BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	DH1	1,299,401	G→T	A389S (GCG→TCG)	aegA →	protein AegA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	1,299,401	0	G	T	99.1%	1644.3 / 2.8	440	A389S (GCG→TCG)	aegA	protein AegA
Reads supporting (aligned to +/- strand): ref base G (1/3); new base T (198/238); total (199/241)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01

Only 100 of 443 total aligned reads displayed.

GCTGGCCTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCGCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATGCGCCGGGCG  >  DH1/1299253‑1299548

 cTGGCCTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGctcg                                                                                                                                                   >  3:1383997/1‑150 (MQ=255)

  tGGCCTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGAt                                                                                                                                                 >  5:1406366/1‑151 (MQ=255)

   ggCCTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATg                                                                                                                                                <  2:1753267/151‑1 (MQ=255)

   ggCCTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATg                                                                                                                                                <  3:1550197/151‑1 (MQ=255)

    gCCTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATgg                                                                                                                                               >  4:696693/1‑151 (MQ=255)

    gCCTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGCTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCCCTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCCCGCCGGCGGGAAATCTTCAGCTCGATgg                                                                                                                                               >  2:390744/1‑151 (MQ=255)

      cTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATgggg                                                                                                                                             >  2:859484/1‑151 (MQ=255)

       tgtgCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATgggg                                                                                                                                             >  6:1502128/1‑150 (MQ=255)

           cGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTc                                                                                                                                         >  1:30337/1‑150 (MQ=255)

             gATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACt                                                                                                                                      <  7:1780513/151‑1 (MQ=255)

                gTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTc                                                                                                                                    <  4:1034856/150‑1 (MQ=255)

                gTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCg                                                                                                                                   <  8:1437342/151‑1 (MQ=255)

                  tCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGa                                                                                                                                  >  7:1158009/1‑150 (MQ=255)

                     gACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTc                                                                                                                              >  8:1171882/1‑151 (MQ=255)

                         cggaaTGGCGTGGGGGTGACGGGGTACGCTCGCCATGCAGAAATCGGTGGCGTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCAGGCCGTCGGGAAATCTTCAGCTCGATGGGGTTTGAGTTGGAACTCAAtt                                                                                                                          <  2:526789/148‑1 (MQ=255)

                          gCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATtg                                                                                                                         <  2:270153/151‑1 (MQ=255)

                            aaTGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATtgtg                                                                                                                       >  8:1313444/1‑151 (MQ=255)

                             aTGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGa                                                                                                                      <  7:204175/151‑1 (MQ=255)

                               ggCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAg                                                                                                                    <  1:918209/151‑1 (MQ=255)

                               ggCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCGCGATGGGGATTCACTTCGAACTCAATTGTGAAg                                                                                                                    <  7:1663770/151‑1 (MQ=255)

                                 cGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTg                                                                                                                  >  2:1397898/1‑151 (MQ=255)

                                     ggggTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGt                                                                                                               >  4:427801/1‑150 (MQ=255)

                                     ggggTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTa                                                                                                              <  3:1209068/151‑1 (MQ=255)

                                      gggTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTaa                                                                                                             <  3:1313295/151‑1 (MQ=255)

                                      gggTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTaa                                                                                                             <  3:1583001/151‑1 (MQ=255)

                                      gggTGACGGGGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTaa                                                                                                             <  5:734149/151‑1 (MQ=255)

                                           aCGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTTCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATg                                                                                                        <  5:101848/151‑1 (MQ=255)

                                             ggTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGtc                                                                                                      >  8:1834925/1‑151 (MQ=255)

                                                 tACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCtt                                                                                                  >  3:55822/1‑151 (MQ=255)

                                                    gATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTgg                                                                                               >  1:1261916/1‑151 (MQ=255)

                                                       cGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTGTCTTTGGAtt                                                                                            >  8:745253/1‑151 (MQ=255)

                                                       cGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGAtt                                                                                            <  6:460959/151‑1 (MQ=255)

                                                       cGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGAt                                                                                             <  2:1709498/150‑1 (MQ=255)

                                                        gCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTc                                                                                           <  6:1267304/151‑1 (MQ=255)

                                                        gCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTc                                                                                           <  7:496498/151‑1 (MQ=255)

                                                         ccATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCg                                                                                          >  6:1772224/1‑151 (MQ=255)

                                                          cATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGc                                                                                         >  3:649687/1‑151 (MQ=255)

                                                          cATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGc                                                                                         <  2:1184093/151‑1 (MQ=255)

                                                             ccAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCttt                                                                                      <  8:1695237/151‑1 (MQ=255)

                                                              cAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCtttt                                                                                     >  7:971645/1‑151 (MQ=255)

                                                                gAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTgg                                                                                   >  4:550928/1‑151 (MQ=255)

                                                                   aTCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAAc                                                                                >  5:1231267/1‑151 (MQ=255)

                                                                   aTCGGTGGCTTGCTCACTCTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAAc                                                                                <  4:17521/151‑1 (MQ=255)

                                                                     cGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCGCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACa                                                                               >  7:31510/1‑150 (MQ=255)

                                                                      ggTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAAt                                                                             >  6:1575987/1‑151 (MQ=255)

                                                                      ggTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAAt                                                                             <  4:874159/151‑1 (MQ=255)

                                                                      ggTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAAt                                                                             <  3:387771/151‑1 (MQ=255)

                                                                       gTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAAt                                                                             >  6:619534/1‑150 (MQ=255)

                                                                       gTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATa                                                                            <  2:872496/151‑1 (MQ=255)

                                                                        tGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATac                                                                           >  5:311351/1‑151 (MQ=255)

                                                                         ggCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATac                                                                           <  6:1122297/150‑1 (MQ=255)

                                                                         ggCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATac                                                                           <  7:1595328/150‑1 (MQ=255)

                                                                         ggCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCGCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATac                                                                           <  7:1446879/150‑1 (MQ=255)

                                                                          gCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATacga                                                                         <  1:1830907/151‑1 (MQ=255)

                                                                            ttGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTATAGATGTCTCTTTGCATTCGCTTTTGGAACAATacgacg                                                                       >  6:846377/1‑151 (MQ=255)

                                                                             tGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATacgac                                                                        <  2:1579446/149‑1 (MQ=255)

                                                                                 cACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTc                                                                  >  1:631862/1‑151 (MQ=255)

                                                                                  aCTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCt                                                                 >  7:615604/1‑151 (MQ=255)

                                                                                     ttCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCg                                                              >  5:1297067/1‑151 (MQ=255)

                                                                                       cGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGtt                                                            <  1:1522644/151‑1 (MQ=255)

                                                                                       cGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGtt                                                            >  8:60278/1‑151 (MQ=255)

                                                                                        ggCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTg                                                           <  3:260560/151‑1 (MQ=255)

                                                                                          gaTTCCTTCTTTCAAACTTGCTAAATCCCGTCGGGCCCGCCGGCGGGCAATGGTCAGGTGGATGGGGATTCCATTGGAACTCAATTGTGCAGTGGGGAAAGCTGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGc                                                         <  3:181419/150‑1 (MQ=255)

                                                                                           aTTCCTTCTTTCAACCTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGACGTGGGTAAAGCTGGCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCg                                                        <  3:1792402/151‑1 (MQ=255)

                                                                                             tCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGt                                                       <  4:1820739/150‑1 (MQ=255)

                                                                                              ccTTCTTTCAAACTTGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGGGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGCGGCGGTCTTCGTTGGCGt                                                       >  4:746462/1‑149 (MQ=255)

                                                                                              ccTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGt                                                       >  7:1264664/1‑149 (MQ=255)

                                                                                               cttcttTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAgg                                                    >  8:1563993/1‑151 (MQ=255)

                                                                                               cttcttTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAgg                                                    >  4:1431238/1‑151 (MQ=255)

                                                                                               cttcttTCAAACTGGATAAATCCCTGCTGGCACGACGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGCGTAAAGATGTCTCTTAGGATTCGCTTTTGGAACAATACGACGCTGTCTTCGTTGGCGTAgc                                                    >  4:198606/1‑150 (MQ=255)

                                                                                                 tcttTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGcc                                                  >  1:580718/1‑150 (MQ=255)

                                                                                                   tttCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACt                                                >  5:97082/1‑151 (MQ=255)

                                                                                                      cAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTAc                                             <  5:871376/151‑1 (MQ=255)

                                                                                                       aaaCTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTAcc                                            >  1:1014975/1‑151 (MQ=255)

                                                                                                         aCTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGt                                          >  2:1538829/1‑151 (MQ=255)

                                                                                                            ggATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTcc                                       >  1:1108884/1‑151 (MQ=255)

                                                                                                              aTAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCAt                                     >  2:565476/1‑151 (MQ=255)

                                                                                                                aaaTCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATg                                    <  7:1061594/150‑1 (MQ=255)

                                                                                                                aaaTCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCATTTGGAACAATACGACGCGGTCTTCGTTGGCGAAGGCACTTAACGTTCCATg                                    >  2:699529/1‑150 (MQ=255)

                                                                                                                     ccTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCCCTGCGCACTCAATGGTGAAGTGGGTAAAGCTGTCTCTTTGGATTCGCTTTTGGAACAATACGCCGCGGGCGTCGTTGGCGTAGGGACTTGCCGGTCCCTGATAGCg                              >  7:1558337/1‑151 (MQ=255)

                                                                                                                      ctgctgGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCgg                             >  6:3637/1‑151 (MQ=255)

                                                                                                                      ctgctgGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCgg                             >  8:267566/1‑151 (MQ=255)

                                                                                                                       tgctgGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGGGTAGGGACTTACCGTTCCATGAACGCggg                            >  5:1653710/1‑151 (MQ=255)

                                                                                                                        gctgGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCgg                             <  5:40153/149‑1 (MQ=255)

                                                                                                                        gctgGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGt                           >  8:1030556/1‑151 (MQ=255)

                                                                                                                          tgGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGttt                         >  1:788885/1‑151 (MQ=255)

                                                                                                                              aCGCCGTCGTGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTAccc                     <  7:163991/151‑1 (MQ=255)

                                                                                                                                gCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCa                    >  7:746694/1‑150 (MQ=255)

                                                                                                                                gCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGGCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCaa                   <  2:1311629/151‑1 (MQ=255)

                                                                                                                                  cGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATg                 <  4:298655/151‑1 (MQ=255)

                                                                                                                                   gTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGa                <  1:1633518/151‑1 (MQ=255)

                                                                                                                                     ttggAAATGTTCAGCTCGTTGGGGATTCACTTCGTACTCATTTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGATCATTACGACGCGGTCTTGGTTGGCGTTGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGaa               <  6:1041638/148‑1 (MQ=255)

                                                                                                                                     cGGGAAATCTTCAGCTCGATGGGGATGCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCGTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGaa               <  2:1019517/150‑1 (MQ=255)

                                                                                                                                      gggAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGa                >  3:937986/1‑148 (MQ=255)

                                                                                                                                      gggAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGCCTTTCACCAATGAAgc             >  3:304303/1‑150 (MQ=255)

                                                                                                                                        gAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATg           <  7:855390/151‑1 (MQ=255)

                                                                                                                                        gAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGATGGAGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATg           >  6:974503/1‑151 (MQ=255)

                                                                                                                                             cTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATGCGCCg      >  5:1572039/1‑151 (MQ=255)

                                                                                                                                               tCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATGCGCCg      <  4:1280816/149‑1 (MQ=255)

                                                                                                                                                cAGCTCGATGGTGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATGCGCCggg    <  5:619712/150‑1 (MQ=255)

GCTGGCCTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCGCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATGCGCCGGGCG  >  DH1/1299253‑1299548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`