Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,111,641 C→T 6.4% A260V (GCT→GTT)  opgH → OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,111,6410CT 6.4% 218.0 / 2.8 78A260V (GCT→GTT) opgHOPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist
Reads supporting (aligned to +/- strand):  ref base C (34/37);  new base T (3/2);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

CGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGA‑TGGAGCTTATCGCTGAAGTCGGTGGCGAA  >  NC_000913/1111494‑1111789
                                                                                                                                                   |                                                                                                                                                     
cGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCt                                                                                                                                                      <  1:390005/149‑1 (MQ=255)
 ggCGTTAATGTGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATACCTATATGTAACGAAGACGTGAACCGTGTTTTTGCTg                                                                                                                                                     >  1:1068744/1‑149 (MQ=255)
 ggCGTTAATGGGCATCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATAGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTg                                                                                                                                                     >  1:1237168/1‑149 (MQ=255)
   cGTTAATGGGCTTCCTGCAACTGCTTATTAGTCGCGAAAAATACAGTATATCTGCGTCAACAGCTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGc                                                                                                                                                   >  1:1176957/1‑149 (MQ=255)
    gTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGTTGGcc                                                                                                                                                  >  2:1776330/1‑149 (MQ=255)
    gTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGTTGGcc                                                                                                                                                  >  2:1775893/1‑149 (MQ=255)
          gggCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGCTGAACCATTAAACCCGGAGCATCGCACGGCGTTGAGCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTg                                                                                                                                            >  1:638179/1‑149 (MQ=255)
                 cTGCAACTCCTTATTGGTCGCGATAAATACAGTACATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTg                                                                                                                                     <  1:105944/149‑1 (MQ=255)
                     aaCTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGaa                                                                                                                                 >  1:296840/1‑149 (MQ=255)
                     aaCTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAACATCGCACTGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGATTTTGCCGGCCTGCGTGCAACGTgggga                                                                                                                                 >  2:523870/1‑147 (MQ=255)
                     aaCTGCTTATGGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGaa                                                                                                                                 <  1:1414974/149‑1 (MQ=255)
                      aCTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGCGTTTCTGCTGGCCTGCGTGCAACGTGGGAAt                                                                                                                                <  1:714444/149‑1 (MQ=255)
                        tGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGTGAATCa                                                                                                                              <  2:1568159/149‑1 (MQ=255)
                        tGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCa                                                                                                                              >  2:1626672/1‑149 (MQ=255)
                        tGCTTATTGGTCGCGATAAATACAGCATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGACCCGCGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCa                                                                                                                              <  1:1152757/149‑1 (MQ=255)
                        tGCTTAGTGGTCGCGATAAATACAGGATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCa                                                                                                                              <  1:196631/149‑1 (MQ=255)
                         gCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAg                                                                                                                             <  1:211566/149‑1 (MQ=255)
                              ttGGTCGCGATAAATACAGTATTGCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTGACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTaaaa                                                                                                                        <  2:1176957/149‑1 (MQ=255)
                              ttGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTaaaa                                                                                                                        <  2:1294048/149‑1 (MQ=255)
                               tGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGGAagag                                                                                                                       >  2:1743261/1‑149 (MQ=255)
                               tGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCAGCGTGCAACGTGGGAATCAGTAAAAg                                                                                                                       >  2:1005255/1‑149 (MQ=255)
                               tGGTCGAGATGAATACAGTATCTCTGCGGCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAg                                                                                                                       <  1:1768118/149‑1 (MQ=255)
                                  tCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGGTGATCATGCCTATCTGTAACGGAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCACCGCGGGAAGCAGTgaagaccg                                                                                                                    >  2:416175/1‑144 (MQ=255)
                                  tCGCGATAAATACAGTATATCTGCGTCAACAATTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTG‑TTTTGCTGGCCTGCGTGCAACGGGGGAACCCCGAAAAGCCAc                                                                                                                   >  2:792912/1‑149 (MQ=255)
                                       aTAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCggg                                                                                                               <  1:1154358/149‑1 (MQ=255)
                                           aTACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTAGCTGGCCTGCGTCCACCGTGGGAATCAGGAAAAGCCACCGGGAATg                                                                                                           >  2:92164/1‑149 (MQ=255)
                                                gTATATCTGCGTCAACGGATGGCCATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGGAACGAAGACGTGACCCCCGATTGTGGGGTCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCaaa                                                                                                      <  1:356121/149‑1 (MQ=255)
                                                gTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTGTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCaaa                                                                                                      >  2:1082112/1‑149 (MQ=255)
                                                      cggCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACttt                                                                                                <  2:1752414/147‑1 (MQ=255)
                                                      cTGCGTCAACTGTTCGCGATGACCCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTAGTTATAAAAAACTGCTTGCAATTTGGGAGTCAGTAAAAGCCACCGGGAATGCCAAACACttt                                                                                                <  2:1654650/149‑1 (MQ=255)
                                                      cTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCAGGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACttt                                                                                                <  2:1197705/149‑1 (MQ=255)
                                                         cGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGAt                                                                                             <  1:843009/149‑1 (MQ=255)
                                                          ggcAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTGGATCATGCCTATCTGTAACGAAGACGTGAACCGGGTTTTTGCTGGCCGGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATg                                                                                            <  2:906407/147‑1 (MQ=255)
                                                           ccAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGt                                                                                           <  1:978595/148‑1 (MQ=255)
                                                            cAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTc                                                                                          <  2:1736501/149‑1 (MQ=255)
                                                               cAGTGGGCGATGGACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCAGCGGGAATGCCAAACACTTTGATGTCTAc                                                                                       <  2:1670961/149‑1 (MQ=255)
                                                                aGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCGTGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACa                                                                                      >  1:1561115/1‑149 (MQ=255)
                                                                aGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACa                                                                                      <  1:674351/149‑1 (MQ=255)
                                                                  ttGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACAtt                                                                                    >  2:1499511/1‑149 (MQ=255)
                                                                       gATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACAttgctag                                                                               >  2:1438884/1‑144 (MQ=255)
                                                                       gATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGAGAACCGTGTGTTTGCTGTCCTGCGTGCACCGTGGGAATGAGTAAAAGCCACCGGGAATGGCAAACACTTTGGTGGCcacagtacggg                                                                               >  2:292644/1‑142 (MQ=255)
                                                                        aTGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAAGACTTTGATGTCTACATTGTTAgg                                                                              >  1:976778/1‑148 (MQ=255)
                                                                        aTGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGt                                                                              <  1:228130/149‑1 (MQ=255)
                                                                        aTGAACCATTAAACCCGCAGCACCGCACGGCGTTCATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGt                                                                              <  1:455204/149‑1 (MQ=255)
                                                                            aCCATTAAACCCGGAGCATCGCACGGCGTTGATCATGGCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACa                                                                          >  1:1174777/1‑149 (MQ=255)
                                                                            aCCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACa                                                                          <  2:1126942/149‑1 (MQ=255)
                                                                                ttAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTACCGACGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACCCTTTGCTGTCTACCGTCTTCGTGCCCGTta                                                                      >  2:1701812/1‑149 (MQ=255)
                                                                                ttAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTta                                                                      <  2:914459/149‑1 (MQ=255)
                                                                                   aaCCCGGAGCAGCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTTGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATaa                                                                   <  1:443969/149‑1 (MQ=255)
                                                                                     cccGGAGCATCGCACGGCGTTGATCCTGCCTATCTGTAACGTAGACCAAGGCCGTTTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAAcc                                                                 <  1:1771822/149‑1 (MQ=255)
                                                                                              tCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGCATGCCAAACACTTTGATGTCTCCATTCTTAGTGACAGTTATAACCCGGATGTCt                                                        >  1:244699/1‑149 (MQ=255)
                                                                                               cGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATCCTCAGTGACAGGTATAACCCGGATATCTg                                                       >  2:331010/1‑149 (MQ=255)
                                                                                                  aCGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGt                                                    <  1:1269033/149‑1 (MQ=255)
                                                                                                   cGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGTTGGCTTGCTTACAACGTGGGAGGCAGTAAAAGCCACCGGGGATGCCGAACACCTTGATGTCGACAGTCTCAGTCACCGGGCTAGCCCGGGTAACTGCGta                                                   >  2:1461163/1‑148 (MQ=255)
                                                                                                     gCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGc                                                 <  1:735339/149‑1 (MQ=255)
                                                                                                       gTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGCTGTCTACATTCTTAGTGACAGTTATAACCCGGCTATCTGCGTCGCat                                               >  1:498530/1‑148 (MQ=255)
                                                                                                        ttGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCaga                                              >  2:1299739/1‑149 (MQ=255)
                                                                                                           aTCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAAGCCGGATATCTGCGTCGCAGCGCa                                           >  1:204461/1‑149 (MQ=255)
                                                                                                            tCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCaa                                          >  1:171750/1‑149 (MQ=255)
                                                                                                             cATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCaaa                                         >  1:811124/1‑149 (MQ=255)
                                                                                                              aTGCCTATCTGTAACGAAGACGTGAACAGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAGAGCCACCGGGAAGGCCAAGCACTTTGATGTCTACATTCTTTGTGACAGGTGTAACCCGGATACGTTCGTGGAAGAGCaaac                                        >  2:1400754/1‑148 (MQ=255)
                                                                                                                  cTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGCATGCCAAACACTTTGATGTCTACATTATTAGTGACAGTTATAACCCGGATAGCTGCGTCACCGAGCAAAAAggc                                    >  2:175744/1‑147 (MQ=255)
                                                                                                                  cTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGcc                                    >  1:750763/1‑149 (MQ=255)
                                                                                                                  cTATCTGTAACGAAGACGTGAACCGTGTTATTGCTGGGCTGCGTGCAACGTGGGAATCAGTAAGAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGGGCACAAAGcc                                    >  1:1176488/1‑149 (MQ=255)
                                                                                                                   ttgcTGTAACGAAGACGTGAACCGTGTTTGTGCTGGCCTGCGTGCAACGTGGGAAGCAGCAAAAGCCACCGGGAATGCCAAGCACCTTGATGTCCAAATTCTTAGTCAAAGTTATACCCCGGACATCTGCGTCGCATAGCAAAAAGCCt                                   <  2:881338/146‑1 (MQ=255)
                                                                                                                    aTCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCtg                                  <  1:102246/149‑1 (MQ=255)
                                                                                                                      cTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCtggg                                >  1:1463849/1‑148 (MQ=255)
                                                                                                                        gTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGACGTCCACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCtgga‑tg                             <  1:62247/149‑1 (MQ=255)
                                                                                                                            cGAAGACGTGAACCGTGTTTTTGTTGGCCTGCGTGCAACGTGGGAATCCGTAATAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGA‑TGGAGc                         <  1:1776330/149‑1 (MQ=255)
                                                                                                                            cGAAGACGTGAACCGTGTTTTTGTTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGA‑TGGAGc                         <  1:1775893/149‑1 (MQ=255)
                                                                                                                             gAAGACGTGAACCGTGTTTTTGCTGTCTTTCTTGCAACGTGGGAATCAGTAAAAGCCACCAGGAATGCCAAACGCTTTGATGTCTACATTCTTACGGACAGTTGTAACCCGGATGTCTGCGTCGCCGCGCGAACAGCCTGGA‑AGGAggt                        >  2:255777/1‑147 (MQ=255)
                                                                                                                               aGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCAAGCTCACATAAAAAAAAGCCTGGA‑TGGAGCTTa                      <  2:1749201/149‑1 (MQ=255)
                                                                                                                               aGACGTGAACCGTGTTTTTGCTGGACTGCGTGCAACGAGGGAATCAGTAAAAGCCACCGGGAATGACAAACACTTTGATGTCTTCAGTCTTAGTGACAGTTATAACCCGTATATCTGCGTCGCAGAGCAAAAAGCCTGGA‑TGGAGCTTa                      <  2:159753/149‑1 (MQ=255)
                                                                                                                                gccGTGAACCGTGTTCTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGA‑TGGAGCTTAt                     <  1:537413/147‑1 (MQ=255)
                                                                                                                                 aCGTCAACCGTGTTTTTGCTGGCCGGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGA‑TGGAGCTTATc                    <  1:830213/149‑1 (MQ=255)
                                                                                                                                      aaCCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTACAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGA‑TGGAGCTTATCGCTGa               <  2:991886/149‑1 (MQ=255)
                                                                                                                                              ttttGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGA‑TGGAGCTTATCGCTGAAGTCGGTg       <  1:1450815/149‑1 (MQ=255)
                                                                                                                                                  gCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGAAGACTACATTCTTAGTGACAGTGATAACCCGGATATCTGCGTCCCAGAGCCAAAAGCCTCGACTGGAG‑TTACCGCTGAAGTCggggcggg   >  2:93816/1‑143 (MQ=255)
                                                                                                                                                   cTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGA‑TGGAGCTTATCGCTGAAGTCGGTGGCGaa  <  1:1005255/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGA‑TGGAGCTTATCGCTGAAGTCGGTGGCGAA  >  NC_000913/1111494‑1111789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑