Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,116,406	C→T	M272I (ATG→ATA)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,406	0	C	T	96.6%	182.6 / NA	59	M272I (ATG→ATA)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (28/29);  minor base G (2/0);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTA  >  NC_000913/4116264‑4116549                                                                                                                                               |                                                                                                                                                catCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTCCTGCCGTAACCGCTTTCTCGCCAGTGTTCATCCGGATAAag                                                                                                                                           >  2:248940/1‑149 (MQ=255)    cgcgCAGCCACTGAATGGATGCGCCTGCCATAAACCCCGCACCTTCCAACGCATAGTGCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAagcag                                                                                                                                        <  2:296146/149‑1 (MQ=255)          gCCACTGAATGGATGCGCCTGCCATAAACCCCGCACCTTCCAACGCATAGTTCCCTTCGCCAGTCGGGCCGCAGGCGGTGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTg                                                                                                                                  <  2:90487/149‑1 (MQ=255)               tGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGGGTTCATCAGTGTAACGCACCCAGTGccgaa                                                                                                                             >  2:105876/1‑146 (MQ=255)                gAATGGCTGCGCCCGCCATGACCCCCGCACCTTCCTACGCATAGTTCACTGCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAg                                                                                                                            <  2:666383/149‑1 (MQ=255)                gAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAg                                                                                                                            <  2:793320/149‑1 (MQ=255)                              gCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTAAGCAGACCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCAt                                                                                                              >  1:139146/1‑149 (MQ=255)                                 aTAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATccc                                                                                                           >  1:653440/1‑149 (MQ=255)                                  tAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCct                                                                                                          >  2:225485/1‑149 (MQ=255)                                  tAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGGTCTTCGCCATCCct                                                                                                          >  1:280066/1‑149 (MQ=255)                                   aaaCACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCctt                                                                                                         >  1:298412/1‑149 (MQ=255)                                     acacCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTCCGCCATCCcttct                                                                                                       >  1:606513/1‑149 (MQ=255)                                       acCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCttt                                                                                                     >  2:191978/1‑149 (MQ=255)                                              cTTCCAACGCATAGTTCACTTCGCCAGACGGGCCGCAGGCGATGTGGGGCAACAGGCCGATGTCTGATTTAACCGCTTTCTCGCCAGTGTCGATCAGGATAAAGCAGCATGTGCCAAAGGCTTTTGACGCCATCCCTTCTTTCACGcac                                                                                              >  2:336159/1‑149 (MQ=255)                                              cTTCCAACGCATAGTCCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGcac                                                                                              <  1:353612/149‑1 (MQ=255)                                                 ccAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAAc                                                                                           >  1:205506/1‑149 (MQ=255)                                                  cAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACt                                                                                          >  2:386400/1‑149 (MQ=255)                                                   aaCGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTGCTTTCCCGCCCCACTg                                                                                         >  1:219923/1‑149 (MQ=255)                                                    aCGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGa                                                                                        <  1:447583/149‑1 (MQ=255)                                                    aCGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTCCACGCACAACTGa                                                                                        >  1:275100/1‑149 (MQ=255)                                                         ttgTTCACTTCGCCAGTCGGGCCGCAGGGGATGGTGGTCAGCAGGCCGTTTTCTGATTTCCCCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCaaa                                                                                   <  1:274186/147‑1 (MQ=255)                                                         tAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCCGTATAAAGCAGCCTGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCaaa                                                                                   <  1:562969/149‑1 (MQ=255)                                                         tAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCaaa                                                                                   >  2:184682/1‑149 (MQ=255)                                                         tAGTTCACTGCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGTCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCaaa                                                                                   <  1:346845/149‑1 (MQ=255)                                                                ctgcGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCATTCTCGTCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGcgcg                                                                            <  1:597105/146‑1 (MQ=255)                                                                cTTCGCCAGTCGGGCCGCCGGCGATGGTGGTCAGCAGGCCGTCTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGcgcg                                                                            <  1:259223/149‑1 (MQ=255)                                                                  tCGCCAGTCGGGCCGCAGGGGAGGGTGTTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGc                                                                          <  2:648939/149‑1 (MQ=255)                                                                    gCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCct                                                                        >  1:351613/1‑149 (MQ=255)                                                                         tCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTgg                                                                   <  1:386400/149‑1 (MQ=255)                                                                             gCCGCAGGCGATGTTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCAc                                                               <  1:384042/149‑1 (MQ=255)                                                                               cGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCg                                                             <  1:195986/149‑1 (MQ=255)                                                                                   ggCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGa                                                         >  2:415545/1‑149 (MQ=255)                                                                                       aTGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCTGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACTGCGCGGCCTGCTGGTCACCGGCGATccc                                                     <  2:617416/149‑1 (MQ=255)                                                                                       aTGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATccc                                                     >  1:791715/1‑149 (MQ=255)                                                                                         ggtggtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTTACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCgg                                                   <  2:298412/149‑1 (MQ=255)                                                                                          gtggtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGga                                                  <  2:509130/149‑1 (MQ=255)                                                                                          ggggtTAGCGGGCCGTTTTCCGATGTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCTCTACCTTCTTCCTCCCACAACTCACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGga                                                  <  2:378260/147‑1 (MQ=255)                                                                                             gtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATCGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGAt                                               >  2:706276/1‑149 (MQ=255)                                                                                              tCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGGGAtt                                              >  1:125815/1‑149 (MQ=255)                                                                                                     gtcGTTTTCCGATTTCCCCGCTTTCTCCCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATAc                                       <  2:139146/147‑1 (MQ=255)                                                                                                       cGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATAcgc                                     >  1:463577/1‑149 (MQ=255)                                                                                                        gTTTTCTGATTTCACCGCTTCCTCGCCAGCGTTCATCAGTATCAAGAAGACAGTGCCCTAGCTATTCTTCCCACTCCCTTTCTTATCGAGCACCACACCGCACAGCACGGCCTGCTGCGCACCTGCGATCCCGCAGATTTGAATAcgcg                                    >  2:681081/1‑149 (MQ=255)                                                                                                            tCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTACAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGTCGATTCCGGAGATTGGAATACGCGTgcc                                <  1:535071/149‑1 (MQ=255)                                                                                                             cTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATTCCGGAGATTGGAATACGCGTgccg                               <  1:413450/149‑1 (MQ=255)                                                                                                               gATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTgccgcc                             >  2:666484/1‑149 (MQ=255)                                                                                                               gATTTCACCGCTTGCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTgccgcc                             <  2:122801/149‑1 (MQ=255)                                                                                                                aTTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGACATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGCTTGTAATACCCGTGCCGCCt                            >  2:700811/1‑149 (MQ=255)                                                                                                                  ttCCCCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCCCGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCttt                          <  1:484371/149‑1 (MQ=255)                                                                                                                     aCCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCAttacgc                       >  2:814536/1‑145 (MQ=255)                                                                                                                      ccGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAACGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTgccc                      >  1:30673/1‑148 (MQ=255)                                                                                                                       cGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTCTCACGCCCAACTGACCAAACAGCGCGGCCAGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTaccgc                     <  2:772370/149‑1 (MQ=255)                                                                                                                             ctcGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGt               >  1:333424/1‑149 (MQ=255)                                                                                                                             ctcGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCGTTgccgcccccgc               >  2:407176/1‑144 (MQ=255)                                                                                                                              tcGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCgg                                                            >  1:196551/1‑103 (MQ=255)                                                                                                                              tcGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCgg                                                            <  2:196551/103‑1 (MQ=255)                                                                                                                                 ccAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGt           <  1:642483/149‑1 (MQ=255)                                                                                                                                       ttCATCAGTATCAGGCAGCCAGTGCCATAGGTGTTCTTCGCCACCCCTTCTTTCACGCACAACTGCCCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAcc     <  1:87284/149‑1 (MQ=255)                                                                                                                                         catcaGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTAACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGt   >  2:669893/1‑149 (MQ=255)                                                                                                                                          atcaGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGta  <  1:682257/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                CATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTA  >  NC_000913/4116264‑4116549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑