Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 2,404,019 +G coding (239/861 nt) ydbC ← oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,404,0181.G96.8% 1271.0 / 26.4 340coding (240/861 nt)ydbCoxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (7/4);  new base G (170/158);  total (178/162)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 359 total aligned reads displayed.

CCCCATCATAACGCGCAGGTTAACCACATCCAGCACGTCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATT‑GTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACGGTTATAGCGACGTGGCGATCTCGTG  >  DH1/2403868‑2404163
                                                                                                                                                       |                                                                                                                                                 
 cccATCATAACGCGCAGGTTAACCACATCCAGCACGTCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATT‑g                                                                                                                                                  <  2:1622398/151‑1 (MQ=255)
 cccATCATAACGCGCAGGTTAACCACATCCAGCACGTCCACCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATT‑g                                                                                                                                                  <  6:686387/151‑1 (MQ=255)
   catcatCACGCGCAGGTTAACCACATCCAGCACGTCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATT‑ggt                                                                                                                                                <  6:1805341/151‑3 (MQ=255)
   catcatAACGCGCAGGTTAACCACATCCAGCACGTCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATT‑ggt                                                                                                                                                >  3:435856/1‑149 (MQ=255)
      catAACGCGCAGGTTAACCACATCCAGCACGTCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATT‑ggtc                                                                                                                                               >  7:1306549/1‑146 (MQ=255)
      catAACGCGCAGGTTAACCACATCCAGCACGTCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGAAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATT‑ggtc                                                                                                                                               >  7:745676/1‑146 (MQ=255)
       atAACGCGCAGGTTAACCACATCCAGCACGTCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACAAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATT‑Gttcagg                                                                                                                                            >  5:1091014/1‑146 (MQ=255)
             cgcAGGTTAACCACATCCAGCACGTCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGgtcgtc                                                                                                                                        >  8:587100/1‑150 (MQ=255)
             cgcAGGTTAACCACATCCAGCACGTCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCa                                                                                                                                       <  2:446926/151‑1 (MQ=255)
                     aaCCACATCCAGCACGTCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAgg                                                                                                                               <  4:1227016/151‑1 (MQ=255)
                       ccACATCCAGCACGTCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGAt                                                                                                                             <  7:909404/151‑1 (MQ=255)
                        cacaTCCAGCACGTCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGAt                                                                                                                             >  3:1743053/1‑150 (MQ=255)
                          caTCCAGCACGTCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATaaa                                                                                                                          <  2:957609/151‑1 (MQ=255)
                            tCCAGCACGTCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAgc                                                                                                                        >  1:1828156/1‑151 (MQ=255)
                                 cACGTCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTc                                                                                                                   >  8:24568/1‑151 (MQ=255)
                                     tCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCgg                                                                                                               >  5:679905/1‑151 (MQ=255)
                                       cAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGAt                                                                                                             <  2:593777/151‑1 (MQ=255)
                                       cAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGAt                                                                                                             >  1:96401/1‑151 (MQ=255)
                                            cGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCt                                                                                                        <  8:309110/151‑1 (MQ=255)
                                                aTTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGAtt                                                                                                    <  7:121455/151‑1 (MQ=255)
                                                 ttACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTg                                                                                                   >  1:258108/1‑151 (MQ=255)
                                                  tACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTgg                                                                                                  >  2:1573666/1‑151 (MQ=255)
                                                  tACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTg                                                                                                   <  5:1546042/150‑1 (MQ=255)
                                                    cGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTg                                                                                                <  7:1561496/151‑1 (MQ=255)
                                                     gTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTg                                                                                                >  4:357009/1‑150 (MQ=255)
                                                      tAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGAc                                                                                              >  3:553344/1‑151 (MQ=255)
                                                      tAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGAc                                                                                              >  7:1647626/1‑151 (MQ=255)
                                                      tAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATT‑GTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACg                                                                                             >  2:1447606/1‑151 (MQ=255)
                                                       aGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGAc                                                                                              <  1:1729813/150‑1 (MQ=255)
                                                       aGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACg                                                                                             >  7:143795/1‑151 (MQ=255)
                                                         aTTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTg                                                                                           <  7:184193/151‑1 (MQ=255)
                                                         aTTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTg                                                                                           <  6:524076/151‑1 (MQ=255)
                                                           tctCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGc                                                                                          <  1:540495/148‑1 (MQ=255)
                                                            aTCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCgg                                                                                        <  5:301293/151‑1 (MQ=255)
                                                            aTCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCg                                                                                         <  2:319192/150‑1 (MQ=255)
                                                            aTCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTATAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTCATTGGTGACGTGCgg                                                                                        >  3:778942/1‑151 (MQ=255)
                                                              cGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGAc                                                                                      <  3:1350574/151‑1 (MQ=255)
                                                                   aCCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATaa                                                                                 <  5:1496291/151‑1 (MQ=255)
                                                                     cGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATaaa                                                                                >  2:1722849/1‑150 (MQ=255)
                                                                     cGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGAGCCGACCATaaa                                                                                >  4:777699/1‑150 (MQ=255)
                                                                        tttttGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTc                                                                            >  6:317539/1‑151 (MQ=255)
                                                                         ttttGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCg                                                                           >  4:954878/1‑151 (MQ=255)
                                                                         ttttGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATT‑GTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTc                                                                            <  7:823950/149‑1 (MQ=255)
                                                                         ttttGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGAGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTc                                                                            <  8:722750/150‑1 (MQ=255)
                                                                          tttGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCg                                                                           <  5:1400252/150‑1 (MQ=255)
                                                                             gCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTgg                                                                       <  7:1145120/151‑1 (MQ=255)
                                                                             gCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTg                                                                        <  1:164877/150‑1 (MQ=255)
                                                                              cAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGt                                                                      <  1:140357/151‑1 (MQ=255)
                                                                               aGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGt                                                                      >  7:691565/1‑150 (MQ=255)
                                                                                     gCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAat                                                                >  8:669448/1‑150 (MQ=255)
                                                                                     gCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAat                                                                <  7:1022838/150‑1 (MQ=255)
                                                                                      ccGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCActat                                                              >  7:386620/1‑151 (MQ=255)
                                                                                      ccGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAatat                                                              <  1:1007321/151‑1 (MQ=255)
                                                                                      ccGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAatat                                                              >  4:5332/1‑151 (MQ=255)
                                                                                       cGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGTGGACCATAAAAGTCGCTGGTATCAATATg                                                             <  6:1545247/151‑1 (MQ=255)
                                                                                       cGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATg                                                             <  3:1181329/151‑1 (MQ=255)
                                                                                             aaaTGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGAc                                                       >  4:1480162/1‑151 (MQ=255)
                                                                                             aaaTGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAACTCGCTGGTATCAATATGATTGAc                                                       >  8:1566204/1‑151 (MQ=255)
                                                                                               aTGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGc                                                     >  5:250428/1‑151 (MQ=255)
                                                                                                  cGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCa                                                  <  5:1274717/151‑1 (MQ=255)
                                                                                                    ggCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAAt                                                <  8:1141477/151‑1 (MQ=255)
                                                                                                     gCAACCAGGTTGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATg                                               <  4:1621883/151‑1 (MQ=255)
                                                                                                     gCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATg                                               <  6:842766/151‑1 (MQ=255)
                                                                                                          cAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAgc                                          >  1:1349277/1‑151 (MQ=255)
                                                                                                            ggATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAgcgc                                        >  2:1350789/1‑151 (MQ=255)
                                                                                                            ggATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAgcgc                                        <  5:1642657/151‑1 (MQ=255)
                                                                                                              aTGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCt                                      >  3:975327/1‑151 (MQ=255)
                                                                                                              aTGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCt                                      >  8:1379173/1‑151 (MQ=255)
                                                                                                               tGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTc                                     >  7:330120/1‑151 (MQ=255)
                                                                                                                gCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACGATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCCGCGCCTc                                     >  5:543963/1‑150 (MQ=255)
                                                                                                                  gTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACg                                  >  3:1385313/1‑151 (MQ=255)
                                                                                                                  gTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACg                                  <  3:1532072/151‑1 (MQ=255)
                                                                                                                    gctctCGACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACgca                                <  8:887823/150‑1 (MQ=255)
                                                                                                                     ctctCCACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACgc                                 >  6:1038751/1‑149 (MQ=255)
                                                                                                                         ccACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGATGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACg                           <  5:735611/151‑1 (MQ=255)
                                                                                                                         ccACGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACg                           <  4:1339103/151‑1 (MQ=255)
                                                                                                                           aCGCCGCGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACGGt                         <  1:931374/151‑1 (MQ=255)
                                                                                                                              ccgcGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATATAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCGCACGCAGCCCGGTgat                      >  8:1227001/1‑151 (MQ=255)
                                                                                                                              ccgcGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGCCGCCCAATGCCAGCGCCTCACGCAGCACGGTtat                      >  1:291389/1‑151 (MQ=255)
                                                                                                                              ccgcGCACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACGGTtat                      >  1:440797/1‑151 (MQ=255)
                                                                                                                                 cgcACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCCGCACGGTTATAGc                   >  4:724703/1‑151 (MQ=255)
                                                                                                                                 cgcACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACGGTTATAGc                   >  1:771219/1‑151 (MQ=255)
                                                                                                                                 cgaaCCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCTGATAATCTTATTGGTGACGTGCGGAAAATAAAAGGCGCTGGTATCAATATTATTGACGCCCAATGCCAGCGACGCACGCAGCACGGTTATAGc                   <  6:1203397/148‑1 (MQ=255)
                                                                                                                                  gcACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTAGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACCCAGCACGGTTAAAGcc                  >  2:724453/1‑150 (MQ=255)
                                                                                                                                  gcACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCATCGCGGATAATCTGATTCGTGACGTGCGCACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCATCGCCTCACGCAGCACGGATATAGCg                  >  4:1040550/1‑151 (MQ=255)
                                                                                                                                   cACCAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTAACAATATGATTTACGCCCAATGCCAGCGCCTCACGCAGCACGGTTATAGCg                  >  5:1801142/1‑150 (MQ=255)
                                                                                                                                      cAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACGGTTATAGCGACGt              >  2:1523718/1‑151 (MQ=255)
                                                                                                                                      cAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACGGTTATAGCGACGt              >  8:438081/1‑151 (MQ=255)
                                                                                                                                      cAATTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCACCACGGTTATAGCGACGt              >  3:31972/1‑151 (MQ=255)
                                                                                                                                       aaTTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACGGTTATAGCGACGTg             >  2:1482860/1‑151 (MQ=255)
                                                                                                                                       aaTTTTAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACGGTTATAGCGACGTg             >  6:1664274/1‑151 (MQ=255)
                                                                                                                                         ttttAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACGGTTATAGCGACGTGGc           >  6:1339236/1‑151 (MQ=255)
                                                                                                                                          tttAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACGGTTATAGCGACGTGGCg          >  2:1652551/1‑151 (MQ=255)
                                                                                                                                            tAGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACGGTTATAGCGACGTGGCGa         <  2:287403/150‑1 (MQ=255)
                                                                                                                                             aGTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACGGTTATAGCGACGTGGCGAtc       >  6:671943/1‑151 (MQ=255)
                                                                                                                                             aGTGACAATT‑GTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACGGTTATAGCGACGTGGCGAtct      <  1:1444714/151‑1 (MQ=255)
                                                                                                                                              gTGACAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATAAATATGATTGACGCCCAATGCCAGCGCCTCACCCAGCACGCTTATAGCGACGTGGCGAtcg      >  7:473164/1‑150 (MQ=255)
                                                                                                                                                 aCAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCCCGCAGCACGGTTATAGCGACGTGGCGATCTCgg   >  8:1459258/1‑150 (MQ=255)
                                                                                                                                                 aCAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACGGTTATAGCGACGTGGCGATCTCGt   >  1:837080/1‑151 (MQ=255)
                                                                                                                                                  cAATTGGTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACGGTTATAGCGACGTGGCGATCTCGTg  <  1:1584977/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                 
CCCCATCATAACGCGCAGGTTAACCACATCCAGCACGTCCAGCCCGAGATTACGTAGATTATCGTGCACCGCTTTTTGCAGCTCTGCCGGAGAAAATGCGGGCAACCAGGATGCGTCCTCTCCACGCCGCGCACCAATTTTAGTGACAATT‑GTCAGGTCGTCAGAGTAAGGATAAAGCGCTTCGCGGATAATCTGATTGGTGACGTGCGGACCATAAAAGTCGCTGGTATCAATATGATTGACGCCCAATGCCAGCGCCTCACGCAGCACGGTTATAGCGACGTGGCGATCTCGTG  >  DH1/2403868‑2404163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑