Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1810,3340GA72.7% 927.0 / inf 541W88* (TGG→TAG) gapAD‑erythrose‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (73/74);  new base A (220/171);  total (294/247)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 544 total aligned reads displayed.

GGCGGATGCTGCGGGCATGGCGCATTTGTTGAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGATCTCGACGCGACCGTTGTTTACGGCGT  >  DH1/810185‑810484
                                                                                                                                                     |                                                                                                                                                      
 gCGGATGCTGCGGGCATGGCGCATTTGTTGAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGc                                                                                                                                                      >  4:180547/1‑151 (MQ=255)
  cGGATGCTGCGGGCATGGCGCATTTGTTGAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCg                                                                                                                                                     <  1:1118739/151‑1 (MQ=255)
   ggATGCTGCGGGCATGGCGCATTTGTTGATATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGt                                                                                                                                                    <  5:1828418/151‑1 (MQ=255)
   ggATGCTGCGGGCATGGCGCATTTGTTGAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGt                                                                                                                                                    >  6:262116/1‑151 (MQ=255)
   ggATGCTGCGGGCATGGCGCATTTGTTGAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGt                                                                                                                                                    >  1:414746/1‑151 (MQ=255)
    gaggctgcGGGCAGGGCGCATTTGTTGAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTg                                                                                                                                                   <  6:406805/148‑1 (MQ=255)
      tgctgcGGGCATGGCGCATTTGTTGAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTACACTGCAATCGCTCCCATAGCGTGa                                                                                                                                                  >  1:1029592/1‑150 (MQ=255)
       gctgcGGGCATGGCGCATTTGTTGAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAAc                                                                                                                                                >  7:805450/1‑151 (MQ=255)
          gcGGGCATGGCGCATTTGTTGAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACtt                                                                                                                                              <  6:1613192/150‑1 (MQ=255)
            gggCATGGCGCATTTGTTGAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGc                                                                                                                                           <  2:1564131/151‑1 (MQ=255)
             ggCATGGCGCATTTGTTGAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCg                                                                                                                                          <  1:1266195/151‑1 (MQ=255)
             ggCATGGCGCATTTGTTGAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCg                                                                                                                                          <  5:1615249/151‑1 (MQ=255)
              gCATGGCGCATTTGTTGAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGCCAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGATCGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGt                                                                                                                                         >  8:1481217/1‑151 (MQ=255)
                   gcgcATTTGTTGAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATg                                                                                                                                    >  2:1325760/1‑151 (MQ=255)
                    cgcATTTGTTGAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGt                                                                                                                                   >  4:721335/1‑151 (MQ=255)
                            ttgAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCg                                                                                                                           >  1:1824051/1‑151 (MQ=255)
                             tgAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCg                                                                                                                           >  1:479025/1‑150 (MQ=255)
                               aaaTATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACt                                                                                                                        <  4:1475213/151‑1 (MQ=255)
                                   atGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCAc                                                                                                                    <  6:536585/151‑1 (MQ=255)
                                     gACACCCGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCACTCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCGCGCCTGCCCCg                                                                                                                  >  2:22672/1‑151 (MQ=255)
                                     gACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCAcc                                                                                                                   <  6:1119893/150‑1 (MQ=255)
                                      acacCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCgg                                                                                                                 <  8:559701/151‑1 (MQ=255)
                                      acacCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCgg                                                                                                                 >  7:1311957/1‑151 (MQ=255)
                                        acCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCg                                                                                                               >  5:1088846/1‑151 (MQ=255)
                                         ccAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGt                                                                                                              <  5:1230731/151‑1 (MQ=255)
                                          cAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGt                                                                                                              >  6:1661653/1‑150 (MQ=255)
                                          cAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGt                                                                                                              >  6:1014270/1‑150 (MQ=255)
                                          cAGCCATGGCCGATTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGta                                                                                                             <  6:898076/151‑1 (MQ=255)
                                             ccATGGCCGTTTTGCATGGGAAGTACGACAGGACCGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTatat                                                                                                          >  2:959861/1‑151 (MQ=255)
                                              cATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATg                                                                                                         <  3:93724/151‑1 (MQ=255)
                                               aTGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATgg                                                                                                        >  3:228949/1‑151 (MQ=255)
                                               aTGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATgg                                                                                                        <  8:187180/151‑1 (MQ=255)
                                                    cGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGCCGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTccc                                                                                                   >  5:716942/1‑151 (MQ=255)
                                                    cGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTccc                                                                                                   >  7:679243/1‑151 (MQ=255)
                                                     gTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCcg                                                                                                  <  6:1346274/151‑1 (MQ=255)
                                                     gTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTcccc                                                                                                  >  7:612161/1‑150 (MQ=255)
                                                        ttGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCcgcg                                                                                                >  3:1519369/1‑150 (MQ=255)
                                                            aTGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCAt                                                                                           >  5:283965/1‑151 (MQ=255)
                                                             tGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATg                                                                                          >  7:1442937/1‑151 (MQ=255)
                                                              gggAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATg                                                                                          <  8:36306/150‑1 (MQ=255)
                                                              gggAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATgg                                                                                         >  5:599069/1‑151 (MQ=255)
                                                               ggAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGc                                                                                        <  4:354420/151‑1 (MQ=255)
                                                                gAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCg                                                                                       <  7:1740566/151‑1 (MQ=255)
                                                                 aaGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCg                                                                                       >  7:494414/1‑150 (MQ=255)
                                                                 aaGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAATTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCg                                                                                       >  6:686660/1‑150 (MQ=255)
                                                                 aaGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCg                                                                                       >  4:972817/1‑150 (MQ=255)
                                                                   gTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAg                                                                                    <  4:779770/151‑1 (MQ=255)
                                                                    tACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAgc                                                                                   >  4:406600/1‑151 (MQ=255)
                                                                        aCAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCat                                                                               >  1:150675/1‑151 (MQ=255)
                                                                         cAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCat                                                                               >  1:982112/1‑150 (MQ=255)
                                                                            gAAGGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATAtt                                                                            <  4:257103/150‑1 (MQ=255)
                                                                            gAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTg                                                                           >  8:1329617/1‑151 (MQ=255)
                                                                             aaCGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTgc                                                                          >  7:1155238/1‑151 (MQ=255)
                                                                              aCGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTgcc                                                                         >  4:1172079/1‑151 (MQ=255)
                                                                              aCGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTgcc                                                                         >  6:1731892/1‑151 (MQ=255)
                                                                                gcgATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTgccgc                                                                       <  4:528099/151‑1 (MQ=255)
                                                                                  gATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGccgccg                                                                     >  2:705518/1‑151 (MQ=255)
                                                                                      aaCTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGc                                                                 <  5:417622/151‑1 (MQ=255)
                                                                                       aCTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGcc                                                                >  6:1254342/1‑151 (MQ=255)
                                                                                          ttttGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCa                                                               <  7:223453/149‑1 (MQ=255)
                                                                                           tttGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCaaa                                                             <  5:842211/150‑1 (MQ=255)
                                                                                              gttgGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGCCTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCaaaaa                                                           >  8:1067252/1‑149 (MQ=255)
                                                                                                  gTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGct                                                     >  1:593844/1‑151 (MQ=255)
                                                                                                     atgaCGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCtt                                                  >  4:1690278/1‑151 (MQ=255)
                                                                                                       gaCGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCtttt                                                <  8:142547/151‑1 (MQ=255)
                                                                                                          gCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTcac                                             >  3:1223274/1‑151 (MQ=255)
                                                                                                          gCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTcac                                             >  3:1110508/1‑151 (MQ=255)
                                                                                                          gCCATCCCCGTATTGCATGAACGTTAACTGCAATCGCTCCCCTAGCGTGCACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGCGGCCAAAAAAGTGCTCTTTTcac                                             >  1:714141/1‑151 (MQ=255)
                                                                                                            cATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCGCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACAt                                           <  2:319130/151‑1 (MQ=255)
                                                                                                            cATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACAt                                           <  3:417859/151‑1 (MQ=255)
                                                                                                             aTCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATc                                          >  8:127401/1‑151 (MQ=255)
                                                                                                             aTCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAACTGCTCTTTTCACATc                                          >  8:878767/1‑151 (MQ=255)
                                                                                                              tCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAGGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATcc                                         >  2:1777311/1‑151 (MQ=255)
                                                                                                               ccGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCt                                        <  4:1420624/151‑1 (MQ=255)
                                                                                                                 gcgTATTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTgg                                      >  1:294097/1‑151 (MQ=255)
                                                                                                                     aTTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAg                                   <  2:1116270/150‑1 (MQ=255)
                                                                                                                     aTTGCATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAg                                   <  7:330824/150‑1 (MQ=255)
                                                                                                                        gCATGAACGTTCACTGCGATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTaa                                <  4:598474/150‑1 (MQ=255)
                                                                                                                         cATGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACg                              <  1:1665715/151‑1 (MQ=255)
                                                                                                                          aTGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACg                              >  5:1462313/1‑150 (MQ=255)
                                                                                                                          aTGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGa                             <  5:137430/151‑1 (MQ=255)
                                                                                                                           tGAACGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGAt                            >  3:1362746/1‑151 (MQ=255)
                                                                                                                             aaCGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGAtct                          >  2:1419069/1‑151 (MQ=255)
                                                                                                                             aaCGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGAtct                          >  8:707481/1‑151 (MQ=255)
                                                                                                                             aaCGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGAtct                          <  6:1519382/151‑1 (MQ=255)
                                                                                                                              aCGTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGAtctc                         <  6:18727/151‑1 (MQ=255)
                                                                                                                                gTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGATCTCGa                       <  1:866464/151‑1 (MQ=255)
                                                                                                                                gTTCACTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGATCTCg                        >  4:1075941/1‑150 (MQ=255)
                                                                                                                                     cTGCAATCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGATCCCGCGAGCATGGCGAAGCGCATATTCCCGCCGGGGACAAAAACGTGCACTTTTCACATCCTGGCAGTAAATATATCGAcacg                   >  7:967652/1‑150 (MQ=255)
                                                                                                                                       gCAATCGCTCCCCTAGCGTGAACTTGGGGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTGACATCCTGGCAGTAACGATCTCGAGGCGa                  <  4:1255939/149‑1 (MQ=255)
                                                                                                                                         aaTCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGATCTCGACGCGACCgt              >  2:452990/1‑151 (MQ=255)
                                                                                                                                          aTCGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGATCTCGACGCGACCgtt             <  3:720336/151‑1 (MQ=255)
                                                                                                                                            cGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGCCTGCACCGGCGTATATGGATCCAGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTCCTCTTTTCACATCATCGCAGTAACGATCTCGACGCGACCgttct           >  8:163874/1‑149 (MQ=255)
                                                                                                                                            cGCTCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGATCTCGACGCGACCgttgt           >  1:1412099/1‑151 (MQ=255)
                                                                                                                                             gCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCAAATCCTGGCAGTAACGATCTCGACGCGACCgttgtt          >  3:1630966/1‑151 (MQ=255)
                                                                                                                                               tCCCCTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGATCTCGACGCGACCGTTGttt         >  7:1547511/1‑150 (MQ=255)
                                                                                                                                                ccccTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGATCTCGACGCGACCGTTGTTTa        <  8:460567/150‑1 (MQ=255)
                                                                                                                                                ccccTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGATCTCGACGCGACCGTTGTTTAc       <  3:34773/151‑1 (MQ=255)
                                                                                                                                                 cccTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGATCTCGACGCGACCGTTGTTTACg      >  6:145997/1‑151 (MQ=255)
                                                                                                                                                 cccTAGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGATCTCGACGCGACCGTTGTTTACg      >  7:1660709/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GGCGGATGCTGCGGGCATGGCGCATTTGTTGAAATATGACACCAGCCATGGCCGTTTTGCATGGGAAGTACGACAGGAACGCGATCAACTTTTTGTTGGTGATGACGCCATCCGCGTATTGCATGAACGTTCACTGCAATCGCTCCCCTGGCGTGAACTTGGCGTTGATGTAGTCCTCGACTGCACCGGCGTATATGGCTCCCGCGAGCATGGCGAAGCGCATATTGCCGCCGGGGCCAAAAAAGTGCTCTTTTCACATCCTGGCAGTAACGATCTCGACGCGACCGTTGTTTACGGCGT  >  DH1/810185‑810484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑