| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,578,192 | G→T | 5.6% | Q383K (CAA→AAA) | mcrB ← | 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,578,192 | 0 | G | T | 5.6% | 351.1 / 4.4 | 120 | Q383K (CAA→AAA) | mcrB | 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB |
| Reads supporting (aligned to +/- strand): ref base G (60/52); new base T (6/2); total (66/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 123 total aligned reads displayed.
TCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCAGAGAGCGATCGGCAGT > NC_000913/4578044‑4578339 | tCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTTTTCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCCTTTTTTg < 2:1626079/149‑1 (MQ=255) tcggtatcaCAATTTGATTAAGCCATTGGGTATCCGGAGAGGGGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTgg < 1:116794/144‑1 (MQ=255) ccGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTgg < 2:1460685/149‑1 (MQ=255) tgTCTTCACAATTTCATGAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGc < 2:1810312/148‑1 (MQ=255) tcatcaCAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCAt > 2:1529054/1‑149 (MQ=255) atcaCAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATaa > 2:807772/1‑149 (MQ=255) tcaCAATTTCATTAAGCCATTGCGTATCCGGAGAGGAGACATATTCCAACCTTCAGCAGATGTAACTCTGCTCCGGCCGGCGTCATCTCCATCGGAATGTGCCCTGTCTGCTTATGTCCTGGTGCAACTCGTTCATTGTTTGTCAtgat > 2:1787484/1‑146 (MQ=255) cacaATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAg < 1:987946/149‑1 (MQ=255) acaATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAgg > 2:1343244/1‑148 (MQ=255) acaATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGa < 2:1115818/149‑1 (MQ=255) acaATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCGCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAgg > 1:1357022/1‑148 (MQ=255) tttCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCGTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAAACTca > 1:1304964/1‑149 (MQ=255) ttCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTcaa < 2:1606535/149‑1 (MQ=255) tCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTcaac < 1:1020526/149‑1 (MQ=255) gCCATTGCATATCCGGAGAGGGGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATATAGACTCAACAAATGaa < 1:1132282/149‑1 (MQ=255) ccATTGCGTAACCGGAGAGGCGCCATCTTCCAACCCACCGCCGAAGTAGCTATGCCCAACGCGGACTCCCTTCCCAACGATAACGGCCGCCTCCCTGATTTCCTGGTTCCCCTCCTTCATGTTCTGGCATAAAGACTCAACaaaataag > 2:314421/1‑144 (MQ=255) ttGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAACTGACGGtt > 2:842883/1‑149 (MQ=255) tCCGGAGAGGTGCCATCTTTCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGTCATAAAGACTCAACAAATGAAGGGTCTGCttt > 2:210964/1‑149 (MQ=255) tCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCttt > 1:930182/1‑149 (MQ=255) tCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGAGAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAAGGAAGGTTCTTCttt > 2:1319090/1‑149 (MQ=255) agGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAGGGTTCTGCTTTTttcttc > 1:247197/1‑149 (MQ=255) ggTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCa < 1:1515092/149‑1 (MQ=255) tGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGt > 1:1649924/1‑149 (MQ=255) ggCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCGTAAAGACTCCACAAATGAAGTTTCTGCTTTTTTATTCAGt > 2:1524342/2‑149 (MQ=255) ccATCTTCCAACCCACGGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTaa > 2:1466220/1‑149 (MQ=255) tCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGCTTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTaaaaa > 1:551240/1‑149 (MQ=255) tCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATGAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCCGTaaaaa > 2:557492/1‑149 (MQ=255) cTTCCAACCCACAGCAGAAGTAACTATGCCCATTGCGGAATCCTTTCCCAAGTAGAGTGGCCTCTTTGCTGAGTGCCGGGTTCATGTTGTTCATTCTTTGGCATAAAGACTCAACAAATTAAGGTTCTGCTTTTTTATTCAGTaaaaaa < 2:994680/149‑1 (MQ=255) aaCCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCACCAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCg > 1:1228243/1‑149 (MQ=255) cacaGCAGAAGTAACTATGCCCAAAGCGGAATCCTTTCCCAAGGATAGGGGCCTCTTGGCTGCTTTCCTGGTTCGACTCGTTCCTTTTTTGGACTAAGGACTCCACAAATGAAGGTTCTGCTGTTTTATTCAGTAAAAAATTCCGGAAc > 2:899344/1‑149 (MQ=255) gcagAAGTAACTATGCCCCCTGCCGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACtgtg < 1:1768093/149‑1 (MQ=255) gcagAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACtgtg < 2:1683418/149‑1 (MQ=255) cagAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTgtgg > 1:1731613/1‑149 (MQ=255) agAAGTAACTATGCCCAATGCGGTATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAATACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTgtggt < 2:1137777/149‑1 (MQ=255) agAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTgtggt < 1:1338980/149‑1 (MQ=255) aaGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCGTAAAGACTCAACAAATGGAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGgtgt > 2:1148501/1‑149 (MQ=255) aaGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTGCATTTTTTGGCATAACGGTCCCACAAATGACGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGgtgt > 1:1500737/1‑149 (MQ=255) aGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTa < 1:619200/149‑1 (MQ=255) aCTATTCCCAATGCTGAATCCTTTCCCAAGTATAGTGGCCTCTTTGCTGATTTCCTGCTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCaa < 2:1572949/149‑1 (MQ=255) aCTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCaa < 2:152372/149‑1 (MQ=255) tATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCaaaa > 1:1427556/1‑149 (MQ=255) tATGCCCAATGCGGAATCCTTTCCCAAGGATAGGGGCCTCTTTGCTGATTTCCTGGTTCCACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGGGCCTCaaac > 1:1487980/1‑148 (MQ=255) aTGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAAc < 1:445183/149‑1 (MQ=255) gCCCAATGCGCAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCt < 1:844805/149‑1 (MQ=255) ccAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTgg < 1:1500881/149‑1 (MQ=255) cAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTCGTTTGTTCAGTAAAAAATTCCGGAACTGTGGTGTATCAACACCTGGc > 1:141321/1‑149 (MQ=255) cAATGCGGAATCCTTTCCCAAGGATAGTGGCCGCTTTGCTGATTGCCTGGTTCAACTCGATCATTTTTTTTTTTTTTTTCTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAAATTCGGAACTGTGGTGTATCAAACCCTgtc > 2:1335307/1‑147 (MQ=255) gCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTAAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCaa < 1:430040/149‑1 (MQ=255) gAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAatat < 2:1419710/149‑1 (MQ=255) aaTCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATc < 1:704079/149‑1 (MQ=255) aTCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAATACCCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCt < 1:1211375/149‑1 (MQ=255) tCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTa > 1:1042702/1‑149 (MQ=255) tttCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTTTTTTTTTTTTTCAACAAATGCAGGTTCTGATTTTTTATTCAGTAAATGATTCCGGAACTGTGGTGTTTCAAATCGTTGCCCAATACCCATgg > 2:1678807/1‑148 (MQ=255) tttCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAACTTCCGGAACTGTGGTGTATCAAAACCTGGCTCAAGATCTATga > 2:466982/1‑149 (MQ=255) tttCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATgg > 1:462610/1‑148 (MQ=255) tgtCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATga > 1:1071456/3‑149 (MQ=255) attCCCAAGGATAGTGGCCTTTTTGCTGATTTCCTGGGTCAACTCGTTCATTCTTTGGCATAAAGACTCAACAAATGAAGGGTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTAGCAAAACCTGGCTCAATATCTATga < 2:764932/148‑1 (MQ=255) ttCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCA‑TTTTTGGCATGAAGACTCAACAAATGAAGGTTCTGATTTTTTATTCAGTAAAAAATTCCGGAACTGGGGTGTTTCAAAACCTCGCTCAAAATCTATgaaa > 2:604518/1‑149 (MQ=255) tCCCAAGGAAAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATgaaa < 1:589452/149‑1 (MQ=255) aaGGATAGTGTCCTCTTTGCTGATTGCCTGGTTCATCTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATgaaagaaa < 1:337325/149‑1 (MQ=255) aaGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAATAATTGCGGAACTGTGGTGTATCAAAACCTGGATCAATAACTATgaaagaaa > 2:1537619/1‑149 (MQ=255) aaGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATgaaagaaa > 2:1232083/1‑149 (MQ=255) ggATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTTTTTTTATTTCTCAACAAATGAAGGCTCTGCTTTTTTATTCAGTGAAAAATGCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCCATTAAAGCAAAt > 2:269770/1‑149 (MQ=255) ggATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTAGGAAAGAAAAt > 2:691661/1‑149 (MQ=255) aGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCACGATCTATGACAGAAAATCgcc > 1:1750083/1‑147 (MQ=255) aGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGAATAAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAGCCTGGCTCCATATCTATGAAAGAAAATGGtg > 1:1750092/1‑148 (MQ=255) aGTGGCCTCTTTGCTGATTCCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCCGTAGTGGCTTCTTGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTc > 1:1318860/1‑149 (MQ=255) ggCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGc < 1:1386712/149‑1 (MQ=255) ctctTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGTCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAAACTGTCTCAATATCTATGAAAGAAAATCGTCTGCGTa > 2:1021972/1‑149 (MQ=255) tctTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTTTTTTTTTTTTTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAATAAGTCCGGAACTGTGGTGTTTCCAAACCTTGCTCAATATCTATGAAAGCAAATCGTCTGCGTAg > 2:1623863/1‑149 (MQ=255) ctTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAgg > 2:809562/1‑149 (MQ=255) tttGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTACAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAggg > 1:726417/1‑149 (MQ=255) tttGATGATTTCCTCGTTCATCTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAggg < 2:453999/149‑1 (MQ=255) ttGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGc < 1:693835/149‑1 (MQ=255) tGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAGGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGACCTGTGGTGCACCAGAACCTGGCGCAATATCTATGAAAGAAAATCGTCTTGGTAGGGCa > 1:592279/1‑149 (MQ=255) gCTGATTTCCTGGTTCAACTCGTTCA‑TTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATa < 1:296125/149‑1 (MQ=255) cttaTTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGTCTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATa < 2:250325/146‑1 (MQ=255) cTGGTTTCCTGGTTCAACTCGTTCATTTTTTGGCGTAAAGACCCAACAAATGAAGGTTCTGCTTTTCTATTGAGTAAACAAATCCGGACCTGTGGTGTATCAAATCCTGGCCCAATATCTATGAAAGAAAATCGTCTGCGTAGAGCATa < 2:615633/149‑1 (MQ=255) cTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATa < 2:679307/149‑1 (MQ=255) cTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATa < 1:807772/149‑1 (MQ=255) cTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTCTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATa < 1:795023/149‑1 (MQ=255) tGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATAg < 2:1104698/149‑1 (MQ=255) tGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAAACATCTCCGTAGCGCATAg > 2:170158/1‑149 (MQ=255) gagttCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCTTTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTAGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGCGCGTAGt > 2:1020395/4‑149 (MQ=255) ggTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCTTCTGCGTAGGGCATAGTCAACAACg < 1:1011469/149‑1 (MQ=255) gTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTCAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATAGCTATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACgg > 2:45312/1‑149 (MQ=255) ttCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGc < 1:616476/149‑1 (MQ=255) cAACGCGTTCATTTTTTTGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGcaa < 2:1381614/149‑3 (MQ=255) aCTCGTTCATTTTTTGGCATAAAGACTCGGCAAATGAAGGTTCTGCTTTTTTATTCAGTAAAACATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGGAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCaga > 1:1178730/1‑149 (MQ=255) aCTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCaga > 1:749510/1‑149 (MQ=255) aCTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCaga > 1:47097/1‑149 (MQ=255) ttCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCAGAGAGCg > 1:1432288/1‑149 (MQ=255) ttCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCAGGAAATGTGGAGTATCAAACCCTGGCTCAATATCTATGACAGAAAATCGTCTGAGTAGGGCAAAGTCGACAACGGCCagagagga > 2:863479/1‑147 (MQ=255) cATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATAGTAAAAACCGGCCAGAGAGCGAt > 1:683037/1‑149 (MQ=255) gttttttGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCAGAGAGCGATc < 2:930182/148‑1 (MQ=255) aTTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTTTTTTTTTTTAAATTCCGGAACTGTGGTGTATTAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTATGCCTTATTTTACAACGGCCAGAGAGCGATc > 2:443176/1‑149 (MQ=255) aTTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCAGAGAGCGATc < 1:280116/149‑1 (MQ=255) aTTTCTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCAGAGAGCGATc < 1:1044899/149‑1 (MQ=255) tttttGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCAGAGAGCGATCgg > 2:1467773/1‑149 (MQ=255) tGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCAGAGAGCGATCGGCAGt < 1:949276/149‑1 (MQ=255) | TCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCATCTTCCAACCCACAGCAGAAGTAACTATGCCCAATGCGGAATCCTTTCCCAAGGATAGTGGCCTCTTTGCTGATTTCCTGGTTCAACTCGTTCATTTTTTGGCATAAAGACTCAACAAATGAAGGTTCTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCAGAGAGCGATCGGCAGT > NC_000913/4578044‑4578339 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |