Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 4,547,561 C→G A165P (GCA→CCA)  rho ← transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,547,5610CG98.9% 2158.5 / 1.4 563A165P (GCA→CCA) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base G (242/314);  total (246/317)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 563 total aligned reads displayed.

CCGGACGTTCGTCGATCAGCAGAACCATCAGCACACAATCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGCCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAAC  >  DH1/4547414‑4547708
                                                                                                                                                   |                                                                                                                                                   
    aCGTTCGTCGATCAGCAGAACCATCAGCACACAATCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATcc                                                                                                                                              <  5:1739902/151‑1 (MQ=255)
     cGTTCGTCGATCAGCAGAACCATCAGCACACAATCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCa                                                                                                                                             <  3:1409154/151‑1 (MQ=255)
         cgtcgATCAGCAGAACCATCAGCACACAATCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTAc                                                                                                                                         <  7:218311/151‑1 (MQ=255)
         cgtcgATCAGCAGAACCATCAGCACACAATCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTCTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTa                                                                                                                                          <  3:850452/150‑1 (MQ=255)
          gtcgATCAGCAGAACCATCAGCACACAATCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTAcg                                                                                                                                        <  1:943152/151‑1 (MQ=255)
               tCAGCAGAACCATCAGCACACAATCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGATCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCCCGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGc                                                                                                                                   >  2:789561/1‑151 (MQ=255)
                cagcagAACCATCAGCACACAATCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCa                                                                                                                                  <  8:806875/151‑1 (MQ=255)
                 agcagAACCATCAGCACACAATCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAg                                                                                                                                 <  3:199388/151‑1 (MQ=255)
                     gAACCATCAGCACACAATCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTa                                                                                                                              >  1:1018917/1‑150 (MQ=255)
                            cAGCACACAATCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTc                                                                                                                       <  5:867242/150‑1 (MQ=255)
                              gCACACAATCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGt                                                                                                                    <  3:1184362/151‑1 (MQ=255)
                                 cacaATCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGt                                                                                                                    >  7:1147316/1‑148 (MQ=255)
                                    aaTCCGGGTGTTTGTAAGCAATGCTCTGTGCAATGTTCTGCAGCAGGTTGGTTTTACCGGCTTTCGGCGGTGGCACAATCAGACCACGCTGACGACGACCGATTGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATGTTCAGTAGAAcc                                                                                                              <  6:1376110/151‑1 (MQ=255)
                                    aaTCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAAcc                                                                                                              >  5:622141/1‑151 (MQ=255)
                                     aTCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCg                                                                                                             <  5:939798/151‑1 (MQ=255)
                                     aTCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCg                                                                                                             <  1:1190506/151‑1 (MQ=255)
                                       ccGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTGTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCACACCAGGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGtt                                                                                                           <  7:866605/151‑1 (MQ=255)
                                        ggggTGGTTGTAAGCAATGCTCTGAGCATTGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTa                                                                                                          <  7:1017358/150‑1 (MQ=255)
                                        cGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTa                                                                                                          <  7:1769305/151‑1 (MQ=255)
                                          ggtggtTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTAcc                                                                                                        >  8:1218960/1‑151 (MQ=255)
                                           gtggtTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTAcc                                                                                                        >  3:100216/1‑150 (MQ=255)
                                           gtggtTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTAcc                                                                                                        >  3:1293794/1‑150 (MQ=255)
                                            gggtTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCAc                                                                                                      <  2:672884/150‑1 (MQ=255)
                                                tGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTc                                                                                                  >  3:1138726/1‑151 (MQ=255)
                                                 gTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGTCCACGCTGACCACGTCCGATAGGTGATGGCAGATCCAGTACTCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTc                                                                                                  <  8:1308068/150‑1 (MQ=255)
                                                 gTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTcc                                                                                                 >  8:40818/1‑151 (MQ=255)
                                                 gTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTcc                                                                                                 <  8:516239/151‑1 (MQ=255)
                                                 gTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTc                                                                                                  <  2:870069/150‑1 (MQ=255)
                                                   aaGCAATGCTCTGAGCTATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCa                                                                                                <  5:463280/150‑1 (MQ=255)
                                                   aaGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCAt                                                                                               >  1:303114/1‑151 (MQ=255)
                                                   aaGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCAt                                                                                               <  4:1109978/151‑1 (MQ=255)
                                                   aaGCAATGCTCTGAGCAAGGTGCTGCAGCAGCATGGGTTTACCGGCTTGCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCAt                                                                                               <  6:341245/151‑1 (MQ=255)
                                                    aGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATa                                                                                              <  5:1341664/151‑1 (MQ=255)
                                                     gCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATAc                                                                                             >  3:596850/1‑151 (MQ=255)
                                                         tGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGc                                                                                           >  1:1079112/1‑149 (MQ=255)
                                                           ctctGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGAGATGGCAGATCCAGAACGAGAGCAGTTAAATCTTCAGAAGAACCGTTACAACCTTCCATACGAAGAc                                                                                       >  8:1842402/1‑151 (MQ=255)
                                                                  cAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGtt                                                                                >  7:1390098/1‑151 (MQ=255)
                                                                   aaTGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGttt                                                                               >  1:1478995/1‑151 (MQ=255)
                                                                   aaTGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGttt                                                                               >  4:1361239/1‑151 (MQ=255)
                                                                   aaTGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGCCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGACttt                                                                               >  6:84766/1‑151 (MQ=255)
                                                                          ggcagcagcaTGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCa                                                                        <  6:555525/150‑1 (MQ=255)
                                                                           gcagcagcaTGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGc                                                                         <  2:1160778/149‑1 (MQ=255)
                                                                           gcagcagcaTGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCa                                                                        <  1:1353559/150‑1 (MQ=255)
                                                                           gcagcagcaTGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAg                                                                       <  4:1437173/151‑1 (MQ=255)
                                                                            cagcagcaTGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGc                                                                      >  4:1037888/1‑151 (MQ=255)
                                                                             agcagcaTGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCg                                                                     >  2:1450937/1‑151 (MQ=255)
                                                                             agcagcaTGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCg                                                                     >  7:491943/1‑151 (MQ=255)
                                                                             agcagcaTGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCg                                                                     >  8:293649/1‑151 (MQ=255)
                                                                              gcagcaTGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTCCCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCgg                                                                    <  5:1608341/151‑1 (MQ=255)
                                                                                agcaTGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCgcgg                                                                  >  2:366769/1‑151 (MQ=255)
                                                                                 gcaTGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGt                                                                 <  7:735457/151‑1 (MQ=255)
                                                                                  caTGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGtt                                                                <  1:466288/151‑1 (MQ=255)
                                                                                  caTGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGtt                                                                <  2:1326496/151‑1 (MQ=255)
                                                                                   aTGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTa                                                               <  1:807416/151‑1 (MQ=255)
                                                                                     ggTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTa                                                               >  1:1812379/1‑149 (MQ=255)
                                                                                        tttACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGttg                                                          >  1:747241/1‑150 (MQ=255)
                                                                                           aCCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTtctc                                                        >  5:237973/1‑150 (MQ=255)
                                                                                           aCCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTtctc                                                        >  5:342409/1‑150 (MQ=255)
                                                                                             cGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCaaa                                                     <  3:648713/151‑1 (MQ=255)
                                                                                               gCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCaa                                                      >  6:1705719/1‑148 (MQ=255)
                                                                                                cTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAag                                                    >  8:1625606/1‑149 (MQ=255)
                                                                                                  ttCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAagag                                                  <  4:690860/149‑1 (MQ=255)
                                                                                                  ttCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAgg                                                 >  5:1091887/1‑150 (MQ=255)
                                                                                                     ggcggTGCCCCAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGCACCGTTACCACGGTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTGTCAAAGAGGAttt                                             >  5:732291/1‑151 (MQ=255)
                                                                                                     ggcggTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGAttt                                             >  8:683098/1‑151 (MQ=255)
                                                                                                     ggcggTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGCGGTTAAGTTCTCAAAGAGGAttt                                             <  5:1268636/151‑1 (MQ=255)
                                                                                                         gTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTttgtt                                         <  6:1517926/151‑1 (MQ=255)
                                                                                                          tGCCACAATCAGACCGCGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTttgttg                                        >  4:175838/1‑151 (MQ=255)
                                                                                                              acaATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCggg                                    <  3:750384/151‑1 (MQ=255)
                                                                                                              acaATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCggg                                    <  6:767505/151‑1 (MQ=255)
                                                                                                              acaATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCggg                                    <  4:26381/151‑1 (MQ=255)
                                                                                                               caATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGc                                   <  4:1618991/151‑1 (MQ=255)
                                                                                                               caATCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGc                                   <  4:830028/151‑1 (MQ=255)
                                                                                                                  tCAGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGtt                                >  6:1035713/1‑151 (MQ=255)
                                                                                                                  tCAGACCACGCTGAACACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGtt                                >  7:327071/1‑151 (MQ=255)
                                                                                                                    aGACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGtttt                              <  6:1834487/151‑1 (MQ=255)
                                                                                                                     gACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTc                             >  3:388872/1‑151 (MQ=255)
                                                                                                                     gACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTc                             <  7:155548/151‑1 (MQ=255)
                                                                                                                     gACCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCACCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGtttt                              >  8:101517/1‑150 (MQ=255)
                                                                                                                     caCCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTc                             <  5:145542/150‑1 (MQ=255)
                                                                                                                      aCCACGCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCTTACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCa                            <  2:1677259/151‑1 (MQ=255)
                                                                                                                         aCGGTGACCTCGACGGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTTGAAGCGTTTCCACGTGCCATACGCAGTCGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGt                         <  2:553932/151‑1 (MQ=255)
                                                                                                                           gCTGACCACGACCTATAGGTGATGGCAGATCCAGTAAGCGAGCTGTTAAATCTTCAGTAGAACCGTTACCAGGTTCCATACGCAGACGAGATTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGAGTTTTCAGGttt                       <  6:986768/151‑1 (MQ=255)
                                                                                                                           gCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGttt                       <  1:1655905/151‑1 (MQ=255)
                                                                                                                           gCTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGttt                       <  2:10031/151‑1 (MQ=255)
                                                                                                                            cTGACCACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGTTTg                      >  7:1455928/1‑151 (MQ=255)
                                                                                                                                 cACGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGTTTGTCGaa                 <  2:1765693/151‑1 (MQ=255)
                                                                                                                                  aCGACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACTTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAATGAGGATTTTCTTGCGGGCGTTTTCAGGTTTGTCGa                  >  2:1558139/1‑149 (MQ=255)
                                                                                                                                   cGACCGATAGGTGATGGCGGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGTTTGTCGAAGt               >  8:1717375/1‑151 (MQ=255)
                                                                                                                                    gACCGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGTTTGTCGAAGtt              <  8:1409198/151‑1 (MQ=255)
                                                                                                                                      ccGATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGTTTGTCGAAGTTaa            <  4:521624/151‑1 (MQ=255)
                                                                                                                                        gATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCCGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGTTTGTCGAAGTTAACt          >  6:460884/1‑151 (MQ=255)
                                                                                                                                        gATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAGGAGGATTTTGTTGCGGGCGTTTTCAGGTTTGTCGAAGTTAACt          <  3:1605108/151‑1 (MQ=255)
                                                                                                                                        gATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGTTTGTCGAAGTTAACt          <  6:125538/151‑1 (MQ=255)
                                                                                                                                        gATAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGTTTGTCGAAGTTAACt          <  7:1265113/151‑1 (MQ=255)
                                                                                                                                         aTAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGTTTGTCGAAGTTAACtt         <  3:502461/151‑1 (MQ=255)
                                                                                                                                          tAGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTc        >  4:312040/1‑151 (MQ=255)
                                                                                                                                           aGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTc        <  4:1280812/150‑1 (MQ=255)
                                                                                                                                           aGGTGATGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCg       >  1:564530/1‑151 (MQ=255)
                                                                                                                                                aTGGCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAAc  >  7:389607/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCGGACGTTCGTCGATCAGCAGAACCATCAGCACACAATCCGGGTGGTTGTAAGCAATGCTCTGAGCAATGTTCTGCAGCAGCATGGTTTTACCGGCTTTCGGCGGTGCCACAATCAGACCACGCTGACCACGACCGATAGGTGATGCCAGATCCAGTACGCGAGCAGTTAAATCTTCAGTAGAACCGTTACCACGTTCCATACGCAGACGAGAGTTTGCGTGCAGCGGGGTTAAGTTCTCAAAGAGGATTTTGTTGCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAAC  >  DH1/4547414‑4547708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑