Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,553,175 T→A 5.7% F276I (TTC→ATC)  amiA → N‑acetylmuramoyl‑l‑alanine amidase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,553,1750TA 5.7% 288.8 / 3.5 100F276I (TTC→ATC) amiAN‑acetylmuramoyl‑l‑alanine amidase I
Reads supporting (aligned to +/- strand):  ref base T (37/55);  new base A (2/4);  total (41/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GCATTTGTGGTGTTGAAATCACCGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGA  >  NC_000913/2553028‑2553320
                                                                                                                                                    |                                                                                                                                                 
 cATTTGGCGTGTGGTAATCACCGCCGGTTCCTTCGGTCCTGGTGGAAACCTCGTTTAGCACCAACCCGGAAGA‑AGAACGGCTGTTAGGTACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTc                                                                                                                                                 <  1:1479777/149‑1 (MQ=255)
   tttGTGGTGTTGAAATCACCGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCa                                                                                                                                               <  1:1526098/149‑1 (MQ=255)
    ttGTGGTGTTGAAATCACCGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCAc                                                                                                                                              <  2:853272/149‑1 (MQ=255)
    ttGTGGTGTTGAAATCACCGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCAc                                                                                                                                              <  1:1277886/149‑1 (MQ=255)
       tggtgTTGAAATCACCGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTgg                                                                                                                                           >  2:90571/1‑149 (MQ=255)
          tggtGAAATCACCGTCGGTTCCTTCGGGGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTc                                                                                                                                        <  1:365155/146‑1 (MQ=255)
            ttGAAATCACCGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCCCTGGTTCGa                                                                                                                                      >  2:57813/1‑149 (MQ=255)
             tGAAATCACCGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGAc                                                                                                                                     <  1:1422569/149‑1 (MQ=255)
              gAAATCACCGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACCGGTTCGACa                                                                                                                                    >  2:1571935/1‑149 (MQ=255)
               aaaTCACCGTCGGTTCCTTCGGTGCTGGTGGCAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACaa                                                                                                                                   >  2:1009625/1‑149 (MQ=255)
               aaaTCACCGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACaa                                                                                                                                   <  1:1436261/149‑1 (MQ=255)
                aaTCACCGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAGCGGCTGTTAGGCACGGCGGCGGTTCGTCAGAAAATCGCCACGTCGATTGCTGAAGGCGGGATCAGTGATTTCCACTGGTTCGACagc                                                                                                                                  >  1:1405786/1‑147 (MQ=255)
                aaTCACCGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAAc                                                                                                                                  >  1:571742/1‑149 (MQ=255)
                aaTCACCGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAAc                                                                                                                                  <  1:1458287/149‑1 (MQ=255)
                aaTCACCGTCGGTTCCTTCGGGGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAAc                                                                                                                                  <  2:59958/149‑1 (MQ=255)
                  tCACCGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAAACGCCACCGCGCTTGCTGAAGGCGTGATCAGTTATTTCCACCGGTTCGACAACCa                                                                                                                                >  2:45354/1‑149 (MQ=255)
                     ccGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGaa                                                                                                                             >  2:560421/1‑149 (MQ=255)
                     ccGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATGGCTGAAGGCGTGATCAGTTATGTCCACAGGCACGACAAGCGGaa                                                                                                                             >  2:513087/1‑149 (MQ=255)
                      cGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGaaa                                                                                                                            <  1:1431215/149‑1 (MQ=255)
                        tCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAACCCGACACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGc                                                                                                                          <  1:959026/149‑1 (MQ=255)
                        tCGGTTCCTTCGGTGCTGGTGGAAACCTCGGTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGc                                                                                                                          <  2:688945/149‑1 (MQ=255)
                          ggTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGcac                                                                                                                        <  1:359780/149‑1 (MQ=255)
                            ttCCTTCGGTGCTGGTGGAAACCTCGTATATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTGATTTCCACTGGTTCGACAACCAGAAAGCACAt                                                                                                                      >  1:808475/1‑149 (MQ=255)
                              ccTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCCCTGGTTCGACAACCAGAAAGCACATTc                                                                                                                    >  2:748787/1‑149 (MQ=255)
                              ccTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTc                                                                                                                    <  1:1120144/149‑1 (MQ=255)
                              ccTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTc                                                                                                                    >  1:1575582/1‑149 (MQ=255)
                                ttCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGa                                                                                                                  >  1:23261/1‑149 (MQ=255)
                                ttCGGTGCGGGTGGAAACCTCGTTTATCACCACCCCGGAAGA‑AGAACGGCTGTTAGCCCCGGCGACGTTTGGTGAGAAAACTGCCACAGCGCAGGCTGAAGGCGTGTGCAGATATTTCCACTGGTACGACAACAAGAAAGCACAtctga                                                                                                                  >  2:602845/1‑145 (MQ=255)
                                  cGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCCGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGaaa                                                                                                                >  2:491712/1‑149 (MQ=255)
                                  cGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGaaa                                                                                                                <  1:1104872/149‑1 (MQ=255)
                                   ggTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGaaaa                                                                                                               <  1:951233/149‑1 (MQ=255)
                                      gCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGc                                                                                                            <  2:197813/149‑1 (MQ=255)
                                       cTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCg                                                                                                           >  1:1298188/1‑149 (MQ=255)
                                        gggtggAAACCTAGTTGAACACCAACCCGGAAGA‑AGAACGGCTGCTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACTGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAATAAGCGa                                                                                                          <  2:1776967/148‑1 (MQ=255)
                                          ggggAAACCTCGTTTATCACCAACCCGGAACAGAGAA‑AGCGGGTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGCAAGCACATTCGAAAACGCGATa                                                                                                        >  1:882622/3‑149 (MQ=255)
                                           tggAAACCTCGTTTATCACCAACCCGGGAGA‑AGAACGGATGTTAGGCACGGCGGCGTTTCGTCAGAAAAGCGCCAAAGCAATTGCTGAAGGCGTGGACAGTGATGTCCACTGGTGCGACAACCAGAAAGCACCTTCGGAAAAGCGAGaa                                                                                                       >  2:12342/1‑149 (MQ=255)
                                           tggAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATaa                                                                                                       <  1:113517/149‑1 (MQ=255)
                                           tgcaacccTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAAGCACGGCGGCGATTCGTCCGCAAATCGCCACAGCTATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGTCTACCAGAAAAAAAAAAAAAAAAAGCGATaa                                                                                                       <  2:1316165/143‑1 (MQ=255)
                                            ggAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAg                                                                                                      >  1:1577300/1‑149 (MQ=255)
                                              aaaCCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGtt                                                                                                    <  1:121512/149‑1 (MQ=255)
                                               aaCCTCGTTTATCACCAACCCGGAAGA‑AGAGCGGCGGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCCCTGGTTCGACAACCAGAAAGCACATTCGAACAAGCGATAAGTTa                                                                                                   <  2:1103176/149‑1 (MQ=255)
                                                aCCTCGTTTATCCCCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTGTTTCCACTGGTAACCCATCCTTAAAGCACATTCGAAAAAGCGATAAGTTAt                                                                                                  <  1:436964/149‑1 (MQ=255)
                                                aCCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCTGCGTTTCGTCAGAAAATCGCCACAGTGATTGCTGAAGGCGTGATCAGTTATATCCACTGGCTCGCCCCCCAGTAAGCACATTCGAATAAGCGATAAGTTAt                                                                                                  <  1:1337151/149‑1 (MQ=255)
                                                 ccTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATg                                                                                                 >  1:1810732/1‑149 (MQ=255)
                                                  cTCGTGTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGGCCGGCGGCGTTTCGTCAGAAAAGCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTGCCACTGGTTCGACAACCGGAAAGCACATTCGATAAAGCGCATAGTTATGa                                                                                                >  2:1067882/1‑149 (MQ=255)
                                                   tCGTTTATCACCAACCCGGAAGA‑AGAACTGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGaa                                                                                               >  1:1356168/1‑149 (MQ=255)
                                                   tCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATATCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGaa                                                                                               >  1:1749863/1‑149 (MQ=255)
                                                   tCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATATCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGaa                                                                                               >  1:1749755/1‑149 (MQ=255)
                                                     gtgtATCAGCAACCCGGAAGA‑AGAACGGCCGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGGGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAAc                                                                                             <  1:675264/146‑1 (MQ=255)
                                                     gTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGCAAAAGCGATAAGTTATGAAAc                                                                                             >  1:1315086/1‑149 (MQ=255)
                                                            aCCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGc                                                                                      >  1:72878/1‑149 (MQ=255)
                                                             ccAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATAGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTACCACTAGGTCGACAACCAGAAAGCACATTCGAAAAAGCGCTAAGTTATGAAACCCGACGcc                                                                                     >  2:294386/1‑148 (MQ=255)
                                                             ccAACCCGGAAGA‑AGAACGGCTGTGAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGca                                                                                     <  2:858808/149‑1 (MQ=255)
                                                               aaCCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATGGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGcaca                                                                                   >  2:22831/1‑149 (MQ=255)
                                                                    ggAAGA‑AGAGCGGCTGTTCGGCACGGCCGCGTTGCGTCATAAAATCGCCACAGCGATGGCTGAAAGCCTGACCGTTTCTTGGCAGGTTTTCGGCAACGAGAAAAAAAAAAAAAAAAAGCGATAAGTTATGAAACCCGACGCACACCAgg                                                                              <  2:742316/149‑1 (MQ=255)
                                                                    ggAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGGTTGCTCAGGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAGAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAgg                                                                              >  1:1508948/1‑149 (MQ=255)
                                                                           acaaCGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAAc                                                                        <  1:223212/147‑1 (MQ=255)
                                                                              aCGGCTGTTAGGCACGGGGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGt                                                                     <  1:580200/149‑1 (MQ=255)
                                                                                ggCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACTCCACGCACACCAGGTTAAACAGttt                                                                   <  1:1009625/149‑1 (MQ=255)
                                                                                  cTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCt                                                                 >  1:275055/1‑149 (MQ=255)
                                                                                   tGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTg                                                                <  1:839562/149‑1 (MQ=255)
                                                                                   tGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTg                                                                <  1:838846/149‑1 (MQ=255)
                                                                                    gTTAGGCACGGCGGCCTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGc                                                               <  2:152441/149‑1 (MQ=255)
                                                                                     ttgggCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCt                                                              <  1:623332/146‑1 (MQ=255)
                                                                                      tAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATATCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTc                                                             <  1:1223478/149‑1 (MQ=255)
                                                                                       acgCACGGCGGCGTTTTCTCCGAAAATCGCCACATCGATTGCTGAAGGCTTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCa                                                            <  2:1405163/147‑1 (MQ=255)
                                                                                       aGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTCAAACAGCTTCTGCTCa                                                            >  2:619509/1‑149 (MQ=255)
                                                                                        ggCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCaa                                                           <  1:1425123/149‑1 (MQ=255)
                                                                                        ggCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATGGCTGAAGGCGTTATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCTCATTCGAAAAAGCGATAAGTTATGATACCCGACGCACACCAGGTTAAACAGTTTCTGCTCaa                                                           <  2:147804/149‑1 (MQ=255)
                                                                                         gCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAAc                                                          <  1:90571/149‑1 (MQ=255)
                                                                                          cACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATATCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGTCGTACACCAGGTTAAACAGTTTCTGCTCAAcc                                                         <  2:1749755/149‑1 (MQ=255)
                                                                                          cACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATATCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACACGTCGCACACCAGGTTAAACAGTTTCTGCTCAAcc                                                         <  2:1749863/149‑1 (MQ=255)
                                                                                              ccggggTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACGGGTTCGACAACCATAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCa                                                     <  2:1061293/148‑1 (MQ=255)
                                                                                               cggcgTTTCGTCAGAAAAGCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCGCTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATATGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAg                                                    <  2:594859/149‑1 (MQ=255)
                                                                                                   ttttCGTCTGAAAATCGCCACTGCGATGTCTGAATGCGTGATTAGTTATTTCCACTCGTTCGACACCCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATa                                                <  1:1272898/148‑1 (MQ=255)
                                                                                                   gTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATa                                                <  1:560421/149‑1 (MQ=255)
                                                                                                   gTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATa                                                <  1:1270955/149‑1 (MQ=255)
                                                                                                   gTTTCGTCAGAAAACCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATa                                                <  1:614553/149‑1 (MQ=255)
                                                                                                   gTTTCGGCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTCTTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATa                                                <  1:787603/149‑1 (MQ=255)
                                                                                                         tCAGAAAATCGCCACAGCGATTGGTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGAttt                                          >  1:949987/1‑149 (MQ=255)
                                                                                                          cAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGCCAACCAGAGAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGAtttc                                         >  2:1361241/1‑148 (MQ=255)
                                                                                                           aGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGt                                        <  1:66930/149‑1 (MQ=255)
                                                                                                           aGAAAAACCCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGt                                        <  1:681644/149‑1 (MQ=255)
                                                                                                              aaaTCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTACGACAACCAGAAGGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTcag                                     >  1:1707493/1‑149 (MQ=255)
                                                                                                                       caGCGATTGCTGAAGGGGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAcc                            <  2:39767/149‑1 (MQ=255)
                                                                                                                          cGATTGCTCAAGGCGTGATCAGTTATTCCCACTGGTTCGACAACCCGAAAGCACCTTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCCGGTAAAACAGTTTCTGCTCATCCTTCAGGATACGATTTGTCAGCAGCTGAccgcc                         <  1:257876/149‑1 (MQ=255)
                                                                                                                                cTGAAGGAGAGATCAGTTATTTCCACTGGTTCGACAACAAGAAAGAACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTACAGGATAAGCAAAGAATGTAGCTGACCGCCGTCGAt                   >  2:372522/1‑149 (MQ=255)
                                                                                                                                 tGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAACAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGacg                  >  1:1048328/1‑147 (MQ=255)
                                                                                                                                  gAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATgg                 <  2:431835/149‑1 (MQ=255)
                                                                                                                                   aaGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGgc                <  1:1128354/149‑1 (MQ=255)
                                                                                                                                    aGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGgcg               >  1:647060/1‑149 (MQ=255)
                                                                                                                                      gCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCa             <  1:1207346/149‑1 (MQ=255)
                                                                                                                                         ttggtaGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGaa          <  2:1810732/144‑1 (MQ=255)
                                                                                                                                         tGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGac          >  1:1529374/1‑148 (MQ=255)
                                                                                                                                         tGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATGCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGaa          <  2:77601/149‑1 (MQ=255)
                                                                                                                                          gATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGCCGATGGCGCAGAAt         >  2:269023/1‑149 (MQ=255)
                                                                                                                                           aTCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGACGTCGAGGGCGCAGACtt        >  1:1419271/1‑149 (MQ=255)
                                                                                                                                              aGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGt     <  1:688942/149‑1 (MQ=255)
                                                                                                                                                ttATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAACAACGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCg   >  1:708888/1‑149 (MQ=255)
                                                                                                                                                 tATTTCCACTGGTTCGACAACCAGAAAGCCCATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGa  <  2:705609/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GCATTTGTGGTGTTGAAATCACCGTCGGTTCCTTCGGTGCTGGTGGAAACCTCGTTTATCACCAACCCGGAAGA‑AGAACGGCTGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGA  >  NC_000913/2553028‑2553320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑