Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,169,7200CG25.9% 151.3 / 45.5 152E614Q (GAA→CAA) rsxCelectron transporter RsxC
Reads supporting (aligned to +/- strand):  ref base C (60/46);  new base G (33/4);  total (98/54)
Fisher's exact test for biased strand distribution p-value = 2.57e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

TGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCGCGGGC  >  DH1/2169573‑2169870
                                                                                                                                                    |                                                                                                                                                        
tGGCGCGGGCAATAGCCGCGTCCACTGCGGCTTTGCGCGTATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGGTGCTGTTCCAGCTTGCGTGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACC‑TGttct‑t                                                                                                                                                       <  3:248508/150‑1 (MQ=14)
 ggCTCGGGCAATAGCGGCTGCTACTGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCGTTCGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACC‑TGTtct‑tct                                                                                                                                                     <  5:1245444/151‑1 (MQ=14)
   cccGGGCAATAGCCGCGGCGACTGCGGCGTTCCGCTTAGCTACCTGCTCTTCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGGTTGCGGGCTTTCGCTCGGGCAATAGCGGCTGCGACGGCGGCTTTGCGCGGATCGACC‑TGTTCT‑TCTgg                                                                                                                                                   <  6:1546191/149‑1 (MQ=11)
     cGGGCAATAGCGGCGTCTACTGCCGCTTTGCTCGGTTGTACGTGTTGTTCTGGCTCGTCATTAGCCTGTTGCTGTTCGTGGTTGTTTGCTTTTGCTCGGGCAATAGCGGCTTCTACGGCGGCTTTGCGCGGATCGACC‑TGTTCT‑TCTGGtt                                                                                                                                                 <  8:1354805/151‑1 (MQ=14)
       gtcattAGGGGCTTCTACTGCTGCTTTTCGCTGATCTAGCTGTTGTTCTTGGTCGGGATTAGCATGTTGCTGTTCGAGCTTGGGTGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACC‑TGTTCT‑TCTGGt                                                                                                                                                  <  8:686957/143‑1 (MQ=14)
        gCAATAGCGGCTGCCTCGCGGTCTTTGCTCGGATCTACCTGTTGTTCTGGCTCGTCATTAGCCTTTTGCTGTTCCAGGTTGCGTGCTTTCGCTCGGGCAATAGCGGCTTCTACGGCGGCTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCg                                                                                                                                              <  3:1117695/151‑1 (MQ=14)
         cAATATCTGCTTCTACGGCCTCTTTGCGCGGATCCACGTGTTGTTCTGGATCTGCATTAGCCTGGTGCTGTTCGTGGTTGCGGGCTTTTGCGCGGGCAATAGCGGCTTCTACTGCGGCTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGc                                                                                                                                             <  2:1373512/151‑1 (MQ=11)
            aaGCGGCTTCTTCTGCTGCTTTGCGCGGAGCGACCTGTTGTTGTGGCTGAGCTTGTGCCGGTTGGTGTTCCCGGTTTCGGCGTTTGGCTCGGGCAATAGCCGCTTCGACTGCGGCTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGCAtt                                                                                                                                          <  3:884503/150‑1 (MQ=2)
                 gctttaactgatgcTTTGTTCGGATCCACGTGTTGTTTTGGATCTTTATTATCCTTTTGCTGTTCGTGTTTTCTGGCTTTTGCTCGGGCAATAGCGGCTTCTACTGCGGCTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGCATTa                                                                                                                                         <  8:1727887/135‑1 (MQ=2)
                            ggcTTTGCGCGGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGTCAATAGCTGCTTCGACGGCTGCTTTCCGGGGATCAACA‑AGTGGT‑TCTGGGTCCTGATTTTCCTGATGATCTTcc                                                                                                                          >  6:1288384/1‑151 (MQ=11)
                              cTTTGCGCGGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGATTTACGAGGATCACAA‑AGTTAT‑GCTGCTTCCGCATTTTCCTGTAGATGTTcc                                                                                                                          >  6:1029866/1‑149 (MQ=14)
                                  tcgtttttttaattgttgtTCTTGCTCGTTTTTATCCTTTTGCTGTTCGGGTTTTTTGGCTTTTGCGCGGGCAATAGCGGCTTCTACTGCGGCTTTGCGCGGATCTACC‑TGTTGT‑TCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCtt                                                                                                                     <  2:1068786/137‑1 (MQ=2)
                                   cgcgGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGAGCTTTGGCACGGGCAATAGCTGCATCAACGGCAGATTTACGAGGATCAGCA‑AGTTTT‑TCTGCTTCGGAATTATGCTGATGATCTTcctgattcc                                                                                                                   >  4:737582/1‑144 (MQ=2)
                                     cgGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTACGTCCTTTGGCACTGGAAATAGCGTATTCGAATTAGTGTTTGCTAGGATAGACC‑TGTAGT‑TCTATTTAATAATTAGCCTGTTGCTGTTCCAGCTTg‑cgg                                                                                                                >  8:1023007/1‑150 (MQ=2)
                                       gATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCTGCTTCGACGGCGGCTTTCCGCGGATCGACA‑AGGTGT‑GCTGGGTCCGCAGTATCCTGATGCTCTTCCAGCttcccacc                                                                                                               >  1:1080580/1‑145 (MQ=11)
                                       gATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGAGGATCGACA‑AGTTGT‑TCTGGTTCCGCAATACCCAGATGATGTAACAGCATg‑cccgc                                                                                                              >  5:1750874/1‑151 (MQ=11)
                                           gACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCTGCTTCGACGGCAGCTTTGCGGGGATCGGCA‑TCTTTT‑GCTGCTTCCTAATTATCCTGATGATCTTcctgattccgacagttt                                                                                                           >  8:325648/1‑136 (MQ=9)
                                            aCCTGTTGTTCTCGCTCGGCATTAGCCGGGTGCTGTGCCAGCTTGCGCGCTTGGGCACGGGCAAGATCGTCCGCGCGGGCCGCTTTGCGCGGATCTACC‑TGTTCT‑TCTGGGTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTgg                                                                                                         <  7:64010/151‑1 (MQ=11)
                                             ccTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCTGCTTCGACGGCAGGTTTACGGGGATCAACA‑AGGTGT‑TCTGGTTCGTGAGTATCCTGATGATCTTCCAGCttcccactgttttg                                                                                                         >  4:1448257/1‑139 (MQ=2)
                                             ccTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGAGCTTTGGCGCGGGCAATAGCTGCTTCGACGGCGGCTTTCCGCGGATCAACC‑ACTGGT‑TATGCTTCCGAAGTATGCTGATGTTGTTCCAGGTTg‑ccactgttttc                                                                                                        >  7:1226388/1‑141 (MQ=2)
                                              cTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGAGCTTTGGCCCGGGCAATACAGCCGACGCCGCCGGCTTTCAGCCGATCGCCA‑TCTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTACAGCTTG‑CGCGCTTTGGcg                                                                                                       >  7:992424/1‑150 (MQ=11)
                                               tgttgtTCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCGATAGCTGCTTCAACGGCGGATTTGCGAGGATCGGCA‑TGGTGT‑TCTGGGTCCTGATTATCCTGAAGGTCTTCCAGAttcccggaatatccgc                                                                                                       >  5:644573/1‑137 (MQ=2)
                                                 ttgttCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCCCGGGCAATAGCTGCTTCGACGGCGGGTTTGCGCGGATCGCCC‑TGTTGT‑TCTGGTTCGGGATTTGCCTGTTGATGTTCCTGGTTG‑AGCTCGTTTGCGCgg                                                                                                    >  7:823237/1‑151 (MQ=11)
                                                  tgttCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCTGCTTCGACGGCGGGTTTGCGCGGATCGGCA‑TGGTTT‑TCTGGGTCGGGATTATCCTGTTGGTGTTCCAGGTTg‑cgtctgttttgcggtt                                                                                                   >  1:638735/1‑137 (MQ=11)
                                                       tGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCGCGGGCAATAGCGGCTTCGACGGCGGGTTTGCGGGGATCGGCA‑TGTGGT‑GCTGGTTCCGGAGTAGGCTGTTGGTGTTCCTGGTTGCCGCGTTTTGGC‑CGGTCAAtg                                                                                              >  1:1432487/1‑150 (MQ=2)
                                                       tGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCGCGGGCAATAGCGGCTTCGACGACGGCGGTGCCCGGCTCGACG‑GGAGGG‑GAGTGTGGCGGGTTAGGCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACgggggcgg                                                                                              >  3:788816/1‑146 (MQ=2)
                                                       tGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACC‑TGTTGT‑TCTGGTTCCGGATTATCCTGTTGGTGTTCCAGCTTG‑CGGGGTGTGGCCCggtgaatg                                                                                              >  7:972278/1‑145 (MQ=11)
                                                       gggCTCGGCATTAGCCTGTCGCTGTTCCAGCCCGCGGGCTCTCGGGAGTGAAAGCCCGGCAGCGCGGGCCGCTTCCGCCGTATCAACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATa                                                                                              <  7:89606/150‑1 (MQ=9)
                                                         gCTCCGCATTAGCCTGTTGCTGTTCCAGATTGCGTGCTTTGGCTCGGGCAATAGCCGCTGCGACGGCTGCTGTGCGGTGAGCTCGC‑GGGTGG‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGc                                                                                            <  7:1623340/151‑1 (MQ=11)
                                                         gCTCCGCAGTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACC‑TGGTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGc                                                                                            <  7:724119/151‑1 (MQ=255)
                                                          cTCGGCATTAGCCTGCTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGGTTTGCGGGGATCGGCC‑TCGGGT‑TCTGGTTCCGCAGTAGCCTGTTGGTGTTCCAGCTTG‑CGCGCGTTgtccgggggactcgct                                                                                           >  3:51600/1‑136 (MQ=11)
                                                             ggCTTTTGCGTGCTGCTGTTCCAGCTTGCGTGGTTTTGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCGTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCt                                                                                        <  8:393278/151‑1 (MQ=14)
                                                              cccctctcccgatccTGTTCCAGCTTGCGTGCTTTTTTTTTTTCAATAGCGGCTTCGACGTCTGCTTTTCTATGATCAACC‑TTTTTT‑TCTTTCTCGTCATTATTCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCtt                                                                                       <  2:864417/137‑1 (MQ=2)
                                                                    gCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCTGCTTCGACGGCGGGTTTGCGGGGATCGACC‑TGTTGT‑TCTGGTTCCGGATTATCCTGTTGGTGTTCCAGGTTG‑CGCCGTTTGGCACGTGAAATAGCTGCTTctccgg                                                                                 >  7:510485/1‑146 (MQ=11)
                                                                       tGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGGTTTGCGCGGATCGACC‑TGTGGT‑TCTGGTTCGGGATTATCCTGATGCTGTTCCAGATTG‑CGAGGTTTGTCCGGGGCAATATCGGATTCGAcggcgg                                                                              >  3:1571974/1‑151 (MQ=11)
                                                                         ttGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGGTTTGCGCGGATCGGCA‑TGTTGT‑TCTGGTTCCGCATTAGCCTGGTGCTGGTCCAGCTTG‑CGCGGGTTGGCTCGCGCCAGAGCGGCTTCGAcggctggt                                                                            >  3:137781/1‑147 (MQ=11)
                                                                          gccgggcccAGCTGGCGTGGTTTTTCACTGGCAATAGCGGCGTCGACGGCGGCTTGTCG‑GGCTTGACCGTCTTCC‑TGTGGTGGGGCATTAGGATGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCtt                                                                           <  1:466245/144‑1 (MQ=9)
                                                                             tGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGCCC‑TGTTGT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCCCGGGCAATAGCTGCTTAGAcggcaggttgac                                                                        >  5:1796829/1‑143 (MQ=11)
                                                                               gtCCAGCTTGCGTTCTTTTTCACGCTCAATAGCGGCTTCGAAGGCTGCTTTTCGCTGATCGACC‑TGTTCT‑TCAGGTTCCGCATTAGACTTTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTgcgc                                                                      <  4:1515592/150‑1 (MQ=14)
                                                                                 ccAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGGGGATCGCCC‑AGGTGT‑GCTGGTTCCGGATTAGCCTGTTGCTGTTCCAGCTTG‑CGAGGTTTGGGCCCGGGAATAGCTGCTTCTCCGGCTGGTTTGcgcgc                                                                    >  3:447660/1‑150 (MQ=11)
                                                                                       tGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGCCC‑TGTTGT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGGTTTTCGAGGATcatc                                                              >  7:673596/1‑148 (MQ=14)
                                                                                        gCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGGTTTGCGGGGATCGGCA‑TGTTGT‑TCTGGTTCGGGATTATCCTGTTGGTGTTCCAGGTTG‑CGGGATTTGGGCCGTTGAATCGCATCTTAAACGGCCGCTTTTCGAGGAtagtc                                                              >  1:1102119/1‑146 (MQ=0)
                                                                                          gTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGGTTTGCGCGGATCGCCA‑TGTTGT‑TCTGGTTCGTGAGTTTCCTGTTGGTGTTCCAGCTTG‑CGGGGGTGGGGCGGGGGAATAGCTGCTTCAGCGGACGCTTGTCGCggcgagtcacg                                                           >  7:806426/1‑142 (MQ=2)
                                                                                            tgtttGTCACGTGCAATTGCGGCTTCGCCGGGGGGGAGGCGCGGCTGGAGG‑TGTGAG‑TCGGGCTCCGCATTTCCCTGTTGCTGTTCCCGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCtgt                                                          <  3:62515/148‑1 (MQ=2)
                                                                                            gCTTTGGCCCGGGCAATAGCCGCTTCGCCGGAGTGTCTG‑GCGTTCAGACG‑CGGGCTCTCCGGATCTGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCGCGGGCGATAGCTGCTTCAACGGCAGTTTTACGTGGATcagcaacgg                                                         <  1:1494107/151‑9 (MQ=12)
                                                                                            gCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTCTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCtgtt                                                         >  4:1203895/1‑151 (MQ=255)
                                                                                              tttGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGCCC‑TGTTCT‑TCTGGTTCCGCATTTGCCTGTTGCTGTTCCAGCTTG‑CCCGCTTTGGCGCGGGCAATAGCTGCTTCGCCGGCAGCTATGCGAGGATCGTCCtcgctt                                                       >  5:998188/1‑146 (MQ=11)
                                                                                              tttGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACC‑TGTTGT‑TCTGGTTCCGCATTAGCCTGTTGGTGTTCCAGCTTG‑CGCGCTTTGGCCCGGGCAATAGCGGCTTCTACGGCGGGTTTGCGAGGATCgtcaaggggt                                                       >  5:327928/1‑142 (MQ=11)
                                                                                              gttGGCGCGGGCAATAGCCGCGTCCACGGCGGCTGGGGGCGGAGCGCCG‑TGCTGT‑GCTGGGCCCTGAGTAGCGTGGTGCTGGGCCCGCTTG‑CGGGCCTTGGCCCGGGCAATAGCCGCTTCGACTGCGGCTTTGCGCGGATCGACCtgttgt                                                       <  3:687295/150‑1 (MQ=2)
                                                                                                     cGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACC‑TGGTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCCCCGGCAATAGCTGCTTCTACGGCAGGTTTGCGAGGCTCTCCCTGGGGTTCTgcgt                                                >  4:206441/1‑148 (MQ=11)
                                                                                                      gggCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGGTGTTCCAGCTTG‑CGCGCTTTGGCCCGGGCAATAGCTGCTTCTACGGCGGGTTTGCGGGGATCTCCCTGTGGTTCTGCTTc                                               >  1:338029/1‑151 (MQ=14)
                                                                                                        gCAATAGCCGCTTCGACGGCTGCTTTGCGCGGATCTACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGAcg                                                                                  <  2:194904/114‑1 (MQ=255)
                                                                                                           aTAGCTGCTTCTACGTCTTCTTTGCGCGGATTGACT‑TGTTTT‑TCTGGTTCGTTATTAGCCTGTTGCTGTGCGTGTTTG‑CGGGCTTTGGCTCGGGCAATAGCGGCTTCTACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCa                                           <  4:591170/150‑1 (MQ=11)
                                                                                                            tAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACC‑TGTTGT‑TCTGGGTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCCCGGGCAATAGCTGCTTCTCCGGCAGTTTTTCGAGGATCAGCCTCTGATGCTGGTTCGGGAtt                                         >  2:1216103/1‑151 (MQ=14)
                                                                                                            acgcccccgccaCGGCCGCTTTGCTCGGATCCACC‑TGGTCT‑TCTGGCCCCGCATTACCGTGTTGCTGGAGCTGATTC‑CTGGCGTTTTCCCATGAAATAGCCGCTGCAACTGCCGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCAtt                                         <  1:996927/140‑1 (MQ=2)
                                                                                                             aGCGGCTTCGACGGCGGCTTTGCGCGGATCGCCC‑TGTTGT‑TCTGGTTCCGCATTATCCTGTTGCTGTTCCAGCTTG‑CGCGATTTGGAACGGGCAATAGCGGCTTCTACGGCAGATTTGCGAGGATCAACAAGTTGTTCTGGGTcctaatt                                         >  6:485355/1‑145 (MQ=11)
                                                                                                              ggcgctgagACGGCTGCTCTGCCGGGCTCTACC‑TGTTCT‑TCTGGTTCCGGATTAGCCTGTTGCTGTTCCAGCTTG‑CGTGCTTTGTCTGGTGCCATAGCGGCTTCGACGGCTGCTTTTCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAg                                       <  5:423168/143‑1 (MQ=11)
                                                                                                              gCGGCTTCGACGGCGGCTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCGCGGGCGATAGCGGCTTCGACGGCTGGTTTGCGCGGATCGACATGTTGTGCTGGTTCCGGAtt                                         >  5:1496041/1‑149 (MQ=14)
                                                                                                              cccgCTTCCCCGCCGGCTTTGCGCGGATCGTCT‑TTTTTT‑TATTGATACGGATTAGACTGTTTTTTTTACATATTT‑ATAGATTTTTTACGTTCAATAGGGGCTTCGTATGTGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAg                                       <  2:486833/148‑1 (MQ=2)
                                                                                                               cGGCTTCGACGGCGGCTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCGCGGGCAATAGCTGCTTCGACGGCGGGTTTGCGGGGATCCCCCTGGTGTTCTGGGTCCttattatc                                      >  5:1104073/1‑149 (MQ=14)
                                                                                                                ggCTTCGACGGCGGCTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCGATAGCTGCTTCGACGGCGGGTTTTCGAGGATCGACCACGGGTGCTGGTTcctgattttcc                                     >  5:1701264/1‑142 (MQ=11)
                                                                                                                     cGACGGCGGCTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCGCGGGCAATAGCTGCTTCGACGGCAGATTTACGAGGATCACCAAGTTTTTCTGCTTCCTAATTATCCTga                                  >  6:683200/1‑148 (MQ=11)
                                                                                                                      gACGGCGGCTTTGCGCGGATCGACC‑TGTTGT‑TCTGGCTCGGCATTAGCCTGTTCCTGTTCCAGCTTG‑CGAGCTTTGGCACGGGCAATAGCTGCTTCTACGGCAGCTTTACGAGGATCGACAACGGGTTCTGCGTCCGAATTATCCTGATgg                               >  2:1729139/1‑150 (MQ=11)
                                                                                                                       aCGGCAGCTTTGCGCGGATCAACC‑TGTTCT‑TCTGGATCCGCATTAGCCTGTTTCATATCAAGATAG‑ACCGCTTTGGAAAGGGAAATAGCGGATTAGAAGCAGGCTTTGCTCGGATCGACATGTTGTTATGGTTCCGCATTAGCCTGtttgt                              >  8:28866/1‑148 (MQ=2)
                                                                                                                        cggcggCTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCGCGGGCAATAGCTGCTTCAACGGCAGGTTTCCGGGGATCGCCCAGTTTTGCTGGTTCCTGATTAGCCTGTTGCTg                             >  2:709214/1‑151 (MQ=14)
                                                                                                                         ggctgattttatatgATCCACC‑TCCTCC‑TCTGTCTCCGAATAAGCCTCTTCCTGTTCCCGTTTT‑CGAGGCTCTTCACTGGTAATAGCGGCTTCGACGGCTGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGt                            <  8:989665/137‑1 (MQ=2)
                                                                                                                         cgccgcTGTGCGCGGATCTATT‑TTTTCT‑TATGTTTCGGCTTTATCCGGTTGCTGTTCCATTTTT‑AGCGCTTTGTCACTTGCAATAGCGGCTTCGACGGCTGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGt                            <  2:619825/147‑1 (MQ=2)
                                                                                                                            ggcTTTGCGCGGATCGACC‑TGTTGT‑TCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGTGCTTTGGCACGGGCAATAGCTGCTTCGACGGCGGGTTTGCGGGGATCGGCAAGGGGTTCTGGGTCGTGAGTTTCCTGTTGATGTTcc                         >  8:826765/1‑151 (MQ=14)
                                                                                                                            ggcTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCTGCTTCGACGGCGGGTTTACGCGGATCTCCCTCTTGTTCTGCTTCCGCAGTTGCCTGTTGGTCTTcc                         >  3:1664596/1‑151 (MQ=14)
                                                                                                                             gcTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCTGCTTCGACGGCGGATTTGCGCGGATCCACCAGTGTTTCTGCTTCCTGATTAGCCTGATGATGTTcc                         >  4:476341/1‑150 (MQ=14)
                                                                                                                             gcTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTcc                         >  7:723952/1‑150 (MQ=40)
                                                                                                                              cTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCGCGGGCGATAGCGGCTTCGACGGCGGGTTTGCGAGGATCGACCTGGTGTTCTGCTTCCGGAGTATCCTGTAGCTGTGcc                         >  2:214635/1‑149 (MQ=14)
                                                                                                                                 tGCGCGGATCGACC‑TGTTCT‑TCTGGCTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCt                     <  7:108027/150‑1 (MQ=25)
                                                                                                                                 cgcgcGGAGCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGTGCGTTGGCACTGGCAATAGCGGCTTCGTCGGCTGCTTTGCTCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCtt                    <  7:1024084/150‑1 (MQ=11)
                                                                                                                                 cccgcgGATCGACC‑GGTTCT‑TCTGGATCCGCATTAGCCCGTTGCTGCCCCAGCGTG‑CGGGATCTGCGTCTCTCAATTGCAGATTGGAGCGCGTCCTTTCTCGGATCTACCTGTTGTTCTGGCTCCGCATTAGCCTGTTGCTGTTCCAGCtt                    <  7:1319690/149‑1 (MQ=2)
                                                                                                                                  gcgcGGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCCCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGCCCTCTTGTTCTGCTTCCGGATTAGCCTGTTGCTGTTCCAGGTTg                   >  1:799243/1‑151 (MQ=14)
                                                                                                                                   cgcgGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCGCGGGCAATAGCTGCTTCGACGGCTGCTTTACGCGGATCGCCCTCGGTTTCTGCGTCGGGAGTAGCCTGTTGATGTTCCAGCttcc                  >  8:1671543/1‑149 (MQ=14)
                                                                                                                                    gcgGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGCCCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTgcc                 >  8:378502/1‑150 (MQ=255)
                                                                                                                                    gcgGATCAACC‑TGTTCT‑TCTGGTACCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGTGCTTTGGCTCGTGCAATAGCGGCTTCGACGGCGGCTATTACCGAATAGACATGTTGATCTGGATAGGCATTAGAATATTGATGATCCAGTTTgcg                 >  6:43455/1‑151 (MQ=2)
                                                                                                                                     cgGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCCCGGGCAATAGCTGCTTCGACGGCGGGTTTGCGAGGATCGCCATGTTTTTCTGGTTCCTAATTATGCTGTAGTTGATCCAGATTccgc                >  3:1218648/1‑151 (MQ=14)
                                                                                                                                     cgGATCAACC‑TGTTCT‑TCTGGATCCGCATTAGCCTGTTGCTGTTCCAGATTA‑CATCCTTTGACACTCGAAATAGCGAATTCTAATAAGACTTTTCTAGGATAGACATGTAGTTCTATAAATAAATTATACTGTTGCTGTTCCAGCTTgcgg                >  8:1318591/1‑150 (MQ=2)
                                                                                                                                      cgcacccccctcggct‑tctGGCTCCGCATTAGCCTGTTGCTGTTCCCGCTTG‑CGCGCTATGCCCCGGCGTTTAGCGTCTTCCCCGGCGGGCTCTCGCGTTGCCACCTGTTCTTCTGGCTCCGCATTAGCCTGTTGCTGTTCCAGCTTgcgc                <  5:501813/137‑1 (MQ=2)
                                                                                                                                       gATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCCACGGCGGCTTTACGAGGATCGCCAACGTTTGCTGCTTCCGCAGTATCCTGTTGGTGTTCCAGATTgcggcg              >  6:795277/1‑148 (MQ=14)
                                                                                                                                         tCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCGCGGGCGATAGCGGCTTCGACGGCGGGTTTGCGCGGATCGCCCAGTTGTGCTGGGTCCGGAGTATCCTGAAGGTCTTCCAGATTccgagggt            >  3:1272102/1‑146 (MQ=14)
                                                                                                                                         tCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTACGCGGATCGCCATCTTTTTCTGGTTCCTCATTATCCTGTTGCTGTTCCTGCTTgcggcgtt            >  2:1474973/1‑146 (MQ=14)
                                                                                                                                          cGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCCACGGCGGCTTTGCGCGGATCGACATCTTGTTCTGCTTCCGCATTAGCCTGATGCTGTTCCAGATTGCGCGGttt           >  3:1008375/1‑151 (MQ=14)
                                                                                                                                           gACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCCCGGGCGATAGCTGCTTCCACGGCCGGTTTGCGGGGATCCACATGTGGTTCTGGTTCCGCATTATCCTGTTGGTGTTCCAGCTTgcgggagttt          >  3:1252775/1‑144 (MQ=14)
                                                                                                                                           gACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGCCCTGTTGTGCTGGTTCCGCATTAGCCTGTTGCTGTTCCTGCTTccgaggtt            >  4:161323/1‑144 (MQ=14)
                                                                                                                                           gACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGGTTTg          >  7:572366/1‑151 (MQ=255)
                                                                                                                                           gACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGAtttt          >  3:395476/1‑150 (MQ=255)
                                                                                                                                           caCC‑TGTTCT‑TCTGGCTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCtt                                                                           <  3:1643337/85‑1 (MQ=255)
                                                                                                                                             cc‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCTGCTTCGACGGCAGGTTTACGTGGATCTACATGTTGTGCTGCTTCCGAATTAGCCTGTAGCTGATCAAGCTTGCACGCTATgg         >  4:1185795/1‑150 (MQ=14)
                                                                                                                                             cc‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGGCCTGTTGTTCTGGTTCCGGATTATCCTGTTGCTGTTCCAGCTTGCGCGCTTTGgc        >  5:1258291/1‑151 (MQ=255)
                                                                                                                                              c‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTACGCGGATCGCCCACGTGTGCTGGTTCCGCATTATCCTGGTGGTGTTCCAGCTTccgccggtgggcc       >  4:756575/1‑142 (MQ=14)
                                                                                                                                                 gTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTAGATCGGAAGATAGCCCGTCAGAACTCGCCTGACACCGGAGCATCGGGTTGTTCTGGTTCCGGATTAGCCTATTGATGTTCCCGCGTGCGGGGGTTGGggcg     >  7:1246106/1‑151 (MQ=2)
                                                                                                                                                 gTTCT‑TCTGGTACCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGGGCTTTGGCACGGGCAAt                                                                                               >  1:667575/1‑61 (MQ=255)
                                                                                                                                                  ttct‑tcTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCGCgg    >  5:719568/1‑151 (MQ=255)
                                                                                                                                                    ct‑tctGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGGAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGCCCTGTTGTTCTGGTTCCGCATTAGCCTGTTGGTGTTCCAGCTTGCGCCCTTTTGCGCGTGc  >  1:372907/1‑151 (MQ=14)
                                                                                                                                                    ct‑tctGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCTGCTTCGACGGCGGCTTTGCGAGGATCGACATCTTGTTCTGGTTCCGAATTAGCCTGTAGATGTTCCAGATTGCCAGCTTAGTcgcgtg   >  6:1104662/1‑148 (MQ=14)
                                                                                                                                                    ct‑tctGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGGTTTGCGAGGATCGCCCTGTTGTTCTGGTTCCGCATTATCCTGTTGCTGTTCCAGCTTGCGCGCTTTTGCGCggtc  >  3:976201/1‑149 (MQ=14)
                                                                                                                                                    |                                                                                                                                                        
TGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACC‑TGTTCT‑TCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTG‑CGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCGCGGGC  >  DH1/2169573‑2169870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑