Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 3,795,806 A→G intergenic (+73/‑91) sgrT → / → tbpA transcriptional regulator SgrR/thiamine‑binding periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,795,8060AG97.6% 1858.4 / 16.3 499intergenic (+73/‑91)sgrT/tbpAtranscriptional regulator SgrR/thiamine‑binding periplasmic protein
Reads supporting (aligned to +/- strand):  ref base A (7/5);  new base G (222/263);  total (230/269)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.11e-01

Only 100 of 516 total aligned reads displayed.

ATGCGCGGCCTGCGCATGAATACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGAAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCCTGCTGTTGCTGTGCACAGCGCCCGTTTTCGCTAAACCC  >  DH1/3795659‑3795956
                                                                                                                                                   |                                                                                                                                                      
aTGCGCGGCCTGCGCATGAATACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGgaa                                                                                                                                                      <  6:687260/150‑1 (MQ=255)
  gcgcGGCCTGCGCATGAATACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAAt                                                                                                                                                    <  5:1120874/150‑1 (MQ=255)
  gcgcGGCCTGCGCATGAATACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAAt                                                                                                                                                    <  6:1336803/150‑1 (MQ=255)
     cgGCCTGCGCATGAATACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATAccc                                                                                                                                                <  1:963754/151‑1 (MQ=255)
     cgGCCTGCGCATGAATACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATAccc                                                                                                                                                >  1:450197/1‑151 (MQ=255)
      ggCCTGCGCATGAATACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCg                                                                                                                                               <  8:868283/151‑1 (MQ=255)
      ggCCTGCGCATGAATACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCg                                                                                                                                               <  5:1588487/151‑1 (MQ=255)
      ggCCTGCGCATGAATACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCg                                                                                                                                               >  8:1463193/1‑151 (MQ=255)
       gCCTGCGCATGAATACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGt                                                                                                                                              >  4:402514/1‑151 (MQ=255)
        ccTGCGCATGAATACACTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTTCGCTGAGGAAATACCCGTc                                                                                                                                             >  6:1642310/1‑151 (MQ=255)
         cTGCGCATGAATACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCg                                                                                                                                            >  5:1755451/1‑151 (MQ=255)
          ggcgcATGAATACCCTCGGCGGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGa                                                                                                                                           <  7:590675/150‑1 (MQ=255)
           gcgcATGAATACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCg                                                                                                                                            <  2:1214376/149‑1 (MQ=255)
           gcgcATGAATACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGa                                                                                                                                           <  7:1543525/150‑1 (MQ=255)
             gcATGAATACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAAcc                                                                                                                                        >  4:331385/1‑151 (MQ=255)
                 gAATACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGAt                                                                                                                                    <  1:5686/151‑1 (MQ=255)
                   aTACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATcc                                                                                                                                  >  1:745345/1‑151 (MQ=255)
                   aTACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATcc                                                                                                                                  >  8:474265/1‑151 (MQ=255)
                   aTACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATAACCGTCGAACCTGATcc                                                                                                                                  <  7:480235/151‑1 (MQ=255)
                          cGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATa                                                                                                                             <  5:1490738/149‑1 (MQ=255)
                           ggCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATACCg                                                                                                                          >  3:1090517/1‑151 (MQ=255)
                           ggCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACg                                                                                                                          >  5:133562/1‑151 (MQ=255)
                           ggCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACg                                                                                                                          <  8:739551/151‑1 (MQ=255)
                           ggCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACg                                                                                                                          >  1:1417725/1‑151 (MQ=255)
                           ggCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACg                                                                                                                          <  6:7985/151‑1 (MQ=255)
                            gCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGc                                                                                                                         <  8:1641540/151‑1 (MQ=255)
                              tGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCg                                                                                                                       >  4:1061404/1‑151 (MQ=255)
                               ggTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGcc                                                                                                                        <  5:520975/149‑1 (MQ=255)
                               ggTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCgg                                                                                                                      >  5:986882/1‑151 (MQ=255)
                                gTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTGAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGc                                                                                                                     <  1:294420/151‑1 (MQ=255)
                                   cGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGa                                                                                                                   >  4:1270807/1‑150 (MQ=255)
                                       tttAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAggg                                                                                                               <  1:1500914/150‑1 (MQ=255)
                                        ttAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGa                                                                                                              <  5:838694/150‑1 (MQ=255)
                                        ttAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGAt                                                                                                             >  5:674599/1‑151 (MQ=255)
                                         tAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGAtt                                                                                                            >  4:1337218/1‑151 (MQ=255)
                                           aaTCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTg                                                                                                          >  2:486472/1‑151 (MQ=255)
                                              caggctGGATTGCCCCACCGGATCCCTGATTGCTGGCTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAg                                                                                                        <  7:213974/145‑1 (MQ=255)
                                              cAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAgg                                                                                                       >  1:336976/1‑151 (MQ=255)
                                                gCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCAGGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCt                                                                                                     <  5:1184530/151‑1 (MQ=255)
                                                gCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCt                                                                                                     <  7:1394167/151‑1 (MQ=255)
                                                  gTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTcc                                                                                                   >  6:932392/1‑151 (MQ=255)
                                                     gTTTGCGCCACCGGATCAATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCAAGCGTACGCGGGCGCTGAGTAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTtc                                                                                                >  2:723425/1‑151 (MQ=255)
                                                       ttGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTtct                                                                                               <  2:263336/150‑1 (MQ=255)
                                                        tGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTtctc                                                                                              <  3:1785135/150‑1 (MQ=255)
                                                         gcgcCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCaa                                                                                            <  5:769497/151‑1 (MQ=255)
                                                          cgcCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCa                                                                                             >  4:1571582/1‑149 (MQ=255)
                                                           gcCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGt                                                                                          >  7:1658614/1‑151 (MQ=255)
                                                           gcCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGt                                                                                          >  2:1420460/1‑151 (MQ=255)
                                                               ccGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGAAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCtt                                                                                      >  3:1331803/1‑151 (MQ=255)
                                                                cGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCttt                                                                                     >  6:1061338/1‑151 (MQ=255)
                                                                 ggATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTg                                                                                    <  3:821546/151‑1 (MQ=255)
                                                                 ggATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTg                                                                                    <  1:1247837/151‑1 (MQ=255)
                                                                  gATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGc                                                                                   <  4:26494/151‑1 (MQ=255)
                                                                  gATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGc                                                                                   >  8:1234157/1‑151 (MQ=255)
                                                                   aTCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGcc                                                                                  >  7:1310530/1‑151 (MQ=255)
                                                                    tCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGGGGCTCCTTCTCAAGTCCTTTGcc                                                                                  >  1:1088540/1‑150 (MQ=255)
                                                                     ccATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCAc                                                                                >  1:1647445/1‑151 (MQ=255)
                                                                      cATGATTGCTGGTTGATAACAAAAGCACTACACTAACGCCGTGCTCACCGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCAct                                                                               <  6:1769293/151‑1 (MQ=255)
                                                                       aggATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCtctc                                                                              >  5:271082/3‑151 (MQ=255)
                                                                         gATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCActct                                                                             <  2:958137/150‑1 (MQ=255)
                                                                         gATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCtt                                                                            >  8:1070200/1‑151 (MQ=255)
                                                                           ttGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCtttt                                                                          >  3:635149/1‑151 (MQ=255)
                                                                            tGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCt                                                                                                     >  7:1211996/1‑123 (MQ=255)
                                                                            tGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCtttt                                                                          <  6:450635/150‑1 (MQ=255)
                                                                             gCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCtttttt                                                                        <  2:606943/151‑1 (MQ=255)
                                                                              cTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTg                                                                       >  4:925273/1‑151 (MQ=255)
                                                                              cTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCAGTCTCAAGTCCTTTGCAGCTCttttttt                                                                       >  2:1693885/1‑150 (MQ=255)
                                                                                 gTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAgg                                                                    >  6:344861/1‑151 (MQ=255)
                                                                                   tGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGt                                                                   <  3:743540/150‑1 (MQ=255)
                                                                                    gATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTCTTTTGAGGTGc                                                                 >  4:807552/1‑151 (MQ=255)
                                                                                       aaCAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCaaa                                                              <  3:298837/151‑1 (MQ=255)
                                                                                           aaaTCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAgtgt                                                          <  7:1464216/151‑1 (MQ=255)
                                                                                            aaTCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGtt                                                         >  1:1804809/1‑151 (MQ=255)
                                                                                             aTCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGtt                                                         >  8:132488/1‑150 (MQ=255)
                                                                                               cactacactaACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTaa                                                       <  3:477130/150‑1 (MQ=255)
                                                                                                   acactaACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTaaaaaa                                                   <  2:1104443/150‑1 (MQ=255)
                                                                                                   acactaACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGAAAATACCCGTCAAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTaaaaaaa                                                  <  3:1205452/151‑1 (MQ=255)
                                                                                                    cactaACGCCGTTCTCAACGGGGTGTCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAAt                                                 >  1:1068256/1‑151 (MQ=255)
                                                                                                     actaACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATg                                                <  3:886297/151‑1 (MQ=255)
                                                                                                        aaCGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGt                                               <  6:1233345/149‑1 (MQ=255)
                                                                                                        aaCGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCt                                             <  7:1741617/151‑1 (MQ=255)
                                                                                                        aaCGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCt                                             >  7:838696/1‑151 (MQ=255)
                                                                                                             cGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGcccc                                        >  4:591454/1‑151 (MQ=255)
                                                                                                              gTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCct                                       <  3:529395/151‑1 (MQ=255)
                                                                                                              gTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCct                                       >  8:801435/1‑151 (MQ=255)
                                                                                                              gTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCct                                       >  7:956381/1‑151 (MQ=255)
                                                                                                                  tcAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCctgctg                                   >  7:68136/1‑151 (MQ=255)
                                                                                                                   cAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCctgctg                                   <  3:1637462/150‑1 (MQ=255)
                                                                                                                   cAACGGGGTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCctgc                                     <  4:700986/148‑1 (MQ=255)
                                                                                                                        gggTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCCtgctgttgctg                             >  2:179574/1‑151 (MQ=255)
                                                                                                                        gggTGCCACGCGTACGCGTGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCCtgctgttgctg                             <  1:684794/151‑1 (MQ=255)
                                                                                                                                cgcgtacgcgtGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCCTGCTGTTGCTGTGCACAgc                     <  2:1537718/151‑1 (MQ=255)
                                                                                                                                     acgcgtGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCCTGCTGTTGCTGTGCACAGCGccc                 <  4:1467449/150‑1 (MQ=255)
                                                                                                                                     acgcgtGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCCTGCTGTTGCTGTGCACAGCGCCCg                <  6:1405703/151‑1 (MQ=255)
                                                                                                                                       gcgtGCGCTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCCTGCTGTTGCTGTGCACAGCGCCCGtt              >  8:1365452/1‑151 (MQ=255)
                                                                                                                                            cgcTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCCTGCTGTTGCTGTGCACAGCGCCCGTTTTCGc         <  6:1494806/151‑1 (MQ=255)
                                                                                                                                              cTGAGGAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCCTGCTGTTGCTGTGCACAGCGCCCGTTTTCGCt        >  3:1387015/1‑150 (MQ=255)
                                                                                                                                                gaggAACTACCCGTCGAACCTGATCCGGATACCGCCGGCGAAGGGAGTTGAGGCTCCTTCTCAAGTCCTTTCCCACTCTTTATTGAGGTGCAAAGTGGTAAAAAAATGTCTGCCCCTGCTGTTGCTGTGCACAGCGCCCGTTTTCGCTa       <  8:1810182/149‑1 (MQ=255)
                                                                                                                                                gaggAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCCTGCTGTTGCTGTGCACAGCGCCCGTTTTCGCTaa      <  4:1704336/150‑1 (MQ=255)
                                                                                                                                                   aaaaTACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCCTGCTGTTGATGTGCACAGCGCCCGTTTACGCTAAAacc  >  8:355807/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
ATGCGCGGCCTGCGCATGAATACCCTCGGCTGGTTCGATTTTAAATCAGCGTGGTTTGCGCCACCGGATCCATGATTGCTGGTTGATAACAAAATCACTACACTAACGCCGTTCTCAACGGGGTGCCACGCGTACGCGTGCGCTGAGAAAATACCCGTCGAACCTGATCCGGATAACGCCGGCGAAGGGATTTGAGGCTCCTTCTCAAGTCCTTTGCCACTCTTTTTTGAGGTGCAAAGTGTTAAAAAAATGTCTGCCCCTGCTGTTGCTGTGCACAGCGCCCGTTTTCGCTAAACCC  >  DH1/3795659‑3795956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: