Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,065,6050AT25.6% 330.3 / 110.0 220intergenic (+40/‑23)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (94/69);  new base T (37/19);  total (131/89)
Fisher's exact test for biased strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

CAAAAAATCTGCAGTAAAGTAAGTTTCCCGAT‑GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTA  >  DH1/1065457‑1065754
                                                                                                                                                          |                                                                                                                                                      
gcaatagacTGCAGTAGAGTAAGCTT‑CCGATGGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATcagc                                                                                                                                                      <  5:527562/143‑1 (MQ=18)
    aaaTCTGCAGTAAAGTAAGTTTCCCGAT‑GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATcaagagc                                                                                                                                                   >  3:460017/1‑145 (MQ=255)
       tCTGCAGTAAAGTAAGTTTCCCGAT‑GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATcagatcgcaat                                                                                                                                               >  3:315253/1‑143 (MQ=255)
         cgCAGTAAACTAAGTTTCCCGAT‑CCATCTGTATTTCAGCTGGATAGAGTACTGCGATACTAAC‑AT‑AGCTTTAGTATGTTCGAATCCTCCACGATGTACCTTTTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAaaata                                                                                                                                             <  2:1229978/150‑1 (MQ=16)
               aaagtaaGTTTCCCGAT‑GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCGGTAGTAGCGGTAGC‑‑A‑ATATcagcagagatctaaagctc                                                                                                                                       >  7:524169/1‑138 (MQ=18)
                 gcttcaccgcccTAT‑GCATCCGGAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTccc                                                                                                                                    <  7:1061732/142‑1 (MQ=255)
                 agtaagtTTCCCGAT‑GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTAATTCATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAaaataactga                                                                                                                                        >  8:1306355/1‑144 (MQ=255)
                 agtaagtTGCCCTTT‑GAATAGGTAGCTTAGCTGTATAGACTACTCGGCT‑CGTAT‑CGCTTGGGGCTTTGGTTGTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTccc                                                                                                                                    <  8:630293/151‑1 (MQ=9)
                    aagtaccgctct‑GCATCGGTAGCTCAGCTGGACAGACTAATCGGCTACCACCGCT‑AG‑GCGCGCAGCTTGGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGa                                                                                                                                  <  3:886192/139‑1 (MQ=12)
                     ctcagccattt‑GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGa                                                                                                                                  <  8:548860/139‑1 (MQ=255)
                     caccgccattt‑GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATg                                                                                                                                <  1:752139/141‑1 (MQ=255)
                         gcctgccgatctCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCAt                                                                                                                             <  5:504530/140‑1 (MQ=255)
                          cgcGAT‑GCATCCGTAGGTCAGCTTGATAGAGTTCTCGGCCACGATC‑CG‑TTCGGTCTGAGGTTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATcc                                                                                                                           <  7:1012662/149‑1 (MQ=21)
                          ccccct‑GCATCCGTAGCTCAGCGGGCTAGACTAATCGGCTAGGAAG‑CG‑TAGGCTCGGAGGGTGTAATCCTCCCGGATGCACCATCTCTTAATTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAGAATAAGTTTTCCCGATGCATc                                                                                                                            <  7:1326593/146‑1 (MQ=2)
                          cccGAT‑GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTATTAATTGATATGGCTTTAAGAACGGAAGC‑‑A‑AGAGCTGGAGTAAAATAA‑ATTTcacaatactga                                                                                                                            >  2:916769/1‑140 (MQ=17)
                          cccGAT‑GCATCCGTACCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGCATGCACCATCTCTTACTTGATATGGCTTTAGTAGAGGTAAC‑‑A‑ATATCAACATAAaaataa‑agtacacaatccaaa                                                                                                                            >  8:405897/1‑136 (MQ=18)
                                  catcggtatgaaaGCTGGATAGAGTACTCGGCGACGATC‑GC‑TGCGCTCGGAGCGTCTAATCCTCCCGGATTCACCATCTCTTACTTTATATGGCTTTAGTAGCGGTATA‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTc                                                                                                                    <  5:829725/139‑1 (MQ=17)
                                     ccGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACTTGATATCGCATTAGAAGCTGTATA‑‑A‑AAATAAGCAGTAAAATAA‑AATTCCCGATGCaaagatagcatt                                                                                                                   >  6:504522/1‑138 (MQ=17)
                                         agctgtgCTCGATAGAGTTCTCGGCGACGAGCGCG‑AG‑GCTCGGCGCGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGa                                                                                                             <  1:297410/145‑1 (MQ=14)
                                         aGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTAGTCCGTAATATGCATCAAGTAGCGTTAGG‑‑A‑ATATCACGAGGAAAAAAA‑ATGACCCGATTCCTACGTAAATCAGcggtt                                                                                                             >  7:12903/1‑147 (MQ=2)
                                          gCTCAGCTGGATAGAGTACTCGGCTACCAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATCCCACAAGACGTACTAGATAAGGCAGTA‑AATCCGGATA‑‑ATAGATCAGCAGTAAAATAA‑ATTTCCCCATGAATACGTAGCTCAGCTgg                                                                                                              >  7:1206389/1‑149 (MQ=9)
                                             cAGCTGGATAGAGTACTCGGCTACGAAC‑CG‑TGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTgg                                                                                                              <  2:1380621/146‑1 (MQ=255)
                                              aGCTGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGATTTAGTAGCGGTAGC‑‑A‑AGATGAGCAGGAAAATAA‑ATTTCCCGCTGCGGCCGTAGCTCAGGTGGATag                                                                                                          >  7:54193/1‑149 (MQ=25)
                                                 tGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCGTACTTCATATGCCGATAGGACGGGTATC‑‑A‑AGAGCAGCAGGAAAATAC‑ATTTCGCGCTGCGTCCGTAGCGCTGCTGTATAGAGtcg                                                                                                     >  1:852428/1‑149 (MQ=17)
                                                 tGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTATTACTTGATATGGCGTTAGTAGCGGTATC‑‑A‑ATATCACCAGTAATATAA‑ATTTCCCGATGCATCCGTAGCTCAGCGGGATAGAGTAc                                                                                                     >  7:689894/1‑151 (MQ=255)
                                                 tGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATCCTAAATCTTGATAAATTAGCTGTATA‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCAGATGAGTCAGTAGCTCAGCTGGATAGAGt                                                                                                       >  5:93927/1‑149 (MQ=17)
                                                   gATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAGAATAA‑Gtttt                                                                                                                                      >  3:643900/1‑115 (MQ=255)
                                                          tACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCGAGATATTGATATAGGAAGAGAAGC‑‑C‑ATATCAGCAGGAGAAAAT‑AATTAGAGAGGCCTCCGTAGCTCAGCTGGATAGAGTACTCGGCTACg                                                                                            >  1:541228/1‑151 (MQ=9)
                                                             tCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTagaa                                                                                                                                               >  5:744637/1‑98 (MQ=255)
                                                               cgCTACGACC‑CC‑CGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCg                                                                                       <  1:1261344/150‑1 (MQ=255)
                                                                 cTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTag                                                                                                                                                 >  3:1393627/1‑91 (MQ=255)
                                                                 cTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTAGTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCGTACGTAGACCAGCTGGATAGAGTACACGGCTCCGAAACgcc                                                                                     >  5:1781885/1‑149 (MQ=18)
                                                                     attacgttgtcGGTCTGAGGTTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGc                                                                                    <  4:1111268/140‑1 (MQ=14)
                                                                          cG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATca                                                                                                                                                        >  5:1190660/1‑77 (MQ=255)
                                                                          cG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACTAAATATTCATATATTAACGTAAAC‑‑A‑ATATCAACAGTAAAAAAA‑ATATCCCAATACATACATAGCAAAACAGTATAGAATACAAAGATACGAAACGAGAGGta                                                                                >  8:1433047/1‑147 (MQ=2)
                                                                              cggctctgcgcgtctaaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGt                                                                          <  3:212899/136‑1 (MQ=255)
                                                                              cggctaggagcgtctaaTCCTCCCGGATCCACGATCTCTTTGTTTACATGGCTTTAGTAGCGGTCTA‑‑A‑ATATCAGCAGTAAAATAA‑CTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGt                                                                          <  3:1187736/136‑1 (MQ=11)
                                                                                ggtatgcgtttataaTCCTCCGGGATTCAACCTCTCTTTCTTTATTTGGTTTTATTAGCTTTATA‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTc                                                                        <  6:1310471/138‑1 (MQ=14)
                                                                                gctCGGCGCTTCTAATCCTCCCGGATGCACCATCTCTTACTTTATATGGCTTTAGTAGCGGTATA‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTc                                                                        <  7:1240401/149‑1 (MQ=14)
                                                                                 gTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATACGTTGCTCAGCTGGATAGAGTACGCGGCTAAGAACCGAACgggcccagggtcg                                                                       >  7:82131/1‑140 (MQ=17)
                                                                                  tCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAgaataagt                                                                                                                                          >  6:1797488/1‑82 (MQ=255)
                                                                                  aaggagcgtctaaTCCTCCCTGATGCAAGATCTATTATTTTATATGGCTTTAGTAGCGTTATA‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCg                                                                       <  2:277667/139‑1 (MQ=11)
                                                                                   cggcgcttCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑AAATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGaa                                                                     <  7:505981/145‑1 (MQ=255)
                                                                                   cGGAGGTTCGAATCCTCCCGGATGCACCATATGGTACTTGATATGCATGTAGTAACGTTAAC‑‑A‑ATATCAGCAGTAAAAAAA‑ATTAGCCCATCCATAAGAAACACATCTTTATACAGAACACGGCAACGAAACGACAGCTCGGAGGTTAGa                                                                      >  6:355173/1‑150 (MQ=255)
                                                                                          gcgaatcctagatgATGCACCATCTCTTACTTGATCGGCCTCTAGGACGGGTATG‑‑A‑ATATCAGAAGTTAGCTAA‑TCGTCTCTATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTccc                                                              <  3:868768/138‑1 (MQ=2)
                                                                                           cGAATCCTCCCGGATGCACCATCTATTACTTGATATGTCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTccc                                                              <  7:403507/150‑1 (MQ=255)
                                                                                            gatatctccCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCgg                                                            <  1:1491373/146‑1 (MQ=255)
                                                                                            gAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCCTCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCgg                                                            >  3:1672892/1‑151 (MQ=255)
                                                                                             aagcctcgCGGAGGCACCATCTCTTACTTGATATGGCTATAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAACTAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCgg                                                            <  5:768769/147‑1 (MQ=255)
                                                                                             aaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTccc                                                              <  1:317961/148‑1 (MQ=40)
                                                                                             aaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGCTATA‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCgg                                                            <  5:44711/150‑1 (MQ=255)
                                                                                               tcctccCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTag                                                                                                                                                 >  5:647836/1‑63 (MQ=17)
                                                                                                  tccCGGATGCACCATCTATTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTACCGATGGATACGTAGCTCAGCTGGATAGAGTACTAGGCAACGAACCCAGCGGTCGGAGGTTGGAATCCTCCCGGATGccc                                                      >  8:1207793/1‑149 (MQ=14)
                                                                                                   cccGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCAcc                                                     >  4:309843/1‑151 (MQ=39)
                                                                                                     cGGATGCACCATCTCATACTTTATATGGCTATAGTAGCGATATC‑‑A‑AAATCAGCAGTAAACTAA‑CTTTCCCGATGCATCCGTAGCTCAGCTGGATAGACTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCat                                                   <  7:439408/151‑1 (MQ=11)
                                                                                                      ggATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCAGAGCGGGTTAGCGTAATCGGCTACCAACCGACCGGTCCGAGGTTCCAATCCACCCGGATGAAcgaa                                                   >  5:1847012/1‑147 (MQ=14)
                                                                                                                 ctctTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAt                                       >  8:171315/1‑151 (MQ=255)
                                                                                                                     tACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCCATGCATGCGTAGCACAGGGGGATAGCCTACGGGGGTAAGACCCGACCGGGCGGAGCTTCGGGTCGTCCCGGGTGCTCCATATTCTCAGAAATCtgc                                   >  7:70457/1‑149 (MQ=9)
                                                                                                                        ttGATACGGCTTTAGTAGCGGTATCAAA‑AAATCTGCAGTAAAGTAA‑TTATCCCGATGAATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCTGTCGGAGGATCGAATACACCCGGAAGCAACATATtctaataaataaac                                   >  6:1708726/1‑139 (MQ=14)
                                                                                                                        ttGATACGGCTTTAGTAGCGGTATCAAA‑AAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTcc                                  >  5:1662677/1‑150 (MQ=11)
                                                                                                                        ttGATACGGCTTTAGTAGCGGTATCAAA‑AAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCAGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGAAGCACCATATACTACgttatattg                                   >  8:1659142/1‑142 (MQ=17)
                                                                                                                         tGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAACTTCAGc                                >  5:1804617/1‑150 (MQ=255)
                                                                                                                         tGATACGGCTTTAGTAGCGGTATCAAA‑AAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTc                                   >  1:981319/1‑149 (MQ=11)
                                                                                                                          gATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCCCCATATTCTCCGAAATCTTCAGCa                               >  1:1319935/1‑150 (MQ=255)
                                                                                                                          gATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCa                               >  1:107396/1‑150 (MQ=255)
                                                                                                                          ccccgcccgcgccgagCGCTAAC‑‑A‑ATATTTGCAGTAAAATAA‑ATTTCCCGATGCCGCCTTATCGGTCGTGCATAGAGTTCACCGCTCCGATCCTAGCTTGTAGCGCGTCTAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCaa                              <  1:385078/137‑1 (MQ=255)
                                                                                                                             atcgCTTTACTAGGTGTATC‑‑A‑AAATAATCATTAAACTAA‑ATTACTCGATGAATCTCTAGCTATGATGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATg                            <  6:84907/147‑1 (MQ=14)
                                                                                                                             acggCTTTAGTAGCGGTATCAAA‑AAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACTAAATATTGATCAAt                             >  5:1574476/3‑151 (MQ=14)
                                                                                                                               ggCTTTAGTAGCGGTATCAAA‑AAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGCACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATACTCCGAAATCTTGAGAAATg                            >  6:303236/1‑150 (MQ=11)
                                                                                                                                  tttAGTAGCGGTATCAAA‑AAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAgg                        >  3:1560491/1‑151 (MQ=11)
                                                                                                                                   ttAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACTTCAGCAATGaaagat                      >  4:1159021/1‑146 (MQ=255)
                                                                                                                                    tttgaggtttata‑‑A‑ATATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTAt                     <  8:176649/141‑1 (MQ=11)
                                                                                                                                    ttataggtatata‑‑A‑ATATCTGCAGTAAATTAA‑TTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTAt                     <  2:718879/141‑1 (MQ=11)
                                                                                                                                      gtagCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAACTTCAGCAATGAAGGTATCg                   >  7:136732/1‑150 (MQ=255)
                                                                                                                                        aggtatata‑‑A‑ATATCAGCAGTAAATTAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGaa                 <  6:58872/145‑1 (MQ=255)
                                                                                                                                        agCGGTATCAAA‑AAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTCCTACATCTTCAGGTATGatcggatgg                   >  1:164097/1‑142 (MQ=14)
                                                                                                                                        agCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACTTCAGCAATGAAGGTaagat                  >  6:1467135/1‑145 (MQ=255)
                                                                                                                                        agCGGTATA‑‑A‑AATTCAGCCGTTAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCAATGAAGGTATCGaa                 <  7:925152/150‑1 (MQ=11)
                                                                                                                                          cGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTACCGATGCATCATTGGATCAGCTTGATATAGTACTAGGCTAATAACCTAGCGGTAGGAGGTTCGAATCCGCCCGGATTAAAAAAATTATCCGAAATGTTCAGCAATGAAGGGAtgggag                >  8:429960/1‑144 (MQ=9)
                                                                                                                                            ggtatcaaaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACGAAATATTCAGCTATGATCGTTTCGaat                >  3:629005/12‑149 (MQ=11)
                                                                                                                                            gctatcaaaaaatCTGCAGTAAATTAA‑CTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAaga               <  4:637899/140‑1 (MQ=14)
                                                                                                                                            gTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGt            >  5:1018433/1‑151 (MQ=255)
                                                                                                                                             gtatcaaaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAAATATTCATCAATGAACGTAACGaat                >  4:1242411/11‑148 (MQ=1)
                                                                                                                                              tatcaaaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGATATCTTCAGCAATGAAGGTATCGAAGAgg             >  3:1345129/10‑151 (MQ=14)
                                                                                                                                               tC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACTTCAGCAATGAAGGTATCGAAGAGATAGc         >  5:123384/1‑151 (MQ=16)
                                                                                                                                                ataa‑aTATCTGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTa           <  3:1061075/147‑1 (MQ=255)
                                                                                                                                                   aaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAAATATTCAGCAATGaa                          >  2:215443/5‑133 (MQ=11)
                                                                                                                                                   aaaaatCTGCAGTAAACTAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCg        <  1:594676/147‑1 (MQ=255)
                                                                                                                                                   a‑aTATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCAATGAAGGTATCGAAGAGATAGCgg       >  4:1528810/1‑151 (MQ=9)
                                                                                                                                                     atatCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGCCGGAGCGAAGCCTACAGCAGGCCCGAAACTCGCCACAATCTGCAGCAAGGAAGGTAGCGAAGAGGTAGc         >  3:299495/1‑148 (MQ=9)
                                                                                                                                                     aaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTGATCAATGAAGGTATGGAAGAGGTAGAgg       >  2:445848/3‑150 (MQ=14)
                                                                                                                                                      tatCAGCAGTGAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACTTCAGCa                               >  5:1404932/1‑125 (MQ=255)
                                                                                                                                                      tatCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCa                               >  2:1115837/1‑125 (MQ=18)
                                                                                                                                                      aatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCTAAATATTCAGCAATGAACGTATCGAagatcaagagga      >  4:1659765/2‑141 (MQ=11)
                                                                                                                                                      aatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATACGAAATATTCATAAATTAACGTATAGaatatcaacagta      >  2:647895/2‑139 (MQ=1)
                                                                                                                                                        tCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACTTCAGCAATGAAGGTATCGAAGAGATAGCgagat    >  1:619420/1‑146 (MQ=255)
                                                                                                                                                        tCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAtt   >  8:1345982/1‑151 (MQ=255)
                                                                                                                                                        tCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAtt   >  4:888009/1‑151 (MQ=255)
                                                                                                                                                         cagcagTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCgg                                                                              <  2:850264/75‑1 (MQ=40)
                                                                                                                                                          agcagTAAACTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTa                      <  1:177217/130‑1 (MQ=255)
                                                                                                                                                          |                                                                                                                                                      
CAAAAAATCTGCAGTAAAGTAAGTTTCCCGAT‑GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CG‑AGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑A‑ATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTA  >  DH1/1065457‑1065754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑