Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,834,598 C→T 10.0% A230V (GCG→GTG)  norW → NADH:flavorubredoxin oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,834,5980CT10.0% 100.3 / 3.4 40A230V (GCG→GTG) norWNADH:flavorubredoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (19/17);  new base T (2/2);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01

TGCCACCGGAAGTAAGCAGCCGCTTGCAGCATCGGTTGACGGAGATGGGCGTTCATCTGCTGTTGAAATCTCAGTTACAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGC  >  NC_000913/2834451‑2834733
                                                                                                                                                   |                                                                                                                                       
tGCCACCGGAAGTAAGCAGCCGCTTGCAGCATCGGTTGACGGAGATGGGCGTTCATCTGCTGTTGAAATCTCAGTTACAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGcc                                                                                                                                        >  2:98442/1‑148 (MQ=255)
 gCCACCGGAAGGAAGCAGCCGCTTGCACCATCCGTTGACGGAGATGGGCGTTCATCTGCTGTTGAAATCTCAGTTACAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCgg                                                                                                                                       <  1:3354/149‑1 (MQ=255)
         aaGTAAGCAGCCGGTTGCAGGATCGGTTGACGGAGATGGGCGTTCATCTGCTGTTGAAATCTCAGTTACAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTgcc                                                                                                                               <  2:648663/149‑1 (MQ=255)
         aaGTAAGCAGCCGCTTGCAGCAGCGGTTGACTGAGATCGCAGCTCACCTGCTGTCGAGCGCGCAGTTACAGGAGCTCGAAAAAACTGACGCTGCAATTCATTCGACGCCGGACCGTCATCGTACGATCGAACTGGTTGCTGTacccgcc                                                                                                                               <  2:254968/149‑7 (MQ=255)
             aaGCAGCCGCTTGCAGCATCGGTTGACGGAGATGGGCGTTCATCTGCTGTTGAAATCTCAGTTACAGGGGCTGGAAAAAACGGATTTTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCa                                                                                                                           <  2:424743/149‑1 (MQ=255)
              agcagcCGCTTGCAGCATCGGTTGACGGAGCTGGGCGTTCACCTGCTGTTGAAATCTCTGTTACATGGGCTGGCGAAGACGGATTCTGGCATTCAGGCAACGCAGGGCCGCCAGCGCAATGTCGCAGTGGCTGCGGTCTTTcccgcccc                                                                                                                          >  2:294414/1‑147 (MQ=255)
              agcagcCGCTTGCAGCATCGGTTGACGGAGATGGGCGTTCATCTGCTGTTGAAATCTCAGCTACAGGGGCTGGAAAAAACGGATTCTGGCCTTCAGGCAACGCTGGACCGCCGGCGCAATATCGGAGGGGATGCGGGACTTGCCGCCAc                                                                                                                          >  2:587607/1‑149 (MQ=255)
                        tGCAGCATCGGTTGACGGAGATGGGCGTTCATCTGCTGTTGAAATCTCAGTTACAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAAGATCGAAGTGGATGCGGTAATGGCCGCCACCGGGCTgcgc                                                                                                                >  2:599776/1‑149 (MQ=255)
                        tGCAGCATCGGTTGACGGAGATGGGCGTTCATCTGCTGTTGAAACCTCAGTTACAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTgcgc                                                                                                                >  2:599592/1‑149 (MQ=255)
                          cagcaTCGGTTGACGGAGATGGGCGTTCATCTGCTGTTGAAATCTCAGTTACAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGGTGCGGTAATTGCCGCCACCGGACTGCGccc                                                                                                              <  1:671063/149‑1 (MQ=255)
                           agcaTCGGTTGACGGAGATGGGCGTTCATCTGCTGTTGAAATCTCAGTTACAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCGTCGCGATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCg                                                                                                             >  1:145359/1‑149 (MQ=255)
                              aTCGGTTGACGGAGATGGGCGTTCATCTGCTGTTGAAATCTCAGTTACAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGaa                                                                                                          <  1:413425/149‑1 (MQ=255)
                                      aCGGAGATGGGCGTTCATCTGCTGTTGAAATCTCAGTTACAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCt                                                                                                  >  1:123026/1‑149 (MQ=255)
                                                   ttCATCTGCTGTTGAAATCTCAGTTACAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGcc                                                                                     >  1:354070/1‑149 (MQ=255)
                                                         tgctgTTGAAATCTCAGTTACAGGGACAAAAAGAAACTGATTCTGGCATTCAGGCAACGCTGAACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTg                                                                               >  1:339527/1‑149 (MQ=255)
                                                           ctgTTGAAATCTCAGTTACAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGTGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGAc                                                                             >  2:581099/1‑149 (MQ=255)
                                                           ctgTTGAAATCTCAGTTACAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGTGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGAc                                                                             >  2:238872/1‑149 (MQ=255)
                                                                   aTCTCAGTTACAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATc                                                                     >  1:35421/1‑149 (MQ=255)
                                                                     ctcAGTTACAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATcgc                                                                   >  1:293229/1‑149 (MQ=255)
                                                                           tACAGGGGCTGTAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGtt                                                             <  1:637366/149‑1 (MQ=255)
                                                                            aCAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGttt                                                            <  1:123126/149‑1 (MQ=255)
                                                                            aCAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGttt                                                            <  2:485328/149‑1 (MQ=255)
                                                                             cAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTg                                                           >  1:623781/1‑149 (MQ=255)
                                                                                gggCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGCGACGACGCGCCGGGCTGACGATTAATCACGGCGGTTGCGt                                                        >  1:191541/1‑149 (MQ=255)
                                                                                 ggCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTc                                                       <  1:558854/149‑1 (MQ=255)
                                                                                    tGGAAAAAACGGATTCTGGCATTCAGGCAACGCGGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCACTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGAt                                                    <  1:565918/149‑1 (MQ=255)
                                                                                            aCGGATTCTGGCATTCAGGCAACGCTGGCCCGCCAGCGCACTATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCt                                            <  2:145359/149‑1 (MQ=255)
                                                                                                  tCTGGCATTCAGGCAACGCTGGACCGCGAGCGCAATATGGAAGTGGATGCGGTAATTGCCGCCAACGGCGTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGCCGTTTGCGTCGATAGTTAACTGCaaaa                                      >  1:315760/1‑148 (MQ=255)
                                                                                                    tGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGTGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCa                                    <  1:650694/149‑1 (MQ=255)
                                                                                                       catcccggCAACGCTGGACCGCCAGCGCAAGATCGAAGGGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTa                                 <  2:259197/143‑1 (MQ=255)
                                                                                                       cATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGTGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTa                                 <  1:238872/149‑1 (MQ=255)
                                                                                                       cATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGTGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTa                                 <  1:581099/149‑1 (MQ=255)
                                                                                                        aTTCAGGCAACGCTGGACCGCCATCGCAGTATCGCAGTGGATGCGGTAATTGCGGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTaa                                >  1:382367/1‑149 (MQ=255)
                                                                                                         ttCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGCAACCGCCCTGGCCCGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGGCGATAGTTCTCTGCAAACCacgaat                               >  1:264363/1‑144 (MQ=255)
                                                                                                              gCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGa                          <  2:293229/149‑1 (MQ=255)
                                                                                                                    cTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGACGTCGTGGAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTa                    >  1:504145/1‑149 (MQ=255)
                                                                                                                       gACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTAcgc                 >  2:369999/1‑149 (MQ=255)
                                                                                                                                cgcAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGa        >  1:554916/1‑149 (MQ=255)
                                                                                                                                    atagCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTgc    <  1:98442/149‑1 (MQ=255)
                                                                                                                                     tatCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTgcg   >  1:654818/1‑149 (MQ=255)
                                                                                                                                      atCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACGGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTGTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTgcgc  <  2:123026/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                       
TGCCACCGGAAGTAAGCAGCCGCTTGCAGCATCGGTTGACGGAGATGGGCGTTCATCTGCTGTTGAAATCTCAGTTACAGGGGCTGGAAAAAACGGATTCTGGCATTCAGGCAACGCTGGACCGCCAGCGCAATATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAAACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGC  >  NC_000913/2834451‑2834733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑