| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 349,642 | A→G | 5.8% | intergenic (+70/‑370) | prpB → / → prpC | 2‑methylisocitrate lyase/2‑methylcitrate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 349,642 | 0 | A | G | 5.8% | 253.3 / 3.2 | 97 | intergenic (+70/‑370) | prpB/prpC | 2‑methylisocitrate lyase/2‑methylcitrate synthase |
| Reads supporting (aligned to +/- strand): ref base A (44/43); new base G (6/2); total (51/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAT > NC_000913/349494‑349790 | ggAGCTGTACGAAGCCATCAACTACTACCAGTACAAAGGGAAGCTCGACAACCTGATTGCCCGTAGACAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGa < 2:95068/148‑1 (MQ=38)cGAGCCGTCCGAAACCACCAACTCCTCCCAGTTCGAAGATAAGCCCGACACCCTGTTCGCCCGGAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGa < 2:1012878/149‑1 (MQ=37) gCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTGGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGt < 2:86732/149‑1 (MQ=255) tACGAAAGGATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGGTTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGCGGTGCAc > 1:946455/1‑149 (MQ=255) tACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCAc > 1:1275034/1‑149 (MQ=255) tACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCAc > 1:774275/1‑149 (MQ=255) tACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCAc > 1:1293682/1‑149 (MQ=255) tACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTGGATTGTATTCGACAGCCGATGCCTGATGCGGCGCTGACGCGACTTATCAGGCGTACGAGGTGCAc > 1:643167/1‑149 (MQ=255) aaaGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAACCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGaa < 1:505072/149‑1 (MQ=255) aaaGCATCAACTACCACCAGTACGAACAGAAGCTCGACAACCTGTTTGCCCGTAGCCAAGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGaa < 2:1050714/149‑1 (MQ=255) aTCAACTCCGACCAGTACGAAGAGAAGCTCGACAACCTGTTCGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCTTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTa < 2:897375/149‑1 (MQ=255) tCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGGCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCTATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGATGCACTGAACTGtgg > 2:858323/1‑147 (MQ=255) tCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAg > 2:1533/1‑149 (MQ=255) actactACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTc > 1:185433/1‑149 (MQ=255) ctactaCCCGTACGAACGGAATCTCGACACCCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCCACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCg < 2:871665/149‑1 (MQ=37) cccAGTACGGAGAGACGCTCGACAGCCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGTCAGGCGATGCCTGCTGCGTCGCTGACGCGACTTATCAGGCCTACGCGGTGCACTGAACTGTAGGTCGGATaa < 2:679186/148‑1 (MQ=25) acCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATaa < 2:619310/149‑1 (MQ=255) acCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATaa < 1:262403/149‑1 (MQ=255) acCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGCTGATTGTATTCGACAGCCGATGCCTGATGCGACGCTGACGCGACTAATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATaa > 1:1069388/1‑149 (MQ=255) cAGAACGAAGAGAAGCTCGACAACCTTTTTGCCTGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGa < 1:853754/149‑1 (MQ=255) gTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGCCGGAGAAGACg > 2:1087164/1‑149 (MQ=255) tACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACgc > 2:796064/1‑148 (MQ=255) tACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACgg > 1:552889/1‑149 (MQ=255) tACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACgg > 1:1219725/1‑149 (MQ=255) cGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGAt < 1:858803/149‑1 (MQ=255) cGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGAt < 1:1094834/149‑1 (MQ=255) cGAAGAGAAGCGCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGAt < 1:820220/149‑1 (MQ=255) gAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATg < 1:1287542/149‑1 (MQ=255) aggcaaccTCCACAACCTGTTTCCCCGTAGCCAAGAGAAAAAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGc < 2:733635/142‑1 (MQ=25) agaAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGt < 1:264753/149‑1 (MQ=255) agaAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGt < 1:117217/149‑1 (MQ=255) gaAGCTCGCCAACCTGTTGGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTc < 1:1089353/149‑1 (MQ=255) cTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCCCTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCAt > 1:300246/1‑149 (MQ=255) cTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCAt > 1:942322/1‑149 (MQ=255) cTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCAt > 1:527642/1‑149 (MQ=255) caaCAACCTGTTTGCCCGTAGCCAGGTGAAAAAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCGGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATcc < 1:506760/147‑1 (MQ=255) aaaaCCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGa < 2:873405/147‑1 (MQ=255) aCAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGa < 1:435730/149‑1 (MQ=255) cAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGAc < 1:96034/149‑1 (MQ=255) tGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCg < 2:943767/149‑1 (MQ=255) gTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTTGGATAAGACTGATGGCGTCGCATCCGACAACCGa < 1:654944/149‑1 (MQ=255) tGCCCGTAGCCAGGTGAACTAAAACACGCCCGTTGATTAAACACATGGACCGGTTGCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGc < 1:297765/149‑1 (MQ=17) ccGTCGCCAGGTGAAATAAAAACCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGAT‑AGACGGATGGCGTCGCATCCGACAACCGATGCCTGa < 1:452418/149‑1 (MQ=255) ccGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTg > 1:520774/1‑149 (MQ=255) cGTAGCCAGGTGAACTAAAAAACGCCCGTTGATTGTATCCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGa < 1:452530/149‑1 (MQ=255) cGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGa < 1:1085312/149‑1 (MQ=255) tAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGGTGCCTGATg > 1:282761/1‑149 (MQ=255) tAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATg > 1:431190/1‑149 (MQ=255) tAGCCAGGTGAAATAAAAAACGCCCGTTGATTGGATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATg > 1:947992/1‑149 (MQ=255) ccACGTCAAACAAAAACCGCCCCTTGATTGGATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCCACGACGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATgcgc < 2:754755/149‑1 (MQ=17) aGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccg > 2:1173063/1‑149 (MQ=255) tGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATGGCGTCGCATCCGACAACCGACGCCGCCTGCGCCGCTg > 2:12530/1‑149 (MQ=17) tGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGcgccgcgg > 2:1070848/1‑147 (MQ=255) gaaagaaaAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATCAGACGGAAGGCGGCGCATCCGACCACCGATGCCTGATGCGCCGCTgg > 2:1238923/1‑148 (MQ=18) gAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGa < 2:230869/149‑1 (MQ=255) gAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGa > 1:221589/1‑149 (MQ=255) aTAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGAAAGATGCGACGCTGACGCGACTTATCAGGCCTACGGGGTGCCCTGAACTGTAGGTCGGATAAGGCAGATGGCGTCGCATCCGACAGCCGAGGCCTGATGCGCCGCTGGCGt > 2:222212/1‑149 (MQ=17) gCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa > 1:1116612/1‑149 (MQ=255) gCCCGTTGATTGGATTCGACCGCCGATGCCTGATGCGTCGCTGACGCGACTTAGCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa < 1:69699/149‑1 (MQ=255) ccggTTGATGGTATGCGACAGGCGATGCGTGATGCGAGGCGGACGCGAAGTATCAGGCGTAAGATGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAg < 2:179097/146‑1 (MQ=14) cccGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAg < 1:1204517/149‑1 (MQ=255) gTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTTTCAGGcc > 1:346884/1‑149 (MQ=255) ttGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCGGCCGGAACTTATCGGGCCt > 1:316623/1‑149 (MQ=17) gATTGTATTCGACAGCCGATGCCTGATGCGTCGCAGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTAc < 1:858323/149‑1 (MQ=255) ttGGATTAGACCGCCGAGGCGTTAGGCGGCGCGGGCGCGACGTATCAGGCATCCGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACgg < 1:388460/149‑1 (MQ=14) tGTATTCGACAGCCGCGGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg < 1:1049993/149‑1 (MQ=255) tGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGCCGTGACTTATCAGGCCTACggg > 1:67884/1‑149 (MQ=255) tGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACCTGACTTATCAGGCCGACggg > 1:1015972/1‑149 (MQ=255) tATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGt < 1:1533/149‑1 (MQ=255) aTTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTg < 1:294365/149‑1 (MQ=255) ttCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCgg > 1:817697/1‑73 (MQ=11) ttCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCgg < 2:817697/73‑1 (MQ=11) ttCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATAGCGTCGCATCCGGCAACCGATGCCTGACGCGCCGCTGACGCGGCGTATCAGGCCTCCggggaga > 2:132056/1‑146 (MQ=11) tCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCa < 1:1061703/149‑1 (MQ=255) gACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGCGGTGGACt > 1:907307/1‑149 (MQ=255) gACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTCATGCACCGGGGACGCGACTTATCAGGCCTACGGGGTGGACt > 1:358347/1‑149 (MQ=14) aCAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATCCCTGCTGCGCCACCGACGGAGCTTACCACGCCTCCGGGGTGcccct > 1:195164/1‑145 (MQ=14) aCAGCCGATGCCTGATGCGTCGCAGACGCGACTTATCAGGCCTACGAGGTGCATTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTg > 1:714102/1‑149 (MQ=255) gCCGATGCCTGAGGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAAc < 1:609714/149‑1 (MQ=255) ccGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACt > 1:1121393/1‑149 (MQ=255) cGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTg < 2:520774/149‑1 (MQ=255) ccTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGGTAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTGTCAGGCCTACGGGGTGCACTGAACTGTAGGt > 1:370858/1‑149 (MQ=255) ccTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGGGACTTATCAGGCCCACGGGGGGCACGGCACTGTAggg > 2:864004/1‑148 (MQ=14) gATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCGGGCCTCCGGGGTGCACTGAACTGTAGGTCgg > 1:549058/1‑149 (MQ=255) aTGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGCACGTCGGATCAGACGCATGGCGTCGCATCCGACAACCGATGCCTGATGCGCGGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGa > 1:630181/1‑149 (MQ=14) tgggTCGCTGGAGGGACGTATGAGGCCTACGGGGTGCACTGAACTGTGGGTTGGATAAGACGGATGGGGTCGCATACCACAACCGATGCCTGATGCGTCACTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGAt < 1:554048/146‑1 (MQ=11) tGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGAt < 1:711100/149‑1 (MQ=255) tCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGCTAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGa > 1:94558/1‑149 (MQ=255) tCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTCCGGGGTGCACTGAACTGTCGGCCGGATAAGa > 2:378876/1‑149 (MQ=255) tCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGCATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGAGACTTATCAGGCCTGCGGGGTGCACTGAACTGTAGGTCGGATAAGa > 2:424598/1‑149 (MQ=255) gCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACg > 1:381877/1‑149 (MQ=255) gcgACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGc > 2:1287077/1‑149 (MQ=12) gcgACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGc > 2:1099183/1‑149 (MQ=12) ttATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGGCGGATAAGACGCATAGCGTCGc > 1:270304/1‑149 (MQ=255) tgaccAGGCCTCCGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGc < 1:1170267/145‑1 (MQ=255) aGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCg > 1:981502/1‑149 (MQ=255) ggCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa < 2:947992/149‑1 (MQ=17) ggCCTACGAGGTGCACTGAACGGGAGGGCGGCTAAGACGGATGGCGACGAAAACGACAACCGATGCCTGATGCGGCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGGCGGATAAGACGCATAGCGTCGCATCCGa < 1:1212861/149‑1 (MQ=14) gCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGGCGGATAAGACGCATAGCGTCGCATCCGAc > 2:1274171/1‑149 (MQ=255) aGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAt > 1:1055187/1‑149 (MQ=39) | CGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAT > NC_000913/349494‑349790 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |