Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,836,954 G→A 9.4% M1M (ATG→ATA) † setC → putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,836,9540GA 9.4% 370.1 / 8.4 136M1M (ATG→ATA) †setCputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (56/64);  new base A (3/11);  total (59/77)
Fisher's exact test for biased strand distribution p-value = 9.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

ATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTCCTATAA  >  NC_000913/3836806‑3837102
                                                                                                                                                    |                                                                                                                                                    
  aatCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCa                                                                                                                                                    <  2:916585/149‑1 (MQ=255)
  aatCTCAAATGCTGGCCCCAAAATATTGCGCTAGCATTACATTCTCGATGTATCAGCGATACTCAAAAATTCTTAACACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCa                                                                                                                                                    <  1:1524859/149‑1 (MQ=255)
    tctcAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTTTTTCGGTTTTTCAGAAGAATGCaaa                                                                                                                                                  >  2:1424378/1‑149 (MQ=255)
      tcAATTGCTGGCCCTATAATATTTCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCaaaaa                                                                                                                                                >  2:1235828/1‑149 (MQ=255)
      tcAATTGCTGGCCCTATAATATTGCGCTCGCATTGCTTTCTGGTTGTATCGGCGATTCTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTCTTTGTAACTCAGGTATAGTTTTTCGCCCGGGGCTTCAGGAGAATGCaaaaa                                                                                                                                                >  2:1187099/1‑149 (MQ=255)
        aaTTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAAc                                                                                                                                              <  1:384474/149‑1 (MQ=255)
        aaTTGCTGGCCCTATAATATTGCGCTAGCATGGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAAc                                                                                                                                              <  1:456949/149‑1 (MQ=255)
          ttGCCGGCCCCATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACgg                                                                                                                                            <  1:590374/149‑1 (MQ=255)
              tGGCTCTTTAATATTGCGCTAGCATTGCTTTCTGGTTGTATCGGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTAc                                                                                                                                        <  1:463986/149‑1 (MQ=255)
              tGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCAGGTGATTCAGAAGAATGCAAAAAACGGCTAc                                                                                                                                        >  1:1599242/1‑149 (MQ=255)
                tcccTATTATATTGCGCTAGCATTGCTTTCTAAAAATAACAACGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCACTTATAGTTTTTCGCCCGGTGATTAAAAAAAAAAAAAAAAACGGCTACCa                                                                                                                                      <  2:1655936/148‑1 (MQ=255)
                   cTATACTATTGCGCGAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTc                                                                                                                                   <  1:773165/149‑1 (MQ=255)
                   cTAGAATATTGCGCTAGAATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCCCTc                                                                                                                                   <  2:161845/149‑1 (MQ=255)
                     ataataTTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCCGAAGAATACAAAAAACGCCTACCACTCCa                                                                                                                                 >  2:181600/1‑149 (MQ=255)
                     ataataTTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCa                                                                                                                                 >  2:722744/1‑149 (MQ=255)
                       aataTCGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAAAATGCAAAAAACGGCTACCACTCCATc                                                                                                                               <  2:280671/149‑1 (MQ=255)
                         tatTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTAATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTAGCCCGGTGATTCAGAAGAATACAAAAAACGGCTACCACTCCATCaa                                                                                                                             >  1:1098523/1‑149 (MQ=255)
                          atTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGACTGCAAAAAACGGCTACCACTCCATCaaa                                                                                                                            <  1:965985/149‑1 (MQ=255)
                           ttGCGCTAGCATTGCTTTCTGGATGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCaaaa                                                                                                                           >  2:1295548/1‑149 (MQ=255)
                            tGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCGGAAGAATGCAAAAAACGGCTACCACTCCATCaaaaa                                                                                                                          >  1:1234310/1‑149 (MQ=255)
                            tGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCaaaaa                                                                                                                          <  1:624629/149‑1 (MQ=255)
                               gcTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATAc                                                                                                                       >  1:1195522/1‑149 (MQ=255)
                                 tagaactGCTTTCTGTCTGTCTCAACGATACTCAAAAATTCGTCATACCATAATTAGTAGACTGGTAATTATTTGTATTTGAGTTATATTTTTTTGTCCGGGTATAAAAAAGTAATTAAAAAACGGCTACCACTCCATCAAAAATACtt                                                                                                                     <  2:838049/143‑1 (MQ=255)
                                    caattcTTTCTGGTTGTATCAGCAAAACTAAAAAATTCTTAATAAAATACTCATTCGATTGGTACTTATTTGTTGCTACGTTCGGCAGAGCCGCCCTCTGTTTTGAAAAAAAAAAAAAAACGGCTACCACTCCATCAAAAATACTTGAt                                                                                                                  <  2:1739451/144‑1 (MQ=255)
                                     aTTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGAATGATACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGAtc                                                                                                                 <  2:12201/149‑1 (MQ=255)
                                     aTTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACGCTTTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCCCTCCATCAAAAATACTTGAtc                                                                                                                 >  1:1417729/1‑149 (MQ=255)
                                     aTTCCTTTCCGGTTGTATCAGCGATACTCAAAAATTCTTAATAAAATACCAATCCGCCCGCCACTTATTTGTAAGTCAGCTATATTTTTTCGCCCGGTGATTAAAAAAAAAAAAAAAAACGGCTACCACTCCATCAAAAATACTTGAtc                                                                                                                 <  2:1574710/149‑1 (MQ=255)
                                       tGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGAtctc                                                                                                               >  1:32045/1‑149 (MQ=255)
                                          tttCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTTTTTTTTTTTTTCAGAAGAATGCGATAAACGTCTACAACTCCATCAATAATACTTGAtcttgct                                                                                                            >  2:277102/1‑145 (MQ=255)
                                              tGGATGTAACAGCTATACTCAAAAAGTCTTAAGAAAAGACTCATGCGACGAGTACTTATTTGTAACTCACTTATATTTTTTCGCCCGGTGATTAAAAAAAAAAAAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCcg                                                                                                        <  2:694215/149‑1 (MQ=255)
                                               ggTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCcgc                                                                                                       <  1:616130/149‑1 (MQ=255)
                                                gTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTGCGAAGCATGCAAAAAACGGCTACCACTCCATCACCAATACTTGATATCACttgagcg                                                                                                      >  2:1442327/1‑143 (MQ=255)
                                                 ttGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTTTCTTTGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCCCACTGCCGCgg                                                                                                     >  2:133781/1‑149 (MQ=255)
                                                  tGTCTCAGCGATACTCAAAAAGTCTTAATACAATACTCATTCGACTAGTCCTTATTTGTAGCTCAGTTATATTTTTTCGCCCGGTGATTCAAAAAAAAAAAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGc                                                                                                    <  2:1064756/149‑1 (MQ=255)
                                                      tCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCAttt                                                                                                >  1:453805/1‑149 (MQ=255)
                                                       cAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCAtttt                                                                                               <  2:1725502/149‑1 (MQ=255)
                                                        aGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCCGAAGAACACAAAAAGCGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCAttttt                                                                                              >  1:864643/1‑149 (MQ=255)
                                                         gCGATCCTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTAAAAAAAAAAAAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTa                                                                                             <  2:1419670/149‑1 (MQ=255)
                                                         gCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTa                                                                                             <  1:546195/149‑1 (MQ=255)
                                                          cGACACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTAc                                                                                            <  1:967635/149‑1 (MQ=255)
                                                           aaTACTCAAAAATTCTTAGTACAGTACTTTTTCGACTGGTAGTTATTTGTAACTCAGTTATCTTTTTTCGCCCGGTGATTCAGAAGAATGCAATTATCGGCTAAAAAAAAAAAAAAAATACTTGATCTCACTGCCGCGGCATTTTTACt                                                                                           <  2:809669/148‑1 (MQ=255)
                                                              aCTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGt                                                                                        <  1:819851/149‑1 (MQ=255)
                                                              aCTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGt                                                                                        >  1:131984/1‑149 (MQ=255)
                                                               cTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTc                                                                                       <  2:116676/149‑1 (MQ=255)
                                                               cTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTc                                                                                       <  1:1670752/149‑1 (MQ=255)
                                                                tCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCg                                                                                      >  2:918522/1‑149 (MQ=255)
                                                                tCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCg                                                                                      >  2:997662/1‑149 (MQ=255)
                                                                tCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCg                                                                                      >  1:1526613/1‑149 (MQ=255)
                                                                 cAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGTATTTTTACTTGTCGc                                                                                     <  1:722744/149‑1 (MQ=255)
                                                                  aaaaaTTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGcc                                                                                    >  1:1516114/1‑149 (MQ=255)
                                                                   aaaaTTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACACCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCt                                                                                   >  1:93376/1‑149 (MQ=255)
                                                                    aaaTTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCGTCAAAAATACTTGATCTCCCTGCCGCGGCATTTTTACTTGTCGCCtt                                                                                  <  1:1014171/149‑1 (MQ=255)
                                                                     aaTTCTTAATACAATCCTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCttt                                                                                 <  2:922355/149‑1 (MQ=255)
                                                                      ctttatcatacagtacTCATTCGACCGTTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTc                                                                                <  1:1768493/142‑1 (MQ=255)
                                                                            aatacaatacTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTTTCGCCTTTCTGACgg                                                                          >  2:199989/1‑149 (MQ=255)
                                                                            aatacaatacTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACgg                                                                          >  1:81874/1‑149 (MQ=255)
                                                                              tacaatgcTCATCCGACTGGTACTTATTTGTACCTCCGTTACTTTTTGTCTCCCGGTAATACAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGt                                                                        <  2:1541290/149‑1 (MQ=255)
                                                                               acaatacTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTa                                                                       <  1:1767182/149‑1 (MQ=255)
                                                                                  atacTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTg                                                                    >  1:1023025/1‑149 (MQ=255)
                                                                                    cgtCAGTCGACTGGTACTTATTTGGAACTCAGTTATATTTTGTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTTCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCg                                                                  <  2:1147192/147‑1 (MQ=255)
                                                                                     cgcATTCGACTTGTACTTATTTGTAACTCAGTGATATTTTTACGCCCGGTGAATCAAAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCgg                                                                 <  2:778586/147‑1 (MQ=255)
                                                                                      tCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTCTTGCggg                                                                >  1:1662730/1‑149 (MQ=255)
                                                                                      ccATTCGACTGGTACTTATTTCTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTCCCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCggg                                                                <  1:537616/148‑1 (MQ=255)
                                                                                        aTTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGgcg                                                              <  1:271983/149‑1 (MQ=255)
                                                                                            gACTGGTACTTATTTGTAACCCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGctct                                                          <  1:303736/149‑1 (MQ=255)
                                                                                               tGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCa                                                       <  1:509997/149‑1 (MQ=255)
                                                                                                  tACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCCGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTTTCGCCTGTCTGACGGGGATAGCAGGCGCGCTTCAGAc                                                    >  2:1198766/1‑149 (MQ=255)
                                                                                                  tACTTATTTGTAACTCAGTCATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGTGACATTTATTTTTGTCGCCTTTCTTGCTGGTATTGCGGGCGCTCTTCAGAc                                                    >  1:1312753/1‑149 (MQ=255)
                                                                                                    ctcttttGTAACGCAGTTAGAGTTCTTCGCCCGGTGATTAAGAAGAATGCAAAAAACGGCTACCACTCCCTCAAAACTACTTGATCTCACTGCCGCGGCAGTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGCCGCTCTTCAGACTc                                                  <  2:70365/145‑1 (MQ=255)
                                                                                                     ttATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGGGCTCTTCAGACTcc                                                 >  1:600549/1‑149 (MQ=255)
                                                                                                     ttATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTAATTGTCGGCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTcc                                                 >  1:1230352/1‑149 (MQ=255)
                                                                                                        tttGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTAc                                              <  1:968429/149‑1 (MQ=255)
                                                                                                         ttGTAAGTCAGTTATATTTTTTCGCCCGGTGTTTGAAAAAAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTAcc                                             <  2:266658/149‑1 (MQ=255)
                                                                                                         ttGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTAcc                                             <  1:1766869/149‑1 (MQ=255)
                                                                                                             aaCTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCCCTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTaa                                         <  1:1356572/149‑1 (MQ=255)
                                                                                                             aaCTCAGTTATATTCTTTCGCCCGGTCACTCAGAAGAATGCAAAAAACGGATACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTaa                                         <  1:622465/149‑1 (MQ=255)
                                                                                                                tCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGta                                      >  1:820453/1‑149 (MQ=255)
                                                                                                                tCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGAATCCTACCCTAAGta                                      >  2:417100/1‑149 (MQ=255)
                                                                                                                    ttATATTTTTTCGCCCGGTGATTCAGCAGCATGCAATAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGCCGGGTATTGCGGCCGCGCTGCCGACTCCTCCCACAAGtgtctt                                  >  2:837663/1‑144 (MQ=255)
                                                                                                                         tttgtccGCCCGGGGATTCAAAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTCTCGCCTTTCTGACGGGTATTGCGGGCGCTCGTCAGACTCCTACCCTAAGTATATTCCTCg                             <  2:1288916/143‑1 (MQ=255)
                                                                                                                           ttttCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCCGACTCCTACCCTAAGTCTATTCCTCGCa                           >  1:261835/1‑149 (MQ=255)
                                                                                                                            tttCGCCCGGTGATTCAGAAGAATGCACAAAACGGCTACCACACCATCAAAGATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAg                          <  2:1645116/149‑1 (MQ=255)
                                                                                                                                 cccGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGaa                     <  1:722058/149‑1 (MQ=255)
                                                                                                                                 cccGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACGGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATTaa                     >  1:800158/1‑149 (MQ=255)
                                                                                                                                 cccGGTGACTCAGAAAAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGaa                     <  1:1616929/149‑1 (MQ=255)
                                                                                                                                  ccGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAAc                    <  2:174702/149‑1 (MQ=255)
                                                                                                                                  ccGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAAc                    <  1:1424378/149‑1 (MQ=255)
                                                                                                                                   cGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGCTGAACt                   >  2:928058/1‑149 (MQ=255)
                                                                                                                                   cGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACt                   >  2:1797894/1‑149 (MQ=255)
                                                                                                                                   cGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTCCTGACGGGTGTTGCGGGCGCTCTTCAGACTCCTGCCCTAAGTAGATTCCTCGCAGATGAACt                   >  2:1368113/1‑149 (MQ=255)
                                                                                                                                     gTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAAGTGa                 >  1:1641523/1‑149 (MQ=255)
                                                                                                                                     gTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATAAAAGAGAGGTGATCCCACTGAGGGGGCGGTATTACGGGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGa                 >  2:1740781/1‑149 (MQ=255)
                                                                                                                                        agtCAGAAGAATGAAAAAAACGGCTACCACTCCATGAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAg              <  2:417803/147‑1 (MQ=255)
                                                                                                                                          tCAGAAGAATCCAAAAAACGCCTACCACTCCACCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCCCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGcc            <  2:1234310/149‑1 (MQ=255)
                                                                                                                                             gacgaaTGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGATCTGAAAGCCCGt         <  2:668397/146‑1 (MQ=255)
                                                                                                                                              aagaaTGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTc        <  1:406367/149‑1 (MQ=255)
                                                                                                                                              aagaaTGCAAAAAACGGCTACCACCCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTc        <  1:33031/149‑1 (MQ=255)
                                                                                                                                                 aaTGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTCCta     <  1:709687/149‑1 (MQ=255)
                                                                                                                                                  aTGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCCCTGCCGCGGCAGTTTTACTTGTCGACTTTCTGACGGGTCTTGCGGGCGCTCTTCAGACTCCTACCCTACGTATCTTCCTCGCCGAGGAACTGAAAGCCCGTCCtat    >  2:227011/1‑149 (MQ=255)
                                                                                                                                                   tGCAAAAAACGGCTACCACTCCATCACCACTATTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTCCtata   >  2:1290530/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTCCTATAA  >  NC_000913/3836806‑3837102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: