Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,587,084 T→A 5.4% I48N (ATC→AAC)  yhhA → DUF2756 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,587,0840TA 5.4% 420.0 / 2.9 161I48N (ATC→AAC) yhhADUF2756 family protein
Reads supporting (aligned to +/- strand):  ref base T (88/58);  new base A (2/8);  total (92/69)
Fisher's exact test for biased strand distribution p-value = 1.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 164 total aligned reads displayed.

GCGATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCC  >  NC_000913/3586939‑3587232
                                                                                                                                                 |                                                                                                                                                      
gcgATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCa                                                                                                                                                     <  2:1067252/149‑1 (MQ=255)
gcgATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGGATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCa                                                                                                                                                     >  2:285878/1‑149 (MQ=255)
gcgATGAAACGACTTCTGATTCTTACGGCACTCCGGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATccc                                                                                                                                                     >  2:840241/1‑148 (MQ=255)
 cgATGAACCGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAg                                                                                                                                                    <  1:1021060/149‑1 (MQ=255)
      aaaCGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCaaaa                                                                                                                                               >  1:1791418/1‑149 (MQ=255)
      aaaCGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCaaaa                                                                                                                                               >  1:1436928/1‑149 (MQ=255)
        aCGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAg                                                                                                                                             >  2:1484561/1‑149 (MQ=255)
           aCTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCCGCAGCGGATGCAAACCCAGATGCAGACTCAGCCAAACCAGCAAAAAgggt                                                                                                                                          >  2:185293/1‑148 (MQ=255)
           aCTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCCGCAAAAAGGG‑a                                                                                                                                         >  2:1027378/1‑149 (MQ=255)
              gcTGATTCGTCCGGCACTCCTGCCGTTTGGCTGCTTTGCACAGCTCGGTAATACTCGCAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCCGCAGCGGATGCAAGCCCAGATGCAGACTCAGCAAATGCAGCAAAAATGG‑ATGc                                                                                                                                      <  2:482969/148‑1 (MQ=255)
               cTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACCACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCt                                                                                                                                     >  2:1260008/1‑149 (MQ=255)
               cTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGAACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGGATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAAGG‑ATGCt                                                                                                                                     >  1:809752/1‑149 (MQ=255)
                 gATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGa                                                                                                                                   <  1:21627/149‑1 (MQ=255)
                    tCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGGATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATc                                                                                                                                >  2:1552911/1‑149 (MQ=255)
                    tCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGACCATTAATACTATGACCAACCCTAACCGGCCGGGGTATAAGATACCCAGACAGCAGCGAGTTTAAACACAGATGCAAACTCCGCAAGT‑CAGGAACAAGGGAATGCTGaacg                                                                                                                                >  2:505533/1‑147 (MQ=255)
                     cTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCa                                                                                                                               <  1:1020962/149‑1 (MQ=255)
                       tACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGc                                                                                                                             >  1:310099/1‑149 (MQ=255)
                       ctccccccTCCTGCCGTTTGACGACTATGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGAAAAAAAAAAAAAAAGGG‑ATGCTGAATCAGc                                                                                                                             <  2:1266855/142‑1 (MQ=255)
                        ccGGCACTCCTGCCGTTTGTCGTCTTTGCACACTCCATTAATACTCTCAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCTGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCa                                                                                                                            <  1:1651593/148‑1 (MQ=255)
                        aCGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAgac                                                                                                                            >  2:1587907/1‑147 (MQ=255)
                        aCGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCa                                                                                                                            >  1:1345326/1‑149 (MQ=255)
                        aCGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCACCGGATGCAAACCCAGATGCAGACTCAGCCAAGCCAGCAAAAAGGG‑ATGCTGAATCAGCa                                                                                                                            >  2:1104576/1‑149 (MQ=255)
                          ggCACTCCTGCCGTTTGTCGGCTTTTCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTAGCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATTaacaac                                                                                                                          >  1:431133/1‑149 (MQ=255)
                             aCTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGa                                                                                                                       >  1:1576728/1‑149 (MQ=255)
                              cTCCTGCCGTTTGGCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGaa                                                                                                                      <  2:333726/149‑1 (MQ=255)
                                ccTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGAGGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGaaaa                                                                                                                    <  1:372883/149‑1 (MQ=255)
                                   gCCCTTAGTCTGCTGCGCTCAGCCCAGTAATACGTTCAACAACCCGAACCAGCCGGTGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGTTGCAGACTCAGAAAAAAAAAAAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGc                                                                                                                 <  2:1705805/149‑1 (MQ=255)
                                      ggttGTCGGCTTTGCACGGCCCATTAATCCTCTGAACAACTGTAACCGGCCGCGGTAGCAGGTACGCAGCCAGCAGCGAATACAAACCCCGATGTATACTTAGAAAAAAAAAAAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCaaa                                                                                                              <  2:1788004/147‑1 (MQ=255)
                                           tCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGGGGCTGCAAACCCGGATGCAGACTCAGCAAATCCAGCAAAAAGGA‑GTGCTGAAACAGCCACGGAAAACCCAAAATCAg                                                                                                         >  2:197551/1‑149 (MQ=255)
                                           tCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAg                                                                                                         >  1:1658822/1‑149 (MQ=255)
                                             ggCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGCTGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCGGAATCAGCAACTGGAAACGCaaaacaagtt                                                                                                       >  2:13217/1‑142 (MQ=255)
                                             ggCTTTGCACAGCCCATTAATACTCAGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAACCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACACAG‑tt                                                                                                      >  2:783363/1‑149 (MQ=255)
                                              gCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGATAACGCAAACTCAG‑TTg                                                                                                     >  2:1214165/1‑149 (MQ=255)
                                              gCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACCCAG‑TTg                                                                                                     >  2:631677/1‑149 (MQ=255)
                                                 ttGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCaa                                                                                                  <  1:1278822/149‑1 (MQ=255)
                                                      caGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAAcagca                                                                                             >  1:1542775/1‑149 (MQ=255)
                                                       aGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑CTGCTGAATCAGCAACTAAAAACGCAAACTCAG‑TTGCAAcagcag                                                                                            >  1:693642/1‑149 (MQ=255)
                                                         cccATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCCGCAACTGAAAACGCAAACTCAG‑TTGCAACAgcagca                                                                                          >  1:233806/1‑149 (MQ=255)
                                                         cccATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACCCAG‑TTGCAACAgcagca                                                                                          >  2:420489/1‑149 (MQ=255)
                                                          ccATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCAt                                                                                         >  2:70653/1‑149 (MQ=255)
                                                           cATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCAtt                                                                                        >  1:956164/1‑149 (MQ=255)
                                                            atgaagaCTCTTAACAACCCAAACCAGCCGGGGTATCAAATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAAAAAAAAAAAAAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCAttt                                                                                       <  2:1774224/143‑1 (MQ=255)
                                                            aTTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCAttt                                                                                       >  2:1298676/1‑149 (MQ=255)
                                                                 tACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGaaa                                                                                  >  1:1470936/1‑149 (MQ=255)
                                                                  acactGAACGACCCTAACCAGCCGGGGTATCAGACACCCACCCAGCAACGGATGCAAACCCAGATGCAGACTCAGAAAAAAAAAAAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGaaaa                                                                                 <  2:1595914/146‑1 (MQ=255)
                                                                  aCTCTGAACAACCCCACCCACCCGGGGTATCACATACCCAGCCAGCAGTGGATGCAAACCTAGAGGCAGACTCAGCAAAGCCAGCTAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGaaaa                                                                                 <  2:803675/149‑1 (MQ=255)
                                                                  aCTCCGAACAACCCGAACCAGCCGCGGTATAAGATACCCAGACACCAGCGGATGCAAACCCAGATGCAGACTTAGCAAATTCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGaaaa                                                                                 <  2:596322/149‑1 (MQ=255)
                                                                   ctcttcacaacCTCAGCCAGCCGGGGTATCAGATTCCCAGCCAGCAGCGGATGCAAACCCAGATGCAGCCCCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCCG‑TTGCAACAGCAGCATTTAGAAAAc                                                                                <  2:45156/143‑1 (MQ=255)
                                                                   ctctGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAAc                                                                                >  2:1449696/1‑149 (MQ=255)
                                                                   ctctGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAAc                                                                                >  1:264759/1‑149 (MQ=255)
                                                                    tctGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑GTGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAAcc                                                                               >  1:1384136/1‑149 (MQ=255)
                                                                    tctGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAAcc                                                                               <  1:1741175/149‑1 (MQ=255)
                                                                        aacaacCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCGGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGTAACAGCAGCATTTAGAAAACCAGAt                                                                           >  1:1113951/1‑149 (MQ=255)
                                                                         acaacCCTAACCCGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATa                                                                          <  1:158306/149‑1 (MQ=255)
                                                                         acaacCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGC‑CCCGCTGCTTGGAACAGCAGCATTTAGAAAACCAGATa                                                                          <  1:883531/149‑1 (MQ=255)
                                                                           aacCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATaaa                                                                        >  2:341295/1‑149 (MQ=255)
                                                                                 aaCCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAAAACAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACaataa                                                                  <  2:292033/149‑1 (MQ=255)
                                                                                   ccAGCCGGGGTATCAGAGACCCAGCCAGCAGCGGATGCAACCCCAAATTCAGGCTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTATAAAACCGGATAAACGATAAtt                                                                <  2:817377/149‑1 (MQ=255)
                                                                                      gCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCCGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATtatc                                                             >  1:1392378/1‑149 (MQ=255)
                                                                                      gCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGGAAAATCCGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTGGG‑TTGCAACAGCAGCTTTTAGAAAACCGGATAACCAATATGtctc                                                             >  1:608198/1‑149 (MQ=255)
                                                                                       ccGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCa                                                            >  1:803274/1‑149 (MQ=255)
                                                                                          gggTATCAGATACCCAGCCAGCCGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAAGCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCg                                                         <  2:1370050/149‑1 (MQ=255)
                                                                                               tCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCGGATAAACATTAATTCTTAGCGGgtgt                                                    >  1:660054/1‑149 (MQ=255)
                                                                                               tCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGgtgt                                                    >  1:1618124/1‑149 (MQ=255)
                                                                                                 aGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAGGGG‑ATGCTGAATCAGCAACTGAAAACGCACACGCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTg                                                  >  2:1192634/1‑149 (MQ=255)
                                                                                                 aGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTg                                                  <  1:1586613/149‑1 (MQ=255)
                                                                                                 aGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTg                                                  >  2:1692219/1‑149 (MQ=255)
                                                                                                   aTACCCAGCCAGCAGCGGATGCAAACCGAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTGGCa                                                <  2:843137/149‑1 (MQ=255)
                                                                                                     aCCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCCGCGGGTTTTGCGGt                                              >  2:737446/1‑149 (MQ=255)
                                                                                                      cccAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTc                                             <  2:1346998/149‑1 (MQ=255)
                                                                                                       ccagccagCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTca                                            <  1:153485/149‑1 (MQ=255)
                                                                                                        cagccagcAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTccc                                           >  2:1670538/1‑147 (MQ=255)
                                                                                                            cagcagCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTAAGCGGGTTTTGCAGTCACAGcc                                       >  1:1132606/1‑149 (MQ=255)
                                                                                                               cagcGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCggg                                    <  1:70653/149‑1 (MQ=255)
                                                                                                                agcCGATGCAAACCCAGATGCAGACTCAGAAAAACCAAAAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCgggg                                   <  2:955410/149‑1 (MQ=255)
                                                                                                                  cGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGa                                 <  1:733312/149‑1 (MQ=255)
                                                                                                                    gATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGc                               <  1:57347/149‑1 (MQ=255)
                                                                                                                       gCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGaa                            <  1:312071/149‑1 (MQ=255)
                                                                                                                         aaaCCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTGAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACTGCCGGGGGAGCGACAt                          >  2:1218314/1‑149 (MQ=255)
                                                                                                                         aaaCCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCAAGTATGAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAAt                          >  1:422548/1‑149 (MQ=255)
                                                                                                                          aaCCCAGATGCAGACTCAGCAAATCAAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAAGAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATc                         <  2:745498/149‑1 (MQ=255)
                                                                                                                             ccAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAGATcccg                      >  2:1604172/1‑149 (MQ=255)
                                                                                                                             ccAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATcccg                      <  2:1499650/149‑1 (MQ=255)
                                                                                                                              cAGATGCAGACTCAACAAATCCAGCAAAAAGGG‑ATGCGGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATcccgc                     <  2:388722/149‑1 (MQ=255)
                                                                                                                                 aTGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATcccgcccg                  <  1:544017/149‑1 (MQ=255)
                                                                                                                                 aTGCAGACTCAGCAAAGCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATcccgcccg                  <  2:510912/149‑1 (MQ=255)
                                                                                                                                      gACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGgcagc             >  1:1468990/1‑149 (MQ=255)
                                                                                                                                         tCAGCAAATCCAGCAAAAATGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCaaa          >  1:924524/1‑149 (MQ=255)
                                                                                                                                         tCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTTCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCaaa          >  1:1691939/1‑149 (MQ=255)
                                                                                                                                         tCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAAACCCGCCCGGCAGCaaa          >  2:1022413/1‑149 (MQ=255)
                                                                                                                                         tCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGAAGCGAAATCCGGCCCGGCAGCaaa          >  1:932504/1‑149 (MQ=255)
                                                                                                                                         tCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCACCGGGTGTTGCAGTCACAGCCGGGGGAGAGAAATGCAGCCCGGCCGCaaa          >  1:454096/1‑149 (MQ=255)
                                                                                                                                             cAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAAtgct      <  2:1470936/149‑1 (MQ=255)
                                                                                                                                              aaaTGCGGCAAATAGGG‑ATGGTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAAtgctg     <  2:403692/149‑1 (MQ=255)
                                                                                                                                               aaTCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAAtgctgc    <  2:1256993/149‑1 (MQ=255)
                                                                                                                                               aaTCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAAtgctgc    <  2:181087/149‑1 (MQ=255)
                                                                                                                                                aTCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGcc   <  1:1195528/149‑1 (MQ=255)
                                                                                                                                                aTCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGcc   <  1:1285924/149‑1 (MQ=255)
                                                                                                                                                 tCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGccc  <  1:670075/149‑1 (MQ=255)
                                                                                                                                                 tCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGccc  <  2:809752/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                      
GCGATGAAACGACTTCTGATTCTTACGGCACTCCTGCCGTTTGTCGGCTTTGCACAGCCCATTAATACTCTGAACAACCCTAACCAGCCGGGGTATCAGATACCCAGCCAGCAGCGGATGCAAACCCAGATGCAGACTCAGCAAATCCAGCAAAAAGGG‑ATGCTGAATCAGCAACTGAAAACGCAAACTCAG‑TTGCAACAGCAGCATTTAGAAAACCAGATAAACAATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCC  >  NC_000913/3586939‑3587232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑