Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 4,137,730 C→A intergenic (‑24/+152) sugE ← / ← ecnB quaternary ammonium compound‑resistance protein SugE/entericidin B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,137,7300CA97.9% 2260.3 / 26.5 607intergenic (‑24/+152)sugE/ecnBquaternary ammonium compound‑resistance protein SugE/entericidin B
Reads supporting (aligned to +/- strand):  ref base C (6/7);  new base A (309/284);  total (316/291)
Fisher's exact test for biased strand distribution p-value = 7.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 607 total aligned reads displayed.

CTGACAATCATCGCCGTCACAGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTACCGTTA  >  DH1/4137582‑4137877
                                                                                                                                                    |                                                                                                                                                   
 tGACAATCATCGCCGTCACAGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTc                                                                                                                                                  >  5:790354/1‑151 (MQ=255)
  gACAATCATCGCCGTCACAGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTcc                                                                                                                                                 >  5:1748014/1‑151 (MQ=255)
        catcGCCGTCACAGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTg                                                                                                                                           <  8:1014306/151‑1 (MQ=255)
        catcGCCGTCACAGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCACGGTg                                                                                                                                           >  8:881675/1‑151 (MQ=255)
        catcCCCGTCACAGTAATAAGACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGt                                                                                                                                            <  4:728623/150‑1 (MQ=255)
               gTCACAGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAag                                                                                                                                      >  5:866758/1‑149 (MQ=255)
                tCACAGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAgg                                                                                                                                   >  4:648873/1‑151 (MQ=255)
                tCACAGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAgg                                                                                                                                   >  1:715888/1‑151 (MQ=255)
                 cacaGTAATAACACTCGGCGTCAAACGAGTATAGCCGTGGTTATATTTCAGGCCAACGGCCCATACGACTTCCAGCAGACCAGCAATAACTAATATAATCCAGGACATATCAGGCTGCGGAGAATTGGGGCAGTCCCCGTTGAAATGAgg                                                                                                                                   <  1:491543/150‑1 (MQ=255)
                  acaGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCg                                                                                                                                 >  8:274611/1‑151 (MQ=255)
                  acaGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCg                                                                                                                                 >  1:1732874/1‑151 (MQ=255)
                  acaGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATACAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCg                                                                                                                                 >  3:597749/1‑151 (MQ=255)
                   caGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGt                                                                                                                                <  7:336374/151‑1 (MQ=255)
                       aataaCACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTg                                                                                                                             <  5:148452/150‑1 (MQ=255)
                       aataaCACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGc                                                                                                                            >  6:455110/1‑151 (MQ=255)
                        ataaCACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCa                                                                                                                           <  2:1553214/151‑1 (MQ=255)
                        ataaCACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCa                                                                                                                           <  7:61162/151‑1 (MQ=255)
                          aaCACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAgg                                                                                                                         >  3:172300/1‑151 (MQ=255)
                                gggCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTcc                                                                                                                   <  6:1036453/150‑1 (MQ=255)
                                cGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGgtcgtc                                                                                                                    <  7:809870/150‑1 (MQ=255)
                                cGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTcc                                                                                                                   >  4:374604/1‑151 (MQ=255)
                                    gTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCa                                                                                                               <  6:223089/151‑1 (MQ=255)
                                      cAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCaaa                                                                                                             <  5:1123042/151‑1 (MQ=255)
                                      cAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCaa                                                                                                              >  7:1138191/1‑150 (MQ=255)
                                         aCGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCt                                                                                                          <  1:635777/151‑1 (MQ=255)
                                           gACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTg                                                                                                         >  7:1020214/1‑150 (MQ=255)
                                           gACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGa                                                                                                        <  1:1243935/151‑1 (MQ=255)
                                                  gCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGa                                                                                                 <  8:1435263/151‑1 (MQ=255)
                                                   ccGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGaa                                                                                                <  8:52615/151‑1 (MQ=255)
                                                   ccGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGa                                                                                                 >  5:1276799/1‑150 (MQ=255)
                                                    cGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAAc                                                                                               >  6:1350036/1‑151 (MQ=255)
                                                    cGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAAc                                                                                               >  2:172726/1‑151 (MQ=255)
                                                     gTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACg                                                                                              >  2:550876/1‑151 (MQ=255)
                                                     gTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACg                                                                                              >  3:1223078/1‑151 (MQ=255)
                                                       gggTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGa                                                                                            <  2:703337/151‑1 (MQ=255)
                                                        ggTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGat                                                                                           <  1:111867/151‑1 (MQ=255)
                                                        ggTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGat                                                                                           <  6:615521/151‑1 (MQ=255)
                                                        ggTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCACGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATTGTTGAACGGat                                                                                           <  2:1233299/151‑1 (MQ=255)
                                                         gTATATTTCAGGCCAACGGCCCATACCGCTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGata                                                                                          <  3:179162/151‑1 (MQ=255)
                                                           atatTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATAtt                                                                                        >  4:1659837/1‑151 (MQ=255)
                                                              tttCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTg                                                                                     >  8:638818/1‑151 (MQ=255)
                                                                 cAGGTCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGcc                                                                                   >  8:759599/1‑150 (MQ=255)
                                                                 cAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCa                                                                                  <  4:763230/151‑1 (MQ=255)
                                                                 cAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCa                                                                                  <  4:303640/151‑1 (MQ=255)
                                                                 cAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGCAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGcc                                                                                   >  3:476493/1‑150 (MQ=255)
                                                                  aGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAg                                                                                 <  6:34689/151‑1 (MQ=255)
                                                                   ggCCAACGGCCCATACCACTTCCAGCAGACCATCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAgg                                                                                <  7:1549299/151‑1 (MQ=255)
                                                                        aCGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGc                                                                            >  6:869417/1‑150 (MQ=255)
                                                                         cGGCCCATTCCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGc                                                                            >  2:885160/1‑149 (MQ=255)
                                                                         cGGCCCATACCACTTCCAGCAGACGAGCAATAACTAAGATAATCCAGGACATATGAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAg                                                                          <  1:1565388/151‑1 (MQ=255)
                                                                          ggCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAgg                                                                         >  4:1776872/1‑151 (MQ=255)
                                                                          ggCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAgg                                                                         >  3:1088006/1‑151 (MQ=255)
                                                                          ggCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCATGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAgg                                                                         >  7:1327286/1‑151 (MQ=255)
                                                                              cATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGa                                                                      >  3:864793/1‑150 (MQ=255)
                                                                                tACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAAtt                                                                   >  3:716550/1‑151 (MQ=255)
                                                                                aaCCACTTCCAGCAGACCAGCAATAACTAAGATACTCCAGGACATATCGGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAAtt                                                                   <  7:780088/150‑1 (MQ=255)
                                                                                  ccACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTc                                                                 >  2:1633129/1‑151 (MQ=255)
                                                                                   cACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGAACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTc                                                                 >  1:1466528/1‑150 (MQ=255)
                                                                                      ttCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACt                                                             >  3:803828/1‑151 (MQ=255)
                                                                                       tCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACt                                                             <  7:468815/150‑1 (MQ=255)
                                                                                        gcaggagACGAGCTATAACTAAGATAATCCAGGAGATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGGTGATGGTTGAAGGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTAt                                                           <  8:390500/150‑1 (MQ=255)
                                                                                          agcagACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTa                                                         <  8:1775273/151‑1 (MQ=255)
                                                                                                ccAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTtg                                                   >  4:1016982/1‑151 (MQ=255)
                                                                                                ccAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTtg                                                   <  3:1319617/151‑1 (MQ=255)
                                                                                                  aGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTtgtg                                                 >  1:1036411/1‑151 (MQ=255)
                                                                                                  aGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTtgtg                                                 >  3:209072/1‑151 (MQ=255)
                                                                                                    cAATATCTAAGATAATCCAGGACATATCTGGCTCCGGAGAATTGGGTGAGTCCGCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGGCAGGTGGGAGGGGAATTTCAACTATTACCTTTGTGAc                                               <  8:1675741/151‑1 (MQ=255)
                                                                                                     aaTAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGGAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACt                                              <  1:276297/151‑1 (MQ=255)
                                                                                                     aaTAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACt                                              >  1:1313164/1‑151 (MQ=255)
                                                                                                     aaTAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACt                                              <  2:136776/151‑1 (MQ=255)
                                                                                                      aTAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACt                                              >  4:1337782/1‑150 (MQ=255)
                                                                                                       tAACTAAGATAATCCAGGACATATCAGACTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGCTGGTTGAACGGATATTTTGCCAGGGGGCCGGGGAAGTTCAACTATTACCTTTGTGACTAt                                            >  8:136046/1‑151 (MQ=255)
                                                                                                        aaCTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTAt                                            <  8:1511972/150‑1 (MQ=255)
                                                                                                            aaGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGc                                         <  2:610764/149‑1 (MQ=255)
                                                                                                            aaGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCa                                        <  8:1225557/150‑1 (MQ=255)
                                                                                                             aGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGc                                         >  6:1156502/1‑148 (MQ=255)
                                                                                                               aTAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGaa                                     >  2:1130218/1‑150 (MQ=255)
                                                                                                                tAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGAGATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGaaa                                    >  5:1834435/1‑150 (MQ=255)
                                                                                                                 aaTCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGaaaaa                                  <  1:83581/151‑1 (MQ=255)
                                                                                                                  aTCCAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTTGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGaaaaaa                                 <  5:486816/151‑1 (MQ=255)
                                                                                                                      aGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAaaacaaa                             <  7:1635006/151‑1 (MQ=255)
                                                                                                                      aGGACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAaaacaa                              >  4:1458403/1‑150 (MQ=255)
                                                                                                                       ggACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCTGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAaaagaaac                            <  7:1279314/151‑1 (MQ=255)
                                                                                                                        gACATATCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACg                           <  7:1839157/151‑1 (MQ=255)
                                                                                                                         aCATATCAGGCTCCGGAGAATTGGGGCAGTCCCAGGTGAAAAGAGGCGTTTGAAGGTCGTGCTGCAAACATTATGGTTGAAAGGATATTTTGCCATGAGGCAGGGGAATTTCAACTATTAGCATTGTGACTATCGCAGAAAAAACAAACgg                          >  2:421920/1‑151 (MQ=255)
                                                                                                                             atCATGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCAc                       <  5:1708635/150‑1 (MQ=255)
                                                                                                                             atCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCAc                       <  1:408265/150‑1 (MQ=255)
                                                                                                                             atCAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTAACTTTGTGACTATCGCAGAAAAAACAAACGGCACg                      >  5:349254/1‑151 (MQ=255)
                                                                                                                               cAGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGac                    >  4:550653/1‑151 (MQ=255)
                                                                                                                                aGGCTCCGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGacc                   >  6:1643832/1‑150 (MQ=255)
                                                                                                                                     cctgagaATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCt              <  7:1416690/148‑1 (MQ=255)
                                                                                                                                     ccGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCt              <  8:1086030/151‑1 (MQ=255)
                                                                                                                                     ccGGAGAATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCt              >  6:1571396/1‑151 (MQ=255)
                                                                                                                                          gaATTGGGGCGGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGACCGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAACCGGCACGACACAGCTGTCGt         >  1:966847/1‑151 (MQ=255)
                                                                                                                                          gaATTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGt         >  2:276059/1‑151 (MQ=255)
                                                                                                                                            aTTGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTAc       <  4:195137/151‑1 (MQ=255)
                                                                                                                                             ttGGGGCAGTCGCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGTGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTAc       <  4:1176707/150‑1 (MQ=255)
                                                                                                                                             ttGGGGCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTAcc      >  7:280980/1‑151 (MQ=255)
                                                                                                                                                 ggCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTACCGtta  <  3:1538358/151‑1 (MQ=255)
                                                                                                                                                 ggCAGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTACCGtta  <  5:650138/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTGACAATCATCGCCGTCACAGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTACCGTTA  >  DH1/4137582‑4137877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: