Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,184,5850CA25.4% 366.5 / 35.5 182intergenic (+8/‑30)ECDH1_RS15795/ECDH1_RS15800tRNA‑Gln/tRNA‑TERM
Reads supporting (aligned to +/- strand):  ref base C (84/51);  new base A (41/5);  total (126/56)
Fisher's exact test for biased strand distribution p-value = 4.29e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 187 total aligned reads displayed.

CCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTC  >  DH1/3184440‑3184734
                                                                                                                                                 |                                                                                                                                                     
 ccGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCTGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACg                                                                                                                                                 <  5:1318601/151‑1 (MQ=255)
   gtcgtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAATAAAGaaccg                                                                                                                                               >  7:1083457/1‑147 (MQ=255)
      gtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTTTATAAAAGCCGccttc                                                                                                                                            >  5:751067/1‑147 (MQ=255)
      gtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAAGCTTAc                                                                                                                                            >  4:1515556/1‑151 (MQ=255)
      gtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAATAAAgcaccatac                                                                                                                                            >  8:361988/1‑143 (MQ=21)
      gtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAATAAAGCAGCtttc                                                                                                                                            >  1:1339848/1‑149 (MQ=21)
         gCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCtt                                                                                                                                         <  2:822571/151‑1 (MQ=255)
              caAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTTTAATAAAGCGGCGTAccgcgac                                                                                                                                      >  5:57274/1‑144 (MQ=21)
               aaaTTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGCAGCTTACCTTGaaatt                                                                                                                                   >  4:603566/1‑149 (MQ=255)
                aaTTCTGAATGTATGGAATATGTTCGGCAATTTCAAAACCAATTTGTTGGTGTATCCCCAAGCGGTAAGGCACCTGATTCTGATTCCGGGATTCGGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACC‑TGTAAGTGc                                                                                                                                 <  2:683794/151‑1 (MQ=25)
                 aTTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGACGCTTACCTTGTAAGTGc                                                                                                                                 >  1:296/1‑151 (MQ=255)
                              cGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGt                                                                                                                    <  2:1188549/151‑1 (MQ=255)
                              ataaTTTGTTCCGCAAATTCAAAACCAATTAGTTGGTGTGTCGCGAAGGGTTTAGTGACGTGATTGTTTTTCATGCATTCCGTGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGt                                                                                                                    <  5:877943/149‑1 (MQ=255)
                                 atatGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGAAGCTTACCTGGTAAGTGAACCAAGTGGGGGTAt                                                                                                                  >  3:294734/1‑150 (MQ=255)
                                   atGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTATAAAAGAACCTTACCGAGAAATTTGACCCAGTTTGGGttcatg                                                                                                               >  3:495006/1‑146 (MQ=17)
                                   atGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGc                                                                                                               <  4:1402567/151‑1 (MQ=255)
                                   atGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGc                                                                                                               <  3:437845/151‑1 (MQ=255)
                                     gTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGcc                                                                                                              <  2:1037594/150‑1 (MQ=255)
                                             aaTTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATAAAAGAAGCTTACCTTGTAAGTGAACCCAGTTGGGGTATAGCCAAGCGGTa                                                                                                      >  7:1589740/1‑150 (MQ=21)
                                              agtCAAAACCAATTTGGTGGGGTATCGACAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTCACGCCGCCTATTTATTCAAGACGCGTCCATTGCAACTGCAACCAGTTGGGGTATCGCCAAGCGGTAAg                                                                                                    <  5:169864/149‑1 (MQ=21)
                                                  accgcccggttattgGGGTATCGCCAAGCGGTAAGGCACCGGATTGTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTgg                                                                                                                       >  3:1571650/13‑128 (MQ=21)
                                                  accgcccggttattgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTgg                                                                                                                       <  4:1573890/116‑1 (MQ=255)
                                                   aaaCCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCGTACGTGGTAAGTGCACCCAGTTGGCGTATCGACAAGCGGTAAGGCTcc                                                                                               >  7:49132/1‑151 (MQ=25)
                                                    cgcccggttattgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCtt                                                                                                                                              <  1:1106299/93‑1 (MQ=255)
                                                    cgcccggttattgGGGTATCGCCAAGCGGTAAGGCACCGGATTATGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCtt                                                                                                                                              >  2:1108009/11‑103 (MQ=17)
                                                      cccggttattgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGt                                                                                                                                   <  1:1093296/104‑1 (MQ=255)
                                                        cggttattgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCtt                                                                                                                                         >  1:57519/7‑104 (MQ=18)
                                                        actttGTCGGGGTATCGCCACGCGGTAAGGCACCGGATGCTGATTCCGGCAGGCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGAtt                                                                                          <  7:1347430/149‑1 (MQ=37)
                                                         aTTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGCACCTTTCCTGGTCAGTGCACCGAGTTGGGGTTTCGGCAAGCGGGAAGGCCCGGGAGTc                                                                                         >  7:1512591/1‑151 (MQ=18)
                                                           ttcgtgGGGTAGCGCCAAGCGGAACGGCTCCTGTTGTTCAGACGGGCCCTCCGAGGATCTAAGACTCGTACCACAGCAAATATATTCAAGCCGCTTCCCTTGCAAGTGCAGCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCt                                                                                        <  5:1453944/146‑1 (MQ=16)
                                                            tattgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCtt                                                                                                                                         >  2:195296/3‑100 (MQ=14)
                                                            ggatgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAATTTATTc                                                                                                                                                       >  4:1744227/4‑86 (MQ=11)
                                                             attgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATTCCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAACACGCTTCCCTTg                                                                                                                                        >  3:1131416/2‑100 (MQ=14)
                                                             attgGGGTATCGCCAAGCGGTAAGGCACCGGTTTATGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTg                                                                                                                                        <  4:1133086/99‑1 (MQ=255)
                                                              ttgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATTCCGGCATTCCCTGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACCCTTCCCGTGTAAGTGc                                                                                                                                 >  5:408583/1‑106 (MQ=17)
                                                               tgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAATAAAGAAGCAGATCTTTTGAGTGCACGGATTAGCGCAATCGCCAAGCGCGAAGGCACCGGATTCTAATTc                                                                                   >  7:706799/1‑151 (MQ=14)
                                                                   gTATCGCCAAGCGGTAAGGCACCGGTTTTTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCATTTTATTCAAGAGGGTTAGCTTGTAAGTGCACCCAGTTGGGGTATc                                                                                                                 >  7:293374/1‑117 (MQ=21)
                                                                   gTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATc                                                                                                                 <  8:293214/117‑1 (MQ=255)
                                                                      tCGCCAAGCGGTAAGGCACCGGTTTGTGATTCCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAg                                                                                                                           >  8:1424091/1‑104 (MQ=255)
                                                                      tCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCa                                                                                                                                <  5:808284/99‑1 (MQ=255)
                                                                      tCGCCAAGCGGTAAGGCACCGGATTATGATTCCGGCATTCCGAGGTTCGAATCCACGTACCCCAGCCAATTTATTCAAAACGATTACCATGTAAGTGCa                                                                                                                                >  6:808334/1‑99 (MQ=18)
                                                                       cGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGAAGCTTACCTTGTAAGTTCACCCAGTAGGGGTATCTCCAAGCGGTAAGGCAAAGGATTCTAATTcactcattc                                                                           >  4:894112/1‑143 (MQ=25)
                                                                            aGCGGTAAGGCACCGGTTTTTGATTCCGGCATTCCGTGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTg                                                                                                                                  <  1:709937/91‑1 (MQ=255)
                                                                            aGCGGTAAGGCACCGGTTTCTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACg                                                                                                                                                 <  3:370273/76‑1 (MQ=255)
                                                                            aGCGGTAAGGCACCGGATTGTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCGAGCGGt                                                                                                       <  3:1745422/118‑1 (MQ=255)
                                                                             gccggaatgaacatgATTCTGATTCAGGCATTACGAGCGTCGAATCCTCGAACTTCTGACTATATATTCAATTGGCTTAACTTGTAAGTGCACTCTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGt                                                                     <  5:717328/137‑1 (MQ=12)
                                                                                  aatgatcatgATTCTGTCTCAGGCTTTAAGAGGGTCGTATCCTCGTACTTCAGAAATTATATTATATACTCTTAAATTTCAATTGCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGa                                                                 <  4:1788146/141‑1 (MQ=2)
                                                                                     gCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGACAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATcc                                                             >  8:1008883/1‑151 (MQ=255)
                                                                                      cACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGt                                                                                                                                       >  5:1232676/1‑76 (MQ=11)
                                                                                      cACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGCACCGTACGTGGACAGTGAACCCAGTGTGCGTATCGACAAGCGCTAATGCGACGGTTTCTATTTCCGGCATTCCGAgtatctacac                                                              >  7:1793807/1‑140 (MQ=12)
                                                                                       aCCGGTTTTTGATTCCGGCATTCCGTGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCtt                                                                                                                                              <  8:1788118/68‑1 (MQ=21)
                                                                                           gATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGACAATTTATTCAAGACTCTTACCTTGAAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAGGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTAc                                                       <  7:85335/151‑1 (MQ=255)
                                                                                             ttCTTATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCa                                                                              <  6:336861/126‑1 (MQ=255)
                                                                                             ttCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCa                                                                              >  5:337334/1‑126 (MQ=255)
                                                                                              tCTGTTTCCGGGATTCCTAGGTTCGAATCCACGTACCCCAGCCATTTTATTATAGACGCTTCCCTTGTAAGTGCACCGAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTAccc                                                     <  7:671806/150‑1 (MQ=21)
                                                                                              ggggcgtCCGGGAGTCCGAGCGTCGAATCCTCCTACCCCAGCCACTATCTTCAAGACGCTTACCTTTTAACTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTAcccc                                                    <  7:1571126/145‑1 (MQ=18)
                                                                                                 gactCCGGCACTCCGACCGTCGAAGCCTCGCACCCCAGCCAATTTATTAAATACGCTTACATTGTAACAGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGc                                                 <  1:1417293/148‑1 (MQ=18)
                                                                                                  aTTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGAGGGGTACCTTGTGAGTGCACCGTGTTGGGGTATCGCCAAGCGGGAAGGCGCCGGAGTCTACTGCGGGCATTCCGAGGATCGTCGCCTCGTCCCCCCGcc                                                >  5:285312/1‑151 (MQ=21)
                                                                                                  aTTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAGGCGTTCCTGGTAAGTGAACCGAGTGTGGGTTGCTGCAAGCGGTAAGGCGCAGGAGTCTAGTTCATTCATTCATAGTATCGAGACATCGTCCCCCAGcc                                                >  1:780789/1‑151 (MQ=12)
                                                                                                   ttCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGcc                                                >  7:1032155/1‑150 (MQ=255)
                                                                                                      cggatttCAGAGGTTCGAATCCTCGTAATTCTGACAATTTATTCAAGTTTCTTAAATTGTAAGTGCAATTAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACAt                                            <  6:1752062/146‑1 (MQ=17)
                                                                                                         cATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTACTTCCGGCATTCAGAGGGTCGAATGCTCGTACCCCAGCCACATTa                                          >  1:1738878/1‑150 (MQ=255)
                                                                                                          ccgccccccggcGAAGCCACGTACCTCTGACAATCTATACAAGCCGCTGCCCTAGTAACCGCACCGAGTTGCGGTCTCTCCTCGCGTCAAGCGACCTGATTCTCATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTaaa                                        <  3:1414664/140‑1 (MQ=9)
                                                                                                          aTTCCGAGGTTCGAATCCTCGTACCCCAGCCACTTTATTAAAGCGCCGTACCGTGTAAGTGCACCGAGTTGGGGTATCGCCAAGCGGTAAGGCCCCGGAGTCTAGTTACGGCCTTCCGAGTTTCGAGTCCTCCTCCCCCCGCGACTTTaa                                         >  5:615072/1‑150 (MQ=19)
                                                                                                           ttCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGACGCTTACCGAGTACGTTATCCCATTTTGGGTATCGACAATCGCTAATGCACCGGATTCTAATTAAGGCATTCAGAGTATCGAAACCTCGTCCCCCAGCCACATTaaa                                        >  8:430985/1‑150 (MQ=255)
                                                                                                           cgccccccggcGAAGCCCCGTACCCCAGCCACTTTCTTCAAGCCGCGTCCCATGCAAGCGCACGCTGGGGGGGTATCGCCAAGCGGTAAGGCACCGGATGCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTaaaa                                       <  7:1012623/141‑1 (MQ=17)
                                                                                                              cgccggtCGAATCCTCGTAAGCCAGCCACTTTAGTCAAGCCGCGTCCATTGTAAGTCCAGACTGTTGGTGTATCGCCAAGGGTTAAGTCAGCGGATTCTTATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAg                                    <  7:34690/145‑1 (MQ=12)
                                                                                                               gAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCGCCGGATTCTACTTCCGGCATTCCGAGTTTCGACTCCTCGTCCCGCGGCCACATTAAAAAAGc                                   >  3:59073/1‑151 (MQ=37)
                                                                                                                aGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGGACCGAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCt                                  >  7:1647159/1‑151 (MQ=255)
                                                                                                                aGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCTCCAAGCTCTAAGGCACAGGATACTAATTCAAGCATTCCTAGGTTCGAATCCTCGTACCCCAGCAACATTAAAAAAGCt                                  >  4:1306013/1‑151 (MQ=37)
                                                                                                                aGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCt                                  >  5:516372/1‑151 (MQ=255)
                                                                                                                 ccgtCGAAGCCACGTACCCCAGCCAATTTATTCAAGCCGCTTACATTGTAACTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCTGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTc                                 <  7:62151/148‑1 (MQ=25)
                                                                                                                  gTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGACGCTTACCTTGTAAGTGAACCAAGTTGGGGTATCTACAAGCGGTAAGGCACAGTATTCTAATAAAGGCATTCCGAGTATCGAATCCTCGTACCCCAGCCAAATTAAAAAAGCt                                  >  8:1071117/1‑149 (MQ=25)
                                                                                                                  gTTAGAATCCTCTTATACCATACAATTTATTCAATACGCTTACATGCTTATTTTACGATCTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCg                                <  8:706070/151‑1 (MQ=17)
                                                                                                                   ttCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCA‑CCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGATCgat                              >  8:17839/1‑149 (MQ=255)
                                                                                                                    tCGAATCCTCGTACCCCAGCCAAATTATTAAAGAACCATACCTTGTAAGTGAACCAATTATGCGTATCGACAAGCGCTAAGGCACCGGATTCTAATTACGTCATTCAGAGTATCGAATCCACGTACCCCAGCAACATTAAAAAACCtatc                               >  4:1500576/1‑147 (MQ=255)
                                                                                                                     cGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCtt                             >  3:1398835/1‑151 (MQ=255)
                                                                                                                       aaTCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCg                           >  3:793667/1‑151 (MQ=255)
                                                                                                                        aTCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGTTTCTAATCATCCTACCCCAGCCACATTAAAAAACCTAGCTTCgg                          >  2:570810/1‑151 (MQ=37)
                                                                                                                          ccTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  2:1568786/1‑151 (MQ=255)
                                                                                                                              gTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCt                    >  4:1165281/1‑151 (MQ=255)
                                                                                                                              gTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCt                    >  6:1045328/1‑151 (MQ=255)
                                                                                                                               tACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCtt                   >  5:1754722/1‑151 (MQ=255)
                                                                                                                               tACCCCAGCCAATTTATTCAAGAAGCATACCTTGTAAGTGCACCCAGATGGCGTATCGCAAAGCGCTAAGCCACCGGATTCTAATTCAGGCATTCCGAGCTTCGAATCCTCGAACCCCAGCCACATTAAAAAAGCTAGCTTCGGCGAGCtt                   >  6:1449980/1‑151 (MQ=255)
                                                                                                                                aCCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttt                  >  8:671238/1‑151 (MQ=255)
                                                                                                                                aCCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttt                  >  6:1154712/1‑151 (MQ=255)
                                                                                                                                aCCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttt                  >  2:1392397/1‑151 (MQ=255)
                                                                                                                                  cccAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttttt                >  2:1546239/1‑151 (MQ=255)
                                                                                                                                  ccaaGCCAATTTATTAAAGAAGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATACTAATTCCGGCATTCCGAGGTTCGAATCCTAGTACCCCAGCAACATTAAAAAAGCTCGCTTCTGCGAGCttttt                >  2:1483672/4‑151 (MQ=25)
                                                                                                                                   ccAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTg               >  3:1444489/1‑151 (MQ=255)
                                                                                                                                    cAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCAGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGc              >  8:519216/1‑151 (MQ=255)
                                                                                                                                     aGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCt             >  3:1672495/1‑151 (MQ=255)
                                                                                                                                        cAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCtttt          >  6:921849/1‑151 (MQ=255)
                                                                                                                                         aaTTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTc         >  7:219499/1‑151 (MQ=255)
                                                                                                                                          aTTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCt        >  1:1071860/1‑151 (MQ=255)
                                                                                                                                            ttATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGc      >  5:1512391/1‑151 (MQ=255)
                                                                                                                                             tATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCg     >  8:1274077/1‑151 (MQ=255)
                                                                                                                                              aTTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGt    >  7:187088/1‑151 (MQ=255)
                                                                                                                                               ttCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCCGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGtt   >  6:612018/1‑151 (MQ=255)
                                                                                                                                                 cAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  1:1222989/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTC  >  DH1/3184440‑3184734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑