| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,800,575 | G→A | 17.3% | Q22* (CAG→TAG) | infC ← | translation initiation factor IF‑3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,800,575 | 0 | G | A | 17.3% | 44.5 / 2.8 | 23 | Q22* (CAG→TAG) | infC | translation initiation factor IF‑3 |
| Reads supporting (aligned to +/- strand): ref base G (8/11); new base A (2/2); total (10/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
ATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATCACTTCATTTACGTCC > NC_000913/1800432‑1800714 | aTCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGgcgc < 1:436703/149‑1 (MQ=255) tCCATTATACGACAAACCGGCGGCCCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGcgcg < 2:523683/149‑1 (MQ=255) cgcgccGGCGGCCCGGCGTTAGGGCTGATCTCGACTAGGTCTACTCCGGCTTCCTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTCAGCGAACTTCCTGGGCGCGAATTTCGCCAtt < 2:13850/145‑1 (MQ=255) gcggcTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCCTTGATACgg > 1:296230/1‑149 (MQ=255) cTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAg > 2:611468/1‑149 (MQ=255) tcGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGCTACGGTTCGGGCGCGCCGGTTGAACTCg > 2:219702/1‑149 (MQ=255) tAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATCCCAAGCTGCTCGCCTTCCAGACCTGTTAAGAGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTc < 1:502455/149‑1 (MQ=255) gTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACTCAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAACTTCGCCATTGATACGGTTCGGACGCGCCGTTTGAACTCGTTTTCCGc < 1:422208/149‑1 (MQ=255) tCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTAGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTaa > 2:97208/1‑149 (MQ=255) tCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTAGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTaa > 2:96798/1‑149 (MQ=255) ttCTCCCGAGCTTCTCCCAGACTCACAATCCCCAGCTGCTCGCCCTCCAGATCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCaa < 2:588439/149‑1 (MQ=255) agagCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGCACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGttt > 1:340633/1‑149 (MQ=255) gagCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTa < 1:40586/149‑1 (MQ=255) gccTCACAATTCCCCGCTGCTCGCCTTCCAGACCTGTTAAGCGCACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTg < 1:217096/147‑1 (MQ=255) cAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCGTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGGTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCttgt > 1:486613/1‑149 (MQ=255) cAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGATTGCGGCTGGCAATCTCttgt > 1:425232/1‑149 (MQ=255) acgctgctCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTACGAATTTAAGACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCttgtt < 1:333284/147‑1 (MQ=255) cGCCTTCCAGACCTGTTAAGCGAACTTCCTAGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTTTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTtc < 1:97208/149‑1 (MQ=255) cGCCTTCCAGACCTGTTAAGCGAACTTCCTAGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTtc < 1:96798/149‑1 (MQ=255) ttCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGa > 1:607886/1‑149 (MQ=255) gcacaCTTCCTGGGCGCGAACTTTGCCATTGATACGGTTAGTGCTCGCCGTTTGCTCTTGTTTTTCGCCTTTGTTTCCTCATCCCTCCGCGCTTTTTAGCCTGCGGCGGCGAATCTCTTGTTGCAGCTTCTCGCTCACCTCATTTACGt < 2:299104/145‑1 (MQ=255) ctaaCTTCCTGGCCGCGCATTTCGCCATTTATTCGGTTAGGGCGCGCCTTTTGAACTCGTTTTCCGCCTTTAATCCCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCTAATCTCTTGTTGCAGCTTCTCGATCACCTCATTTACgcc < 2:537876/147‑3 (MQ=255) gAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTGTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCACTTGTGGCAGCTTCTCGCCCACTTCATTTACGTca > 2:241398/1‑148 (MQ=255) | ATCCATTATACGACAAACCGGCGGCTCGGCGTTAGGGCTGATCTCGACTAAGTCTACTCCGGCTTCTTCTGCTTTCTCCAGAGCTTCTCTCAGACTCACAATACCAAGCTGCTCGCCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATCACTTCATTTACGTCC > NC_000913/1800432‑1800714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |