Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,7700CA26.0% 257.6 / 24.6 210intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base C (107/21);  new base A (45/0);  total (186/24)
Fisher's exact test for biased strand distribution p-value = 2.33e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTC  >  DH1/613633‑613919
                                                                                                                                         |                                                                                                                                                       
       ggCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGAAGAAAGACAGTCCACGACACAAAACCAAAAAAAAAGAGTCTAAAACGTCAGTTTCACCTGTTAAACATaaaaaa                                                                                                                                      >  6:550894/1‑149 (MQ=11)
         cTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAACCCg                                                                                                                                   >  3:777219/1‑151 (MQ=25)
          ttATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGCCAGAAAGATCAATGACTGTCCACCACACTATACCCAAAAGAAACCGGCTTATCGCGCACTATGACCTGTTGTACGTAATAACCCgt                                                                                                                                  >  7:339784/1‑150 (MQ=11)
            aTCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGATTTTTGAGGGGCAGAAAGATGAAACACTTTCCACGACAAAAAAGCCAAAAGAAAGCGAAATATCGGTAAGTTTAAAGTGTTATAAGTAATAAAACG‑Ct                                                                                                                                >  2:1526823/1‑150 (MQ=2)
            aTCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTACGGCGGGTTTATGCTTTTGGAGGGGCAGAAAGATGAATGACTTTCCACGAAACTATACCCAAAAGAAAGCGGATTATCGTTAAGTTTAAACTGATTTACGTAAAAAACCgat                                                                                                                                 >  6:1092504/1‑148 (MQ=11)
              cGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGTGGCAGAAAGATGAATGACTTTCGACGACACTATAGACAAAAGAAAGCGGCTTATCGGTCAGTTTAACATGATTTACGTAAAAACCCG‑CTCcg                                                                                                                             >  1:615054/1‑151 (MQ=11)
              cGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATAAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTATAACCTGATTTACGTAAAAAACCgatac                                                                                                                               >  6:1317116/1‑146 (MQ=11)
               ggTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTGTACGTAAAAAACCG‑ATGcgg                                                                                                                            >  4:319664/1‑151 (MQ=255)
                 tCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCG‑CTTcggcg                                                                                                                          >  3:1525464/1‑151 (MQ=25)
                  ccggagccccTGGTTTTCGTAAGAGCGCGCTTCGGCTGTTTTGTGTTGTTGAGGGCGCGCCACCTTGATGGACTTGCCACGACACTCTACCCAACAGAACGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTcggcgg                                                                                                                         <  1:234843/143‑1 (MQ=2)
                  cAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTTATTTACGTAATAACCCG‑ATAcggcgg                                                                                                                         >  6:393150/1‑151 (MQ=11)
                   aGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACAGTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAATAAACCG‑ATTCGGCggg                                                                                                                        >  5:1662483/1‑151 (MQ=11)
                    gTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCG‑CTTCGGCGGGt                                                                                                                       >  5:965221/1‑151 (MQ=25)
                     tttCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAAACCG‑TTGCGGCGGGtt                                                                                                                      >  5:880158/1‑151 (MQ=255)
                       gcACCGGGCTCACGAAAAAAGCCGCGGGGGGGTGTTTTTTCCTTTGGTGGGCCCTACCTATCAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCG‑TTCCGGCGGGtttc                                                                                                                    <  1:1820952/150‑2 (MQ=9)
                        ccccggcttcaCGTAAAACCCCGCTTCGGCGGTTTTTTTTTTTTTTATGTTAAGACTCATTAATTACTGTCCACTACTCTATACCCAATAGAAAGCGTCTTATCGGTCAGTTTCTCCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGttttt                                                                                                                   <  8:1650396/141‑1 (MQ=2)
                        cACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCG‑ATAAGGCGGGttta                                                                                                                    >  2:1274156/1‑149 (MQ=11)
                         aCCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCG‑GTTCGGCGGGtttttg                                                                                                                  >  1:467663/1‑150 (MQ=25)
                          ccTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCG‑CTGCGGCGGGtttgtgc                                                                                                                 >  3:1704762/1‑147 (MQ=255)
                          ccTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTACGGCGGGTTTGTa                                                                                                                  >  6:770424/1‑150 (MQ=25)
                            tGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTGCGGCGGGTTTTTGCtt                                                                                                               >  5:182279/1‑151 (MQ=9)
                             ggTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAATAAACCG‑GTCCGGCGGGtttgaccttg                                                                                                              >  5:531085/1‑144 (MQ=11)
                              gTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑AAACGGAGGGTTTGAACtttt                                                                                                             >  2:116788/1‑151 (MQ=11)
                              gTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAACAG‑ATACGGCGGGtttaaaatttt                                                                                                             >  4:1278845/1‑143 (MQ=11)
                               tttACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACttttt                                                                                                            >  2:787192/1‑150 (MQ=18)
                               tttACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCG‑ATACGGCGGGtttaatattttt                                                                                                            >  4:1790122/1‑142 (MQ=11)
                                ttACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTgg                                                                                                           >  5:445943/1‑151 (MQ=25)
                                 tACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAACCCG‑GTTCGGCGGGTTTGTGCTTGTggg                                                                                                          >  3:1281689/1‑150 (MQ=11)
                                      aaaaaCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTGCGGCGGGTTTTTACTTTTGGAGTGGc                                                                                                     >  8:551924/1‑151 (MQ=16)
                                      aaaaaCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCG‑ATTCGGCGGGTTTATGCTTTTTGAgtgcc                                                                                                     >  2:274294/1‑147 (MQ=11)
                                       aaaaCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAATAACCCG‑ATGCGGCGGGTTTCAAATTTTGGAgtgcg                                                                                                     >  7:1457544/1‑146 (MQ=11)
                                          aCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCG‑ATTCGGCGGGTTTAAACTTTTTGAgtgcgata                                                                                                  >  4:284118/1‑143 (MQ=11)
                                           cccGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTCTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGCTCAGTTTCACCTGATTTACGTAAAAACCCG‑ATACGGCGGCTTTTAACTTTTTGAGGGCCAGaa                                                                                                 >  8:364949/1‑150 (MQ=11)
                                            ccGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCAAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCG‑ATTCGGCGGGTTTGTACTTGTGGAgtgcgagttat                                                                                               >  6:340367/1‑141 (MQ=11)
                                              gCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCG‑ATTCGGCGGGTTTAAACTTTTTGAgtgcgataaaga                                                                                              >  1:317790/1‑139 (MQ=11)
                                                ttCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTGCGGCGGGTTTTTCCTTGTTGAGGGGGAGTAAGatgt                                                                                           >  7:432326/1‑150 (MQ=11)
                                                  cggcggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCG‑ATTCGGCGGGTTTTAAATTTTTGAgtgcgataaagtaaat                                                                                          >  6:1527462/1‑135 (MQ=11)
                                                   ggcggcTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCCGTCAGTTTAACCTGATTTACGTAAAAAACCC‑ATACGGCGGGTTTAAAATATTGGAGTGCCATaaaaaaaaaac                                                                                        >  6:307327/1‑143 (MQ=11)
                                                    gcggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTTCTTTTGGAGGGGGAGAAAGaggaggcc                                                                                       >  3:704251/1‑147 (MQ=11)
                                                         tttttGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTCTTTTACGTAAAAAACCG‑ATACGGCGGCTTTCTCCTTTTGGAGGGCGAGATAGaagtaagggagt                                                                                   >  4:871724/1‑139 (MQ=11)
                                                              gCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCG‑GTGCGGCGGGTTTTTACTTTTTGAGTGGCAGAAATatgatagcctttcgacg                                                                              >  7:1340934/1‑137 (MQ=11)
                                                                   tGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑ATTCGGCGGGTTTGTCCTTTTGGAGGGGGAGAAAGAAGAAGCACA‑GTCGCCGACACt                                                                        >  8:1351687/1‑150 (MQ=11)
                                                                      aGGGGCAGAAAGATGAATGACTGTCCACGACACTATACAAAAAAAAAACCTCCTTATAGGTAAATATAAAAAAGATTACGTAAAAACACG‑CTTCGCCCCGTTTAAAAAAAAAAAAAGGCAGAAAGATGAATGACT‑GTCCACGACACTATAc                                                                    >  6:516087/1‑151 (MQ=2)
                                                                           cAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCG‑ATACGGCGGGTTTTTACTTTTTGAGGGCCAGAAAGAAAAAACACT‑TTCCACGACACtataacaaa                                                                >  8:780837/1‑145 (MQ=11)
                                                                            aGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTGCGGCGGGTTTGACATTTTGGAGTGGCAGAAAGAGCAAAGACT‑GGCCACGacacgctagacaac                                                               >  7:1384415/1‑140 (MQ=11)
                                                                            aGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCG‑ATACGGCGGGTTTGTACTTTTTGAGTGCCAGAAAGAAGAAACACA‑GTCCACGACACGAAACTCaaat                                                              >  8:1152268/1‑150 (MQ=11)
                                                                             gAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCG‑GTGCGGCGGGTTTGTGCTTTTTGAGTGCGAGATATAGGAATGACT‑GGCGCCGacacgctcggcaaatg                                                             >  1:1381071/1‑139 (MQ=11)
                                                                                  atgaatgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCG‑CTGCGGCGGGTTTGTGCTTTTTGAGTGGCAGTAAGATGAATCCCT‑TGCGACGACACTCTACACAaaagacatc                                                        >  3:56218/1‑147 (MQ=11)
                                                                                   taccgacgtggccACGACCCTTAGCCCAATACAACCCGGGTTATGGGTCTGTTCCCCCTTTTTTACGTAAAACCCCG‑GTGGGGGGTTTTTTTCCTTTTGGTGGGGCAAAAAGATAATGGCCG‑GTCCACGACACTATACCCAAAAGAAAGc                                                        <  7:813237/139‑1 (MQ=2)
                                                                                    gaatgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTCCTTTTGGAGGGGCAGAAAGATGACTGCCT‑GTCCCCGACACTATACCAAAAAGAAAGCgc                                                      >  7:1025478/1‑150 (MQ=255)
                                                                                          cTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGCCT‑GTCCACGACACTCTACCCAAAAGAAAGCGGCTTATc                                                >  1:393171/1‑151 (MQ=25)
                                                                                             tCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTAATTTTGGAGTGGCAGAAAGAAGTATGACT‑GTCCCCGACACAATCCCCAAAAGAAAGCGGCATAACGGt                                             >  5:34490/1‑151 (MQ=11)
                                                                                             tCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTATACTTTTGGAGGGCCAGAAAGAAGAAAGACT‑GTCCACGACACAAGACCAAAAAGCAAGCGGCTTATCgc                                              >  2:609214/1‑149 (MQ=11)
                                                                                                 cGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCG‑GTGCGGCGGGTTTTTAATTTTGGAGGGGCAGAAAGAGGTGGGCCT‑GTCGACGACAAGGTCGCAACAAGAATCCTGCTTATCGGTCAGt                                         >  7:758272/1‑151 (MQ=2)
                                                                                                  atcacTCTCCCCAAAAGAACCGTGCTTATCGTTTATTTTTAACTTATTTACTTAAAAACCAG‑CTTTTGCGTTTTTTTAATTTTGTTGGGGAACAATTATTAATTACT‑TTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGtt                                        <  2:1372707/149‑1 (MQ=2)
                                                                                                      cTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCG‑GTGCGGCGGGTTTTTGCTTTTTGAGGGGCAGAAATATCAGAGCCT‑TTCGCCGACACGATAGGCTAATGCCAGCGGCTGATCGGTAAGTTTTa                                     >  3:1141393/1‑150 (MQ=2)
                                                                                                      cTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑GTTCGGCGGGTTTTTACTTTTGGAGTGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTa                                     >  5:1268958/1‑150 (MQ=25)
                                                                                                      cTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTGCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCGACGACACTATCCACAAAAGAAAGCGCCTTATCGCTAATTTTTAc                                    >  3:669048/1‑151 (MQ=11)
                                                                                                      cTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTGCGGCGGGTTTTAACTTTTGGAGGGCCATAAAGATGAAAGACA‑GTCCACGACAAAAAAAGCAAAAGAAATCCGCTTATCGGTCAGTATTAc                                    >  3:946351/1‑151 (MQ=11)
                                                                                                       tataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGCCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTAc                                    >  8:466222/1‑150 (MQ=25)
                                                                                                        ataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGAGACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTaaat                                  >  2:162989/1‑148 (MQ=25)
                                                                                                        ataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGAAGAAAGCCT‑TTCCACGACACAATACCAAAAAGAAAACGGCATATCGCACAGtaataaa                                   >  8:488589/1‑144 (MQ=11)
                                                                                                          aCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATCCCCAAAAGAAAGCGCCTTATCGGTCAGTGTTACCTg                                 >  7:385099/1‑150 (MQ=255)
                                                                                                          aCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTCTACTTGTTGAGGGGCAGAAAGAGCTATCCCT‑GTCCCCGAGACGGACCCCAAAAGAATACGACTTCACGGTCATTGTTACCtct                                >  1:930982/1‑149 (MQ=2)
                                                                                                          aCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑ATTCGGCGGGTTTTTACTTTTGGAGTGGCAGAAATATCAAAGACT‑TTCCCCGACACTATAAAAAAAAGAAAGCGGCTTATCGGTAAGTCTTaaatgt                                >  4:993728/1‑146 (MQ=11)
                                                                                                           cccAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTAATTTTGGAGGGCCAGAAAGAGCAAACCCT‑GTCCACGACACTAGACGCAAAAGAAAGCGGATTATCGGTAAGTTTTACATGAt                               >  7:1595895/1‑151 (MQ=11)
                                                                                                           cccAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTTGAGGGGCAGAAAGAGGATACCCT‑TTCCTCGAGACTATACACAAATGAAAGCGGCTTATCGCTCAGtaattcctttt                               >  5:1168875/1‑141 (MQ=11)
                                                                                                              aaaaGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑ATTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAATAAGAATGACT‑GTCCACGACACTAAACGCAAAAGAAAGCGGCTTATCGGTCAGTGTTAACTTATGt                             >  4:1371927/1‑150 (MQ=11)
                                                                                                               accgaacccGGCTTTTCTTTCAGTATCACCTGATTTACGTAAAAACCCGCCTGCGGGCGTTTTTTTCTTTCGTGGGCGC‑TTCCGATCTTTGCCTCGACCA‑GACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTAc                           <  1:1026827/143‑1 (MQ=2)
                                                                                                               aaagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑GTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACA‑TTCCACGACACTATACCCAAAAGAAAGCGTATTATCGGTCAGTTTTACCTGATGTAc                           >  5:1403747/1‑151 (MQ=255)
                                                                                                               aaagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTAc                           >  7:898045/1‑151 (MQ=40)
                                                                                                                 agaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATCCCCAAAAGAAAGCGGCATATCGGTCAGTTTTACCTGATGTACGt                         >  8:1025940/1‑151 (MQ=255)
                                                                                                                  gaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGCTCAGTTTTAAATGATGTACGt                         >  6:608642/1‑150 (MQ=255)
                                                                                                                   acagCGCCTTATGGGTCCGGTGTGCCTGTTTTACGTACTTTGGCT‑CTTCCGCGGTTTTTTTCTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaa                       <  1:1297550/149‑1 (MQ=11)
                                                                                                                     agCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGCCT‑GTCGACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaat                      >  2:1441848/1‑150 (MQ=255)
                                                                                                                      gCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACTtaata                     >  6:1670205/1‑150 (MQ=255)
                                                                                                                       cGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAACGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaataa                    >  2:1385298/1‑150 (MQ=255)
                                                                                                                        ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAc                  >  8:634627/1‑151 (MQ=255)
                                                                                                                        ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGACAGTTTTACCTGATGTACGTAATAAAc                  >  4:43134/1‑151 (MQ=255)
                                                                                                                        ggCTTATCGGTCAGTTTAACCTGGTTTACGTAAAAACACG‑GATCAACAGCTTATTGCTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACACAAAATAAAACGGCTTATCGGTCAGTTTGACCTGATGTACGTAATAAAc                  >  6:689328/1‑151 (MQ=11)
                                                                                                                         gcataggggggcgTTTCCCTTGTTTTACTTAAAAACCCG‑CTGCGGGGGTTTTTTTTTTTTGGTGGGGCACAAAGATTAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAcc                 <  7:3074/139‑1 (MQ=14)
                                                                                                                         gCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAcc                 >  5:786812/1‑151 (MQ=255)
                                                                                                                         gCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTATGATTTTTGAGTGCCATAAAGAAAAAAGACA‑GTCCACGACAAAATACACAAAAGAAAGCGACTTATCGCACATTAAAACATGATGAACGTaaaaaaac                 >  4:1116044/1‑149 (MQ=2)
                                                                                                                          cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                >  1:1600180/1‑151 (MQ=255)
                                                                                                                           ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTTGTTTTGGAGGGGCAGAAATATGAATGACT‑GTCCACGACACTATACCCAaaagaaag                                                         <  4:708640/109‑1 (MQ=255)
                                                                                                                           ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt               >  4:1094041/1‑151 (MQ=255)
                                                                                                                           ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt               >  2:1106806/1‑151 (MQ=255)
                                                                                                                           ttATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt               >  4:1613745/1‑151 (MQ=255)
                                                                                                                           ttATCGGTCAGTTTAACCTGATTTACGTAAAAACAAG‑ATGCTCGTGGTTTTTAATATTGGTGCGGAAAAAAGATGAATGACT‑GTACACCACAATATAAAAAAAAGAAAGCGGCTTATCGGTCTGTTTTAGGTGATGTGCGtgatgggaggg               >  2:936950/1‑144 (MQ=2)
                                                                                                                            tATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGtt              >  7:1097332/1‑151 (MQ=255)
                                                                                                                              tCGGTCAGTTTCACATGTTTTACGTAAAAACCCG‑ATTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCAAGACACTATACCCAAAAGAAATCGGCTTATCGGTCAGTTTTAACTGATGTACTTAATAAACCGTTc             >  5:1593547/1‑150 (MQ=17)
                                                                                                                                  tCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcg        >  7:545426/1‑151 (MQ=255)
                                                                                                                                   cAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGTGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcgg       >  3:873496/1‑151 (MQ=255)
                                                                                                                                   cAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcgg       >  4:777377/1‑151 (MQ=255)
                                                                                                                                    aGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACATGATGTACGTAATAAACCGTTCCGTCggg      >  8:1405125/1‑151 (MQ=255)
                                                                                                                                      tttCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGtt    >  5:1634455/1‑151 (MQ=255)
                                                                                                                                       ttCACCTGTTTTACGTAAAAACCCG‑CTTGGGGGGGTTTTTTCTTTTGGAGGGGCATAAAGATTAATTACT‑TTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGtt    <  6:439942/150‑1 (MQ=17)
                                                                                                                                         cACCTGTTTTACGTAAAACCCCG‑CTTCGGCGGGTTTTTCCTTTTGGAGGGCCACAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc  <  6:880169/150‑1 (MQ=255)
                                                                                                                                         cACCTGTTTTACGTAAAAACCCG‑CTTCGGGGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCAGGCGGGTTTc  >  5:105600/1‑150 (MQ=255)
                                                                                                                                         cACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc  >  1:851808/1‑150 (MQ=255)
                                                                                                                                         |                                                                                                                                                       
AGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACT‑GTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTC  >  DH1/613633‑613919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑