Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913	1,978,503	Δ776 bp	100%		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1978503–1979269	1979278	10–776	56 [0]	[0] 56	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1978502	0 (0.000)	50 (0.720)	33/260	NT	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

TGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTAT  >  NC_000913/1979133‑1979421                                                                                                                                                   |                                                                                                                                               aaatgggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgtat                                                                                                                                              >  1:1092825‑M2/147‑149 (MQ=255)    tgggtctttacccacatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAg                                                                                                                                           >  2:479280‑M2/144‑149 (MQ=255)          tttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgccgatcacacaaaaccctcaattacttaacataaatgTataagtgctac                                                                                                                                     >  1:197988‑M2/138‑144 (MQ=255)          tttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATAc                                                                                                                                     >  1:29773‑M2/138‑149 (MQ=255)          tttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAATCATAc                                                                                                                                     >  1:681313‑M2/138‑149 (MQ=255)              cacttttctaagatttttccgatatcgacgcaactgtactcgtcactaaacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACtctt                                                                                                                                 <  2:857658‑M2/16‑1 (MQ=255)               aattatttaatattcttcctaaatcgacgcaactgtcctcgtcactacacgcccatgaaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCCTACttttg                                                                                                                                >  2:364786‑M2/133‑149 (MQ=255)                     ctcagctttttcccaactcgacgcacctgtactcgtcactacacgcacatacaagggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                          <  2:290265‑M2/23‑1 (MQ=255)                     ctaagatttttcctaaatctacgcaactggcctcgtcactacacgcacatacaacggaggggggctgcgattttcaatactgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                          <  2:320599‑M2/23‑1 (MQ=255)                     ctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                          <  2:586902‑M2/23‑1 (MQ=255)                      taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgtttaagatgacacaaaacactcaattccttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                                                                         >  1:946188‑M2/126‑149 (MQ=255)                      taagatttttcctaaatcgacgaaactgtactcgcccctccaggcgcgtccaacgcaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                                                                         <  1:297710‑M2/24‑1 (MQ=255)                          atttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattccttagcataaaggTATAAGTCATACTTTTGTTTTGGGTGTa                                                                                                                     >  1:1001076‑M2/122‑149 (MQ=255)                          atttttcctaaatcgacgcaactgtactcgtcacgacacgcacatacaacttaggagggcttcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGGCATACTTTCTTTTAAGGTGTa                                                                                                                     >  1:44364‑M2/122‑149 (MQ=255)                                         acgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTaaa                                                                                                      <  1:904890‑M2/43‑1 (MQ=255)                                                   actcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcg                                                                                            >  1:218805‑M2/97‑149 (MQ=255)                                                       gtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcaaacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTATTcgctac                                                                                        >  1:984234‑M2/93‑149 (MQ=255)                                                          actacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacgct                                                                                     >  1:94992‑M2/90‑149 (MQ=255)                                                             acacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagc                                                                                  <  1:1192168‑M2/63‑1 (MQ=255)                                                             acacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCGACGCTagc                                                                                  <  2:300937‑M2/63‑1 (MQ=255)                                                                ctctcatacaactgatggtggcagcgattttcaataacgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATATGGTATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaag                                                                               <  1:268970‑M2/66‑1 (MQ=255)                                                                 gcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaggc                                                                              >  1:1171991‑M2/83‑149 (MQ=255)                                                                 gcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaagc                                                                              >  2:1054750‑M2/83‑149 (MQ=255)                                                                 gcacatacaacggaggggggctgcgattttcaataatgcgtgaggcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaagc                                                                              >  2:1283699‑M2/83‑149 (MQ=255)                                                                 gcacacacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaagc                                                                              <  1:154000‑M2/67‑1 (MQ=255)                                                                       acaacggaggggggctgcgatttttaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAAt                                                                        <  1:191190‑M2/73‑1 (MQ=255)                                                                       acaacggaggggggctgcgattttcaataatgcgtgatgcagaacacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAAt                                                                        <  2:671193‑M2/73‑1 (MQ=255)                                                                       acaacggaggggggctgcgattttacataatgcgagatgcagatcacaaagatcactcatttacttaacgtaaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAAt                                                                        <  2:74139‑M2/73‑1 (MQ=255)                                                                              aggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGTTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAg                                                                 <  2:842104‑M2/80‑1 (MQ=255)                                                                                 ggggctgcgattttcaataatgcgtgatgcagatcacataaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaa                                                              <  1:31893‑M2/83‑1 (MQ=255)                                                                                  gggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaat                                                             >  2:740486‑M2/66‑149 (MQ=255)                                                                                  gggctgcgattttcaataaggcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaat                                                             <  1:811480‑M2/84‑1 (MQ=255)                                                                                             ttcaataatgcgtgatgcagatcaaacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATgg                                                  >  2:345776‑M2/55‑149 (MQ=255)                                                                                                  taatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGt                                             <  1:522462‑M2/100‑1 (MQ=255)                                                                                                  taatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGt                                             <  1:521884‑M2/100‑1 (MQ=255)                                                                                                     tgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTgc                                          <  1:522965‑M2/103‑1 (MQ=255)                                                                                                      gcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGTTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTgcg                                         <  1:892254‑M2/104‑1 (MQ=255)                                                                                                        ggcatgcagaacacacaaagcgctcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGCGTGTATTTCAATCTGTTAAAAAGTTTTTCGTTACAAAAAAAAAAAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGca                                       <  2:1263438‑M2/106‑1 (MQ=255)                                                                                                                gatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCaaa                               >  1:68973‑M2/36‑149 (MQ=255)                                                                                                                 atcacacaaaacactcaattacttaacattaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGGGTGTCGCTACGCTAGCAGGCAAAGATGAAACGGGAGTAGTCGAAATGGGATGTGGCGCAGAGTcaaat                              >  1:912215‑M2/35‑148 (MQ=255)                                                                                                                 atcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCaaaa                              >  2:988519‑M2/35‑149 (MQ=255)                                                                                                                 atcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCaaaa                              >  2:1040799‑M2/35‑149 (MQ=255)                                                                                                                    acacaaaacacacaattactaataataaatgTATAAGTCATACTTTTGTTTTGGGTGTTTTGTTATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATaa                           <  1:623460‑M2/118‑1 (MQ=255)                                                                                                                      acaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACt                         >  1:78778‑M2/30‑149 (MQ=255)                                                                                                                      acaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACt                         >  1:375‑M2/30‑149 (MQ=255)                                                                                                                           acactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCg                    <  1:79163‑M2/125‑1 (MQ=255)                                                                                                                           acactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACGCACCg                    <  2:910380‑M2/125‑1 (MQ=255)                                                                                                                                caattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAaat               <  2:1001076‑M2/130‑1 (MQ=255)                                                                                                                                  attacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCTCAGTCAAAATAACTCAccgtaagtga             >  1:36369‑M2/18‑145 (MQ=255)                                                                                                                                  attacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAaataa             >  1:1270626‑M2/18‑149 (MQ=255)                                                                                                                                     acttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAatca          >  1:398057‑M2/15‑149 (MQ=255)                                                                                                                                           cataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCt    <  2:510587‑M2/141‑1 (MQ=255)                                                                                                                                            atcaatgGAGAAGTCATACATTGGTTTTGGGTGTATTTCAAACTGTTAAAAAGTTTTTCGCTACGAAAAAAAAAAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCta   <  2:1219658‑M2/142‑1 (MQ=255)                                                                                                                                            ataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCAtctgagt   >  2:186851‑M2/8‑146 (MQ=255)                                                                                                                                            ataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCta   >  2:495439‑M2/8‑149 (MQ=255)                                                                                                                                             taaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCtat  >  1:1016052‑M2/7‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                               TGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTAT  >  NC_000913/1979133‑1979421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑