Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2970AG26.9% 372.4 / 146.2 203intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (116/25);  new base G (48/4);  total (166/37)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.82e-02

Only 100 of 211 total aligned reads displayed.

CCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGC  >  DH1/936160‑936447
                                                                                                                                          |                                                                                                                                                      
ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTAATGCTTTACTTCATCAAATTTTAATTGGTTGCgggg                                                                                                                                           >  8:107412/1‑151 (MQ=255)
          tttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGACTGCGAAACATCATCCAATTTGAAAAGGATGCCCGGCCCGGActtc                                                                                                                                 >  3:902245/1‑150 (MQ=11)
                         ttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTCCTTTACTTCATCAAATTTTAATTCGTTGAGGGGGCCGGATTTCAACCGACGACCTTCg                                                                                                                  >  8:723087/1‑151 (MQ=255)
                                                          gacCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGGACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGACGGATTTGAACCGACGACCTTCGGGTTATGAGACCGACGAGCTACCAGGCTGCTcc                                                                                 >  4:607764/1‑151 (MQ=255)
                                                                                       ccAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACt                                                    >  1:619196/1‑151 (MQ=255)
                                                                                            ctgctCCACCCCGCGTCACAGTACTGCTTTACTTCATCAAAATTTTATTGGTTCCGGCGGCAGTATTTGAACCGAACACCTTCGGGATATGAGACAGCCGAGATACATCGCTGCACCACCACGAGTCAGAGGAAGCGCACTATACTCCGc                                                >  6:902712/1‑150 (MQ=255)
                                                                                             tgctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTc                                              >  4:49475/1‑151 (MQ=255)
                                                                                              gctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGACCCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcg                                             >  2:476906/1‑151 (MQ=255)
                                                                                                tcaaCCCCGCCTCACCGTACTCCTTTACTTCATCAAATTTTAATTGGTTGCGGGCGCCGGATTTGAACCGACGACCTTCGGATTATGAGCCCGACGACCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGAGCACTATACTCCTCTcgcg                                           >  8:625039/4‑151 (MQ=17)
                                                                                                 ccACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGt                                          >  1:1362050/1‑151 (MQ=255)
                                                                                                    ccccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtttt                                       >  3:538790/1‑151 (MQ=255)
                                                                                                      ccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACTACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATAATCCGCTCGCGTTTTc                                      >  6:293579/1‑150 (MQ=255)
                                                                                                         cgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGAGGCGCACTATACTACGCTCGGTTTTTCAttc                                  >  6:1587786/1‑149 (MQ=11)
                                                                                                         cgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCCGGGGCCGGATTTGAACCGACGACCTTCGGGTTATTAGCCCGACGAGCTAACAGGCTCCTCCACACCACGTCCCAGGATTCGGAGTAAACACCCCTcgacaagtcattc                                  >  2:1567654/1‑140 (MQ=14)
                                                                                                         cgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                                  >  5:292587/1‑151 (MQ=255)
                                                                                                         cgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                                  >  8:1390500/1‑151 (MQ=255)
                                                                                                          gTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                                  >  4:1046280/1‑150 (MQ=255)
                                                                                                           tCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                                 >  2:401489/1‑150 (MQ=255)
                                                                                                            cACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGTATGCGAACTATACTCCGATAGATTTttcattcacc                               >  7:376905/1‑149 (MQ=11)
                                                                                                            cACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc                               >  7:819457/1‑151 (MQ=255)
                                                                                                             aCCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGATGCCCACTATACTCCGCACGGGATTTCATGCAAcc                              >  3:1129402/1‑151 (MQ=11)
                                                                                                             aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                              >  8:1283944/1‑151 (MQ=255)
                                                                                                               cGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGGACTATACTCCGCTATGGTTTTCATGCAACCtt                            >  6:1381485/1‑151 (MQ=14)
                                                                                                               cGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCCCAATATACTACGATAGCGTTTTCAt                                    >  4:920821/1‑143 (MQ=11)
                                                                                                               cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                            >  4:1725260/1‑151 (MQ=255)
                                                                                                               cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                            >  6:1005382/1‑151 (MQ=255)
                                                                                                                gTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGTCGCAATATACTCCGCTATCTTTTTGATGGAAccgtg                           >  3:1376707/1‑148 (MQ=11)
                                                                                                                gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                           >  2:1215815/1‑151 (MQ=255)
                                                                                                                gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                           >  3:1773194/1‑151 (MQ=255)
                                                                                                                gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                           >  3:355657/1‑151 (MQ=255)
                                                                                                                 tACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGCCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGCAGTATACTCCGCTCGCTTTTTCCTggaccggtgt                          >  3:56711/1‑142 (MQ=11)
                                                                                                                  aCTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGGGCACTATACTCCGCTCGATTTTTCATTCAAGCtttgt                         >  7:1269316/1‑149 (MQ=14)
                                                                                                                  aCTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt                         >  5:876469/1‑151 (MQ=255)
                                                                                                                   ctgcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGCGATGTATACTACGATCTGTTTTTCGTGCAGCGtgtttt                        >  1:468438/6‑151 (MQ=11)
                                                                                                                   cTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                        >  3:1513886/1‑151 (MQ=255)
                                                                                                                   cTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                        >  7:1228767/1‑151 (MQ=255)
                                                                                                                    tgcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGTTTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATTCGCACTATACTACGCTAGGGTATTCATGCAACCtgcgttt                       >  1:1274580/5‑145 (MQ=14)
                                                                                                                    tgcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGTATGCGGACTATACTACGCTCGGGTTTTCATGCAAGCtgttttt                       >  1:1121374/5‑151 (MQ=14)
                                                                                                                    tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                       >  6:142946/1‑151 (MQ=255)
                                                                                                                     gcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGATCGCGTTTTCATGCAACCtttttttt                      >  1:913123/4‑151 (MQ=255)
                                                                                                                     gcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACAAGGCAGCTCCACCCCGCGTCCGTGGATGCGAACTCTACAACCATCGATTTTTCATGcctcgttaattat                      >  2:941820/4‑139 (MQ=11)
                                                                                                                     gcttttACTTCTTAAATTTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                       <  8:613545/147‑1 (MQ=255)
                                                                                                                     gCTTTTCATCCTGAAAATTTCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                       <  3:208315/150‑1 (MQ=21)
                                                                                                                     gCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                      >  7:278034/1‑151 (MQ=255)
                                                                                                                     gCTTTAACTTATGAAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                       <  5:1438766/150‑1 (MQ=18)
                                                                                                                      cttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCCGACTATACTGCGCTCGCGTTTTCCTGCAAccggtgtttt                      >  1:442691/3‑142 (MQ=14)
                                                                                                                      cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                      >  1:1801198/1‑150 (MQ=255)
                                                                                                                        tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAc                    >  1:1726411/1‑151 (MQ=255)
                                                                                                                         ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcc                   >  6:1255994/1‑151 (MQ=255)
                                                                                                                         ttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTAGCGTTTTCCTGCAACCTTTTTTTTAacc                  >  5:1213899/1‑151 (MQ=18)
                                                                                                                         ttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGCACAATACTCCGCTCGCGTTTTCATGGAGCCTTTTTTTTAgcc                  >  2:192269/1‑151 (MQ=17)
                                                                                                                          tACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGCGCAGTATACTGCGATAGCTTTTTCATGCCAGCTTTATTTTAcccc                 >  2:1344968/1‑151 (MQ=14)
                                                                                                                            ttagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAGTATACTCCGATCGCTTTTTCATGCCACCTTGGTTTTAcccac                >  1:1556119/14‑149 (MQ=14)
                                                                                                                            cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTGTTCATGCAACCTTTTTTTTACCCCg                >  8:1729502/1‑151 (MQ=255)
                                                                                                                            cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCAGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg                >  2:800356/1‑151 (MQ=255)
                                                                                                                             ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAGCTTTTTTTTACCCCg                >  6:1820733/1‑150 (MQ=255)
                                                                                                                             ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg                >  7:357612/1‑150 (MQ=255)
                                                                                                                             tagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCCTTCAACCTTTTTTTTAACCCgc               >  3:1173450/13‑150 (MQ=17)
                                                                                                                             tagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg                <  7:1027668/138‑1 (MQ=255)
                                                                                                                             tagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGATCGCGtttt                                       >  1:1333024/13‑127 (MQ=14)
                                                                                                                             tagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAATATACTCCGATAGCTTTTTCATGCAACCTTTTTTTTAACCCg                >  4:1410378/13‑150 (MQ=17)
                                                                                                                              tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt              >  3:1817203/1‑151 (MQ=255)
                                                                                                                              agggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAGCCTTTTTTTTAACCCGAt              >  6:766607/12‑151 (MQ=18)
                                                                                                                              agggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGTAAGCGCACTATACTCCGAACGCTTTTTCATGCAACCTTTTTTATAacccca               >  4:640366/12‑148 (MQ=14)
                                                                                                                              agggtcatcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTcc                                                  <  1:1199711/104‑1 (MQ=255)
                                                                                                                               ggttgttatttCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt              <  5:1708616/142‑1 (MQ=255)
                                                                                                                                atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc             >  4:1499674/1‑150 (MQ=255)
                                                                                                                                atcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTATAACCCGATCAc           >  6:1736837/1‑151 (MQ=255)
                                                                                                                                atcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGGACTCTACTGCGCTGGCGTTTTCATGCAGCCGTTGTTTTAGCCCGATccc           >  1:1127469/1‑149 (MQ=14)
                                                                                                                                 gttttcaatAATTGGTTTCGGGGGCGGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGGTGCTCCACGCCGCGTCCGTGGATGCTCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTTCCCCGATCa            <  2:388290/142‑1 (MQ=21)
                                                                                                                                 gtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc           <  3:1088076/143‑1 (MQ=255)
                                                                                                                                 gtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTGTGTTTTACCCCGATCAc           >  2:542503/9‑151 (MQ=255)
                                                                                                                                  tgttcattAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTa                     <  4:727291/134‑1 (MQ=255)
                                                                                                                                  tgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCACCCTTTTTTTTa                     >  3:726212/8‑140 (MQ=255)
                                                                                                                                  tgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg                <  5:1491862/138‑1 (MQ=255)
                                                                                                                                   aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         >  4:1565255/1‑151 (MQ=255)
                                                                                                                                   aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         >  6:686771/1‑151 (MQ=255)
                                                                                                                                   aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCAGATCACgg         >  1:1678078/1‑151 (MQ=255)
                                                                                                                                    ttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         <  6:1467946/145‑1 (MQ=255)
                                                                                                                                    ttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         <  1:33338/145‑1 (MQ=255)
                                                                                                                                    ttaaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         <  2:808849/145‑1 (MQ=255)
                                                                                                                                    aaTTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGa        <  6:542397/151‑1 (MQ=255)
                                                                                                                                    aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         >  8:338852/1‑150 (MQ=255)
                                                                                                                                    aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         >  5:71044/1‑150 (MQ=255)
                                                                                                                                     tcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACta                                                        <  7:617294/98‑1 (MQ=255)
                                                                                                                                     tcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgcg                                           <  8:235929/111‑1 (MQ=255)
                                                                                                                                     tcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGa        <  4:440664/146‑1 (MQ=255)
                                                                                                                                      caatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg                                   >  5:1005406/4‑122 (MQ=255)
                                                                                                                                       tttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAttt     >  6:1706981/1‑151 (MQ=255)
                                                                                                                                       tttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAttt     >  4:689366/1‑151 (MQ=255)
                                                                                                                                       tttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAttt     >  4:81854/1‑151 (MQ=255)
                                                                                                                                       tttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAttt     >  5:1828320/1‑151 (MQ=255)
                                                                                                                                       aatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACAGGATGCGCAGTATACTAGGCTCGCTTATTCATGCAAGCTGTTTTTTAGCCCGCTCACGGAttt     >  7:532889/3‑151 (MQ=17)
                                                                                                                                        ttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGttt                                        <  1:1452822/115‑1 (MQ=255)
                                                                                                                                        atAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTg    <  8:1545569/150‑1 (MQ=255)
                                                                                                                                        atAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGAACTATACTCCGCtatatttt                                        >  2:1455765/2‑108 (MQ=255)
                                                                                                                                         tAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACGTTTTTTTTACCCAGATCACGGATTTgg   >  7:1043431/1‑151 (MQ=255)
                                                                                                                                         tAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTgg   >  7:1609365/1‑151 (MQ=255)
                                                                                                                                          aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCTCTATACTCCGCTCGAGTTTTCTTGCAACCTTTTTTTTACCCCGATCCCGGATTTggg  >  7:920544/1‑150 (MQ=255)
                                                                                                                                          aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGc  >  5:1550889/1‑151 (MQ=255)
                                                                                                                                          |                                                                                                                                                      
CCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGC  >  DH1/936160‑936447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: