Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,223,086 G→T 6.3% K152N (AAG→AAT)  ebgA → evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,223,0860GT 6.3% 175.8 / 2.1 64K152N (AAG→AAT) ebgAevolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (31/29);  new base T (2/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

GTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATG  >  NC_000913/3222940‑3223231
                                                                                                                                                  |                                                                                                                                                 
gTCCCCCGCGATAACCCCACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTTATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTTGGTTTCAGCAAggg                                                                                                                                                 <  1:187751/149‑1 (MQ=255)
gTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGGTTATGTTAACGGTCAGTATGGGGGTTTCAGGACggg                                                                                                                                                 >  2:184798/1‑149 (MQ=255)
   cccAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGCCGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAg                                                                                                                                              >  2:13352/1‑149 (MQ=255)
    ccAGCGATAACCCAACCGGTGCCTATCACCGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTTACGGCGTCGAAACCTATTTTGAAGTCTTTGTTAGCGAACAGTATGTGGGTTTCAGCTAGGGCAGt                                                                                                                                             >  2:296563/1‑149 (MQ=255)
    ccAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTAACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGATTCAGCAAGGCCAGt                                                                                                                                             >  2:297379/1‑149 (MQ=255)
    ccAGCGATAACCCAACCGGTAACTATCAGCGTATTTCAACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGAGGAAAGTTGGCGGCGTCGAAACCTATTGTGAAGTCTATGTTAACGGTCAGGATGTGGGTTTCAGGAAGGGCAGt                                                                                                                                             >  2:648984/1‑149 (MQ=255)
       gCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGc                                                                                                                                          <  1:686931/149‑1 (MQ=255)
                 aaCCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAGCCTACTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGAAAGGGCAGTCGCCTGACCGCag                                                                                                                                <  2:687700/149‑1 (MQ=255)
                      gTGCCTATCAACGTATTTTCACGCTCAGCGACGGCTCGTAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAATCTATTTTGAAGTCTATGTTAACGGCCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGttt                                                                                                                           <  2:502786/149‑1 (MQ=255)
                      gTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGttt                                                                                                                           >  2:298900/1‑149 (MQ=255)
                             tCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCa                                                                                                                    >  1:267576/1‑149 (MQ=255)
                                aCGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAATCCTATTTTGAAGTCTATGTTAACGGTCAGGATGTGGGTTTCAGCACGGGCAGTCGCCTGACCGCAGACACTGACATCAgcg                                                                                                                 >  2:851603/1‑149 (MQ=255)
                                aCGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAgcg                                                                                                                 >  1:357208/1‑149 (MQ=255)
                                aCGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAgcg                                                                                                                 >  1:739596/1‑149 (MQ=255)
                                   tATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGTAGTCGCCTGACCGCACAGTTTGACATCCGCGCGa                                                                                                              >  2:188977/1‑149 (MQ=255)
                                     ttttCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATg                                                                                                            <  1:755148/149‑1 (MQ=255)
                                       ttCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGGTTGACATCAGCGCGATGGt                                                                                                          >  1:422845/1‑149 (MQ=255)
                                        tCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGtt                                                                                                         <  1:552542/149‑1 (MQ=255)
                                           cccTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTaaa                                                                                                      <  1:154103/149‑1 (MQ=255)
                                             cTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAAc                                                                                                    <  1:774694/149‑1 (MQ=255)
                                                   gACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACCTAAGCGCGATGGTTAAAACCGGcca                                                                                              >  2:771017/1‑147 (MQ=255)
                                                      ggCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGGCag                                                                                           >  1:422866/1‑148 (MQ=255)
                                                        cTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAAcc                                                                                         >  1:816863/1‑149 (MQ=255)
                                                           gCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTACCGGTCAGTATGTGGGGTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCtgt                                                                                      >  1:752229/1‑149 (MQ=255)
                                                            cAGGGTAAACAGGCGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGATGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCtgtt                                                                                     <  2:636708/149‑1 (MQ=255)
                                                            cAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTCTGACATCAGCGCGATGGTTAAAACCGGCGACAACCtgtt                                                                                     <  2:735942/149‑1 (MQ=255)
                                                               ggTAAACAGACGCTGATTAAATTGGACGGCGGCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGCGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTtgtg                                                                                  <  2:685896/149‑1 (MQ=255)
                                                                ggaaaCAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTtgtgt                                                                                 <  2:652845/147‑1 (MQ=255)
                                                                gTAAACGGCCGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTtgtgt                                                                                 <  1:321414/149‑1 (MQ=255)
                                                                gTAAACAGACGATGATTAATTTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTtgtgt                                                                                 <  2:281916/149‑1 (MQ=255)
                                                                 tAAACAGACGCTGATTAAAGTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTACAGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTtgtgtg                                                                                <  1:411804/149‑1 (MQ=255)
                                                                  aaaCAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTtgtgtgt                                                                               <  1:146580/149‑1 (MQ=255)
                                                                    aCAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTgc                                                                             <  1:785380/149‑1 (MQ=255)
                                                                      aGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTgcgc                                                                           >  1:643633/1‑149 (MQ=255)
                                                                          gCTGATTAAATTTGACGGCGTCGAACCCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGa                                                                       <  1:13352/149‑1 (MQ=255)
                                                                              aTTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTAAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCa                                                                   >  1:669965/1‑149 (MQ=255)
                                                                                 aaaTTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAGCCTGTTGTGTGTGCGCGGGATGCAGTg                                                                >  1:35093/1‑149 (MQ=255)
                                                                                   aTTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGCCAACCTGTTGGGTGTGCGCGTGATGCAGTggg                                                              >  1:1700/1‑149 (MQ=255)
                                                                                    ttgtaCGGGGTCGAAACCGATTTTGAAGTCGATGTTAACGGTCAGTAGATGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCTTGATGCAGTGGGc                                                             <  2:523307/145‑1 (MQ=255)
                                                                                     ttGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGCCATCAGCGCGATGGTTAACACCGGCGACAACCTGTTGTGTGTGCGCGGGATGCGGTGGGCg                                                            >  2:651267/1‑149 (MQ=255)
                                                                                      tGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAATGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCgg                                                           >  1:259347/1‑149 (MQ=255)
                                                                                      tGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAATGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCgg                                                           >  1:259236/1‑149 (MQ=255)
                                                                                         gggggTCGTAGCCTATTTTGAGGTCTATGTTACCGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAGCCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTAcg                                                        <  2:361265/145‑2 (MQ=255)
                                                                                             gTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTctac                                                    >  1:789895/1‑149 (MQ=255)
                                                                                             gTCGAAACCTACTTTGAAGTCTATTTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTctac                                                    <  1:723448/149‑1 (MQ=255)
                                                                                               cGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGGTTGACATCAGCGCGAGGGTTAAAAACGACGGCCACCTGTTGTGTGTGCGACAGATGCAGTGGGCGGGCTcgactt                                                  >  2:560355/1‑147 (MQ=255)
                                                                                                      gaTTTTGGCGTCTATGTTAACGGTCAGTATGTGGGTTGCAGCAAGGGCAATGGCCTGACCGCAGTGCTTGACCTGTCCGCGTTGGGTAAAGCTGGCGACAACCTGTTGCGTGTTCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGa                                           <  2:561222/148‑1 (MQ=255)
                                                                                                            gAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGTGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGAcgg                                     >  2:48194/1‑147 (MQ=255)
                                                                                                             aaGGCTGTGTTAACGGTCAGTATGTGGGTTGCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAg                                    <  2:306789/149‑1 (MQ=255)
                                                                                                                tCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGac                                 >  2:162602/1‑148 (MQ=255)
                                                                                                                    tGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATAtgt                             <  2:267576/149‑1 (MQ=255)
                                                                                                                      ttAACGGTCAGTTAGGGGGTTTCAGCAAGGGCAGTCGCCTTACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATgtgg                           <  2:669965/149‑1 (MQ=255)
                                                                                                                       tAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATgtggt                          <  1:682823/149‑1 (MQ=255)
                                                                                                                        aaCGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACCACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATgtggtg                         >  1:600040/1‑149 (MQ=255)
                                                                                                                           ggTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCGGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATAGGTGGTGGTc                      >  2:124297/1‑149 (MQ=255)
                                                                                                                             tCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGGTGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTCCGTGGAAGACACGGATATGTGGTGGGCAg                    >  2:18903/1‑149 (MQ=255)
                                                                                                                                 tATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCATTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCggg                <  1:589438/149‑1 (MQ=255)
                                                                                                                                    gtgtgtTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGAt             <  2:299949/149‑1 (MQ=255)
                                                                                                                                    gtgGGTTTCAGCAAGGGCAGGCGCCTGCCCGCGGAGTTTGTCATCAGCGCGATGGTTAAAGCCGGCGACAACCTGTTGGGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGAt             <  2:368789/149‑1 (MQ=255)
                                                                                                                                     tgGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATc            <  1:298900/149‑1 (MQ=255)
                                                                                                                                       ggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCtt          >  1:212754/1‑149 (MQ=255)
                                                                                                                                          ttCAGCAATGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCAGGGATCTTCcg       <  2:259236/149‑1 (MQ=255)
                                                                                                                                          ttCAGCAATGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCAGGGATCTTCcg       <  2:259347/149‑1 (MQ=255)
                                                                                                                                           tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcgc      >  1:132550/1‑149 (MQ=255)
                                                                                                                                             aGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGTGATGTGGTGGTCAGCGGGGATCTTCCGCGa    >  2:582594/1‑149 (MQ=255)
                                                                                                                                               cAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATg  >  2:20185/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATG  >  NC_000913/3222940‑3223231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑