <  4:1685387/150‑1 (MQ=255)
                                                                                                                               aaCATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGc                        <  2:104011/149‑1 (MQ=255)
                                                                                                                               aaCATTTATTTTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCg                       <  5:171917/150‑1 (MQ=255)
                                                                                                                                  atttatttTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCg                    >  3:1289955/1‑150 (MQ=255)
                                                                                                                                  atttatttTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCg                    >  8:1732799/1‑150 (MQ=255)
                                                                                                                                   tttatttTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCGAt                  >  4:1228468/1‑151 (MQ=255)
                                                                                                                                    atatttTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCGAt                  <  8:386778/149‑1 (MQ=255)
                                                                                                                                     tatttTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCGATCt                >  5:479252/1‑151 (MQ=255)
                                                                                                                                      atttTTGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCGATCt                <  1:1046899/150‑1 (MQ=255)
                                                                                                                                        ttttGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCGATCtttt             >  7:1060029/1‑150 (MQ=255)
                                                                                                                                          ttGTGAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCGATCTTTAGc           >  7:119167/1‑151 (MQ=255)
                                                                                                                                           tgtgAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCGATCTTTAGc           >  8:146876/1‑150 (MQ=255)
                                                                                                                                            gtgAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCGATCTTTAGCa          <  3:726471/150‑1 (MQ=255)
                                                                                                                                              gAACATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCGATCTTTAGCAGa        <  3:634290/150‑1 (MQ=255)
                                                                                                                                               aaCATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATTTGCGTCGATCTTTAGCAGACg      >  6:983669/1‑151 (MQ=255)
                                                                                                                                               aaCATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCGATCTTTAGCAGACg      >  5:1332138/1‑151 (MQ=255)
                                                                                                                                               aaCATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCGATCTTTAGCAGACg      <  7:325765/151‑1 (MQ=255)
                                                                                                                                               aaCATAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCGATCTTTAGCAGACg      <  2:708256/151‑1 (MQ=255)
                                                                                                                                                acatAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAACTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCGATCTTTAGCAGACgg     >  6:860784/1‑151 (MQ=255)
                                                                                                                                                   caAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCGATCTTTAGCAGACGGttt  >  5:236570/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
TTATATGGTCGTTTGTTCCTGCATTAGTCGGGAATTTAATCTACCCTATCTGGCTGTTTCAGGGAAAAGAAAAAATGAAATGGCTGACTTTAAGTAGTATTTTATCCCGCTTGGCTATTATCCCTCTAACATTTATTTTTGTGAACACAAAGTCAGATATAGCAATTGCCGGTTTTATTCAGTCAAGTGCAAATCTGGTTGCTGGAATTATTGCACTAGCTATCGTTGTTCATGAAGGTTGGATTGGTAAAGTTACGCTATCATTACATAATGTGCGTCGATCTTTAGCAGACGGTTT  >  DH1/1768826‑1769123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: